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- Description
- Phenotype
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Description
Strain Information
Former Names C57BL/6-Tg(Ins2-cre)25Mgn/J (Changed: 15-DEC-04 ) C57BL/6-TgN(Ins2-Cre)25Mgn (Changed: 15-DEC-04 ) C57BL/6-TgN(Ins2Cre)25Mgn (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) 03-SEP-08 Species laboratory mouse Generation N12+1F18 (22-JAN-09)
Generation DefinitionsDonating Investigator Mark Magnuson, Vanderbilt University School of Medicine Description
This strain carries the "RIP-Cre" transgene construct (containing a 668 bp fragment of the rat insulin II promoter, Cre recombinase with a nuclear localization sequence, and a 2.1 kb fragment from the human growth hormone gene). Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. This transgene strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the "Cre-lox" system. Results from several different laboratories have shown that this transgenic strain is at least 85% efficient in achieving pancreatic beta cell-specific recombination. It should also be noted that the transgene in this line has been found to be expressed at a low level in the hypothalamus. In some cases this has resulted in a phenotype due to deletion of the floxed allele in this region of the brain. It has also been shown that these transgenic mice may spontaneously develop glucose intolerance and impaired insulin secretion developing at 6-8 weeks of age. It is recommended that users include naive "RIP-Cre" mice (i.e., those not bred to a floxed mutant) among the controls used in experiments.Development
A transgenic construct containing a 668 bp rat insulin II promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kb fragment from the human growth hormone gene was injected into B6D2(F2) pronuclei. The allele was subsequently moved to a C57BL/6J background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (46 strains)
Additional Web Information
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
JAX® NOTES, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Diabetes and Obesity Research
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Ins2-cre)25Mgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 25, Mark A Magnuson | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Ins-Cre; Ins2cre; RIP Cre; RIP2-cre; RIPCre; Rip-cre; [RIP]-Cre; | ||
| Strain of Origin | (C57BL/6 x DBA)F2 | ||
| Site of Expression | pancreatic beta cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ins2, insulin 2, rat | ||
| Driver Note | Ins2 | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the rat insulin promoter (Ins2), which is active in pancreatic beta cells and in neurons of the hypothalamus. 9 copies of the transgene are estimated to be present. [MGI Ref ID J:51826] | ||
| Gene Symbol and Name | Tg(Ins2-cre)25Mgn, transgene insertion 25, Mark A Magnuson | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Ins-Cre; Ins2cre; RIP Cre; RIP2-cre; Rip-cre; [RIP]-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Postic C; Shiota M; Niswender KD; Jetton TL; Chen Y; Moates JM; Shelton KD; Lindner J; Cherrington AD; Magnuson MA. 1999. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J Biol Chem 274(1):305-15. [PubMed: 9867845] [MGI Ref ID J:51826]
Additional References
Crabtree JS; Scacheri PC; Ward JM; McNally SR; Swain GP; Montagna C; Hager JH; Hanahan D; Edlund H; Magnuson MA; Garrett-Beal L; Burns AL; Ried T; Chandrasekharappa SC; Marx SJ; Spiegel AM; Collins FS. 2003. Of mice and MEN1: Insulinomas in a conditional mouse knockout. Mol Cell Biol 23(17):6075-85. [PubMed: 12917331] [MGI Ref ID J:85042]
Gannon M; Shiota C; Postic C; Wright CV; Magnuson M. 2000. Analysis of the Cre-mediated recombination driven by rat insulin promoter in embryonic and adult mouse pancreas. Genesis 26(2):139-42. [PubMed: 10686610] [MGI Ref ID J:69314]
Gorogawa S; Fujitani Y; Kaneto H; Hazama Y; Watada H; Miyamoto Y; Takeda K; Akira S; Magnuson MA; Yamasaki Y; Kajimoto Y; Hori M. 2004. Insulin secretory defects and impaired islet architecture in pancreatic beta-cell-specific STAT3 knockout mice. Biochem Biophys Res Commun 319(4):1159-70. [PubMed: 15194489] [MGI Ref ID J:90753]
Martin J; Hunt SL; Dubus P; Sotillo R; Nehme-Pelluard F; Magnuson MA; Parlow AF; Malumbres M; Ortega S; Barbacid M. 2003. Genetic rescue of Cdk4 null mice restores pancreatic beta-cell proliferation but not homeostatic cell number. Oncogene 22(34):5261-9. [PubMed: 12917627] [MGI Ref ID J:85130]
Rosen ED; Kulkarni RN; Sarraf P; Ozcan U; Okada T; Hsu CH; Eisenman D; Magnuson MA; Gonzalez FJ; Kahn CR; Spiegelman BM. 2003. Targeted elimination of peroxisome proliferator-activated receptor gamma in beta cells leads to abnormalities in islet mass without compromising glucose homeostasis. Mol Cell Biol 23(20):7222-9. [PubMed: 14517292] [MGI Ref ID J:89959]
Schulla V; Renstrom E; Feil R; Feil S; Franklin I; Gjinovci A; Jing XJ; Laux D; Lundquist I; Magnuson MA; Obermuller S; Olofsson CS; Salehi A; Wendt A; Klugbauer N; Wollheim CB; Rorsman P; Hofmann F. 2003. Impaired insulin secretion and glucose tolerance in beta cell-selective Ca(v)1.2 Ca2+ channel null mice. EMBO J 22(15):3844-54. [PubMed: 12881419] [MGI Ref ID J:84919]
Tg(Ins2-cre)25Mgn relatedApostolou I; Hao Z; Rajewsky K; von Boehmer H. 2003. Effective destruction of Fas-deficient insulin-producing beta cells in type 1 diabetes. J Exp Med 198(7):1103-6. [PubMed: 14530378] [MGI Ref ID J:85985]
Atouf F; Park CH; Pechhold K; Ta M; Choi Y; Lumelsky NL. 2007. No evidence for mouse pancreatic beta-cell epithelial-mesenchymal transition in vitro. Diabetes 56(3):699-702. [PubMed: 17327438] [MGI Ref ID J:122025]
Bardoux P; Zhang P; Flamez D; Perilhou A; Lavin TA; Tanti JF; Hellemans K; Gomas E; Godard C; Andreelli F; Buccheri MA; Kahn A; Le Marchand-Brustel Y; Burcelin R; Schuit F; Vasseur-Cognet M. 2005. Essential Role of Chicken Ovalbumin Upstream Promoter-Transcription Factor II in Insulin Secretion and Insulin Sensitivity Revealed by Conditional Gene Knockout. Diabetes 54(5):1357-1363. [PubMed: 15855320] [MGI Ref ID J:98195]
Beall C; Piipari K; Al-Qassab H; Smith MA; Parker N; Carling D; Viollet B; Withers DJ; Ashford ML. 2010. Loss of AMP-activated protein kinase alpha2 subunit in mouse beta-cells impairs glucose-stimulated insulin secretion and inhibits their sensitivity to hypoglycaemia. Biochem J 429(2):323-33. [PubMed: 20465544] [MGI Ref ID J:164464]
Biondi CA; Gartside MG; Waring P; Loffler KA; Stark MS; Magnuson MA; Kay GF; Hayward NK. 2004. Conditional inactivation of the MEN1 gene leads to pancreatic and pituitary tumorigenesis but does not affect normal development of these tissues. Mol Cell Biol 24(8):3125-31. [PubMed: 15060136] [MGI Ref ID J:89898]
Brissova M; Shostak A; Shiota M; Wiebe PO; Poffenberger G; Kantz J; Chen Z; Carr C; Jerome WG; Chen J; Baldwin HS; Nicholson W; Bader DM; Jetton T; Gannon M; Powers AC. 2006. Pancreatic islet production of vascular endothelial growth factor--a is essential for islet vascularization, revascularization, and function. Diabetes 55(11):2974-85. [PubMed: 17065333] [MGI Ref ID J:116552]
Brunham LR; Kruit JK; Pape TD; Timmins JM; Reuwer AQ; Vasanji Z; Marsh BJ; Rodrigues B; Johnson JD; Parks JS; Verchere CB; Hayden MR. 2007. Beta-cell ABCA1 influences insulin secretion, glucose homeostasis and response to thiazolidinedione treatment. Nat Med 13(3):340-7. [PubMed: 17322896] [MGI Ref ID J:121696]
Cantley J; Choudhury AI; Asare-Anane H; Selman C; Lingard S; Heffron H; Herrera P; Persaud SJ; Withers DJ. 2007. Pancreatic deletion of insulin receptor substrate 2 reduces beta and alpha cell mass and impairs glucose homeostasis in mice. Diabetologia 50(6):1248-56. [PubMed: 17393136] [MGI Ref ID J:141585]
Cantley J; Selman C; Shukla D; Abramov AY; Forstreuter F; Esteban MA; Claret M; Lingard SJ; Clements M; Harten SK; Asare-Anane H; Batterham RL; Herrera PL; Persaud SJ; Duchen MR; Maxwell PH; Withers DJ. 2009. Deletion of the von Hippel-Lindau gene in pancreatic beta cells impairs glucose homeostasis in mice. J Clin Invest 119(1):125-35. [PubMed: 19065050] [MGI Ref ID J:144713]
Carrington EM; McKenzie MD; Jansen E; Myers M; Fynch S; Kos C; Strasser A; Kay TW; Scott CL; Allison J. 2009. Islet beta-cells deficient in Bcl-xL develop but are abnormally sensitive to apoptotic stimuli. Diabetes 58(10):2316-23. [PubMed: 19581414] [MGI Ref ID J:154403]
Casanovas O; Hager JH; Chun MG; Hanahan D. 2005. Incomplete inhibition of the Rb tumor suppressor pathway in the context of inactivated p53 is sufficient for pancreatic islet tumorigenesis. Oncogene 24(44):6597-604. [PubMed: 16007165] [MGI Ref ID J:101759]
Chakravarthy MV; Zhu Y; Lopez M; Yin L; Wozniak DF; Coleman T; Hu Z; Wolfgang M; Vidal-Puig A; Lane MD; Semenkovich CF. 2007. Brain fatty acid synthase activates PPARalpha to maintain energy homeostasis. J Clin Invest 117(9):2539-52. [PubMed: 17694178] [MGI Ref ID J:127482]
Chakravarthy MV; Zhu Y; Yin L; Coleman T; Pappan KL; Marshall CA; McDaniel ML; Semenkovich CF. 2009. Inactivation of hypothalamic FAS protects mice from diet-induced obesity and inflammation. J Lipid Res 50(4):630-40. [PubMed: 19029118] [MGI Ref ID J:158085]
Chase LG; Ulloa-Montoya F; Kidder BL; Verfaillie CM. 2007. Islet-derived fibroblast-like cells are not derived via epithelial-mesenchymal transition from Pdx-1 or insulin-positive cells. Diabetes 56(1):3-7. [PubMed: 17110468] [MGI Ref ID J:121938]
Chen J; Fontes G; Saxena G; Poitout V; Shalev A. 2010. Lack of TXNIP protects against mitochondria-mediated apoptosis but not against fatty acid-induced ER stress-mediated beta-cell death. Diabetes 59(2):440-7. [PubMed: 19875615] [MGI Ref ID J:164164]
Chen J; Hui ST; Couto FM; Mungrue IN; Davis DB; Attie AD; Lusis AJ; Davis RA; Shalev A. 2008. Thioredoxin-interacting protein deficiency induces Akt/Bcl-xL signaling and pancreatic beta-cell mass and protects against diabetes. FASEB J 22(10):3581-94. [PubMed: 18552236] [MGI Ref ID J:140264]
Choi D; Cai EP; Schroer SA; Wang L; Woo M. 2011. Vhl is required for normal pancreatic beta cell function and the maintenance of beta cell mass with age in mice. Lab Invest 91(4):527-38. [PubMed: 21242957] [MGI Ref ID J:170625]
Choi D; Nguyen KT; Wang L; Schroer SA; Suzuki A; Mak TW; Woo M. 2008. Partial deletion of Pten in the hypothalamus leads to growth defects that cannot be rescued by exogenous growth hormone. Endocrinology 149(9):4382-6. [PubMed: 18499759] [MGI Ref ID J:145502]
Choi D; Schroer SA; Lu SY; Wang L; Wu X; Liu Y; Zhang Y; Gaisano HY; Wagner KU; Wu H; Retnakaran R; Woo M. 2010. Erythropoietin protects against diabetes through direct effects on pancreatic beta cells. J Exp Med 207(13):2831-42. [PubMed: 21149549] [MGI Ref ID J:176862]
Choudhury AI; Heffron H; Smith MA; Al-Qassab H; Xu AW; Selman C; Simmgen M; Clements M; Claret M; Maccoll G; Bedford DC; Hisadome K; Diakonov I; Moosajee V; Bell JD; Speakman JR; Batterham RL; Barsh GS; Ashford ML; Withers DJ. 2005. The role of insulin receptor substrate 2 in hypothalamic and beta cell function. J Clin Invest 115(4):940-950. [PubMed: 15841180] [MGI Ref ID J:97406]
Covey SD; Wideman RD; McDonald C; Unniappan S; Huynh F; Asadi A; Speck M; Webber T; Chua SC; Kieffer TJ. 2006. The pancreatic beta cell is a key site for mediating the effects of leptin on glucose homeostasis. Cell Metab 4(4):291-302. [PubMed: 17011502] [MGI Ref ID J:129737]
Crabtree JS; Scacheri PC; Ward JM; McNally SR; Swain GP; Montagna C; Hager JH; Hanahan D; Edlund H; Magnuson MA; Garrett-Beal L; Burns AL; Ried T; Chandrasekharappa SC; Marx SJ; Spiegel AM; Collins FS. 2003. Of mice and MEN1: Insulinomas in a conditional mouse knockout. Mol Cell Biol 23(17):6075-85. [PubMed: 12917331] [MGI Ref ID J:85042]
Cui J; Wang Z; Cheng Q; Lin R; Zhang XM; Leung PS; Copeland NG; Jenkins NA; Yao KM; Huang JD. 2011. Targeted inactivation of kinesin-1 in pancreatic beta-cells in vivo leads to insulin secretory deficiency. Diabetes 60(1):320-30. [PubMed: 20870970] [MGI Ref ID J:170198]
Cui Y; Huang L; Elefteriou F; Yang G; Shelton JM; Giles JE; Oz OK; Pourbahrami T; Lu CY; Richardson JA; Karsenty G; Li C. 2004. Essential role of STAT3 in body weight and glucose homeostasis. Mol Cell Biol 24(1):258-69. [PubMed: 14673160] [MGI Ref ID J:87596]
Dabernat S; Secrest P; Peuchant E; Moreau-Gaudry F; Dubus P; Sarvetnick N. 2009. Lack of beta-catenin in early life induces abnormal glucose homeostasis in mice. Diabetologia 52(8):1608-17. [PubMed: 19513688] [MGI Ref ID J:150423]
Dai C; Huh CG; Thorgeirsson SS; Liu Y. 2005. {beta}-Cell-Specific Ablation of the Hepatocyte Growth Factor Receptor Results in Reduced Islet Size, Impaired Insulin Secretion, and Glucose Intolerance. Am J Pathol 167(2):429-36. [PubMed: 16049329] [MGI Ref ID J:99948]
Dieguez-Hurtado R; Martin J; Martinez-Corral I; Martinez MD; Megias D; Olmeda D; Ortega S. 2011. A Cre-reporter transgenic mouse expressing the far-red fluorescent protein Katushka. Genesis 49(1):36-45. [PubMed: 21254335] [MGI Ref ID J:167974]
Ebato C; Uchida T; Arakawa M; Komatsu M; Ueno T; Komiya K; Azuma K; Hirose T; Tanaka K; Kominami E; Kawamori R; Fujitani Y; Watada H. 2008. Autophagy is important in islet homeostasis and compensatory increase of beta cell mass in response to high-fat diet. Cell Metab 8(4):325-32. [PubMed: 18840363] [MGI Ref ID J:143423]
Fan Y; Rudert WA; Grupillo M; He J; Sisino G; Trucco M. 2009. Thymus-specific deletion of insulin induces autoimmune diabetes. EMBO J 28(18):2812-24. [PubMed: 19680229] [MGI Ref ID J:152798]
Fex M; Haemmerle G; Wierup N; Dekker-Nitert M; Rehn M; Ristow M; Zechner R; Sundler F; Holm C; Eliasson L; Mulder H. 2009. A beta cell-specific knockout of hormone-sensitive lipase in mice results in hyperglycaemia and disruption of exocytosis. Diabetologia 52(2):271-80. [PubMed: 19023560] [MGI Ref ID J:144744]
Fex M; Wierup N; Nitert MD; Ristow M; Mulder H. 2007. Rat insulin promoter 2-Cre recombinase mice bred onto a pure C57BL/6J background exhibit unaltered glucose tolerance. J Endocrinol 194(3):551-5. [PubMed: 17761894] [MGI Ref ID J:145236]
Fujioka M; Tokano H; Fujioka KS; Okano H; Edge AS. 2011. Generating mouse models of degenerative diseases using Cre/lox-mediated in vivo mosaic cell ablation. J Clin Invest 121(6):2462-9. [PubMed: 21576819] [MGI Ref ID J:173515]
Gannon M; Ables ET; Crawford L; Lowe D; Offield MF; Magnuson MA; Wright CV. 2008. pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis. Dev Biol 314(2):406-17. [PubMed: 18155690] [MGI Ref ID J:130389]
Gannon M; Shiota C; Postic C; Wright CV; Magnuson M. 2000. Analysis of the Cre-mediated recombination driven by rat insulin promoter in embryonic and adult mouse pancreas. Genesis 26(2):139-42. [PubMed: 10686610] [MGI Ref ID J:69314]
Gautam D; Han SJ; Hamdan FF; Jeon J; Li B; Li JH; Cui Y; Mears D; Lu H; Deng C; Heard T; Wess J. 2006. A critical role for beta cell M3 muscarinic acetylcholine receptors in regulating insulin release and blood glucose homeostasis in vivo. Cell Metab 3(6):449-61. [PubMed: 16753580] [MGI Ref ID J:129643]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Gorogawa S; Fujitani Y; Kaneto H; Hazama Y; Watada H; Miyamoto Y; Takeda K; Akira S; Magnuson MA; Yamasaki Y; Kajimoto Y; Hori M. 2004. Insulin secretory defects and impaired islet architecture in pancreatic beta-cell-specific STAT3 knockout mice. Biochem Biophys Res Commun 319(4):1159-70. [PubMed: 15194489] [MGI Ref ID J:90753]
Gu Y; Lindner J; Kumar A; Yuan W; Magnuson MA. 2011. Rictor/mTORC2 is essential for maintaining a balance between beta-cell proliferation and cell size. Diabetes 60(3):827-37. [PubMed: 21266327] [MGI Ref ID J:170129]
Gupta RK; Gao N; Gorski RK; White P; Hardy OT; Rafiq K; Brestelli JE; Chen G; Stoeckert CJ Jr; Kaestner KH. 2007. Expansion of adult beta-cell mass in response to increased metabolic demand is dependent on HNF-4alpha. Genes Dev 21(7):756-69. [PubMed: 17403778] [MGI Ref ID J:120377]
Gupta RK; Vatamaniuk MZ; Lee CS; Flaschen RC; Fulmer JT; Matschinsky FM; Duncan SA; Kaestner KH. 2005. The MODY1 gene HNF-4alpha regulates selected genes involved in insulin secretion. J Clin Invest 115(4):1006-1015. [PubMed: 15761495] [MGI Ref ID J:97364]
Hager JH; Ulanet DB; Hennighausen L; Hanahan D. 2009. Genetic ablation of Bcl-x attenuates invasiveness without affecting apoptosis or tumor growth in a mouse model of pancreatic neuroendocrine cancer. PLoS ONE 4(2):e4455. [PubMed: 19209227] [MGI Ref ID J:146436]
Harb G; Vasavada RC; Cobrinik D; Stewart AF. 2009. The retinoblastoma protein and its homolog p130 regulate the G1/S transition in pancreatic beta-cells. Diabetes 58(8):1852-62. [PubMed: 19509021] [MGI Ref ID J:154413]
Hashimoto N; Kido Y; Uchida T; Matsuda T; Suzuki K; Inoue H; Matsumoto M; Ogawa W; Maeda S; Fujihara H; Ueta Y; Uchiyama Y; Akimoto K; Ohno S; Noda T; Kasuga M. 2005. PKClambda regulates glucose-induced insulin secretion through modulation of gene expression in pancreatic beta cells. J Clin Invest 115(1):138-45. [PubMed: 15630453] [MGI Ref ID J:95378]
Hisadome K; Smith MA; Choudhury AI; Claret M; Withers DJ; Ashford ML. 2009. 5-HT inhibition of rat insulin 2 promoter Cre recombinase transgene and proopiomelanocortin neuron excitability in the mouse arcuate nucleus. Neuroscience 159(1):83-93. [PubMed: 19135134] [MGI Ref ID J:148970]
Inoue M; Hager JH; Ferrara N; Gerber HP; Hanahan D. 2002. VEGF-A has a critical, nonredundant role in angiogenic switching and pancreatic beta cell carcinogenesis. Cancer Cell 1(2):193-202. [PubMed: 12086877] [MGI Ref ID J:77131]
Jung HS; Chung KW; Won Kim J; Kim J; Komatsu M; Tanaka K; Nguyen YH; Kang TM; Yoon KH; Kim JW; Jeong YT; Han MS; Lee MK; Kim KW; Shin J; Lee MS. 2008. Loss of autophagy diminishes pancreatic beta cell mass and function with resultant hyperglycemia. Cell Metab 8(4):318-24. [PubMed: 18840362] [MGI Ref ID J:143424]
Kaneko K; Ueki K; Takahashi N; Hashimoto S; Okamoto M; Awazawa M; Okazaki Y; Ohsugi M; Inabe K; Umehara T; Yoshida M; Kakei M; Kitamura T; Luo J; Kulkarni RN; Kahn CR; Kasai H; Cantley LC; Kadowaki T. 2010. Class IA phosphatidylinositol 3-kinase in pancreatic beta cells controls insulin secretion by multiple mechanisms. Cell Metab 12(6):619-32. [PubMed: 21109194] [MGI Ref ID J:168115]
Kruit JK; Kremer PH; Dai L; Tang R; Ruddle P; de Haan W; Brunham LR; Verchere CB; Hayden MR. 2010. Cholesterol efflux via ATP-binding cassette transporter A1 (ABCA1) and cholesterol uptake via the LDL receptor influences cholesterol-induced impairment of beta cell function in mice. Diabetologia 53(6):1110-9. [PubMed: 20229095] [MGI Ref ID J:159879]
Kubota N; Terauchi Y; Tobe K; Yano W; Suzuki R; Ueki K; Takamoto I; Satoh H; Maki T; Kubota T; Moroi M; Okada-Iwabu M; Ezaki O; Nagai R; Ueta Y; Kadowaki T; Noda T. 2004. Insulin receptor substrate 2 plays a crucial role in beta cells and the hypothalamus. J Clin Invest 114(7):917-27. [PubMed: 15467830] [MGI Ref ID J:93416]
Kulkarni RN; Bruning JC; Winnay JN; Postic C; Magnuson MA; Kahn CR. 1999. Tissue-specific knockout of the insulin receptor in pancreatic beta cells creates an insulin secretory defect similar to that in type 2 diabetes. Cell 96(3):329-39. [PubMed: 10025399] [MGI Ref ID J:67938]
Kulkarni RN; Holzenberger M; Shih DQ; Ozcan U; Stoffel M; Magnuson MA; Kahn CR. 2002. beta-cell-specific deletion of the Igf1 receptor leads to hyperinsulinemia and glucose intolerance but does not alter beta-cell mass. Nat Genet 31(1):111-5. [PubMed: 11923875] [MGI Ref ID J:76208]
Lantz KA; Vatamaniuk MZ; Brestelli JE; Friedman JR; Matschinsky FM; Kaestner KH. 2004. Foxa2 regulates multiple pathways of insulin secretion. J Clin Invest 114(4):512-20. [PubMed: 15314688] [MGI Ref ID J:91995]
Lee AH; Heidtman K; Hotamisligil GS; Glimcher LH. 2011. Dual and opposing roles of the unfolded protein response regulated by IRE1{alpha} and XBP1 in proinsulin processing and insulin secretion. Proc Natl Acad Sci U S A 108(21):8885-90. [PubMed: 21555585] [MGI Ref ID J:171889]
Lee CS; Sund NJ; Vatamaniuk MZ; Matschinsky FM; Stoffers DA; Kaestner KH. 2002. Foxa2 controls Pdx1 gene expression in pancreatic beta-cells in vivo. Diabetes 51(8):2546-51. [PubMed: 12145169] [MGI Ref ID J:78086]
Lee JY; Gavrilova O; Davani B; Na R; Robinson GW; Hennighausen L. 2007. The transcription factors Stat5a/b are not required for islet development but modulate pancreatic beta-cell physiology upon aging. Biochim Biophys Acta 1773(9):1455-61. [PubMed: 17599554] [MGI Ref ID J:127051]
Lee JY; Ristow M; Lin X; White MF; Magnuson MA; Hennighausen L. 2006. RIP-Cre revisited, evidence for impairments of pancreatic beta-cell function. J Biol Chem 281(5):2649-53. [PubMed: 16326700] [MGI Ref ID J:108244]
Leiss V; Friebe A; Welling A; Hofmann F; Lukowski R. 2011. Cyclic GMP kinase I modulates glucagon release from pancreatic alpha-cells. Diabetes 60(1):148-56. [PubMed: 20978093] [MGI Ref ID J:170155]
Liadis N; Salmena L; Kwan E; Tajmir P; Schroer SA; Radziszewska A; Li X; Sheu L; Eweida M; Xu S; Gaisano HY; Hakem R; Woo M. 2007. Distinct in vivo roles of caspase-8 in beta-cells in physiological and diabetes models. Diabetes 56(9):2302-11. [PubMed: 17563067] [MGI Ref ID J:126586]
Lin WY; Hu YJ; Lee YH. 2008. Hepatocyte nuclear factor-1alpha regulates glucocorticoid receptor expression to control postnatal body growth. Am J Physiol Gastrointest Liver Physiol 295(3):G542-51. [PubMed: 18583462] [MGI Ref ID J:141912]
Lin X; Taguchi A; Park S; Kushner JA; Li F; Li Y; White MF. 2004. Dysregulation of insulin receptor substrate 2 in beta cells and brain causes obesity and diabetes. J Clin Invest 114(7):908-16. [PubMed: 15467829] [MGI Ref ID J:93415]
Liu S; Okada T; Assmann A; Soto J; Liew CW; Bugger H; Shirihai OS; Abel ED; Kulkarni RN. 2009. Insulin signaling regulates mitochondrial function in pancreatic beta-cells. PLoS One 4(11):e7983. [PubMed: 19956695] [MGI Ref ID J:155382]
Martin J; Hunt SL; Dubus P; Sotillo R; Nehme-Pelluard F; Magnuson MA; Parlow AF; Malumbres M; Ortega S; Barbacid M. 2003. Genetic rescue of Cdk4 null mice restores pancreatic beta-cell proliferation but not homeostatic cell number. Oncogene 22(34):5261-9. [PubMed: 12917627] [MGI Ref ID J:85130]
Mauvais-Jarvis F; Virkamaki A; Michael MD; Winnay JN; Zisman A; Kulkarni RN; Kahn CR. 2000. A model to explore the interaction between muscle insulin resistance and beta-cell dysfunction in the development of type 2 diabetes. Diabetes 49(12):2126-34. [PubMed: 11118016] [MGI Ref ID J:66009]
Miura A; Yamagata K; Kakei M; Hatakeyama H; Takahashi N; Fukui K; Nammo T; Yoneda K; Inoue Y; Sladek FM; Magnuson MA; Kasai H; Miyagawa J; Gonzalez FJ; Shimomura I. 2006. Hepatocyte nuclear factor-4alpha is essential for glucose-stimulated insulin secretion by pancreatic beta-cells. J Biol Chem 281(8):5246-57. [PubMed: 16377800] [MGI Ref ID J:108652]
Mori H; Inoki K; Munzberg H; Opland D; Faouzi M; Villanueva EC; Ikenoue T; Kwiatkowski D; MacDougald OA; Myers MG Jr; Guan KL. 2009. Critical role for hypothalamic mTOR activity in energy balance. Cell Metab 9(4):362-74. [PubMed: 19356717] [MGI Ref ID J:148167]
Mori H; Inoki K; Opland D; Muenzberg H; Villanueva EC; Faouzi M; Ikenoue T; Kwiatkowski D; Macdougald OA; Myers Jr MG; Guan KL. 2009. Critical roles for the TSC-mTOR pathway in {beta}-cell function. Am J Physiol Endocrinol Metab :. [PubMed: 19690069] [MGI Ref ID J:159576]
Mori H; Shichita T; Yu Q; Yoshida R; Hashimoto M; Okamoto F; Torisu T; Nakaya M; Kobayashi T; Takaesu G; Yoshimura A. 2007. Suppression of SOCS3 expression in the pancreatic beta-cell leads to resistance to type 1 diabetes. Biochem Biophys Res Commun 359(4):952-8. [PubMed: 17562326] [MGI Ref ID J:122729]
Nguyen KT; Tajmir P; Lin CH; Liadis N; Zhu XD; Eweida M; Tolasa-Karaman G; Cai F; Wang R; Kitamura T; Belsham DD; Wheeler MB; Suzuki A; Mak TW; Woo M. 2006. Essential role of Pten in body size determination and pancreatic beta-cell homeostasis in vivo. Mol Cell Biol 26(12):4511-8. [PubMed: 16738317] [MGI Ref ID J:109610]
Okada T; Liew CW; Hu J; Hinault C; Michael MD; Krtzfeldt J; Yin C; Holzenberger M; Stoffel M; Kulkarni RN. 2007. From the Cover: Insulin receptors in beta-cells are critical for islet compensatory growth response to insulin resistance. Proc Natl Acad Sci U S A 104(21):8977-82. [PubMed: 17416680] [MGI Ref ID J:121640]
Otani K; Kulkarni RN; Baldwin AC; Krutzfeldt J; Ueki K; Stoffel M; Kahn CR; Polonsky KS. 2004. Reduced beta-cell mass and altered glucose sensing impair insulin-secretory function in betaIRKO mice. Am J Physiol Endocrinol Metab 286(1):E41-9. [PubMed: 14519599] [MGI Ref ID J:87825]
Pappan KL; Pan Z; Kwon G; Marshall CA; Coleman T; Goldberg IJ; McDaniel ML; Semenkovich CF. 2005. Pancreatic beta-cell lipoprotein lipase independently regulates islet glucose metabolism and normal insulin secretion. J Biol Chem 280(10):9023-9. [PubMed: 15637076] [MGI Ref ID J:97791]
Pigeau GM; Kolic J; Ball BJ; Hoppa MB; Wang YW; Ruckle T; Woo M; Manning Fox JE; MacDonald PE. 2009. Insulin granule recruitment and exocytosis is dependent on p110gamma in insulinoma and human beta-cells. Diabetes 58(9):2084-92. [PubMed: 19549714] [MGI Ref ID J:154404]
Pomplun D; Florian S; Schulz T; Pfeiffer AF; Ristow M. 2007. Alterations of pancreatic beta-cell mass and islet number due to Ins2-controlled expression of Cre recombinase: RIP-Cre revisited; part 2. Horm Metab Res 39(5):336-40. [PubMed: 17533574] [MGI Ref ID J:127221]
Radziszewska A; Choi D; Nguyen KT; Schroer SA; Tajmir P; Wang L; Suzuki A; Mak TW; Evan GI; Woo M. 2009. PTEN deletion and concomitant c-Myc activation do not lead to tumor formation in pancreatic beta cells. J Biol Chem 284(5):2917-22. [PubMed: 19056726] [MGI Ref ID J:147214]
Regard JB; Kataoka H; Cano DA; Camerer E; Yin L; Zheng YW; Scanlan TS; Hebrok M; Coughlin SR. 2007. Probing cell type-specific functions of Gi in vivo identifies GPCR regulators of insulin secretion. J Clin Invest 117(12):4034-43. [PubMed: 17992256] [MGI Ref ID J:130781]
Ristow M; Mulder H; Pomplun D; Schulz TJ; Muller-Schmehl K; Krause A; Fex M; Puccio H; Muller J; Isken F; Spranger J; Muller-Wieland D; Magnuson MA; Mohlig M; Koenig M; Pfeiffer AF. 2003. Frataxin deficiency in pancreatic islets causes diabetes due to loss of beta cell mass. J Clin Invest 112(4):527-34. [PubMed: 12925693] [MGI Ref ID J:85821]
Roccisana J; Reddy V; Vasavada RC; Gonzalez-Pertusa JA; Magnuson MA; Garcia-Ocana A. 2005. Targeted inactivation of hepatocyte growth factor receptor c-met in beta-cells leads to defective insulin secretion and GLUT-2 downregulation without alteration of beta-cell mass. Diabetes 54(7):2090-102. [PubMed: 15983210] [MGI Ref ID J:100092]
Rosen ED; Kulkarni RN; Sarraf P; Ozcan U; Okada T; Hsu CH; Eisenman D; Magnuson MA; Gonzalez FJ; Kahn CR; Spiegelman BM. 2003. Targeted elimination of peroxisome proliferator-activated receptor gamma in beta cells leads to abnormalities in islet mass without compromising glucose homeostasis. Mol Cell Biol 23(20):7222-9. [PubMed: 14517292] [MGI Ref ID J:89959]
Sassmann A; Gier B; Grone HJ; Drews G; Offermanns S; Wettschureck N. 2010. The Gq/G11-mediated signaling pathway is critical for autocrine potentiation of insulin secretion in mice. J Clin Invest 120(6):2184-93. [PubMed: 20440069] [MGI Ref ID J:161458]
Schulla V; Renstrom E; Feil R; Feil S; Franklin I; Gjinovci A; Jing XJ; Laux D; Lundquist I; Magnuson MA; Obermuller S; Olofsson CS; Salehi A; Wendt A; Klugbauer N; Wollheim CB; Rorsman P; Hofmann F. 2003. Impaired insulin secretion and glucose tolerance in beta cell-selective Ca(v)1.2 Ca2+ channel null mice. EMBO J 22(15):3844-54. [PubMed: 12881419] [MGI Ref ID J:84919]
Schumann DM; Maedler K; Franklin I; Konrad D; Storling J; Boni-Schnetzler M; Gjinovci A; Kurrer MO; Gauthier BR; Bosco D; Andres A; Berney T; Greter M; Becher B; Chervonsky AV; Halban PA; Mandrup-Poulsen T; Wollheim CB; Donath MY. 2007. The Fas pathway is involved in pancreatic beta cell secretory function. Proc Natl Acad Sci U S A 104(8):2861-6. [PubMed: 17299038] [MGI Ref ID J:125892]
Shen HC; Adem A; Ylaya K; Wilson A; He M; Lorang D; Hewitt SM; Pechhold K; Harlan DM; Lubensky IA; Schmidt LS; Linehan WM; Libutti SK. 2009. Deciphering von Hippel-Lindau (VHL/Vhl)-associated pancreatic manifestations by inactivating Vhl in specific pancreatic cell populations. PLoS ONE 4(4):e4897. [PubMed: 19340311] [MGI Ref ID J:148174]
Shigeyama Y; Kobayashi T; Kido Y; Hashimoto N; Asahara S; Matsuda T; Takeda A; Inoue T; Shibutani Y; Koyanagi M; Uchida T; Inoue M; Hino O; Kasuga M; Noda T. 2008. Biphasic response of pancreatic beta-cell mass to ablation of tuberous sclerosis complex 2 in mice. Mol Cell Biol 28(9):2971-9. [PubMed: 18316403] [MGI Ref ID J:135811]
Silva JP; Kohler M; Graff C; Oldfors A; Magnuson MA; Berggren PO; Larsson NG. 2000. Impaired insulin secretion and beta-cell loss in tissue-specific knockout mice with mitochondrial diabetes Nat Genet 26(3):336-40. [PubMed: 11062475] [MGI Ref ID J:65522]
Song J; Xu Y; Hu X; Choi B; Tong Q. 2010. Brain expression of Cre recombinase driven by pancreas-specific promoters. Genesis 48(11):628-34. [PubMed: 20824628] [MGI Ref ID J:166612]
Song WJ; Seshadri M; Ashraf U; Mdluli T; Mondal P; Keil M; Azevedo M; Kirschner LS; Stratakis CA; Hussain MA. 2011. Snapin mediates incretin action and augments glucose-dependent insulin secretion. Cell Metab 13(3):308-19. [PubMed: 21356520] [MGI Ref ID J:172258]
Srinivasan M; Choi CS; Ghoshal P; Pliss L; Pandya JD; Hill D; Cline G; Patel MS. 2010. ss-Cell-specific pyruvate dehydrogenase deficiency impairs glucose-stimulated insulin secretion. Am J Physiol Endocrinol Metab 299(6):E910-7. [PubMed: 20841503] [MGI Ref ID J:170211]
Stiles BL; Kuralwalla-Martinez C; Guo W; Gregorian C; Wang Y; Tian J; Magnuson MA; Wu H. 2006. Selective deletion of Pten in pancreatic beta cells leads to increased islet mass and resistance to STZ-induced diabetes. Mol Cell Biol 26(7):2772-81. [PubMed: 16537919] [MGI Ref ID J:106937]
Su H; Marcheva B; Meng S; Liang FA; Kohsaka A; Kobayashi Y; Xu AW; Bass J; Wang X. 2010. Gamma-protocadherins regulate the functional integrity of hypothalamic feeding circuitry in mice. Dev Biol 339(1):38-50. [PubMed: 20025866] [MGI Ref ID J:157958]
Sun G; Reynolds R; Leclerc I; Rutter GA. 2011. RIP2-mediated LKB1 deletion causes axon degeneration in the spinal cord and hind-limb paralysis. Dis Model Mech 4(2):193-202. [PubMed: 21135058] [MGI Ref ID J:169259]
Sun G; Tarasov AI; McGinty JA; French PM; McDonald A; Leclerc I; Rutter GA. 2010. LKB1 deletion with the RIP2.Cre transgene modifies pancreatic beta-cell morphology and enhances insulin secretion in vivo. Am J Physiol Endocrinol Metab 298(6):E1261-73. [PubMed: 20354156] [MGI Ref ID J:162875]
Sund NJ; Vatamaniuk MZ; Casey M; Ang SL; Magnuson MA; Stoffers DA; Matschinsky FM; Kaestner KH. 2001. Tissue-specific deletion of Foxa2 in pancreatic beta cells results in hyperinsulinemic hypoglycemia. Genes Dev 15(13):1706-15. [PubMed: 11445544] [MGI Ref ID J:70409]
Takahashi I; Noguchi N; Nata K; Yamada S; Kaneiwa T; Mizumoto S; Ikeda T; Sugihara K; Asano M; Yoshikawa T; Yamauchi A; Shervani NJ; Uruno A; Kato I; Unno M; Sugahara K; Takasawa S; Okamoto H; Sugawara A. 2009. Important role of heparan sulfate in postnatal islet growth and insulin secretion. Biochem Biophys Res Commun 383(1):113-8. [PubMed: 19336225] [MGI Ref ID J:148348]
Toyofuku Y; Uchida T; Nakayama S; Hirose T; Kawamori R; Fujitani Y; Inoue M; Watada H. 2009. Normal islet vascularization is dispensable for expansion of beta-cell mass in response to high-fat diet induced insulin resistance. Biochem Biophys Res Commun 383(3):303-7. [PubMed: 19336220] [MGI Ref ID J:149603]
Ulanet DB; Ludwig DL; Kahn CR; Hanahan D. 2010. Insulin receptor functionally enhances multistage tumor progression and conveys intrinsic resistance to IGF-1R targeted therapy. Proc Natl Acad Sci U S A 107(24):10791-8. [PubMed: 20457905] [MGI Ref ID J:165059]
Vasavada RC; Cozar-Castellano I; Sipula D; Stewart AF. 2007. Tissue-specific deletion of the retinoblastoma protein in the pancreatic beta-cell has limited effects on beta-cell replication, mass, and function. Diabetes 56(1):57-64. [PubMed: 17192465] [MGI Ref ID J:121935]
Wang L; Coffinier C; Thomas MK; Gresh L; Eddu G; Manor T; Levitsky LL; Yaniv M; Rhoads DB. 2004. Selective deletion of the Hnf1beta (MODY5) gene in beta-cells leads to altered gene expression and defective insulin release. Endocrinology 145(8):3941-9. [PubMed: 15142986] [MGI Ref ID J:91534]
Wang L; Liu Y; Yan Lu S; Nguyen KT; Schroer SA; Suzuki A; Mak TW; Gaisano H; Woo M. 2010. Deletion of Pten in pancreatic ss-cells protects against deficient ss-cell mass and function in mouse models of type 2 diabetes. Diabetes 59(12):3117-26. [PubMed: 20852026] [MGI Ref ID J:170206]
Wang S; Jensen JN; Seymour PA; Hsu W; Dor Y; Sander M; Magnuson MA; Serup P; Gu G. 2009. Sustained Neurog3 expression in hormone-expressing islet cells is required for endocrine maturation and function. Proc Natl Acad Sci U S A 106(24):9715-20. [PubMed: 19487660] [MGI Ref ID J:150074]
Wei FY; Suzuki T; Watanabe S; Kimura S; Kaitsuka T; Fujimura A; Matsui H; Atta M; Michiue H; Fontecave M; Yamagata K; Suzuki T; Tomizawa K. 2011. Deficit of tRNA(Lys) modification by Cdkal1 causes the development of type 2 diabetes in mice. J Clin Invest 121(9):3598-608. [PubMed: 21841312] [MGI Ref ID J:178248]
Wicksteed B; Brissova M; Yan W; Opland DM; Plank JL; Reinert RB; Dickson LM; Tamarina NA; Philipson LH; Shostak A; Bernal-Mizrachi E; Elghazi L; Roe MW; Labosky PA; Myers MG Jr; Gannon M; Powers AC; Dempsey PJ. 2010. Conditional gene targeting in mouse pancreatic ss-Cells: analysis of ectopic Cre transgene expression in the brain. Diabetes 59(12):3090-8. [PubMed: 20802254] [MGI Ref ID J:169727]
Wijesekara N; Dai FF; Hardy AB; Giglou PR; Bhattacharjee A; Koshkin V; Chimienti F; Gaisano HY; Rutter GA; Wheeler MB. 2010. Beta cell-specific Znt8 deletion in mice causes marked defects in insulin processing, crystallisation and secretion. Diabetologia 53(8):1656-68. [PubMed: 20424817] [MGI Ref ID J:162163]
Wu Y; Liu C; Sun H; Vijayakumar A; Giglou PR; Qiao R; Oppenheimer J; Yakar S; LeRoith D. 2011. Growth hormone receptor regulates beta cell hyperplasia and glucose-stimulated insulin secretion in obese mice. J Clin Invest 121(6):2422-6. [PubMed: 21555853] [MGI Ref ID J:173909]
Xie L; Duncan MB; Pahler J; Sugimoto H; Martino M; Lively J; Mundel T; Soubasakos M; Rubin K; Takeda T; Inoue M; Lawler J; Hynes RO; Hanahan D; Kalluri R. 2011. Counterbalancing angiogenic regulatory factors control the rate of cancer progression and survival in a stage-specific manner. Proc Natl Acad Sci U S A 108(24):9939-44. [PubMed: 21622854] [MGI Ref ID J:173322]
Xie T; Chen M; Zhang QH; Ma Z; Weinstein LS. 2007. Beta cell-specific deficiency of the stimulatory G protein alpha-subunit Gsalpha leads to reduced beta cell mass and insulin-deficient diabetes. Proc Natl Acad Sci U S A 104(49):19601-6. [PubMed: 18029451] [MGI Ref ID J:128497]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice may be bred as homozygotes. Expected coat color from breeding is Black. Mating System Homozygote x Homozygote (Female x Male) 03-SEP-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $225.00 Female or Male Homozygous for Tg(Ins2-cre)25Mgn
Pairs /Price (US dollars $) Pair Genotype $450.00 Homozygous for Tg(Ins2-cre)25Mgn x Homozygous for Tg(Ins2-cre)25Mgn Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $292.50 Female or Male Homozygous for Tg(Ins2-cre)25Mgn
Pairs /Price (US dollars $) Pair Genotype $585.00 Homozygous for Tg(Ins2-cre)25Mgn x Homozygous for Tg(Ins2-cre)25Mgn Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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