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Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S4 via J1 ES cell line Generation N13+2pN1pN1 Donating Investigator Rudolf Jaenisch, Massachusetts Institute of Technology Description
This strain has loxP sites located on either side of the Ntf3 exon 2. Mice homozygous for this allele are viable, fertile and without behavioral abnormalities. If this strain is crossed with a transgenic strain bearing Cre recombinase, expression of the Ntf3 gene in offspring can be blocked in a tissue-specific manner depending on the promoter controlling the recombinase. Strains under the control of the rat nestin promoter block expression in the central nervous system; see strains 002275 and 002276.Development
This strain possesses loxP sites introduced on either side of the Ntf3 exon 2.
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Ntf3
002275 B6.129S4-Ntf3tm1Jae/J 002276 STOCK Ntf3tm1Jae/J View Strains carrying other alleles of Ntf3 (2 strains)
Congenic Nomenclature
Cre-lox Systems
View Mammalian Phenotype Terms
Mammalian Phenotype Terms
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ntf3tm2Jae/Ntf3tm2Jae
Background Not Specified
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:52951)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
Ntf3tm2Jae relatedResearch Tools
Cre-lox System (loxP-flanked Sequences)
Apoptosis Research
Extracellular Modulators
Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines
Developmental Biology Research
Mesodermal Defects (Myogenesis Defects)
Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Inflammation
Neurobiology Research
Ataxia (Movement) Defects
Neurotrophic Factor Defects
Vestibular and Hearing Defects
Sensorineural Research
Vestibular and Hearing Defects
| Allele Symbol | Ntf3tm2Jae | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Rudolf Jaenisch | ||
| Allele Type | Targeted (Floxed/Frt) | ||
| Common Name(s) | Nt-32lox; | ||
| Mutation Made By | Rudolf Jaenisch, Massachusetts Institute of Technology | ||
| Gene Symbol and Name | Ntf3, neurotrophin 3 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AI316846; AI835689; EST AI316846; HDNF; MGC129711; NGF-2; NGF2; NT-3; NT3; Ntf-3; expressed sequence AI835689; | ||
| Molecular Note | Introduction of loxP sites flanking exon 2 of the Ntf3 gene. [MGI Ref ID J:52951] | ||
Genotyping Protocols
Ntf3tm2Jae, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
Bates B; Rios M; Trumpp A; Chen C; Fan G; Bishop JM; Jaenisch R. 1999. Neurotrophin-3 is required for proper cerebellar development. Nat Neurosci 2(2):115-7. [PubMed: 10195193] [MGI Ref ID J:52951]
Ntf3tm2Jae relatedAkbarian S; Bates B; Liu RJ; Skirboll SL; Pejchal T; Coppola V; Sun LD; Fan G; Kucera J; Wilson MA; Tessarollo L; Kosofsky BE; Taylor JR; Bothwell M; Nestler EJ; Aghajanian GK; Jaenisch R. 2001. Neurotrophin-3 modulates noradrenergic neuron function and opiate withdrawal. Mol Psychiatry 6(5):593-604. [PubMed: 11526474] [MGI Ref ID J:103960]
Bates B; Hirt L; Thomas SS; Akbarian S; Le D; Amin-Hanjani S; Whalen M; Jaenisch R; Moskowitz MA. 2002. Neurotrophin-3 promotes cell death induced in cerebral ischemia, oxygen-glucose deprivation, and oxidative stress: possible involvement of oxygen free radicals. Neurobiol Dis 9(1):24-37. [PubMed: 11848682] [MGI Ref ID J:75154]
Shalizi A; Lehtinen M; Gaudilliere B; Donovan N; Han J; Konishi Y; Bonni A. 2003. Characterization of a neurotrophin signaling mechanism that mediates neuron survival in a temporally specific pattern. J Neurosci 23(19):7326-36. [PubMed: 12917366] [MGI Ref ID J:120211]
Shimazu K; Zhao M; Sakata K; Akbarian S; Bates B; Jaenisch R; Lu B. 2006. NT-3 facilitates hippocampal plasticity and learning and memory by regulating neurogenesis. Learn Mem 13(3):307-15. [PubMed: 16705139] [MGI Ref ID J:135724]
Colony Maintenance
Breeding & Husbandry Homozygotes are viable and fertile. The investigator maintains this strain as a heterozygote on a C57BL/6 background. Coat color expected from breeding:Black Diet Information LabDiet® 5K52/5K67
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
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Contact Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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