Strain Name:

B6.129-Grm5tm1Rod/J

Stock Number:

003558

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129-Gprc1etm1Rod    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Long-term potentiation (LTP) in Grm5tm1Rod (mGluR5) targeted mutant mice was significantly reduced in the NMDA receptor (NMDAR)-dependent pathways such as the CA1 region and dentate gyrus of the hippocampus, whereas LTP remained intact in the mossy fiber synapses on the CA3 region, an NMDAR-independent pathway. These mutant mice were also impaired in the acquisition and use of spatial information in both the Morris water maze and contextual information in the fear-conditioning test. Homozygotes are viable and fertile.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Related Strains

Strains carrying   Grm5tm1Rod allele
003121   B6;129-Grm5tm1Rod/J
View Strains carrying   Grm5tm1Rod     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Grm5tm1Rod/Grm5+

        involves: 129 * C57BL/6 * FVB
  • nervous system phenotype
  • abnormal nerve fiber response
    • deprived-eye depression is reduced following monocular depression in comparison to controls   (MGI Ref ID J:127792)

Grm5tm1Rod/Grm5tm1Rod

        B6.129-Grm5tm1Rod/J
  • behavior/neurological phenotype
  • abnormal locomotor activation
    • mutants do not show any locomotor stimulation in response to a Grm5, MPEP, antagonist whereas wild-type littermates display increased locomotor activity   (MGI Ref ID J:102700)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Grm5tm1Rod/Grm5tm1Rod

        involves: 129S1/Sv * 129X1/SvJ * CD-1
  • nervous system phenotype
  • abnormal excitatory postsynaptic potential
    • field excitatory postsynaptic potential (fEPSP) depression by (1S,3R)-1-amino-cyclopentane-1,3-dicarboxylic acid (ACPD) is attenuated (12+/-10% compared to 55.5+/-4.6% in wild-type mice at 50uM ACPD)   (MGI Ref ID J:41096)
    • however, fEPSP depression by ACPD at 300uM is normal   (MGI Ref ID J:41096)
  • reduced long term potentiation
    • significant reduction in long term depression (LTP) in NMDA receptor-dependent pathways, such as the CA1 region and dentate gyrus of the hippocampus, but not in NMDA receptor-independent pathways, such as the mossy fiber synapses of the CA3 region   (MGI Ref ID J:41096)
    • LTP in the last 30 minutes after tetanus was 50.2+/-5.7% above baseline compared to 77.6+/-10.2% above base line in wild-type mice   (MGI Ref ID J:41096)
  • behavior/neurological phenotype
  • abnormal contextual conditioning behavior
    • mice exhibit a decrease in contextual fear conditioning   (MGI Ref ID J:41096)
  • abnormal spatial learning
    • mice exhibit decreased learning acquisition in a hidden platform water maze   (MGI Ref ID J:41096)
    • in a water maze, mice spend less time searching for the target quadrant and cross the platform less frequently when compared with wild-type mice   (MGI Ref ID J:41096)
    • however, locomotion and performance in an open field test are normal   (MGI Ref ID J:41096)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Receptor Defects
      glutamate receptor: metabotropic

Grm5tm1Rod related

Neurobiology Research
Behavioral and Learning Defects
Receptor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Grm5tm1Rod
Allele Name targeted mutation 1, John C Roder
Allele Type Targeted (Null/Knockout)
Common Name(s) Gprc1etm1Rod; mGluR5-;
Mutation Made ByDr. John Roder,   University of Toronto
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Grm5, glutamate receptor, metabotropic 5
Chromosome 7
Gene Common Name(s) 6430542K11Rik; AI850523; G protein-coupled receptor, family C, group 1, member E; GPRC1E; Glu5R; Gprc1e; MGLUR5; PPP1R86; RIKEN cDNA 6430542K11 gene; expressed sequence AI850523; mGlu5;
Molecular Note A targeting vector was designed to delete a 0.4 kb fragment containing part of exon 1 and a part of intron 1, with a neomycin resistance cassette. Western blot failed to detect protein in mutants. [MGI Ref ID J:51151]

Genotyping

Genotyping Information

Genotyping Protocols

Grm5tm1Rod, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Lu YM; Jia Z; Janus C; Henderson JT; Gerlai R; Wojtowicz JM; Roder JC. 1997. Mice lacking metabotropic glutamate receptor 5 show impaired learning and reduced CA1 long-term potentiation (LTP) but normal CA3 LTP. J Neurosci 17(13):5196-205. [PubMed: 9185557]  [MGI Ref ID J:41096]

Additional References

Grm5tm1Rod related

Ballester-Rosado CJ; Albright MJ; Wu CS; Liao CC; Zhu J; Xu J; Lee LJ; Lu HC. 2010. mGluR5 in cortical excitatory neurons exerts both cell-autonomous and -nonautonomous influences on cortical somatosensory circuit formation. J Neurosci 30(50):16896-909. [PubMed: 21159961]  [MGI Ref ID J:167595]

Bortolotto ZA; Collett VJ; Conquet F; Jia Z; van der Putten H; Collingridge GL. 2005. The regulation of hippocampal LTP by the molecular switch, a form of metaplasticity, requires mGlu5 receptors. Neuropharmacology 49 Suppl 1:13-25. [PubMed: 16024054]  [MGI Ref ID J:112751]

Brodkin J; Bradbury M; Busse C; Warren N; Bristow LJ; Varney MA. 2002. Reduced stress-induced hyperthermia in mGluR5 knockout mice. Eur J Neurosci 16(11):2241-4. [PubMed: 12473093]  [MGI Ref ID J:108073]

Chen CC; Lu HC; Brumberg JC. 2012. mGluR5 knockout mice display increased dendritic spine densities. Neurosci Lett 524(1):65-8. [PubMed: 22819970]  [MGI Ref ID J:188790]

Chesworth R; Brown RM; Kim JH; Lawrence AJ. 2013. The metabotropic glutamate 5 receptor modulates extinction and reinstatement of methamphetamine-seeking in mice. PLoS One 8(7):e68371. [PubMed: 23861896]  [MGI Ref ID J:204284]

Di Giorgi-Gerevini V; Melchiorri D; Battaglia G; Ricci-Vitiani L; Ciceroni C; Busceti CL; Biagioni F; Iacovelli L; Canudas AM; Parati E; De Maria R; Nicoletti F. 2005. Endogenous activation of metabotropic glutamate receptors supports the proliferation and survival of neural progenitor cells. Cell Death Differ 12(8):1124-33. [PubMed: 15947794]  [MGI Ref ID J:116384]

Dolen G; Osterweil E; Rao BS; Smith GB; Auerbach BD; Chattarji S; Bear MF. 2007. Correction of Fragile X Syndrome in Mice. Neuron 56(6):955-962. [PubMed: 18093519]  [MGI Ref ID J:127792]

Downing C; Marks MJ; Larson C; Johnson TE. 2010. The metabotropic glutamate receptor subtype 5 mediates sensitivity to the sedative properties of ethanol. Pharmacogenet Genomics 20(9):553-64. [PubMed: 20657349]  [MGI Ref ID J:180856]

Hannan AJ; Blakemore C; Katsnelson A; Vitalis T; Huber KM; Bear M; Roder J; Kim D; Shin HS; Kind PC. 2001. PLC-beta1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex. Nat Neurosci 4(3):282-8. [PubMed: 11224545]  [MGI Ref ID J:109291]

Hays SA; Huber KM; Gibson JR. 2011. Altered Neocortical Rhythmic Activity States in Fmr1 KO Mice Are Due to Enhanced mGluR5 Signaling and Involve Changes in Excitatory Circuitry. J Neurosci 31(40):14223-34. [PubMed: 21976507]  [MGI Ref ID J:177183]

Hu JH; Yang L; Kammermeier PJ; Moore CG; Brakeman PR; Tu J; Yu S; Petralia RS; Li Z; Zhang PW; Park JM; Dong X; Xiao B; Worley PF. 2012. Preso1 dynamically regulates group I metabotropic glutamate receptors. Nat Neurosci 15(6):836-44. [PubMed: 22561452]  [MGI Ref ID J:191366]

Huber KM; Roder JC; Bear MF. 2001. Chemical induction of mGluR5- and protein synthesis--dependent long-term depression in hippocampal area CA1. J Neurophysiol 86(1):321-5. [PubMed: 11431513]  [MGI Ref ID J:103903]

Inta D; Vogt MA; Luoni A; Filipovic D; Lima-Ojeda JM; Pfeiffer N; Gasparini F; Riva MA; Gass P. 2013. Significant increase in anxiety during aging in mGlu5 receptor knockout mice. Behav Brain Res 241:27-31. [PubMed: 23228523]  [MGI Ref ID J:197069]

Jia Z; Lu Y; Henderson J; Taverna F; Romano C; Abramow-Newerly W; Wojtowicz JM; Roder J. 1998. Selective abolition of the NMDA component of long-term potentiation in mice lacking mGluR5. Learn Mem 5(4-5):331-43. [PubMed: 10454358]  [MGI Ref ID J:51151]

Kachroo A; Orlando LR; Grandy DK; Chen JF; Young AB; Schwarzschild MA. 2005. Interactions between metabotropic glutamate 5 and adenosine A2A receptors in normal and parkinsonian mice. J Neurosci 25(45):10414-9. [PubMed: 16280580]  [MGI Ref ID J:102700]

Kinney GG; Burno M; Campbell UC; Hernandez LM; Rodriguez D; Bristow LJ; Conn PJ. 2003. Metabotropic glutamate subtype 5 receptors modulate locomotor activity and sensorimotor gating in rodents. J Pharmacol Exp Ther 306(1):116-23. [PubMed: 12660307]  [MGI Ref ID J:124739]

Kolber BJ; Montana MC; Carrasquillo Y; Xu J; Heinemann SF; Muglia LJ; Gereau RW 4th. 2010. Activation of metabotropic glutamate receptor 5 in the amygdala modulates pain-like behavior. J Neurosci 30(24):8203-13. [PubMed: 20554871]  [MGI Ref ID J:161801]

Marin YE; Namkoong J; Shin SS; Raines J; Degenhardt K; White E; Chen S. 2005. Grm5 expression is not required for the oncogenic role of Grm1 in melanocytes. Neuropharmacology 49 Suppl 1:70-9. [PubMed: 16040064]  [MGI Ref ID J:179570]

Musante V; Neri E; Feligioni M; Puliti A; Pedrazzi M; Conti V; Usai C; Diaspro A; Ravazzolo R; Henley JM; Battaglia G; Pittaluga A. 2008. Presynaptic mGlu1 and mGlu5 autoreceptors facilitate glutamate exocytosis from mouse cortical nerve endings. Neuropharmacology 55(4):474-82. [PubMed: 18625255]  [MGI Ref ID J:179420]

Olsen CM; Childs DS; Stanwood GD; Winder DG. 2010. Operant sensation seeking requires metabotropic glutamate receptor 5 (mGluR5). PLoS One 5(11):e15085. [PubMed: 21152045]  [MGI Ref ID J:167278]

Ribeiro FM; Devries RA; Hamilton A; Guimaraes IM; Cregan SP; Pires RG; Ferguson SS. 2014. Metabotropic glutamate receptor 5 knockout promotes motor and biochemical alterations in a mouse model of Huntington's disease. Hum Mol Genet 23(8):2030-42. [PubMed: 24282028]  [MGI Ref ID J:207093]

Ribeiro FM; Paquet M; Ferreira LT; Cregan T; Swan P; Cregan SP; Ferguson SS. 2010. Metabotropic glutamate receptor-mediated cell signaling pathways are altered in a mouse model of Huntington's disease. J Neurosci 30(1):316-24. [PubMed: 20053912]  [MGI Ref ID J:164039]

She WC; Quairiaux C; Albright MJ; Wang YC; Sanchez DE; Chang PS; Welker E; Lu HC. 2009. Roles of mGluR5 in synaptic function and plasticity of the mouse thalamocortical pathway. Eur J Neurosci 29(7):1379-96. [PubMed: 19519626]  [MGI Ref ID J:150200]

Sibaev A; Yuce B; Kemmer M; Van Nassauw L; Broedl U; Allescher HD; Goke B; Timmermans JP; Storr M. 2009. Cannabinoid-1 (CB1) receptors regulate colonic propulsion by acting at motor neurons within the ascending motor pathways in mouse colon. Am J Physiol Gastrointest Liver Physiol 296(1):G119-28. [PubMed: 19033531]  [MGI Ref ID J:143062]

Sidiropoulou K; Lu FM; Fowler MA; Xiao R; Phillips C; Ozkan ED; Zhu MX; White FJ; Cooper DC. 2009. Dopamine modulates an mGluR5-mediated depolarization underlying prefrontal persistent activity. Nat Neurosci 12(2):190-9. [PubMed: 19169252]  [MGI Ref ID J:146136]

Storto M; Capobianco L; Battaglia G; Molinaro G; Gradini R; Riozzi B; Di Mambro A; Mitchell KJ; Bruno V; Vairetti MP; Rutter GA; Nicoletti F. 2006. Insulin secretion is controlled by mGlu5 metabotropic glutamate receptors. Mol Pharmacol 69(4):1234-41. [PubMed: 16424079]  [MGI Ref ID J:135799]

Takasaki C; Okada R; Mitani A; Fukaya M; Yamasaki M; Fujihara Y; Shirakawa T; Tanaka K; Watanabe M. 2008. Glutamate transporters regulate lesion-induced plasticity in the developing somatosensory cortex. J Neurosci 28(19):4995-5006. [PubMed: 18463253]  [MGI Ref ID J:135189]

Um JW; Kaufman AC; Kostylev M; Heiss JK; Stagi M; Takahashi H; Kerrisk ME; Vortmeyer A; Wisniewski T; Koleske AJ; Gunther EC; Nygaard HB; Strittmatter SM. 2013. Metabotropic glutamate receptor 5 is a coreceptor for Alzheimer abeta oligomer bound to cellular prion protein. Neuron 79(5):887-902. [PubMed: 24012003]  [MGI Ref ID J:201741]

Xiao XL; Ma DL; Wu J; Tang FR. 2013. Metabotropic glutamate receptor 5 (mGluR5) regulates proliferation and differentiation of neuronal progenitors in the developmental hippocampus. Brain Res 1493:1-12. [PubMed: 23165119]  [MGI Ref ID J:206552]

Zhang L; Alger BE. 2010. Enhanced endocannabinoid signaling elevates neuronal excitability in fragile X syndrome. J Neurosci 30(16):5724-9. [PubMed: 20410124]  [MGI Ref ID J:159835]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

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