Strain Name:

B6.129-Kif3atm1Gsn/J

Stock Number:

003537

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129X1 x 129S1 via R1 (+Kitl-SlJ) ES cell line
 
Donating Investigator Joseph R. Marszalek,   UCSD-HHMI

Description
Ciliary formation appears to be crucial in developing left-right asymmetry in early mouse embryonic development. One of the necessary cilia components is the Kif3a gene product. Homozygous Kif3a knockout mice die at 10 days postcoitum, exhibit randomized establishment of left-right asymmetry and display numerous structural abnormalities. Cardiac looping is randomized, often retarded. Growth is severely retarded with caudal truncation. A neural tube closure defect is also apparent. Scanning electron microscopy indicates the absence of embryonic cilia. A small percentage of heterozygotes exhibit morphological abnormalities.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Kif3atm1Gsn/Kif3a+

        involves: 129S1/Sv * 129X1/SvJ
  • embryogenesis phenotype
  • abnormal embryo turning
    • at E9.5, a subset (4 of 34) heterozygotes fail to undergo turning from lordotic to fetal position   (MGI Ref ID J:54575)
  • decreased embryo size
    • after E9.0, a subset (4 of 34) heterozygotes appear smaller than all wild-type embryos and most heterozygous (30/34) littermates   (MGI Ref ID J:54575)
  • cardiovascular system phenotype
  • abnormal heart looping
    • at E9.5, a subset (4 of 34) heterozygotes display retarded but normal cardiac looping along with pericardial edema   (MGI Ref ID J:54575)
  • pericardial edema
    • at E9.5, a subset (4 of 34) heterozygotes display pericardial edema   (MGI Ref ID J:54575)
  • growth/size/body phenotype
  • decreased embryo size
    • after E9.0, a subset (4 of 34) heterozygotes appear smaller than all wild-type embryos and most heterozygous (30/34) littermates   (MGI Ref ID J:54575)
  • homeostasis/metabolism phenotype
  • pericardial edema
    • at E9.5, a subset (4 of 34) heterozygotes display pericardial edema   (MGI Ref ID J:54575)

Kif3atm1Gsn/Kif3atm1Gsn

        involves: 129S1/Sv * 129X1/SvJ
  • mortality/aging
  • complete embryonic lethality during organogenesis
    • homozygotes die at E10   (MGI Ref ID J:54575)
  • embryogenesis phenotype
  • abnormal embryo turning
    • at E9.5, all homozygotes fail to undergo turning from lordotic to fetal position   (MGI Ref ID J:54575)
  • abnormal left-right axis patterning
    • at E9.5, homozygotes display randomized establishment of L-R asymmetry   (MGI Ref ID J:54575)
  • abnormal neural tube closure
    • at E9.5, all homozygotes display defects in neural tube closure   (MGI Ref ID J:54575)
  • abnormal primitive node morphology
    • at E7.0-E7.5, the mutant embryonic node is normally positioned; however, the nodal plate appears flatter than normal, and overgrowth of surrounding endodermal cells is often observed   (MGI Ref ID J:54575)
    • no other obvious morphological defects are noted prior to E9.0   (MGI Ref ID J:54575)
    • absent embryonic cilia
      • at E7.0-E7.5, homozygotes consistently lack cilia on all cells of the embryonic node   (MGI Ref ID J:54575)
  • branchial arch hypoplasia
    • at E9.5, all homozygotes exhibit reduced branchial arches   (MGI Ref ID J:54575)
  • caudal body truncation
    • at E9.5, all homozygotes are caudally truncated   (MGI Ref ID J:54575)
  • decreased embryo size
    • after E9.0, all homozygous mutant embryos appear smaller than wild-type embryos   (MGI Ref ID J:54575)
  • cardiovascular system phenotype
  • abnormal heart looping
    • at E9.5, 7 of 13 homozygotes exhibit either delayed or reversed heart looping   (MGI Ref ID J:54575)
    • the remaining (6 of 13) homozygotes show normal cardiac looping   (MGI Ref ID J:54575)
    • abnormal direction of heart looping
      • at E9.5, 4 of 13 homozygotes show reversed cardiac looping   (MGI Ref ID J:54575)
    • delayed heart looping
      • at E9.5, 3 of 13 homozygotes display delayed looping accompanied by pericardial edema   (MGI Ref ID J:54575)
  • pericardial edema
    • at E9.5, many homozygotes show a globular pericardium with edema   (MGI Ref ID J:54575)
  • growth/size/body phenotype
  • decreased embryo size
    • after E9.0, all homozygous mutant embryos appear smaller than wild-type embryos   (MGI Ref ID J:54575)
  • situs inversus
    • at E9.5, 4 of 13 homozygotes show reversed cardiac looping   (MGI Ref ID J:54575)
  • limbs/digits/tail phenotype
  • abnormal limb development
    • at E9.5, all homozygotes exhibit reduced or absent limb primordia   (MGI Ref ID J:54575)
  • nervous system phenotype
  • abnormal neural tube closure
    • at E9.5, all homozygotes display defects in neural tube closure   (MGI Ref ID J:54575)
  • craniofacial phenotype
  • branchial arch hypoplasia
    • at E9.5, all homozygotes exhibit reduced branchial arches   (MGI Ref ID J:54575)
  • homeostasis/metabolism phenotype
  • pericardial edema
    • at E9.5, many homozygotes show a globular pericardium with edema   (MGI Ref ID J:54575)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Kif3atm1Gsn related

Cardiovascular Research
Heart Abnormalities

Cell Biology Research
Cell Motility Defects

Developmental Biology Research
Cell Motility Defects
      situs inversus
Growth Defects
Internal/Organ Defects
Neural Tube Defects
Postnatal Lethality
      Homozygous
Skeletal Defects

Internal/Organ Research
Heart Abnormalities

Neurobiology Research
Neural Tube Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Kif3atm1Gsn
Allele Name targeted mutation 1, Lawrence SB Goldstein
Allele Type Targeted (knock-out)
Common Name(s) KIF3A-; Kif3an; Kif3atm1Maz;
Mutation Made By Joseph Marszalek,   UCSD-HHMI
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Kif3a, kinesin family member 3A
Chromosome 11
Gene Common Name(s) AF180004; AF180009; AW124694; DNA sequence AF180004; DNA sequence AF180009; FLA10; KLP-20; Kif3; Kifl; Kns3; N-4 kinesin; expressed sequence AW124694; kinesin family member 3; kinesin family-like; kinesin-II subunit;
Molecular Note Exon 2 was deleted by cre recombinase removal of a targeting vector containing the exon flanked by loxP sites. The resulting transcript was translated out of reading frame, generating a 62 amino acid nonsense protein. Northern and Western blots of heterozygous mutants showed that no fragments or aberrant products were made from the mutant gene. Western blots of homozygous mutants showed an absence of the targeted protein. This allele was generated from Kif3atm2Gsn. [MGI Ref ID J:54575]

Genotyping

Genotyping Information

Genotyping Protocols

Kif3atm1Gsn, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Marszalek JR; Ruiz-Lozano P; Roberts E; Chien KR; Goldstein LS. 1999. Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc Natl Acad Sci U S A 96(9):5043-8. [PubMed: 10220415]  [MGI Ref ID J:54575]

Additional References

Kif3atm1Gsn related

Botilde Y; Yoshiba S; Shinohara K; Hasegawa T; Nishimura H; Shiratori H; Hamada H. 2013. Cluap1 localizes preferentially to the base and tip of cilia and is required for ciliogenesis in the mouse embryo. Dev Biol 381(1):203-12. [PubMed: 23742838]  [MGI Ref ID J:200709]

Cho A; Ko HW; Eggenschwiler JT. 2008. FKBP8 cell-autonomously controls neural tube patterning through a Gli2- and Kif3a-dependent mechanism. Dev Biol 321(1):27-39. [PubMed: 18590716]  [MGI Ref ID J:138713]

Haycraft CJ; Zhang Q; Song B; Jackson WS; Detloff PJ; Serra R; Yoder BK. 2007. Intraflagellar transport is essential for endochondral bone formation. Development 134(2):307-16. [PubMed: 17166921]  [MGI Ref ID J:117033]

Huangfu D; Anderson KV. 2005. Cilia and Hedgehog responsiveness in the mouse. Proc Natl Acad Sci U S A 102(32):11325-30. [PubMed: 16061793]  [MGI Ref ID J:100466]

Huangfu D; Liu A; Rakeman AS; Murcia NS; Niswander L; Anderson KV. 2003. Hedgehog signalling in the mouse requires intraflagellar transport proteins. Nature 426(6962):83-7. [PubMed: 14603322]  [MGI Ref ID J:86437]

Lancaster MA; Schroth J; Gleeson JG. 2011. Subcellular spatial regulation of canonical Wnt signalling at the primary cilium. Nat Cell Biol 13(6):700-7. [PubMed: 21602792]  [MGI Ref ID J:174986]

Lin F; Hiesberger T; Cordes K; Sinclair AM; Goldstein LS; Somlo S; Igarashi P. 2003. Kidney-specific inactivation of the KIF3A subunit of kinesin-II inhibits renal ciliogenesis and produces polycystic kidney disease. Proc Natl Acad Sci U S A 100(9):5286-91. [PubMed: 12672950]  [MGI Ref ID J:83293]

Mukhopadhyay S; Wen X; Ratti N; Loktev A; Rangell L; Scales SJ; Jackson PK. 2013. The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling. Cell 152(1-2):210-23. [PubMed: 23332756]  [MGI Ref ID J:193395]

Norman RX; Ko HW; Huang V; Eun CM; Abler LL; Zhang Z; Sun X; Eggenschwiler JT. 2009. Tubby-like protein 3 (TULP3) regulates patterning in the mouse embryo through inhibition of Hedgehog signaling. Hum Mol Genet 18(10):1740-54. [PubMed: 19286674]  [MGI Ref ID J:147574]

Paez-Gonzalez P; Abdi K; Luciano D; Liu Y; Soriano-Navarro M; Rawlins E; Bennett V; Garcia-Verdugo JM; Kuo CT. 2011. Ank3-Dependent SVZ Niche Assembly Is Required for the Continued Production of New Neurons. Neuron 71(1):61-75. [PubMed: 21745638]  [MGI Ref ID J:174694]

Patel V; Li L; Cobo-Stark P; Shao X; Somlo S; Lin F; Igarashi P. 2008. Acute kidney injury and aberrant planar cell polarity induce cyst formation in mice lacking renal cilia. Hum Mol Genet 17(11):1578-90. [PubMed: 18263895]  [MGI Ref ID J:135523]

Qin J; Lin Y; Norman RX; Ko HW; Eggenschwiler JT. 2011. Intraflagellar transport protein 122 antagonizes Sonic Hedgehog signaling and controls ciliary localization of pathway components. Proc Natl Acad Sci U S A 108(4):1456-61. [PubMed: 21209331]  [MGI Ref ID J:168317]

Qiu N; Cao L; David V; Quarles LD; Xiao Z. 2010. Kif3a deficiency reverses the skeletal abnormalities in Pkd1 deficient mice by restoring the balance between osteogenesis and adipogenesis. PLoS One 5(12):e15240. [PubMed: 21151991]  [MGI Ref ID J:167728]

Qiu N; Xiao Z; Cao L; Buechel MM; David V; Roan E; Quarles LD. 2012. Disruption of Kif3a in osteoblasts results in defective bone formation and osteopenia. J Cell Sci 125(Pt 8):1945-57. [PubMed: 22357948]  [MGI Ref ID J:197714]

Raya A; Kawakami Y; Rodriguez-Esteban C; Buscher D; Koth CM; Itoh T; Morita M; Raya RM; Dubova I; Bessa JG; de la Pompa JL; Belmonte JC. 2003. Notch activity induces Nodal expression and mediates the establishment of left-right asymmetry in vertebrate embryos. Genes Dev 17(10):1213-8. [PubMed: 12730123]  [MGI Ref ID J:83387]

Spassky N; Han YG; Aguilar A; Strehl L; Besse L; Laclef C; Ros MR; Garcia-Verdugo JM; Alvarez-Buylla A. 2008. Primary cilia are required for cerebellar development and Shh-dependent expansion of progenitor pool. Dev Biol 317(1):246-59. [PubMed: 18353302]  [MGI Ref ID J:136079]

Wong SY; Seol AD; So PL; Ermilov AN; Bichakjian CK; Epstein EH Jr; Dlugosz AA; Reiter JF. 2009. Primary cilia can both mediate and suppress Hedgehog pathway-dependent tumorigenesis. Nat Med 15(9):1055-61. [PubMed: 19701205]  [MGI Ref ID J:154128]

Yoshiba S; Shiratori H; Kuo IY; Kawasumi A; Shinohara K; Nonaka S; Asai Y; Sasaki G; Belo JA; Sasaki H; Nakai J; Dworniczak B; Ehrlich BE; Pennekamp P; Hamada H. 2012. Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2. Science 338(6104):226-31. [PubMed: 22983710]  [MGI Ref ID J:187958]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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