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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;J-Tg(Col2a1-cre)1Bhr/J (Changed: 15-DEC-04 ) B6;SJL-TgN(Col2a1-Cre)1Bhr (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System +/+ sibling x Hemizygote (Female x Male) 01-MAR-11 Species laboratory mouse Donating Investigator Dr. Richard Behringer, Univ of Texas, MD Anderson Cancer Center Description
This strain expresses Cre recombinase in a chondrocyte-specific pattern under the control of a Col2a1 promoter.Development
This strain was generated on a B6SJLF1 background. The founder was crossed to C57BL/6 twice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Col2a1
003916 B6(Cg)-Col2a1sedc/GrsrJ 012724 B6;C3Fe-Col2a1M2J/GrsrJ 006774 FVB-Tg(Col2a1-cre/ERT)KA3Smac/J 007657 STOCK Tg(Col2a1-Npr2*B2)25-4Gar/J View Strains carrying other alleles of Col2a1 (4 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Col2a1-cre)1Bhr | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Richard R Behringer | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Col2-Cre; Col2a1-Cre; | ||
| Mutation Made By | Dr. Richard Behringer, Univ of Texas, MD Anderson Cancer Center | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | differentiating chondrocytes, notochord, submandibular glands | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Col2a1-cre)1Bhr, transgene insertion 1, Richard R Behringer | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Col2-Cre; Col2a1-Cre; | ||
| Driver Note | Col2a1 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the Col2a1 promoter, which is active in differentiating chondrocytes, notochord and submandibular glands. [MGI Ref ID J:69318] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ovchinnikov DA; Deng JM; Ogunrinu G; Behringer RR. 2000. Col2a1-directed expression of Cre recombinase in differentiating chondrocytes in transgenic mice. Genesis 26(2):145-6. [PubMed: 10686612] [MGI Ref ID J:69318]
Additional References
Tg(Col2a1-cre)1Bhr relatedAhrens MJ; Romereim S; Dudley AT. 2011. A re-evaluation of two key reagents for in vivo studies of Wnt signaling. Dev Dyn :. [PubMed: 21793100] [MGI Ref ID J:174609]
Akiyama H; Chaboissier MC; Martin JF; Schedl A; De Crombrugghe B. 2002. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev 16(21):2813-28. [PubMed: 12414734] [MGI Ref ID J:79879]
Akiyama H; Lyons JP; Mori-Akiyama Y; Yang X; Zhang R; Zhang Z; Deng JM; Taketo MM; Nakamura T; Behringer RR; McCrea PD; de Crombrugghe B. 2004. Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev 18(9):1072-87. [PubMed: 15132997] [MGI Ref ID J:90567]
Arnold MA; Kim Y; Czubryt MP; Phan D; McAnally J; Qi X; Shelton JM; Richardson JA; Bassel-Duby R; Olson EN. 2007. MEF2C transcription factor controls chondrocyte hypertrophy and bone development. Dev Cell 12(3):377-89. [PubMed: 17336904] [MGI Ref ID J:119152]
Baffi MO; Moran MA; Serra R. 2006. Tgfbr2 regulates the maintenance of boundaries in the axial skeleton. Dev Biol 296(2):363-74. [PubMed: 16824508] [MGI Ref ID J:119316]
Baffi MO; Slattery E; Sohn P; Moses HL; Chytil A; Serra R. 2004. Conditional deletion of the TGF-beta type II receptor in Col2a expressing cells results in defects in the axial skeleton without alterations in chondrocyte differentiation or embryonic development of long bones. Dev Biol 276(1):124-42. [PubMed: 15531369] [MGI Ref ID J:95024]
Bentovim L; Amarilio R; Zelzer E. 2012. HIF1alpha is a central regulator of collagen hydroxylation and secretion under hypoxia during bone development. Development 139(23):4473-83. [PubMed: 23095889] [MGI Ref ID J:189214]
Chang W; Tu C; Chen TH; Bikle D; Shoback D. 2008. The extracellular calcium-sensing receptor (CaSR) is a critical modulator of skeletal development. Sci Signal 1(35):ra1. [PubMed: 18765830] [MGI Ref ID J:180651]
Deprez PM; Nichane MG; Rousseaux P; Devogelaer JP; Chappard D; Lengele BG; Rezsohazy R; Nyssen-Behets C. 2012. Postnatal growth defect in mice upon persistent Hoxa2 expression in the chondrogenic cell lineage. Differentiation 83(3):158-67. [PubMed: 22093256] [MGI Ref ID J:181027]
Downey CM; Horton CR; Carlson BA; Parsons TE; Hatfield DL; Hallgrimsson B; Jirik FR. 2009. Osteo-chondroprogenitor-specific deletion of the selenocysteine tRNA gene, Trsp, leads to chondronecrosis and abnormal skeletal development: a putative model for Kashin-Beck disease. PLoS Genet 5(8):e1000616. [PubMed: 19696890] [MGI Ref ID J:152152]
Dumitriu B; Dy P; Smits P; Lefebvre V. 2006. Generation of mice harboring a Sox6 conditional null allele. Genesis 44(5):219-24. [PubMed: 16652367] [MGI Ref ID J:110147]
Dy P; Smits P; Silvester A; Penzo-Mendez A; Dumitriu B; Han Y; de la Motte CA; Kingsley DM; Lefebvre V. 2010. Synovial joint morphogenesis requires the chondrogenic action of Sox5 and Sox6 in growth plate and articular cartilage. Dev Biol 341(2):346-59. [PubMed: 20206616] [MGI Ref ID J:160493]
Ford-Hutchinson AF; Ali Z; Lines SE; Hallgrimsson B; Boyd SK; Jirik FR. 2007. Inactivation of Pten in osteo-chondroprogenitor cells leads to epiphyseal growth plate abnormalities and skeletal overgrowth. J Bone Miner Res 22(8):1245-59. [PubMed: 17456009] [MGI Ref ID J:139422]
Govoni KE; Lee SK; Chung YS; Behringer RR; Wergedal JE; Baylink DJ; Mohan S. 2007. Disruption of insulin-like growth factor-I expression in type IIalphaI collagen-expressing cells reduces bone length and width in mice. Physiol Genomics 30(3):354-62. [PubMed: 17519362] [MGI Ref ID J:127222]
Grover J; Roughley PJ. 2006. Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. Matrix Biol 25(3):158-65. [PubMed: 16386413] [MGI Ref ID J:107728]
Guo X; Day TF; Jiang X; Garrett-Beal L; Topol L; Yang Y. 2004. Wnt/beta-catenin signaling is sufficient and necessary for synovial joint formation. Genes Dev 18(19):2404-17. [PubMed: 15371327] [MGI Ref ID J:93028]
Haberland M; Mokalled MH; Montgomery RL; Olson EN. 2009. Epigenetic control of skull morphogenesis by histone deacetylase 8. Genes Dev 23(14):1625-30. [PubMed: 19605684] [MGI Ref ID J:150709]
Hallgrimsson B; Lieberman DE; Liu W; Ford-Hutchinson AF; Jirik FR. 2007. Epigenetic interactions and the structure of phenotypic variation in the cranium. Evol Dev 9(1):76-91. [PubMed: 17227368] [MGI Ref ID J:147554]
Hinoi E; Bialek P; Chen YT; Rached MT; Groner Y; Behringer RR; Ornitz DM; Karsenty G. 2006. Runx2 inhibits chondrocyte proliferation and hypertrophy through its expression in the perichondrium. Genes Dev 20(21):2937-42. [PubMed: 17050674] [MGI Ref ID J:114686]
Hosaka Y; Saito T; Sugita S; Hikata T; Kobayashi H; Fukai A; Taniguchi Y; Hirata M; Akiyama H; Chung UI; Kawaguchi H. 2013. Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development. Proc Natl Acad Sci U S A 110(5):1875-80. [PubMed: 23319657] [MGI Ref ID J:193690]
Hsu SH; Zhang X; Yu C; Li ZJ; Wunder JS; Hui CC; Alman BA. 2011. Kif7 promotes hedgehog signaling in growth plate chondrocytes by restricting the inhibitory function of Sufu. Development 138(17):3791-801. [PubMed: 21795282] [MGI Ref ID J:175769]
Iwata T; Chen L; Li Cl; Ovchinnikov DA; Behringer RR; Francomano CA; Deng CX. 2000. A neonatal lethal mutation in FGFR3 uncouples proliferation and differentiation of growth plate chondrocytes in embryos Hum Mol Genet 9(11):1603-13. [PubMed: 10861287] [MGI Ref ID J:63198]
Jacob AL; Smith C; Partanen J; Ornitz DM. 2006. Fibroblast growth factor receptor 1 signaling in the osteo-chondrogenic cell lineage regulates sequential steps of osteoblast maturation. Dev Biol 296(2):315-28. [PubMed: 16815385] [MGI Ref ID J:119288]
Jones KB; Piombo V; Searby C; Kurriger G; Yang B; Grabellus F; Roughley PJ; Morcuende JA; Buckwalter JA; Capecchi MR; Vortkamp A; Sheffield VC. 2010. A mouse model of osteochondromagenesis from clonal inactivation of Ext1 in chondrocytes. Proc Natl Acad Sci U S A 107(5):2054-9. [PubMed: 20080592] [MGI Ref ID J:157599]
Keller B; Yang T; Chen Y; Munivez E; Bertin T; Zabel B; Lee B. 2011. Interaction of TGFbeta and BMP signaling pathways during chondrogenesis. PLoS One 6(1):e16421. [PubMed: 21297990] [MGI Ref ID J:180944]
Kim Y; Murao H; Yamamoto K; Deng JM; Behringer RR; Nakamura T; Akiyama H. 2011. Generation of transgenic mice for conditional overexpression of Sox9. J Bone Miner Metab 29(1):123-9. [PubMed: 20676705] [MGI Ref ID J:176952]
Kobayashi T; Lu J; Cobb BS; Rodda SJ; McMahon AP; Schipani E; Merkenschlager M; Kronenberg HM. 2008. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci U S A 105(6):1949-54. [PubMed: 18238902] [MGI Ref ID J:131828]
Koyama E; Shibukawa Y; Nagayama M; Sugito H; Young B; Yuasa T; Okabe T; Ochiai T; Kamiya N; Rountree RB; Kingsley DM; Iwamoto M; Enomoto-Iwamoto M; Pacifici M. 2008. A distinct cohort of progenitor cells participates in synovial joint and articular cartilage formation during mouse limb skeletogenesis. Dev Biol 316(1):62-73. [PubMed: 18295755] [MGI Ref ID J:135666]
Koyama E; Young B; Nagayama M; Shibukawa Y; Enomoto-Iwamoto M; Iwamoto M; Maeda Y; Lanske B; Song B; Serra R; Pacifici M. 2007. Conditional Kif3a ablation causes abnormal hedgehog signaling topography, growth plate dysfunction, and excessive bone and cartilage formation during mouse skeletogenesis. Development 134(11):2159-69. [PubMed: 17507416] [MGI Ref ID J:122610]
Lee HH; Behringer RR. 2007. Conditional expression of Wnt4 during chondrogenesis leads to dwarfism in mice. PLoS ONE 2(5):e450. [PubMed: 17505543] [MGI Ref ID J:129327]
Li Y; Ahrens MJ; Wu A; Liu J; Dudley AT. 2011. Calcium/calmodulin-dependent protein kinase II activity regulates the proliferative potential of growth plate chondrocytes. Development 138(2):359-70. [PubMed: 21177348] [MGI Ref ID J:180840]
Lin T; Sandusky SB; Xue H; Fishbein KW; Spencer RG; Rao MS; Francomano CA. 2003. A central nervous system specific mouse model for thanatophoric dysplasia type II. Hum Mol Genet 12(21):2863-71. [PubMed: 12966031] [MGI Ref ID J:86376]
Lincoln J; Kist R; Scherer G; Yutzey KE. 2007. Sox9 is required for precursor cell expansion and extracellular matrix organization during mouse heart valve development. Dev Biol 305(1):120-32. [PubMed: 17350610] [MGI Ref ID J:121352]
Lu C; Wan Y; Cao J; Zhu X; Yu J; Zhou R; Yao Y; Zhang L; Zhao H; Li H; Zhao J; He L; Ma G; Yang X; Yao Z; Guo X. 2013. Wnt-mediated reciprocal regulation between cartilage and bone development during endochondral ossification. Bone 53(2):566-74. [PubMed: 23274346] [MGI Ref ID J:193842]
Maes C; Goossens S; Bartunkova S; Drogat B; Coenegrachts L; Stockmans I; Moermans K; Nyabi O; Haigh K; Naessens M; Haenebalcke L; Tuckermann JP; Tjwa M; Carmeliet P; Mandic V; David JP; Behrens A; Nagy A; Carmeliet G; Haigh JJ. 2010. Increased skeletal VEGF enhances beta-catenin activity and results in excessively ossified bones. EMBO J 29(2):424-41. [PubMed: 20010698] [MGI Ref ID J:156474]
Mak KK; Chen MH; Day TF; Chuang PT; Yang Y. 2006. Wnt/{beta}-catenin signaling interacts differentially with Ihh signaling in controlling endochondral bone and synovial joint formation. Development 133(18):3695-707. [PubMed: 16936073] [MGI Ref ID J:112462]
Mak KK; Kronenberg HM; Chuang PT; Mackem S; Yang Y. 2008. Indian hedgehog signals independently of PTHrP to promote chondrocyte hypertrophy. Development 135(11):1947-56. [PubMed: 18434416] [MGI Ref ID J:134987]
Massip L; Ectors F; Deprez P; Maleki M; Behets C; Lengele B; Delahaut P; Picard J; Rezsohazy R. 2007. Expression of Hoxa2 in cells entering chondrogenesis impairs overall cartilage development. Differentiation 75(3):256-67. [PubMed: 17359301] [MGI Ref ID J:120083]
Masuyama R; Stockmans I; Torrekens S; Van Looveren R; Maes C; Carmeliet P; Bouillon R; Carmeliet G. 2006. Vitamin D receptor in chondrocytes promotes osteoclastogenesis and regulates FGF23 production in osteoblasts. J Clin Invest 116(12):3150-9. [PubMed: 17099775] [MGI Ref ID J:117377]
Matsushita T; Wilcox WR; Chan YY; Kawanami A; Bukulmez H; Balmes G; Krejci P; Mekikian PB; Otani K; Yamaura I; Warman ML; Givol D; Murakami S. 2009. FGFR3 promotes synchondrosis closure and fusion of ossification centers through the MAPK pathway. Hum Mol Genet 18(2):227-40. [PubMed: 18923003] [MGI Ref ID J:143273]
Mead TJ; Yutzey KE. 2009. Notch pathway regulation of chondrocyte differentiation and proliferation during appendicular and axial skeleton development. Proc Natl Acad Sci U S A 106(34):14420-5. [PubMed: 19590010] [MGI Ref ID J:151888]
Mi M; Jin H; Wang B; Yukata K; Sheu TJ; Ke QH; Tong P; Im HJ; Xiao G; Chen D. 2013. Chondrocyte BMP2 signaling plays an essential role in bone fracture healing. Gene 512(2):211-8. [PubMed: 23107765] [MGI Ref ID J:192162]
Moffatt P; Lee ER; St-Jacques B; Matsumoto K; Yamaguchi Y; Roughley PJ. 2011. Hyaluronan production by means of Has2 gene expression in chondrocytes is essential for long bone development. Dev Dyn 240(2):404-12. [PubMed: 21246657] [MGI Ref ID J:167831]
Moisan A; Rivera MN; Lotinun S; Akhavanfard S; Coffman EJ; Cook EB; Stoykova S; Mukherjee S; Schoonmaker JA; Burger A; Kim WJ; Kronenberg HM; Baron R; Haber DA; Bardeesy N. 2011. The WTX tumor suppressor regulates mesenchymal progenitor cell fate specification. Dev Cell 20(5):583-96. [PubMed: 21571217] [MGI Ref ID J:173242]
Mugniery E; Dacquin R; Marty C; Benoist-Lasselin C; de Vernejoul MC; Jurdic P; Munnich A; Geoffroy V; Legeai-Mallet L. 2012. An activating Fgfr3 mutation affects trabecular bone formation via a paracrine mechanism during growth. Hum Mol Genet 21(11):2503-13. [PubMed: 22367969] [MGI Ref ID J:183772]
Mundy C; Yasuda T; Kinumatsu T; Yamaguchi Y; Iwamoto M; Enomoto-Iwamoto M; Koyama E; Pacifici M. 2011. Synovial joint formation requires local Ext1 expression and heparan sulfate production in developing mouse embryo limbs and spine. Dev Biol 351(1):70-81. [PubMed: 21185280] [MGI Ref ID J:170582]
Nakamura E; Nguyen MT; Mackem S. 2006. Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev Dyn 235(9):2603-2612. [PubMed: 16894608] [MGI Ref ID J:111627]
Nishimura R; Wakabayashi M; Hata K; Matsubara T; Honma S; Wakisaka S; Kiyonari H; Shioi G; Yamaguchi A; Tsumaki N; Akiyama H; Yoneda T. 2012. Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification. J Biol Chem 287(40):33179-90. [PubMed: 22869368] [MGI Ref ID J:191589]
Nyabi O; Naessens M; Haigh K; Gembarska A; Goossens S; Maetens M; De Clercq S; Drogat B; Haenebalcke L; Bartunkova S; De Vos I; De Craene B; Karimi M; Berx G; Nagy A; Hilson P; Marine JC; Haigh JJ. 2009. Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells. Nucleic Acids Res 37(7):e55. [PubMed: 19279185] [MGI Ref ID J:194078]
Oh JH; Park SY; de Crombrugghe B; Kim JE. 2012. Chondrocyte-specific ablation of Osterix leads to impaired endochondral ossification. Biochem Biophys Res Commun 418(4):634-40. [PubMed: 22290230] [MGI Ref ID J:181485]
Oshima Y; Akiyama T; Hikita A; Iwasawa M; Nagase Y; Nakamura M; Wakeyama H; Kawamura N; Ikeda T; Chung UI; Hennighausen L; Kawaguchi H; Nakamura K; Tanaka S. 2008. Pivotal role of Bcl-2 family proteins in the regulation of chondrocyte apoptosis. J Biol Chem 283(39):26499-508. [PubMed: 18632667] [MGI Ref ID J:142327]
Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081] [MGI Ref ID J:164383]
Patra D; Xing X; Davies S; Bryan J; Franz C; Hunziker EB; Sandell LJ. 2007. Site-1 protease is essential for endochondral bone formation in mice. J Cell Biol 179(4):687-700. [PubMed: 18025304] [MGI Ref ID J:135374]
Peacock JD; Levay AK; Gillaspie DB; Tao G; Lincoln J. 2010. Reduced sox9 function promotes heart valve calcification phenotypes in vivo. Circ Res 106(4):712-9. [PubMed: 20056916] [MGI Ref ID J:170877]
Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285] [MGI Ref ID J:131172]
Provot S; Nachtrab G; Paruch J; Chen AP; Silva A; Kronenberg HM. 2008. A-raf and B-raf are dispensable for normal endochondral bone development, and parathyroid hormone-related peptide suppresses extracellular signal-regulated kinase activation in hypertrophic chondrocytes. Mol Cell Biol 28(1):344-57. [PubMed: 17967876] [MGI Ref ID J:128917]
Retting KN; Song B; Yoon BS; Lyons KM. 2009. BMP canonical Smad signaling through Smad1 and Smad5 is required for endochondral bone formation. Development 136(7):1093-104. [PubMed: 19224984] [MGI Ref ID J:147287]
Shim JH; Greenblatt MB; Xie M; Schneider MD; Zou W; Zhai B; Gygi S; Glimcher LH. 2009. TAK1 is an essential regulator of BMP signalling in cartilage. EMBO J 28(14):2028-41. [PubMed: 19536134] [MGI Ref ID J:150837]
Sohn P; Cox M; Chen D; Serra R. 2010. Molecular profiling of the developing mouse axial skeleton: a role for Tgfbr2 in the development of the intervertebral disc. BMC Dev Biol 10:29. [PubMed: 20214815] [MGI Ref ID J:160241]
Song B; Haycraft CJ; Seo HS; Yoder BK; Serra R. 2007. Development of the post-natal growth plate requires intraflagellar transport proteins. Dev Biol 305(1):202-16. [PubMed: 17359961] [MGI Ref ID J:121342]
Vincent SD; Dunn NR; Hayashi S; Norris DP; Robertson EJ. 2003. Cell fate decisions within the mouse organizer are governed by graded Nodal signals. Genes Dev 17(13):1646-62. [PubMed: 12842913] [MGI Ref ID J:84300]
Wang M; Jin H; Tang D; Huang S; Zuscik MJ; Chen D. 2011. Smad1 plays an essential role in bone development and postnatal bone formation. Osteoarthritis Cartilage 19(6):751-62. [PubMed: 21420501] [MGI Ref ID J:172689]
Wang Y; Serra R. 2012. PDGF mediates TGFbeta-induced migration during development of the spinous process. Dev Biol 365(1):110-7. [PubMed: 22369999] [MGI Ref ID J:184925]
Williams JA; Kondo N; Okabe T; Takeshita N; Pilchak DM; Koyama E; Ochiai T; Jensen D; Chu ML; Kane MA; Napoli JL; Enomoto-Iwamoto M; Ghyselinck N; Chambon P; Pacifici M; Iwamoto M. 2009. Retinoic acid receptors are required for skeletal growth, matrix homeostasis and growth plate function in postnatal mouse. Dev Biol 328(2):315-27. [PubMed: 19389355] [MGI Ref ID J:149466]
Xu X; Qiao W; Li C; Deng CX. 2002. Generation of Fgfr1 conditional knockout mice. Genesis 32(2):85-6. [PubMed: 11857785] [MGI Ref ID J:75137]
Yamamoto M; Matsuzaki T; Takahashi R; Adachi E; Maeda Y; Yamaguchi S; Kitayama H; Echizenya M; Morioka Y; Alexander DB; Yagi T; Itohara S; Nakamura T; Akiyama H; Noda M. 2012. The transformation suppressor gene Reck is required for postaxial patterning in mouse forelimbs Biol Open :458-466. [MGI Ref ID J:184585]
Yamashita S; Miyaki S; Kato Y; Yokoyama S; Sato T; Barrionuevo F; Akiyama H; Scherer G; Takada S; Asahara H. 2012. L-Sox5 and Sox6 proteins enhance chondrogenic miR-140 microRNA expression by strengthening dimeric Sox9 activity. J Biol Chem 287(26):22206-15. [PubMed: 22547066] [MGI Ref ID J:187536]
Yap SP; Xing X; Kraus P; Sivakamasundari V; Chan HY; Lufkin T. 2011. Generation of mice with a novel conditional null allele of the Sox9 gene. Biotechnol Lett 33(8):1551-8. [PubMed: 21484342] [MGI Ref ID J:176950]
Yoon BS; Ovchinnikov DA; Yoshii I; Mishina Y; Behringer RR; Lyons KM. 2005. Bmpr1a and Bmpr1b have overlapping functions and are essential for chondrogenesis in vivo. Proc Natl Acad Sci U S A 102(14):5062-7. [PubMed: 15781876] [MGI Ref ID J:97410]
Yoon BS; Pogue R; Ovchinnikov DA; Yoshii I; Mishina Y; Behringer RR; Lyons KM. 2006. BMPs regulate multiple aspects of growth-plate chondrogenesis through opposing actions on FGF pathways. Development 133(23):4667-78. [PubMed: 17065231] [MGI Ref ID J:119669]
Zhu H; Zhao J; Zhou W; Li H; Zhou R; Zhang L; Zhao H; Cao J; Zhu X; Hu H; Ma G; He L; Yao Z; Yao L; Guo X. 2012. Ndrg2 regulates vertebral specification in differentiating somites. Dev Biol 369(2):308-18. [PubMed: 22819676] [MGI Ref ID J:187603]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain was generated on a B6SJL/F1 background. The founder was crossd to B6 twice. Expected coat color from breeding:Black, Agouti Mating System +/+ sibling x Hemizygote (Female x Male) 01-MAR-11 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.