Strain Name:

B6.129S4-Ntf3tm2Jae/J

Stock Number:

003541

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S4 via J1 ES cell line
 
Donating InvestigatorDr. Rudolf Jaenisch,   Whitehead Institute (MIT)

Description
This strain has loxP sites located on either side of the Ntf3 exon 2. Mice homozygous for this allele are viable, fertile and without behavioral abnormalities. If this strain is crossed with a transgenic strain bearing Cre recombinase, expression of the Ntf3 gene in offspring can be blocked in a tissue-specific manner depending on the promoter controlling the recombinase. Strains under the control of the rat nestin promoter block expression in the central nervous system; see strains 002275 and 002276.

When bred to a strain expressing Cre recombinase in skeletal muscle (see Stock No. 007893 for example), this mutant mouse strain may be useful in studies of muscle spindle development

Development
This strain possesses loxP sites introduced on either side of the Ntf3 exon 2.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Ntf3
002275   B6.129S4-Ntf3tm1Jae/J
002276   STOCK Ntf3tm1Jae/J
View Strains carrying other alleles of Ntf3     (2 strains)

Additional Web Information

Introduction to Cre-lox technology

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Ntf3tm2Jae/Ntf3tm2Jae

        Background Not Specified
  • normal phenotype
  • no abnormal phenotype detected   (MGI Ref ID J:52951)

The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.

Myf5tm3(cre)Sor/Myf5+ Ntf3tm2Jae/Ntf3tm2Jae

        involves: 129S1/Sv * C57BL/6   (conditional)
  • behavior/neurological phenotype
  • abnormal gait
    • mice exhibit a wide-based, ataxic gait, similar to Erbb2 conditional mutants; onset of abnormalities is delayed and is less pronounced, however, relative to Erbb2 conditional animals   (MGI Ref ID J:147957)
  • abnormal posture
    • mice show a flexed posture when inverted, similar to Erbb2 and Ntf3;Egr3 conditional mutants   (MGI Ref ID J:147957)
  • ataxia   (MGI Ref ID J:147957)
  • nervous system phenotype
  • abnormal nervous system electrophysiology
    • at P5, ventral root potentials are normal, but by P14, amplitudes of potentials are reduced by >75% compared to controls, similar to Ntf3;Egr3 conditional mutants   (MGI Ref ID J:147957)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      loxP-flanked Sequences

Ntf3tm2Jae related

Apoptosis Research
Extracellular Modulators

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Developmental Biology Research
Mesodermal Defects
      Myogenesis Defects

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation

Neurobiology Research
Ataxia (Movement) Defects
Neurotrophic Factor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ntf3tm2Jae
Allele Name targeted mutation 2, Rudolf Jaenisch
Allele Type Targeted (Floxed/Frt)
Common Name(s) NT3flx; Nt-32lox; Ntf3lox;
Mutation Made ByDr. Rudolf Jaenisch,   Whitehead Institute (MIT)
Gene Symbol and Name Ntf3, neurotrophin 3
Chromosome 6
Gene Common Name(s) AI316846; AI835689; EST AI316846; HDNF; NGF-2; NGF2; NT-3; NT3; Ntf-3; expressed sequence AI835689;
Molecular Note Introduction of loxP sites flanking exon 2 of the Ntf3 gene. [MGI Ref ID J:52951]

Genotyping

Genotyping Information

Genotyping Protocols

Ntf3tm2Jae, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bates B; Rios M; Trumpp A; Chen C; Fan G; Bishop JM; Jaenisch R. 1999. Neurotrophin-3 is required for proper cerebellar development. Nat Neurosci 2(2):115-7. [PubMed: 10195193]  [MGI Ref ID J:52951]

Additional References

Ntf3tm2Jae related

Akbarian S; Bates B; Liu RJ; Skirboll SL; Pejchal T; Coppola V; Sun LD; Fan G; Kucera J; Wilson MA; Tessarollo L; Kosofsky BE; Taylor JR; Bothwell M; Nestler EJ; Aghajanian GK; Jaenisch R. 2001. Neurotrophin-3 modulates noradrenergic neuron function and opiate withdrawal. Mol Psychiatry 6(5):593-604. [PubMed: 11526474]  [MGI Ref ID J:103960]

Bates B; Hirt L; Thomas SS; Akbarian S; Le D; Amin-Hanjani S; Whalen M; Jaenisch R; Moskowitz MA. 2002. Neurotrophin-3 promotes cell death induced in cerebral ischemia, oxygen-glucose deprivation, and oxidative stress: possible involvement of oxygen free radicals. Neurobiol Dis 9(1):24-37. [PubMed: 11848682]  [MGI Ref ID J:75154]

Baydyuk M; Xie Y; Tessarollo L; Xu B. 2013. Midbrain-derived neurotrophins support survival of immature striatal projection neurons. J Neurosci 33(8):3363-9. [PubMed: 23426664]  [MGI Ref ID J:194255]

Fox EA; Biddinger JE; Baquet ZC; Jones KR; McAdams J. 2013. Loss of Neurotrophin-3 from Smooth Muscle Disrupts Vagal Gastrointestinal Afferent Signaling and Satiation. Am J Physiol Regul Integr Comp Physiol :. [PubMed: 24068045]  [MGI Ref ID J:201812]

Shalizi A; Lehtinen M; Gaudilliere B; Donovan N; Han J; Konishi Y; Bonni A. 2003. Characterization of a neurotrophin signaling mechanism that mediates neuron survival in a temporally specific pattern. J Neurosci 23(19):7326-36. [PubMed: 12917366]  [MGI Ref ID J:120211]

Shimazu K; Zhao M; Sakata K; Akbarian S; Bates B; Jaenisch R; Lu B. 2006. NT-3 facilitates hippocampal plasticity and learning and memory by regulating neurogenesis. Learn Mem 13(3):307-15. [PubMed: 16705139]  [MGI Ref ID J:135724]

Shneider NA; Mentis GZ; Schustak J; O'Donovan MJ. 2009. Functionally reduced sensorimotor connections form with normal specificity despite abnormal muscle spindle development: the role of spindle-derived neurotrophin 3. J Neurosci 29(15):4719-35. [PubMed: 19369542]  [MGI Ref ID J:147957]

Usui N; Watanabe K; Ono K; Tomita K; Tamamaki N; Ikenaka K; Takebayashi H. 2012. Role of motoneuron-derived neurotrophin 3 in survival and axonal projection of sensory neurons during neural circuit formation. Development 139(6):1125-32. [PubMed: 22318233]  [MGI Ref ID J:182750]

de Nooij JC; Doobar S; Jessell TM. 2013. Etv1 inactivation reveals proprioceptor subclasses that reflect the level of NT3 expression in muscle targets. Neuron 77(6):1055-68. [PubMed: 23522042]  [MGI Ref ID J:197913]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHomozygotes are viable and fertile. The investigator maintains this strain as a heterozygote on a C57BL/6 background. Coat color expected from breeding:Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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