Strain Name:

C3HeB.129S7-Kcna1tm1Tem/J

Stock Number:

003532

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names C3HeB.129S7(B6)-Kcna1tm1Tem/J    (Changed: 08-MAY-06 )
Type Congenic; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C3HeB/FeJ
Donor Strain B6,129S-Kcna1tm1Tem (129S7 derived AB1 ES cell line(+Hprt-bm2))
 
Donating InvestigatorDr. Bruce L. Tempel,   Univ of Washington School of Medicine

Description
Homozygous null mice display an epileptic phenotype (episodic eye blinking, twitching of vibrissae, forelimb paddling, arrested motion, hyperstartle response) beginning during the third postnatal week. Quantitative RNase protection analysis on brain tissue indicates that in heterozygous mice the Kcna1 transcript is reduced to approximately half that observed in wild-type littermates. No transcripts were detected in homozygous mice. Approximately 50% of the homozygous mice die between the third and fifth weeks of life. Mice surviving this period survive for varying periods, depending on genetic background; congenic C3HeB/FeJ mice usually die at 6-8 weeks; hybrid N:NIHS-BC mice have been maintained for over 12 months during which they continue to display spontaneous seizures. A similar phenotype with spontaneous seizures has been observed in mice having 129/Sv, C3HeB/FeJ, C57BL/6 X 129/Sv, and 129/Sv X N:NIHS-BC backgrounds.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt the open reading frame. The construct was electroporated into 129S7/SvEvBrd-Hprt+ derived AB1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C3HeB/FeJ mice, and then backcrossed to the same for 15 generations.

Control Information

  Control
   Wild-type from the colony
   000658 C3HeB/FeJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Kcna1
002434   B6 x BALB/cByJ-Kcna1mceph/J
View Strains carrying other alleles of Kcna1     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Episodic Ataxia, Type 1; EA1   (KCNA1)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Kcna1tm1Tem/Kcna1tm1Tem

        C3Fe.129S7-Kcna1tm1Tem
  • behavior/neurological phenotype
  • decreased chemical nociceptive threshold
    • mutants display greater pain behavior in response to formalin injection; total paw licking time is greater in mutants   (MGI Ref ID J:107933)
  • decreased chemically-elicited antinociception
    • effects of subcutaneous morphine is attenuated in mutants compared to wild-type, as assessed by increase in latency in the thermal pain assays   (MGI Ref ID J:107933)
  • decreased thermal nociceptive threshold
    • mutant animals show reduced latencies in 2 thermal pain assays; in response to a focused light beam on the hind paw and in a hot plate assay, mutants more quickly display a paw flick or paw lick, respectively   (MGI Ref ID J:107933)
  • nervous system phenotype
  • abnormal nervous system electrophysiology
    • low voltage activated K+ currents are significantly smaller than controls   (MGI Ref ID J:105296)
    • abnormal action potential
      • action potentials are generated to very small current clamp steps   (MGI Ref ID J:105296)
      • threshold about 48 pA as opposed to 90pA for controls   (MGI Ref ID J:105296)
      • two times as many action potentials generated as by controls   (MGI Ref ID J:105296)
    • decreased nerve fiber response threshold   (MGI Ref ID J:105296)
  • integument phenotype
  • decreased chemical nociceptive threshold
    • mutants display greater pain behavior in response to formalin injection; total paw licking time is greater in mutants   (MGI Ref ID J:107933)
  • decreased chemically-elicited antinociception
    • effects of subcutaneous morphine is attenuated in mutants compared to wild-type, as assessed by increase in latency in the thermal pain assays   (MGI Ref ID J:107933)
  • decreased thermal nociceptive threshold
    • mutant animals show reduced latencies in 2 thermal pain assays; in response to a focused light beam on the hind paw and in a hot plate assay, mutants more quickly display a paw flick or paw lick, respectively   (MGI Ref ID J:107933)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Kcna1tm1Tem/Kcna1+

        involves: 129S7/SvEvBrd * various
  • nervous system phenotype
  • increased susceptibility to pharmacologically induced seizures
    • exposure to flurothyl induces seizure at a latency of 3.39+/-0.08 minutes (a 9% reduction compared to wild-type mice, 3.72+/.-0.07 minutes)   (MGI Ref ID J:47349)
  • behavior/neurological phenotype
  • increased susceptibility to pharmacologically induced seizures
    • exposure to flurothyl induces seizure at a latency of 3.39+/-0.08 minutes (a 9% reduction compared to wild-type mice, 3.72+/.-0.07 minutes)   (MGI Ref ID J:47349)

Kcna1tm1Tem/Kcna1tm1Tem

        involves: 129S7/SvEvBrd * various
  • mortality/aging
  • premature death
    • 50% of mice die suddenly between the third and fifth week of life   (MGI Ref ID J:47349)
  • nervous system phenotype
  • abnormal CNS synaptic transmission
    • 21% of mossy fiber cells elicit a late burst discharge when stimulated that is not observed in wild-type cells   (MGI Ref ID J:47349)
    • the threshold for eliciting antidronic spike invasion is decreased (10.9+/-1.5 uA compared to 18.5+/-2.9 uA in wild-type CA3 pyramidal cells)   (MGI Ref ID J:47349)
    • however, initial fast excitatory postsynaptic potential, fast inhibitory postsynaptic potential and slow inhibitory postsynptical potential are normal   (MGI Ref ID J:47349)
  • abnormal action potential
    • compound action potential is slower to repolarize that in wild-type mice   (MGI Ref ID J:47349)
  • abnormal brain wave pattern
    • mice exhibit ictal electrocortical patterns that are usually associated with ictal behaviors   (MGI Ref ID J:47349)
  • abnormal paired-pulse facilitation
    • the refractory period in the sciatic nerve is increased after the second pulse   (MGI Ref ID J:47349)
  • seizures
    • some mice exhibit seizures prior to dying   (MGI Ref ID J:47349)
    • mice that survive beyond 5 weeks of age exhibit spontaneous seizures lasting 20 seconds to 2 minutes once or twice an hour   (MGI Ref ID J:47349)
    • ictal behaviors include circling, facial clonus, rearing and falling, bilateral and alternating forelimb or hindlimb clonus or both and generalized tonic-clonic episodes   (MGI Ref ID J:47349)
    • increased susceptibility to pharmacologically induced seizures
      • exposure to flurothyl induces seizure at a latency of 1.48+/-0.18 minutes (a 60% reduction compared to wild-type mice, 3.72+/.-0.07 minutes)   (MGI Ref ID J:47349)
    • tonic-clonic seizures   (MGI Ref ID J:47349)
  • behavior/neurological phenotype
  • abnormal behavior
    • during postnatal week 3, mice exhibit episodic eye blinking, twitching of vibrissiae, forelimb padding, arrested motion, and hyperstartle response   (MGI Ref ID J:47349)
    • ictal behaviors include circling, facial clonus, rearing and falling, bilateral and alternating forelimb or hindlimb clonus or both and generalized tonic-clonic episodes   (MGI Ref ID J:47349)
    • increased blinking frequency
      • during postnatal week 3   (MGI Ref ID J:47349)
    • increased startle reflex
      • during postnatal week 3   (MGI Ref ID J:47349)
    • seizures
      • some mice exhibit seizures prior to dying   (MGI Ref ID J:47349)
      • mice that survive beyond 5 weeks of age exhibit spontaneous seizures lasting 20 seconds to 2 minutes once or twice an hour   (MGI Ref ID J:47349)
      • ictal behaviors include circling, facial clonus, rearing and falling, bilateral and alternating forelimb or hindlimb clonus or both and generalized tonic-clonic episodes   (MGI Ref ID J:47349)
      • increased susceptibility to pharmacologically induced seizures
        • exposure to flurothyl induces seizure at a latency of 1.48+/-0.18 minutes (a 60% reduction compared to wild-type mice, 3.72+/.-0.07 minutes)   (MGI Ref ID J:47349)
      • tonic-clonic seizures   (MGI Ref ID J:47349)

Kcna1tm1Tem/Kcna1tm1Tem

        involves: 129S7/SvEvBrd * C57BL/6
  • behavior/neurological phenotype
  • ataxia
    • mutants exhibit ataxic movemensts as they begin to walk following swim test recovery   (MGI Ref ID J:77281)
  • impaired balance
    • mutants removed from the cold water bath after the swim test and placed on a dry platform fall onto their sides and show severe myotonia   (MGI Ref ID J:77281)
  • impaired swimming
    • in cold water at the end of a 2 minute period, mutant mice start to have difficulties in maintaining axial orientation while wild-type controls are unaffected   (MGI Ref ID J:77281)
  • seizures
    • after the swim test, one mutant showed epileptic neural activity 7 minutes after the swim   (MGI Ref ID J:77281)
  • tremors
    • after the swim test, mutants fall over and all limbs undergo violent tremors; tremors lessen as time progresses   (MGI Ref ID J:77281)
  • nervous system phenotype
  • abnormal PNS synaptic transmission
    • upon cooling of phrenic nerve-diaphragm preparations, the mutant preparation exhibits delayed repetitive discharge after a single nerve stimulation (hyperexcitability) ; the wild-type NM transmission remains one-to-one irrespective of temperature   (MGI Ref ID J:77281)
  • seizures
    • after the swim test, one mutant showed epileptic neural activity 7 minutes after the swim   (MGI Ref ID J:77281)

Kcna1tm1Tem/Kcna1tm1Tem

        involves: 129S7SvEvBrd * BALB/cByJ * C3HeB/FeJ
  • nervous system phenotype
  • abnormal cerebral cortex morphology
    • enlarged ventral cortex in 69% of brains   (MGI Ref ID J:119491)
  • convulsive seizures
    • 2/3 show class IV seizures and half of those show class V seizures   (MGI Ref ID J:119491)
  • enlarged hippocampus
    • in 69% of brains   (MGI Ref ID J:119491)
  • behavior/neurological phenotype
  • abnormal locomotor behavior
    • abnormal limb paddling   (MGI Ref ID J:119491)
  • abnormal posture   (MGI Ref ID J:119491)
  • convulsive seizures
    • 2/3 show class IV seizures and half of those show class V seizures   (MGI Ref ID J:119491)
  • increased startle reflex
    • hyperstartle response   (MGI Ref ID J:119491)
  • tremors
  • hearing/vestibular/ear phenotype
  • abnormal hearing physiology
    • abnormal audiosensitivity   (MGI Ref ID J:119491)
  • ear inflammation
    • crusting in the outer ear   (MGI Ref ID J:119491)
  • vision/eye phenotype
  • eye inflammation
    • crusting of the eyelid   (MGI Ref ID J:119491)
  • homeostasis/metabolism phenotype
  • porphyria   (MGI Ref ID J:119491)
  • immune system phenotype
  • ear inflammation
    • crusting in the outer ear   (MGI Ref ID J:119491)
  • eye inflammation
    • crusting of the eyelid   (MGI Ref ID J:119491)

Kcna1tm1Tem/Kcna1tm1Tem

        involves: 129S7/SvEvBrd
  • mortality/aging
  • partial postnatal lethality
    • die beginning at 2-3 weeks of age   (MGI Ref ID J:164091)
  • premature death
    • die beginning at 2-3 weeks of age   (MGI Ref ID J:164091)
  • behavior/neurological phenotype
  • seizures
    • develop epilepsy   (MGI Ref ID J:164091)
    • seizures exacerbate cardiac abnormalities   (MGI Ref ID J:164091)
    • myoclonus
      • myoclonus that corresponds to prolonged bradycardia   (MGI Ref ID J:164091)
    • tonic-clonic seizures   (MGI Ref ID J:164091)
  • cardiovascular system phenotype
  • abnormal cardiovascular system physiology
    • mice exhibit interictal cardiac abnormalities   (MGI Ref ID J:164091)
    • abnormal impulse conducting system conduction
      • mice exhibit multiple patterns of functional cardiac rhythm disturbances   (MGI Ref ID J:164091)
      • atrioventricular block
        • 5-fold increase in AV conduction blocks compared to controls   (MGI Ref ID J:164091)
        • most common AV block is type I, second-degree block characterized by a PR interval that progressively lengthened until the QRS complex was dropped   (MGI Ref ID J:164091)
        • less often, type 2, second-degree blocks are seen in which the heart skipped one or more beats without obvious PR lengthening   (MGI Ref ID J:164091)
    • decreased heart rate
      • prolonged bradycardia, with extended periods of sinus bradycardia lasting many minutes   (MGI Ref ID J:164091)
      • however, overall basal heart rate is similar to wild-type, although the heart rate is more variable than in controls   (MGI Ref ID J:164091)
    • ventricular premature beat
      • excessive premature ventricular contractions   (MGI Ref ID J:164091)
  • muscle phenotype
  • myoclonus
    • myoclonus that corresponds to prolonged bradycardia   (MGI Ref ID J:164091)
  • nervous system phenotype
  • seizures
    • develop epilepsy   (MGI Ref ID J:164091)
    • seizures exacerbate cardiac abnormalities   (MGI Ref ID J:164091)
    • myoclonus
      • myoclonus that corresponds to prolonged bradycardia   (MGI Ref ID J:164091)
    • tonic-clonic seizures   (MGI Ref ID J:164091)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Kcna1tm1Tem related

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Channel and Transporter Defects
Genes Regulating Growth and Proliferation

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Channel and Transporter Defects
      potassium
Epilepsy
Hearing Defects

Sensorineural Research
Hearing Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Kcna1tm1Tem
Allele Name targeted mutation 1, Bruce L Tempel
Allele Type Targeted (knock-out)
Common Name(s) Kv1.1 null; Kv1.1-;
Mutation Made ByDr. Bruce Tempel,   Univ of Washington School of Medicine
Strain of Origin129S7/SvEvBrd-Hprt<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt<+>
Gene Symbol and Name Kcna1, potassium voltage-gated channel, shaker-related subfamily, member 1
Chromosome 6
Gene Common Name(s) AEMK; AI840627; EA1; HBK1; HUK1; K+ channel, A current, subtype 1, gene 1, brain; KV1.1; Kca1-1; Kcna; Kcpvd; MBK1; MK1; Mk-1; RBK1; Shak; brain K+ channel transient (A current) subtype 1; expressed sequence AI840627; mceph; megencephaly; mouse K+ channel gene 1 (brain, homolog to Drosophila Shaker); mouse brain potassium channel protein-1;
General Note Phenotypic Similarity to Human Syndrome: sudden unexplained death in epilepsy (SUDEP) (J:164091).
Molecular Note A neomycin resistance cassette replaced the entire coding region of the gene. RNA from the deleted gene was undetectable by quantitative RNase protection assays of brain tissue from homozygous null mice. [MGI Ref ID J:47349]

Genotyping

Genotyping Information

Genotyping Protocols

Kcna1tm1Tem-Alternate 1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Smart SL; Lopantsev V; Zhang CL; Robbins CA; Wang H; Chiu SY ; Schwartzkroin PA ; Messing A ; Tempel BL. 1998. Deletion of the K(V)1.1 potassium channel causes epilepsy in mice. Neuron 20(4):809-19 HUMAN. [PubMed: 9581771]  [MGI Ref ID J:47349]

Additional References

Frankel WN; Taylor L; Beyer B; Tempel BL; White HS. 2001. Electroconvulsive thresholds of inbred mouse strains. Genomics 74(3):306-12. [PubMed: 11414758]  [MGI Ref ID J:70226]

Kopp-Scheinpflug C; Fuchs K; Lippe WR; Tempel BL; Rubsamen R. 2003. Decreased temporal precision of auditory signaling in Kcna1-null mice: an electrophysiological study in vivo. J Neurosci 23(27):9199-207. [PubMed: 14534254]  [MGI Ref ID J:88201]

van Brederode JF; Rho JM; Cerne R; Tempel BL; Spain WJ. 2001. Evidence of altered inhibition in layer V pyramidal neurons from neocortex of Kcna1-null mice. Neuroscience 103(4):921-9. [PubMed: 11301201]  [MGI Ref ID J:85908]

Kcna1tm1Tem related

Allen PD; Ison JR. 2012. Kcna1 gene deletion lowers the behavioral sensitivity of mice to small changes in sound location and increases asynchronous brainstem auditory evoked potentials but does not affect hearing thresholds. J Neurosci 32(7):2538-43. [PubMed: 22396426]  [MGI Ref ID J:181606]

Allen PD; Schmuck N; Ison JR; Walton JP. 2008. Kv1.1 channel subunits are not necessary for high temporal acuity in behavioral and electrophysiological gap detection. Hear Res 246(1-2):52-58. [PubMed: 18926893]  [MGI Ref ID J:143590]

Baraban SC; Southwell DG; Estrada RC; Jones DL; Sebe JY; Alfaro-Cervello C; Garcia-Verdugo JM; Rubenstein JL; Alvarez-Buylla A. 2009. Reduction of seizures by transplantation of cortical GABAergic interneuron precursors into Kv1.1 mutant mice. Proc Natl Acad Sci U S A 106(36):15472-7. [PubMed: 19706400]  [MGI Ref ID J:153095]

Brew HM; Hallows JL; Tempel BL. 2003. Hyperexcitability and reduced low threshold potassium currents in auditory neurons of mice lacking the channel subunit Kv1.1. J Physiol 548(Pt 1):1-20. [PubMed: 12611922]  [MGI Ref ID J:105296]

Chen G; Gao W; Reinert KC; Popa LS; Hendrix CM; Ross ME; Ebner TJ. 2005. Involvement of kv1 potassium channels in spreading acidification and depression in the cerebellar cortex. J Neurophysiol 94(2):1287-98. [PubMed: 15843481]  [MGI Ref ID J:127985]

Clark JD; Tempel BL. 1998. Hyperalgesia in mice lacking the Kv1.1 potassium channel gene. Neurosci Lett 251(2):121-4. [PubMed: 9718989]  [MGI Ref ID J:107933]

Fisahn A; Lavebratt C; Canlon B. 2011. Acoustic startle hypersensitivity in Mceph mice and its effect on hippocampal excitability. Eur J Neurosci 34(7):1121-30. [PubMed: 21966978]  [MGI Ref ID J:178006]

Glasscock E; Qian J; Yoo JW; Noebels JL. 2007. Masking epilepsy by combining two epilepsy genes. Nat Neurosci 10(12):1554-8. [PubMed: 17982453]  [MGI Ref ID J:130784]

Glasscock E; Yoo JW; Chen TT; Klassen TL; Noebels JL. 2010. Kv1.1 potassium channel deficiency reveals brain-driven cardiac dysfunction as a candidate mechanism for sudden unexplained death in epilepsy. J Neurosci 30(15):5167-75. [PubMed: 20392939]  [MGI Ref ID J:164091]

Holth JK; Bomben VC; Reed JG; Inoue T; Younkin L; Younkin SG; Pautler RG; Botas J; Noebels JL. 2013. Tau loss attenuates neuronal network hyperexcitability in mouse and Drosophila genetic models of epilepsy. J Neurosci 33(4):1651-9. [PubMed: 23345237]  [MGI Ref ID J:193896]

Jiang X; Zhang YH; Clark JD; Tempel BL; Nicol GD. 2003. Prostaglandin E2 inhibits the potassium current in sensory neurons from hyperalgesic Kv1.1 knockout mice. Neuroscience 119(1):65-72. [PubMed: 12763069]  [MGI Ref ID J:126732]

Karcz A; Hennig MH; Robbins CA; Tempel BL; Rubsamen R; Kopp-Scheinpflug C. 2011. Low-voltage activated Kv1.1 subunits are crucial for the processing of sound source location in the lateral superior olive in mice. J Physiol 589(Pt 5):1143-57. [PubMed: 21224222]  [MGI Ref ID J:183138]

Kopp-Scheinpflug C; Fuchs K; Lippe WR; Tempel BL; Rubsamen R. 2003. Decreased temporal precision of auditory signaling in Kcna1-null mice: an electrophysiological study in vivo. J Neurosci 23(27):9199-207. [PubMed: 14534254]  [MGI Ref ID J:88201]

Lopantsev V; Tempel BL; Schwartzkroin PA. 2003. Hyperexcitability of CA3 pyramidal cells in mice lacking the potassium channel subunit Kv1.1. Epilepsia 44(12):1506-12. [PubMed: 14636320]  [MGI Ref ID J:103036]

Ma Z; Lavebratt C; Almgren M; Portwood N; Forsberg LE; Branstrom R; Berglund E; Falkmer S; Sundler F; Wierup N; Bjorklund A. 2011. Evidence for presence and functional effects of Kv1.1 channels in beta-cells: general survey and results from mceph/mceph mice. PLoS One 6(4):e18213. [PubMed: 21483673]  [MGI Ref ID J:172237]

Persson AS; Klement G; Almgren M; Sahlholm K; Nilsson J; Petersson S; Arhem P; Schalling M; Lavebratt C. 2005. A truncated Kv1.1 protein in the brain of the megencephaly mouse: expression and interaction. BMC Neurosci 6:65. [PubMed: 16305740]  [MGI Ref ID J:104474]

Persson AS; Westman E; Wang FH; Khan FH; Spenger C; Lavebratt C. 2007. Kv1.1 null mice have enlarged hippocampus and ventral cortex. BMC Neurosci 8:10. [PubMed: 17250763]  [MGI Ref ID J:119491]

Rho JM; Szot P; Tempel BL; Schwartzkroin PA. 1999. Developmental seizure susceptibility of kv1.1 potassium channel knockout mice Dev Neurosci 21(3-5):320-7. [PubMed: 10575255]  [MGI Ref ID J:59642]

Simeone KA; Matthews SA; Samson KK; Simeone TA. 2014. Targeting deficiencies in mitochondrial respiratory complex I and functional uncoupling exerts anti-seizure effects in a genetic model of temporal lobe epilepsy and in a model of acute temporal lobe seizures. Exp Neurol 251:84-90. [PubMed: 24270080]  [MGI Ref ID J:206598]

Wenzel HJ; Vacher H; Clark E; Trimmer JS; Lee AL; Sapolsky RM; Tempel BL; Schwartzkroin PA. 2007. Structural consequences of Kcna1 gene deletion and transfer in the mouse hippocampus. Epilepsia 48(11):2023-46. [PubMed: 17651419]  [MGI Ref ID J:147685]

Zhang CL; Messing A; Chiu SY. 1999. Specific alteration of spontaneous GABAergic inhibition in cerebellar purkinje cells in mice lacking the potassium channel Kv1. 1. J Neurosci 19(8):2852-64. [PubMed: 10191303]  [MGI Ref ID J:54087]

Zhou L; Messing A; Chiu SY. 1999. Determinants of excitability at transition zones in Kv1.1-deficient myelinated nerves. J Neurosci 19(14):5768-81. [PubMed: 10407018]  [MGI Ref ID J:110896]

Zhou L; Zhang CL; Messing A; Chiu SY. 1998. Temperature-sensitive neuromuscular transmission in Kv1.1 null mice: role of potassium channels under the myelin sheath in young nerves. J Neurosci 18(18):7200-15. [PubMed: 9736643]  [MGI Ref ID J:77281]

van Brederode JF; Rho JM; Cerne R; Tempel BL; Spain WJ. 2001. Evidence of altered inhibition in layer V pyramidal neurons from neocortex of Kcna1-null mice. Neuroscience 103(4):921-9. [PubMed: 11301201]  [MGI Ref ID J:85908]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Low fecundity is observed among homozygous mating pairs. Expected coat color from breeding:Agouti

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

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Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000658 C3HeB/FeJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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