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Description
Strain Information
Former Names B6129-Tg(MMTV-cre)1Mam/J (Changed: 15-DEC-04 ) B6129-TgN(MMTV-Cre)1Mam (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation +N1 Donating Investigator Lothar Hennighausen, National Institutes of Health Description
This transgenic strain expresses P1 Cre recombinase under the control of the MMTV LTR promoter. The MMTV LTR promoter directs a widespread pattern of expression including the female germline. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, and various cells of the immune system. Little background recombination was observed in the lung, kidney, liver and brain tissues. The donating investigator indicates that this strain may be suitable for use in applications where it is desirable to delete genes in the virgin and lactating mammary gland, and other secretory tissues. Furthermore, since this strain expresses cre in the female germline, it can also be utilized to generate a knockout allele from a targeted locus with loxP sites with an efficiency of 100%.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (126 strains)
004997 B6.Cg-Tg(MMTV-TGFBR2)7Hlm/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002375 B6;D2-Tg(MMTVTGFB1)46Hlm/J 002459 B6D2-Tg(MMTVTGFA)254Rjc/J 002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 004363 FVB.Cg-Tg(MMTV-vHaras)SH1Led/J 002953 FVB.Cg-Tg(MMTVTGFA)254Rjc/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J 002374 FVB/N-Tg(MMTV-PyVT)634Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J 002933 FVB/NJ-Tg(MMTVTGFB1)46Hlm/J 003690 STOCK Tg(MMTV-Cdc37)1Stp/J 003337 STOCK Tg(MMTV-PIP)1Shu/J 003553 STOCK Tg(MMTV-cre)4Mam/J
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(MMTV-cre)1Mam | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Lothar Hennighausen | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | A-line; CreA; F-line; MMTV-Cre; MMTV-CreA; | ||
| Mutation Made By | Kay-Uwe Wagner, University of Nebraska Medical Center | ||
| Strain of Origin | FVB | ||
| Site of Expression | widespread pattern of expression including the female germline; high levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, and various cells of the immune system; little background recombination was observed in the lung, kidney, liver and brain tissues | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV | ||
| General Note | This transgene is the A line. Line 4 (D) was also produced. | ||
| Molecular Note | This transgene directs the expression of Cre recombinase under the control of the mouse mammary tumor virus (MMTV) long terminal repeat. The viral MMTV promoter directed expression of Cre recombinase in the secretory epithelium of the mammary gland, thesalivary gland and in the female germline. High levels of recombination have been detected in the virgin and lactating mammary gland, salivary gland, seminal vesicle, skin, and various cells of the immune system. Little background recombination was observed in the lung, kidney, liver and brain tissues. [MGI Ref ID J:67925] [MGI Ref ID J:74366] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(MMTV-cre)1Mam, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Wagner KU; Wall RJ; St-Onge L; Gruss P; Wynshaw-Boris A; Garrett L; Li M; Furth PA; Hennighausen L. 1997. Cre-mediated gene deletion in the mammary gland. Nucleic Acids Res 25(21):4323-30. [PubMed: 9336464] [MGI Ref ID J:67925]
Additional References
Miki K; Willis WD; Brown PR; Goulding EH; Fulcher KD; Eddy EM. 2002. Targeted disruption of the Akap4 gene causes defects in sperm flagellum and motility. Dev Biol 248(2):331-42. [PubMed: 12167408] [MGI Ref ID J:86899]
Wagner KU; Krempler A; Qi Y; Park K; Henry MD; Triplett AA; Riedlinger G; Rucker III EB; Hennighausen L. 2003. Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissues. Mol Cell Biol 23(1):150-62. [PubMed: 12482969] [MGI Ref ID J:80891]
Tg(MMTV-cre)1Mam relatedBierie B; Nozawa M; Renou JP; Shillingford JM; Morgan F; Oka T; Taketo MM; Cardiff RD; Miyoshi K; Wagner KU; Robinson GW; Hennighausen L. 2003. Activation of beta-catenin in prostate epithelium induces hyperplasias and squamous transdifferentiation. Oncogene 22(25):3875-87. [PubMed: 12813461] [MGI Ref ID J:84340]
Bowen TJ; Yakushiji H; Montagna C; Jain S; Ried T; Wynshaw-Boris A. 2005. Atm heterozygosity cooperates with loss of Brca1 to increase the severity of mammary gland cancer and reduce ductal branching. Cancer Res 65(19):8736-46. [PubMed: 16204043] [MGI Ref ID J:102687]
Buono KD; Robinson GW; Martin C; Shi S; Stanley P; Tanigaki K; Honjo T; Hennighausen L. 2006. The canonical Notch/RBP-J signaling pathway controls the balance of cell lineages in mammary epithelium during pregnancy. Dev Biol 293(2):565-80. [PubMed: 16581056] [MGI Ref ID J:108712]
Cheung AM; Elia A; Tsao MS; Done S; Wagner KU; Hennighausen L; Hakem R; Mak TW. 2004. Brca2 deficiency does not impair mammary epithelium development but promotes mammary adenocarcinoma formation in p53(+/-) mutant mice. Cancer Res 64(6):1959-65. [PubMed: 15026330] [MGI Ref ID J:89150]
Cui Y; Miyoshi K; Claudio E; Siebenlist UK; Gonzalez FJ; Flaws J; Wagner KU; Hennighausen L. 2002. Loss of the peroxisome proliferation-activated receptor gamma (PPARgamma ) does not affect mammary development and propensity for tumor formation but leads to reduced fertility. J Biol Chem 277(20):17830-5. [PubMed: 11884400] [MGI Ref ID J:76553]
Davis MA; Reynolds AB. 2006. Blocked acinar development, E-cadherin reduction, and intraepithelial neoplasia upon ablation of p120-catenin in the mouse salivary gland. Dev Cell 10(1):21-31. [PubMed: 16399075] [MGI Ref ID J:105283]
Declercq J; Skaland I; Van Dyck F; Janssen EA; Baak JP; Drijkoningen M; Van de Ven WJ. 2008. Adenomyoepitheliomatous lesions of the mammary glands in transgenic mice with targeted PLAG1 overexpression. Int J Cancer 123(7):1593-600. [PubMed: 18649356] [MGI Ref ID J:140057]
Declercq J; Van Dyck F; Braem CV; Van Valckenborgh IC; Voz M; Wassef M; Schoonjans L; Van Damme B; Fiette L; Van de Ven WJ. 2005. Salivary gland tumors in transgenic mice with targeted PLAG1 proto-oncogene overexpression. Cancer Res 65(11):4544-53. [PubMed: 15930271] [MGI Ref ID J:98825]
Kim BG; Li C; Qiao W; Mamura M; Kasperczak B; Anver M; Wolfraim L; Hong S; Mushinski E; Potter M; Kim SJ; Fu XY; Deng C; Letterio JJ. 2006. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Nature 441(7096):1015-9. [PubMed: 16791201] [MGI Ref ID J:110041]
Krempler A; Qi Y; Triplett AA; Zhu J; Rui H; Wagner KU. 2004. Generation of a conditional knockout allele for the Janus kinase 2 (Jak2) gene in mice. Genesis 40(1):52-7. [PubMed: 15354294] [MGI Ref ID J:92299]
Le Provost F; Riedlinger G; Hee Yim S; Benedict J; Gonzalez FJ; Flaws J; Hennighausen L. 2002. The aryl hydrocarbon receptor (AhR) and its nuclear translocator (Arnt) are dispensable for normal mammary gland development but are required for fertility. Genesis 32(3):231-9. [PubMed: 11892012] [MGI Ref ID J:76280]
Li G; Robinson GW; Lesche R; Martinez-Diaz H; Jiang Z; Rozengurt N; Wagner KU; Wu DC; Lane TF; Liu X; Hennighausen L; Wu H. 2002. Conditional loss of PTEN leads to precocious development and neoplasia in the mammary gland. Development 129(17):4159-70. [PubMed: 12163417] [MGI Ref ID J:78415]
Li W; Qiao W; Chen L; Xu X; Yang X; Li D; Li C; Brodie SG; Meguid MM; Hennighausen L; Deng CX. 2003. Squamous cell carcinoma and mammary abscess formation through squamous metaplasia in Smad4/Dpc4 conditional knockout mice. Development 130(24):6143-53. [PubMed: 14597578] [MGI Ref ID J:86484]
Li Z; Tognon CE; Godinho FJ; Yasaitis L; Hock H; Herschkowitz JI; Lannon CL; Cho E; Kim SJ; Bronson RT; Perou CM; Sorensen PH; Orkin SH. 2007. ETV6-NTRK3 fusion oncogene initiates breast cancer from committed mammary progenitors via activation of AP1 complex. Cancer Cell 12(6):542-58. [PubMed: 18068631] [MGI Ref ID J:130323]
Liao D; Corle C; Seagroves TN; Johnson RS. 2007. Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Cancer Res 67(2):563-72. [PubMed: 17234764] [MGI Ref ID J:117422]
Loladze AV; Stull MA; Rowzee AM; Demarco J; Lantry JH rd; Rosen CJ; Leroith D; Wagner KU; Hennighausen L; Wood TL. 2006. Epithelial-specific and stage-specific functions of insulin-like growth factor-I during postnatal mammary development. Endocrinology 147(11):5412-23. [PubMed: 16901968] [MGI Ref ID J:117180]
Mohrs M; Blankespoor CM; Wang ZE; Loots GG; Afzal V; Hadeiba H; Shinkai K; Rubin EM; Locksley RM. 2001. Deletion of a coordinate regulator of type 2 cytokine expression in mice. Nat Immunol 2(9):842-7. [PubMed: 11526400] [MGI Ref ID J:125684]
Nagy T; Wei H; Shen TL; Peng X; Liang CC; Gan B; Guan JL. 2007. Mammary epithelial-specific deletion of the focal adhesion kinase gene leads to severe lobulo-alveolar hypoplasia and secretory immaturity of the murine mammary gland. J Biol Chem 282(43):31766-76. [PubMed: 17716968] [MGI Ref ID J:126750]
Odet F; Duan C; Willis W; Goulding E; Kung A; Eddy E; Goldberg E. 2008. Expression of the gene for Lactate Dehydrogenase C: Ldhc is required for male fertility Biol Reprod 79(1):26-34. [PubMed: 18367675] [MGI Ref ID J:132072]
Qi C; Kashireddy P; Zhu YT; Rao SM; Zhu YJ. 2004. Null mutation of peroxisome proliferator-activated receptor-interacting protein in mammary glands causes defective mammopoiesis. J Biol Chem 279(32):33696-701. [PubMed: 15161927] [MGI Ref ID J:121207]
Riedlinger G; Okagaki R; Wagner KU; Rucker EB rd; Oka T; Miyoshi K; Flaws JA; Hennighausen L. 2002. Bcl-x is not required for maintenance of follicles and corpus luteum in the postnatal mouse ovary. Biol Reprod 66(2):438-44. [PubMed: 11804960] [MGI Ref ID J:108582]
Robinson GW; Pacher-Zavisin M; Zhu BM; Yoshimura A; Hennighausen L. 2007. Socs 3 modulates the activity of the transcription factor Stat3 in mammary tissue and controls alveolar homeostasis. Dev Dyn 236(3):654-61. [PubMed: 17205581] [MGI Ref ID J:118349]
Seagroves TN; Hadsell D; McManaman J; Palmer C; Liao D; McNulty W; Welm B; Wagner KU; Neville M; Johnson RS. 2003. HIF1alpha is a critical regulator of secretory differentiation and activation, but not vascular expansion, in the mouse mammary gland. Development 130(8):1713-24. [PubMed: 12620994] [MGI Ref ID J:82618]
Wagner KU; Claudio E; Rucker EB rd; Riedlinger G; Broussard C; Schwartzberg PL; Siebenlist U; Hennighausen L. 2000. Conditional deletion of the Bcl-x gene from erythroid cells results in hemolytic anemia and profound splenomegaly. Development 127(22):4949-58. [PubMed: 11044408] [MGI Ref ID J:75416]
Wagner KU; Krempler A; Qi Y; Park K; Henry MD; Triplett AA; Riedlinger G; Rucker III EB; Hennighausen L. 2003. Tsg101 is essential for cell growth, proliferation, and cell survival of embryonic and adult tissues. Mol Cell Biol 23(1):150-62. [PubMed: 12482969] [MGI Ref ID J:80891]
Wagner KU; Krempler A; Triplett AA; Qi Y; George NM; Zhu J; Rui H. 2004. Impaired alveologenesis and maintenance of secretory mammary epithelial cells in Jak2 conditional knockout mice. Mol Cell Biol 24(12):5510-20. [PubMed: 15169911] [MGI Ref ID J:90883]
Wagner KU; McAllister K; Ward T; Davis B; Wiseman R; Hennighausen L. 2001. Spatial and temporal expression of the Cre gene under the control of the MMTV-LTR in different lines of transgenic mice. Transgenic Res 10(6):545-53. [PubMed: 11817542] [MGI Ref ID J:74366]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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