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Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Donating Investigator Dan Dumont, Sunnybrook Health Center Appearance
white-bellied agouti
Related Genotype: Aw/?
black
Related Genotype: a/aDescription
Inpp5d (commonly called SHIP) -deficient mice are viable and fertile. However, fertility rate is 10% lower in homozygotes and lifespan is shortened in comparison to controls. Inpp5d -deficient mice exhibit dramatic chronic hyperplasia of myeloid cells resulting in splenomegaly, lymphadenopathy and myeloid infiltration of vital organs. Histological and flow studies demonstrate a substantial increase in granulocyte-macrophage/monocyte populations in bone marrow, spleen and lymph nodes. Spleens and lymph nodes are severely enlarged compared to wildtype. The difference in spleen size is apparent as early as 4 weeks of age, and by 16 weeks of age, spleens from null mice are 10 fold greater than those of wildtype mice. Neutrophils and bone marrow-derived mast cells from these mice appear to be less susceptible to apoptotic stimuli.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
View Research Applications
Currently there is no phenotype information for this strain.Research Applications
This mouse can be used to support research in many areas including:Inpp5dtm1Dmt related
Cancer Research
Genes Regulating Growth and Proliferation
Cell Biology Research
Genes Regulating Growth and Proliferation
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Intracellular Signaling Molecules
Lymphoid Tissue Defects (myeloid hyperplasia)
Internal/Organ Research
Lymphoid Tissue Defects
Spleen Defects
| Allele Symbol | Inpp5dtm1Dmt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Dan Dumont | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | SHIP-1-; SHIP1-; Ship -; | ||
| Mutation Made By | Dan Dumont, Sunnybrook Health Center | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Inpp5d, inositol polyphosphate-5-phosphatase D | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | AI323613; MGC104855; MGC142140; MGC142142; SHIP; SHIP-1; SHIP1; SIP-145; Src homology 2 domain-containing inositol-5-phosphatase; expressed sequence AI323613; hp51CN; s-SHIP; | ||
| Molecular Note | A neomycin resistance cassette replaced all of exon 1 and part of intron 1. [MGI Ref ID J:72477] | ||
Genotyping Protocols
Inpp5dtm1Dmt, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Liu Q; Sasaki T; Kozieradzki I; Wakeham A; Itie A; Dumont DJ; Penninger JM. 1999. SHIP is a negative regulator of growth factor receptor-mediated PKB/Akt activation and myeloid cell survival. Genes Dev 13(7):786-91. [PubMed: 10197978] [MGI Ref ID J:77739]
Inpp5dtm1Dmt relatedHarder KW; Quilici C; Naik E; Inglese M; Kountouri N; Turner A; Zlatic K; Tarlinton DM; Hibbs ML. 2004. Perturbed myelo/erythropoiesis in Lyn-deficient mice is similar to that in mice lacking the inhibitory phosphatases SHP-1 and SHIP-1. Blood 104(13):3901-10. [PubMed: 15339845] [MGI Ref ID J:95291]
Liu Q; Oliveira-Dos-Santos AJ; Mariathasan S; Bouchard D; Jones J; Sarao R; Kozieradzki I; Ohashi PS; Penninger JM; Dumont DJ. 1998. The inositol polyphosphate 5-phosphatase ship is a crucial negative regulator of B cell antigen receptor signaling. J Exp Med 188(7):1333-42. [PubMed: 9763612] [MGI Ref ID J:72477]
Maxwell MJ; Yuan Y; Anderson KE; Hibbs ML; Salem HH; Jackson SP. 2004. SHIP1 and Lyn Kinase Negatively Regulate Integrin alpha IIb beta 3 signaling in platelets. J Biol Chem 279(31):32196-204. [PubMed: 15166241] [MGI Ref ID J:91755]
Nishio M; Watanabe K; Sasaki J; Taya C; Takasuga S; Iizuka R; Balla T; Yamazaki M; Watanabe H; Itoh R; Kuroda S; Horie Y; Forster I; Mak TW; Yonekawa H; Penninger JM; Kanaho Y; Suzuki A; Sasaki T. 2007. Control of cell polarity and motility by the PtdIns(3,4,5)P3 phosphatase SHIP1. Nat Cell Biol 9(1):36-44. [PubMed: 17173042] [MGI Ref ID J:126426]
Severin S; Gratacap MP; Lenain N; Alvarez L; Hollande E; Penninger JM; Gachet C; Plantavid M; Payrastre B. 2007. Deficiency of Src homology 2 domain-containing inositol 5-phosphatase 1 affects platelet responses and thrombus growth. J Clin Invest 117(4):944-52. [PubMed: 17347685] [MGI Ref ID J:121283]
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
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Contact Information
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Technical Support Email Form
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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