Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse   Donating Investigator Dan Dumont,   Sunnybrook Health Center

Appearance
white-bellied agouti
Related Genotype: A^w/?

black
Related Genotype: a/a

Description
Inpp5d (commonly called SHIP) -deficient mice are viable and fertile. However, fertility rate is 10% lower in homozygotes and lifespan is shortened in comparison to controls. Inpp5d -deficient mice exhibit dramatic chronic hyperplasia of myeloid cells resulting in splenomegaly, lymphadenopathy and myeloid infiltration of vital organs. Histological and flow studies demonstrate a substantial increase in granulocyte-macrophage/monocyte populations in bone marrow, spleen and lymph nodes. Spleens and lymph nodes are severely enlarged compared to wildtype. The difference in spleen size is apparent as early as 4 weeks of age, and by 16 weeks of age, spleens from null mice are 10 fold greater than those of wildtype mice. Neutrophils and bone marrow-derived mast cells from these mice appear to be less susceptible to apoptotic stimuli.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Inpp5d^tm1Dmt related

Cancer Research
Genes Regulating Growth and Proliferation

Cell Biology Research
Genes Regulating Growth and Proliferation

Hematological Research
Hematopoietic Defects

Immunology and Inflammation Research
Intracellular Signaling Molecules
Lymphoid Tissue Defects (myeloid hyperplasia)

Internal/Organ Research
Lymphoid Tissue Defects
Spleen Defects

Currently there is no phenotype information for this strain.

Genes & Alleles

Gene & Allele Information

Allele Symbol		Inpp5dtm1Dmt
Allele Name		targeted mutation 1, Dan Dumont
Allele Type		Targeted (knock-out)
Common Name(s)		SHIP-1-; SHIP1-; Ship -;
Mutation Made By		Dan Dumont,   Sunnybrook Health Center
Strain of Origin		129P2/OlaHsd
ES Cell Line Name		E14
ES Cell Line Strain		129P2/OlaHsd
Gene Symbol and Name		Inpp5d, inositol polyphosphate-5-phosphatase D
Chromosome		1
Gene Common Name(s)		AI323613; MGC104855; MGC142140; MGC142142; SHIP; SHIP-1; SHIP1; SIP-145; Src homology 2 domain-containing inositol-5-phosphatase; expressed sequence AI323613; hp51CN; s-SHIP;
Molecular Note		A neomycin resistance cassette replaced all of exon 1 and part of intron 1. [MGI Ref ID J:72477]

Genotyping

Genotyping Information

Genotyping Protocols

Inpp5d^tm1Dmt, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Liu Q; Sasaki T; Kozieradzki I; Wakeham A; Itie A; Dumont DJ; Penninger JM. 1999. SHIP is a negative regulator of growth factor receptor-mediated PKB/Akt activation and myeloid cell survival. Genes Dev 13(7):786-91. [PubMed: 10197978]  [MGI Ref ID J:77739]

Additional References

Inpp5d^tm1Dmt related

Harder KW; Quilici C; Naik E; Inglese M; Kountouri N; Turner A; Zlatic K; Tarlinton DM; Hibbs ML. 2004. Perturbed myelo/erythropoiesis in Lyn-deficient mice is similar to that in mice lacking the inhibitory phosphatases SHP-1 and SHIP-1. Blood 104(13):3901-10. [PubMed: 15339845]  [MGI Ref ID J:95291]

Liu Q; Oliveira-Dos-Santos AJ; Mariathasan S; Bouchard D; Jones J; Sarao R; Kozieradzki I; Ohashi PS; Penninger JM; Dumont DJ. 1998. The inositol polyphosphate 5-phosphatase ship is a crucial negative regulator of B cell antigen receptor signaling. J Exp Med 188(7):1333-42. [PubMed: 9763612]  [MGI Ref ID J:72477]

Maxwell MJ; Yuan Y; Anderson KE; Hibbs ML; Salem HH; Jackson SP. 2004. SHIP1 and Lyn Kinase Negatively Regulate Integrin alpha IIb beta 3 signaling in platelets. J Biol Chem 279(31):32196-204. [PubMed: 15166241]  [MGI Ref ID J:91755]

Nishio M; Watanabe K; Sasaki J; Taya C; Takasuga S; Iizuka R; Balla T; Yamazaki M; Watanabe H; Itoh R; Kuroda S; Horie Y; Forster I; Mak TW; Yonekawa H; Penninger JM; Kanaho Y; Suzuki A; Sasaki T. 2007. Control of cell polarity and motility by the PtdIns(3,4,5)P3 phosphatase SHIP1. Nat Cell Biol 9(1):36-44. [PubMed: 17173042]  [MGI Ref ID J:126426]

Severin S; Gratacap MP; Lenain N; Alvarez L; Hollande E; Penninger JM; Gachet C; Plantavid M; Payrastre B. 2007. Deficiency of Src homology 2 domain-containing inositol 5-phosphatase 1 affects platelet responses and thrombus growth. J Clin Invest 117(4):944-52. [PubMed: 17347685]  [MGI Ref ID J:121283]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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