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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;J-Tg(Col2a1-cre)1Bhr/J (Changed: 15-DEC-04 ) B6;SJL-TgN(Col2a1-Cre)1Bhr (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Donating Investigator Richard Behringer, Univ of Texas, MD Anderson Cancer Center Description
This strain expresses Cre recombinase in a chondrocyte-specific pattern under the control of a Col2a1 promoter.Development
This strain was generated on a B6SJLF1 background. The founder was crossed to C57BL/6 twice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Col2a1
003916 B6(Cg)-Col2a1sedc/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J View Strains carrying other alleles of Col2a1 (2 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Col2a1-cre)1Bhr | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Richard R Behringer | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Col2-Cre; Col2a1-Cre; | ||
| Mutation Made By | Richard Behringer, Univ of Texas, MD Anderson Cancer Center | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | differentiating chondrocytes, notochord, submandibular glands | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory | ||
| Driver Note | Col2a1 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the Col2a1 promoter, which is active in differentiating chondrocytes, notochord and submandibular glands. [MGI Ref ID J:69318] | ||
| Gene Symbol and Name | Tg(Col2a1-cre)1Bhr, transgene insertion 1, Richard R Behringer | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Col2-Cre; Col2a1-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Ovchinnikov DA; Deng JM; Ogunrinu G; Behringer RR. 2000. Col2a1-directed expression of Cre recombinase in differentiating chondrocytes in transgenic mice. Genesis 26(2):145-6. [PubMed: 10686612] [MGI Ref ID J:69318]
Additional References
Tg(Col2a1-cre)1Bhr relatedAkiyama H; Chaboissier MC; Martin JF; Schedl A; De Crombrugghe B. 2002. The transcription factor Sox9 has essential roles in successive steps of the chondrocyte differentiation pathway and is required for expression of Sox5 and Sox6. Genes Dev 16(21):2813-28. [PubMed: 12414734] [MGI Ref ID J:79879]
Akiyama H; Lyons JP; Mori-Akiyama Y; Yang X; Zhang R; Zhang Z; Deng JM; Taketo MM; Nakamura T; Behringer RR; McCrea PD; de Crombrugghe B. 2004. Interactions between Sox9 and beta-catenin control chondrocyte differentiation. Genes Dev 18(9):1072-87. [PubMed: 15132997] [MGI Ref ID J:90567]
Arnold MA; Kim Y; Czubryt MP; Phan D; McAnally J; Qi X; Shelton JM; Richardson JA; Bassel-Duby R; Olson EN. 2007. MEF2C transcription factor controls chondrocyte hypertrophy and bone development. Dev Cell 12(3):377-89. [PubMed: 17336904] [MGI Ref ID J:119152]
Baffi MO; Moran MA; Serra R. 2006. Tgfbr2 regulates the maintenance of boundaries in the axial skeleton. Dev Biol 296(2):363-74. [PubMed: 16824508] [MGI Ref ID J:119316]
Baffi MO; Slattery E; Sohn P; Moses HL; Chytil A; Serra R. 2004. Conditional deletion of the TGF-beta type II receptor in Col2a expressing cells results in defects in the axial skeleton without alterations in chondrocyte differentiation or embryonic development of long bones. Dev Biol 276(1):124-42. [PubMed: 15531369] [MGI Ref ID J:95024]
Downey CM; Horton CR; Carlson BA; Parsons TE; Hatfield DL; Hallgrimsson B; Jirik FR. 2009. Osteo-chondroprogenitor-specific deletion of the selenocysteine tRNA gene, Trsp, leads to chondronecrosis and abnormal skeletal development: a putative model for Kashin-Beck disease. PLoS Genet 5(8):e1000616. [PubMed: 19696890] [MGI Ref ID J:152152]
Dumitriu B; Dy P; Smits P; Lefebvre V. 2006. Generation of mice harboring a Sox6 conditional null allele. Genesis 44(5):219-24. [PubMed: 16652367] [MGI Ref ID J:110147]
Ford-Hutchinson AF; Ali Z; Lines SE; Hallgrimsson B; Boyd SK; Jirik FR. 2007. Inactivation of Pten in osteo-chondroprogenitor cells leads to epiphyseal growth plate abnormalities and skeletal overgrowth. J Bone Miner Res 22(8):1245-59. [PubMed: 17456009] [MGI Ref ID J:139422]
Govoni KE; Lee SK; Chung YS; Behringer RR; Wergedal JE; Baylink DJ; Mohan S. 2007. Disruption of insulin-like growth factor-I expression in type IIalphaI collagen-expressing cells reduces bone length and width in mice. Physiol Genomics 30(3):354-62. [PubMed: 17519362] [MGI Ref ID J:127222]
Grover J; Roughley PJ. 2006. Generation of a transgenic mouse in which Cre recombinase is expressed under control of the type II collagen promoter and doxycycline administration. Matrix Biol 25(3):158-65. [PubMed: 16386413] [MGI Ref ID J:107728]
Guo X; Day TF; Jiang X; Garrett-Beal L; Topol L; Yang Y. 2004. Wnt/beta-catenin signaling is sufficient and necessary for synovial joint formation. Genes Dev 18(19):2404-17. [PubMed: 15371327] [MGI Ref ID J:93028]
Haberland M; Mokalled MH; Montgomery RL; Olson EN. 2009. Epigenetic control of skull morphogenesis by histone deacetylase 8. Genes Dev 23(14):1625-30. [PubMed: 19605684] [MGI Ref ID J:150709]
Hallgrimsson B; Lieberman DE; Liu W; Ford-Hutchinson AF; Jirik FR. 2007. Epigenetic interactions and the structure of phenotypic variation in the cranium. Evol Dev 9(1):76-91. [PubMed: 17227368] [MGI Ref ID J:147554]
Hinoi E; Bialek P; Chen YT; Rached MT; Groner Y; Behringer RR; Ornitz DM; Karsenty G. 2006. Runx2 inhibits chondrocyte proliferation and hypertrophy through its expression in the perichondrium. Genes Dev 20(21):2937-42. [PubMed: 17050674] [MGI Ref ID J:114686]
Iwata T; Chen L; Li Cl; Ovchinnikov DA; Behringer RR; Francomano CA; Deng CX. 2000. A neonatal lethal mutation in FGFR3 uncouples proliferation and differentiation of growth plate chondrocytes in embryos Hum Mol Genet 9(11):1603-13. [PubMed: 10861287] [MGI Ref ID J:63198]
Jacob AL; Smith C; Partanen J; Ornitz DM. 2006. Fibroblast growth factor receptor 1 signaling in the osteo-chondrogenic cell lineage regulates sequential steps of osteoblast maturation. Dev Biol 296(2):315-28. [PubMed: 16815385] [MGI Ref ID J:119288]
Kobayashi T; Lu J; Cobb BS; Rodda SJ; McMahon AP; Schipani E; Merkenschlager M; Kronenberg HM. 2008. Dicer-dependent pathways regulate chondrocyte proliferation and differentiation. Proc Natl Acad Sci U S A 105(6):1949-54. [PubMed: 18238902] [MGI Ref ID J:131828]
Koyama E; Shibukawa Y; Nagayama M; Sugito H; Young B; Yuasa T; Okabe T; Ochiai T; Kamiya N; Rountree RB; Kingsley DM; Iwamoto M; Enomoto-Iwamoto M; Pacifici M. 2008. A distinct cohort of progenitor cells participates in synovial joint and articular cartilage formation during mouse limb skeletogenesis. Dev Biol 316(1):62-73. [PubMed: 18295755] [MGI Ref ID J:135666]
Koyama E; Young B; Nagayama M; Shibukawa Y; Enomoto-Iwamoto M; Iwamoto M; Maeda Y; Lanske B; Song B; Serra R; Pacifici M. 2007. Conditional Kif3a ablation causes abnormal hedgehog signaling topography, growth plate dysfunction, and excessive bone and cartilage formation during mouse skeletogenesis. Development 134(11):2159-69. [PubMed: 17507416] [MGI Ref ID J:122610]
Lee HH; Behringer RR. 2007. Conditional expression of Wnt4 during chondrogenesis leads to dwarfism in mice. PLoS ONE 2(5):e450. [PubMed: 17505543] [MGI Ref ID J:129327]
Lin T; Sandusky SB; Xue H; Fishbein KW; Spencer RG; Rao MS; Francomano CA. 2003. A central nervous system specific mouse model for thanatophoric dysplasia type II. Hum Mol Genet 12(21):2863-71. [PubMed: 12966031] [MGI Ref ID J:86376]
Lincoln J; Kist R; Scherer G; Yutzey KE. 2007. Sox9 is required for precursor cell expansion and extracellular matrix organization during mouse heart valve development. Dev Biol 305(1):120-32. [PubMed: 17350610] [MGI Ref ID J:121352]
Mak KK; Chen MH; Day TF; Chuang PT; Yang Y. 2006. Wnt/{beta}-catenin signaling interacts differentially with Ihh signaling in controlling endochondral bone and synovial joint formation. Development 133(18):3695-707. [PubMed: 16936073] [MGI Ref ID J:112462]
Mak KK; Kronenberg HM; Chuang PT; Mackem S; Yang Y. 2008. Indian hedgehog signals independently of PTHrP to promote chondrocyte hypertrophy. Development 135(11):1947-56. [PubMed: 18434416] [MGI Ref ID J:134987]
Massip L; Ectors F; Deprez P; Maleki M; Behets C; Lengele B; Delahaut P; Picard J; Rezsohazy R. 2007. Expression of Hoxa2 in cells entering chondrogenesis impairs overall cartilage development. Differentiation 75(3):256-67. [PubMed: 17359301] [MGI Ref ID J:120083]
Masuyama R; Stockmans I; Torrekens S; Van Looveren R; Maes C; Carmeliet P; Bouillon R; Carmeliet G. 2006. Vitamin D receptor in chondrocytes promotes osteoclastogenesis and regulates FGF23 production in osteoblasts. J Clin Invest 116(12):3150-9. [PubMed: 17099775] [MGI Ref ID J:117377]
Matsushita T; Wilcox WR; Chan YY; Kawanami A; Bukulmez H; Balmes G; Krejci P; Mekikian PB; Otani K; Yamaura I; Warman ML; Givol D; Murakami S. 2009. FGFR3 promotes synchondrosis closure and fusion of ossification centers through the MAPK pathway. Hum Mol Genet 18(2):227-40. [PubMed: 18923003] [MGI Ref ID J:143273]
Mead TJ; Yutzey KE. 2009. Notch pathway regulation of chondrocyte differentiation and proliferation during appendicular and axial skeleton development. Proc Natl Acad Sci U S A 106(34):14420-5. [PubMed: 19590010] [MGI Ref ID J:151888]
Oshima Y; Akiyama T; Hikita A; Iwasawa M; Nagase Y; Nakamura M; Wakeyama H; Kawamura N; Ikeda T; Chung UI; Hennighausen L; Kawaguchi H; Nakamura K; Tanaka S. 2008. Pivotal role of Bcl-2 family proteins in the regulation of chondrocyte apoptosis. J Biol Chem 283(39):26499-508. [PubMed: 18632667] [MGI Ref ID J:142327]
Patra D; Xing X; Davies S; Bryan J; Franz C; Hunziker EB; Sandell LJ. 2007. Site-1 protease is essential for endochondral bone formation in mice. J Cell Biol 179(4):687-700. [PubMed: 18025304] [MGI Ref ID J:135374]
Pickett EA; Olsen GS; Tallquist MD. 2008. Disruption of PDGFR{alpha}-initiated PI3K activation and migration of somite derivatives leads to spina bifida. Development 135(3):589-98. [PubMed: 18192285] [MGI Ref ID J:131172]
Provot S; Nachtrab G; Paruch J; Chen AP; Silva A; Kronenberg HM. 2008. A-raf and B-raf are dispensable for normal endochondral bone development, and parathyroid hormone-related peptide suppresses extracellular signal-regulated kinase activation in hypertrophic chondrocytes. Mol Cell Biol 28(1):344-57. [PubMed: 17967876] [MGI Ref ID J:128917]
Retting KN; Song B; Yoon BS; Lyons KM. 2009. BMP canonical Smad signaling through Smad1 and Smad5 is required for endochondral bone formation. Development 136(7):1093-104. [PubMed: 19224984] [MGI Ref ID J:147287]
Shim JH; Greenblatt MB; Xie M; Schneider MD; Zou W; Zhai B; Gygi S; Glimcher LH. 2009. TAK1 is an essential regulator of BMP signalling in cartilage. EMBO J 28(14):2028-41. [PubMed: 19536134] [MGI Ref ID J:150837]
Song B; Haycraft CJ; Seo HS; Yoder BK; Serra R. 2007. Development of the post-natal growth plate requires intraflagellar transport proteins. Dev Biol 305(1):202-16. [PubMed: 17359961] [MGI Ref ID J:121342]
Vincent SD; Dunn NR; Hayashi S; Norris DP; Robertson EJ. 2003. Cell fate decisions within the mouse organizer are governed by graded Nodal signals. Genes Dev 17(13):1646-62. [PubMed: 12842913] [MGI Ref ID J:84300]
Williams JA; Kondo N; Okabe T; Takeshita N; Pilchak DM; Koyama E; Ochiai T; Jensen D; Chu ML; Kane MA; Napoli JL; Enomoto-Iwamoto M; Ghyselinck N; Chambon P; Pacifici M; Iwamoto M. 2009. Retinoic acid receptors are required for skeletal growth, matrix homeostasis and growth plate function in postnatal mouse. Dev Biol 328(2):315-27. [PubMed: 19389355] [MGI Ref ID J:149466]
Xu X; Qiao W; Li C; Deng CX. 2002. Generation of Fgfr1 conditional knockout mice. Genesis 32(2):85-6. [PubMed: 11857785] [MGI Ref ID J:75137]
Yoon BS; Ovchinnikov DA; Yoshii I; Mishina Y; Behringer RR; Lyons KM. 2005. Bmpr1a and Bmpr1b have overlapping functions and are essential for chondrogenesis in vivo. Proc Natl Acad Sci U S A 102(14):5062-7. [PubMed: 15781876] [MGI Ref ID J:97410]
Yoon BS; Pogue R; Ovchinnikov DA; Yoshii I; Mishina Y; Behringer RR; Lyons KM. 2006. BMPs regulate multiple aspects of growth-plate chondrogenesis through opposing actions on FGF pathways. Development 133(23):4667-78. [PubMed: 17065231] [MGI Ref ID J:119669]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain was generated on a B6SJL/F1 background. The founder was crossd to B6 twice. Expected coat color from breeding:Black, Agouti Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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