Strain Name: |
B6.Cg-Tg(Ins2-cre)25Mgn/J |
|---|---|
Stock Number: |
003573 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6-Tg(Ins2-cre)25Mgn/J (Changed: 15-DEC-04
) |
|
C57BL/6-TgN(Ins2-Cre)25Mgn (Changed: 15-DEC-04
) | |
|
C57BL/6-TgN(Ins2Cre)25Mgn (Changed: 15-DEC-04
) | |
| Genes & Alleles | Ins2; cre; Tg(Ins2-cre)25Mgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Mark Magnuson, Vanderbilt University School of Medicine Generation N12+1F15 (08-JAN-08) Strain Description
This strain carries the "RIP-Cre" transgene construct (containing a 668 bp fragment of the rat insulin II promoter, Cre recombinase with a nuclear localization sequence, and a 2.1 kb fragment from the human growth hormone gene). Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. This transgene strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the "Cre-lox" system. Results from several different laboratories have shown that this transgenic strain is at least 85% efficient in achieving pancreatic beta cell-specific recombination. It should also be noted that the transgene in this line has been found to be expressed at a low level in the hypothalamus. In some cases this has resulted in a phenotype due to deletion of the floxed allele in this region of the brain. It has also been shown that these transgenic mice may spontaneously develop glucose intolerance and impaired insulin secretion developing at 6-8 weeks of age. It is recommended that users include naive "RIP-Cre" mice (i.e., those not bred to a floxed mutant) among the controls used in experiments.Strain Development
A transgenic construct containing a 668 bp rat insulin II promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kb fragment from the human growth hormone gene was injected into B6D2(F2) pronuclei. The allele was subsequently moved to a C57BL/6J background.
Gene & Allele Details
| Allele Symbol | Tg(Ins2-cre)25Mgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 25, Mark A Magnuson | ||
| Common Name(s) | Ins-Cre; Ins2cre; RIP Cre; RIP2-cre; Rip-cre; [RIP]-Cre; | ||
| Strain of Origin | (C57BL/6 x DBA)F2 | ||
| Site of Expression | pancreatic beta cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the rat insulin promoter (Ins2), which is active in pancreatic beta cells and in neurons of the hypothalmus. [MGI Ref ID J:51826] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | This strain was originally constructed on a C57BL/6 X DBA2 background. They are currently being maintained as hemizygotes by matings with C57BL/6J. Expected coat color from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
JAX Notes, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Diabetes and Obesity Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesPostic C; Shiota M; Niswender KD; Jetton TL; Chen Y; Moates JM; Shelton KD; Lindner J; Cherrington AD; Magnuson MA. 1999. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J Biol Chem 274(1):305-15. [PubMed: 9867845] [MGI Ref ID J:51826]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Ins2-cre)25Mgn/J |
| Stock Number: | 003573 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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