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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S4 via J1 ES cell line Donating Investigator Dr. Rudolf Jaenisch, Whitehead Institute (MIT) Description
This strain has loxP sites located on either side of the Ntf3 exon 2. Mice homozygous for this allele are viable, fertile and without behavioral abnormalities. If this strain is crossed with a transgenic strain bearing Cre recombinase, expression of the Ntf3 gene in offspring can be blocked in a tissue-specific manner depending on the promoter controlling the recombinase. Strains under the control of the rat nestin promoter block expression in the central nervous system; see strains 002275 and 002276.When bred to a strain expressing Cre recombinase in skeletal muscle (see Stock No. 007893 for example), this mutant mouse strain may be useful in studies of muscle spindle development
Development
This strain possesses loxP sites introduced on either side of the Ntf3 exon 2.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ntf3
002275 B6.129S4-Ntf3tm1Jae/J 002276 STOCK Ntf3tm1Jae/J View Strains carrying other alleles of Ntf3 (2 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ntf3tm2Jae/Ntf3tm2Jae
Background Not Specified
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:52951)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Myf5tm3(cre)Sor/Myf5+ Ntf3tm2Jae/Ntf3tm2Jae
involves: 129S1/Sv * C57BL/6 (conditional)
- behavior/neurological phenotype
- abnormal gait
- mice exhibit a wide-based, ataxic gait, similar to Erbb2 conditional mutants; onset of abnormalities is delayed and is less pronounced, however, relative to Erbb2 conditional animals (MGI Ref ID J:147957)
- abnormal posture
- mice show a flexed posture when inverted, similar to Erbb2 and Ntf3;Egr3 conditional mutants (MGI Ref ID J:147957)
- ataxia (MGI Ref ID J:147957)
- nervous system phenotype
- abnormal nervous system electrophysiology
- at P5, ventral root potentials are normal, but by P14, amplitudes of potentials are reduced by >75% compared to controls, similar to Ntf3;Egr3 conditional mutants (MGI Ref ID J:147957)
This mouse can be used to support research in many areas including:
Ntf3tm2Jae relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
Apoptosis Research
Extracellular Modulators
Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Developmental Biology Research
Mesodermal Defects
Myogenesis Defects
Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation
Neurobiology Research
Ataxia (Movement) Defects
Neurotrophic Factor Defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ntf3tm2Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rudolf Jaenisch | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Nt-32lox; Ntf3lox; | ||
| Mutation Made By | Dr. Rudolf Jaenisch, Whitehead Institute (MIT) | ||
| Gene Symbol and Name | Ntf3, neurotrophin 3 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AI316846; AI835689; EST AI316846; HDNF; NGF-2; NGF2; NT-3; NT3; Ntf-3; expressed sequence AI835689; | ||
| Molecular Note | Introduction of loxP sites flanking exon 2 of the Ntf3 gene. [MGI Ref ID J:52951] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ntf3tm2Jae, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Bates B; Rios M; Trumpp A; Chen C; Fan G; Bishop JM; Jaenisch R. 1999. Neurotrophin-3 is required for proper cerebellar development. Nat Neurosci 2(2):115-7. [PubMed: 10195193] [MGI Ref ID J:52951]
Additional References
Ntf3tm2Jae relatedAkbarian S; Bates B; Liu RJ; Skirboll SL; Pejchal T; Coppola V; Sun LD; Fan G; Kucera J; Wilson MA; Tessarollo L; Kosofsky BE; Taylor JR; Bothwell M; Nestler EJ; Aghajanian GK; Jaenisch R. 2001. Neurotrophin-3 modulates noradrenergic neuron function and opiate withdrawal. Mol Psychiatry 6(5):593-604. [PubMed: 11526474] [MGI Ref ID J:103960]
Bates B; Hirt L; Thomas SS; Akbarian S; Le D; Amin-Hanjani S; Whalen M; Jaenisch R; Moskowitz MA. 2002. Neurotrophin-3 promotes cell death induced in cerebral ischemia, oxygen-glucose deprivation, and oxidative stress: possible involvement of oxygen free radicals. Neurobiol Dis 9(1):24-37. [PubMed: 11848682] [MGI Ref ID J:75154]
Baydyuk M; Xie Y; Tessarollo L; Xu B. 2013. Midbrain-derived neurotrophins support survival of immature striatal projection neurons. J Neurosci 33(8):3363-9. [PubMed: 23426664] [MGI Ref ID J:194255]
Shalizi A; Lehtinen M; Gaudilliere B; Donovan N; Han J; Konishi Y; Bonni A. 2003. Characterization of a neurotrophin signaling mechanism that mediates neuron survival in a temporally specific pattern. J Neurosci 23(19):7326-36. [PubMed: 12917366] [MGI Ref ID J:120211]
Shimazu K; Zhao M; Sakata K; Akbarian S; Bates B; Jaenisch R; Lu B. 2006. NT-3 facilitates hippocampal plasticity and learning and memory by regulating neurogenesis. Learn Mem 13(3):307-15. [PubMed: 16705139] [MGI Ref ID J:135724]
Shneider NA; Mentis GZ; Schustak J; O'Donovan MJ. 2009. Functionally reduced sensorimotor connections form with normal specificity despite abnormal muscle spindle development: the role of spindle-derived neurotrophin 3. J Neurosci 29(15):4719-35. [PubMed: 19369542] [MGI Ref ID J:147957]
Usui N; Watanabe K; Ono K; Tomita K; Tamamaki N; Ikenaka K; Takebayashi H. 2012. Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation. Development 139(6):1125-32. [PubMed: 22318233] [MGI Ref ID J:182750]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry Homozygotes are viable and fertile. The investigator maintains this strain as a heterozygote on a C57BL/6 background. Coat color expected from breeding:Black Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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