Strain Name: |
B6.Cg-Tg(Alb-cre)21Mgn/J |
|---|---|
Stock Number: |
003574 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
C57BL/6-Tg(Alb-cre)21Mgn/J (Changed: 15-DEC-04
) |
|
C57BL/6-TgN(Alb-Cre)21Mgn (Changed: 15-DEC-04
) | |
|
C57BL/6-TgN(AlbCre)21Mgn (Changed: 15-DEC-04
) | |
| Genes & Alleles | Alb; cre; Tg(Alb-cre)21Mgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Mark Magnuson, Vanderbilt University School of Medicine Generation N7+1F15N1F0 (05-DEC-07) Strain Description
This strain may be maintained as a hemizygote or homozygote and lacks any phenotype related to the transgenic allele. It has been well characterized and shown to be very efficient for performing liver-specific gene knockouts using Cre/loxP system. This line has been shown to be nearly 100% efficient in achieving liver-specific recombination when crossed with at least 5 different floxed alleles.Strain Development
A transgenic construct containing a 2.34 kb mouse albumin enhancer/promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kb fragment from the human growth hormone gene was injected into B6D2(F2) pronuclei. These mice were subsequently backcrossed to C57BL/6J mice.
Gene & Allele Details
| Allele Symbol | Tg(Alb-cre)21Mgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 21, Mark A Magnuson | ||
| Common Name(s) | Alb-cre; Albcre; AlbCre; albumin-cre; crAlb; | ||
| Mutation Made By | Mark Magnuson, Vanderbilt University School of Medicine | ||
| Strain of Origin | (C57BL/6 x DBA)F2 | ||
| Site of Expression | liver | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Alb, albumin, rat | ||
| Molecular Note | The transgene contains a 2.34 kb mouse albumin enhancer/promoter, a nuclear localization sequence-modified Cr recombinase gene and a 2.1 kb fragment from the human growth hormone gene. The promoter is active in hepatocyte cells of the liver. [MGI Ref ID J:51826] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Generic Cre QPCR
Colony Maintenance
| Breeding & Husbandry | The strain originated on a B6D2(F2) background. The allele was subsequently moved to a C57BL/6J background. Expected coat color from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Alb
003489 C57BL/6-Tg(AlbLCAT)1Jdm/J View Strains carrying other alleles of Alb (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
JAX® NOTES, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: pancreatic beta cells)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesPostic C; Shiota M; Niswender KD; Jetton TL; Chen Y; Moates JM; Shelton KD; Lindner J; Cherrington AD; Magnuson MA. 1999. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic beta cell-specific gene knock-outs using Cre recombinase. J Biol Chem 274(1):305-15. [PubMed: 9867845] [MGI Ref ID J:51826]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Alb-cre)21Mgn/J |
| Stock Number: | 003574 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Tel: 800.422.6423 or 207.288.5845
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