Strain Name: |
B6;SJL-Tg(Col2a1-cre)1Bhr/J |
|---|---|
Stock Number: |
003554 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6;J-Tg(Col2a1-cre)1Bhr/J (Changed: 15-DEC-04
) |
|
B6;SJL-TgN(Col2a1-Cre)1Bhr (Changed: 15-DEC-04
) | |
| Genes & Alleles | Col2a1; cre; Tg(Col2a1-cre)1Bhr; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Richard Behringer, Univ of Texas, MD Anderson Cancer Center Generation N1F1p+N1p (22-JAN-06) Strain Description
This strain expresses Cre recombinase in a chondrocyte-specific pattern under the control of a Col2a1 promoter.Strain Development
This strain was generated on a B6SJLF1 background. The founder was crossed to C57BL/6 twice.
Gene & Allele Details
| Allele Symbol | Tg(Col2a1-cre)1Bhr | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Richard R Behringer | ||
| Common Name(s) | Col2-Cre; Col2a1-Cre; | ||
| Mutation Made By | Richard Behringer, Univ of Texas, MD Anderson Cancer Center | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | differentiating chondrocytes, notochord, submandibular glands | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Col2a1, collagen, type II, alpha 1, mouse, laboratory | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the Col2a1 promoter, which is active in differentiating chondrocytes, notochord and submandibular glands. [MGI Ref ID J:69318] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | This strain was generated on a B6SJL/F1 background. The founder was crossd to B6 twice. Expected coat color from breeding:Black, Agouti |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Col2a1
003916 B6(Cg)-Col2a1sedc/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J View Strains carrying other alleles of Col2a1 (2 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesOvchinnikov DA; Deng JM; Ogunrinu G; Behringer RR. 2000. Col2a1-directed expression of Cre recombinase in differentiating chondrocytes in transgenic mice. Genesis 26(2):145-6. [PubMed: 10686612] [MGI Ref ID J:69318]
Price and Supply Information
| Strain Name: | B6;SJL-Tg(Col2a1-cre)1Bhr/J |
| Stock Number: | 003554 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $1900.00 | ||||
| Cryopreserved Embryos Fee | $1600.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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