Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain BALB/cByJ Donor Strain B6,129S4-Dab1tm1Cpr (129S4 derived AK7 ES cell line)   Donating Investigator Dr. Jonathan A. Cooper,   Fred Hutchinson Cancer Research Center

Description
Homozygous Dab1-null mice become tremulous and ataxic at approximately postnatal day 10. Multiple defects can be detected in brain tissue. Lamellar structures in the cortex and hippocampus appear disorganized and the cerebellum is small and disorganized. Although homozygotes null reportedly die at 4-5 weeks on a mixed B6,129 or 129/Sv background, mice on a BALB/cByJ are viable.

Control Information

	Control
	Wild-type from the colony
	001026 BALB/cByJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Dab1

006408	B6.Cg-Dab1scm-3J/J
010970	CBA/J-Dab1scm-4J/GrsrJ
003925	MRL(Cg)-Dab1scm-2J/J
002043	STOCK A/A-Dab1scm/J

View Strains carrying other alleles of Dab1     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Dab1^tm1Cpr/Dab1^tm1Cpr

        involves: 129/Sv * C57BL/6

• nervous system phenotype
• abnormal cerebral cortex morphology
  • the cortical layers are inverted   (MGI Ref ID J:91679)

Dab1^tm1Cpr/Dab1^tm1Cpr

        involves: 129S4/SvJaeSor * C57BL/6

• mortality/aging
• premature death
  • mice die at day 20 to 30 of unidentified causes   (MGI Ref ID J:79475)

Dab1^tm1Cpr/Dab1^tm1Cpr

        either: (involves: 129S4/SvJaeSor * BALB/c) or (involves: 129S4/SvJaeSor * C57BL/6)

• nervous system phenotype
• abnormal neuronal migration
  • in vitro migration of neuroblasts and chain formation is disrupted   (MGI Ref ID J:121846)
• abnormal olfactory bulb morphology
  • olfactory bulbs contain only 60% of the number of cells in the granule cell layer compared to in wild-type mice   (MGI Ref ID J:121846)
  • ependymal zone of the bulb contains fewer cells   (MGI Ref ID J:121846)
• • abnormal olfactory bulb granule cell layer morphology
    • fewer cells that contribute to the olfactory bulb are found in the granule cell layer   (MGI Ref ID J:121846)
  • abnormal rostral migratory stream morphology
    • at P17 and P61, rostral migratory streams of (RMS) neuroblasts are severely disrupted and neuroblasts accumulate in the subventricular zone   (MGI Ref ID J:121846)
• abnormal postnatal subventricular zone morphology
  • neuroblasts and glial cells accumulate in the subventricular zone   (MGI Ref ID J:121846)
  • a 15-fold increase in apoptosis in the subventricular zone is observed   (MGI Ref ID J:121846)
• cellular phenotype
• abnormal neuronal migration
  • in vitro migration of neuroblasts and chain formation is disrupted   (MGI Ref ID J:121846)

Dab1^tm1Cpr/Dab1^tm1Cpr

        involves: 129S4/SvJaeSor

• nervous system phenotype
• abnormal cortical marginal zone morphology
  • the neocortical marginal zone is crowded with polymorphic cells unlike in wild-type mice   (MGI Ref ID J:74239)
• abnormal dentate gyrus morphology
  • the dentate gyrus is disrupted   (MGI Ref ID J:74239)
• abnormal hippocampus pyramidal cell layer
  • the pyramidal cells are scattered over a broad region unlike in wild-type mice   (MGI Ref ID J:74239)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Dab1^tm1Cpr related

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Ataxia (Movement) Defects
Cerebellar Defects
Cortical Defects
Neurodevelopmental Defects

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Dab1tm1Cpr
Allele Name		targeted mutation 1, Jonathan A Cooper
Allele Type		Targeted (knock-out)
Common Name(s)		Dab1 ko; Dab1-; mdab1-1;
Mutation Made By		Dr. Jonathan Cooper,   Fred Hutchinson Cancer Research Center
Strain of Origin		129S4/SvJaeSor
ES Cell Line Name		AK7
ES Cell Line Strain		129S4/SvJaeSor
Gene Symbol and Name		Dab1, disabled 1
Chromosome		4
Gene Common Name(s)		AI956902; C630028C02Rik; RIKEN cDNA C630028C02 gene; expressed sequence AI956902; scm; scr; scrambler; yot; yotari;
Molecular Note		A neomycin resistance cassette replaced a genomic fragment containing sequences encoding the first 47 amino acids of the PTB domain. Western blot analysis on brain lysates derived from homozygous mice demonstrated the absence of the protein. [MGI Ref ID J:43647]

Genotyping

Genotyping Information

Genotyping Protocols

Dab1^tm1Cpralternate1, Separated PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Howell BW; Hawkes R; Soriano P; Cooper JA. 1997. Neuronal position in the developing brain is regulated by mouse disabled-1 [see comments] Nature 389(6652):733-7. [PubMed: 9338785]  [MGI Ref ID J:43647]

Additional References

Assadi AH; Zhang G; Beffert U; McNeil RS; Renfro AL; Niu S; Quattrocchi CC; Antalffy BA; Sheldon M; Armstrong DD; Wynshaw-Boris A; Herz J; D'Arcangelo G; Clark GD. 2003. Interaction of reelin signaling and Lis1 in brain development. Nat Genet 35(3):270-6. [PubMed: 14578885]  [MGI Ref ID J:86398]

Brich J; Shie FS; Howell BW; Li R; Tus K; Wakeland EK; Jin LW; Mumby M; Churchill G; Herz J; Cooper JA. 2003. Genetic modulation of tau phosphorylation in the mouse. J Neurosci 23(1):187-92. [PubMed: 12514215]  [MGI Ref ID J:81203]

Hartfuss E; Forster E; Bock HH; Hack MA; Leprince P; Luque JM; Herz J; Frotscher M; Gotz M. 2003. Reelin signaling directly affects radial glia morphology and biochemical maturation. Development 130(19):4597-609. [PubMed: 12925587]  [MGI Ref ID J:84752]

Dab1^tm1Cpr related

Akopians AL; Babayan AH; Beffert U; Herz J; Basbaum AI; Phelps PE. 2008. Contribution of the Reelin signaling pathways to nociceptive processing. Eur J Neurosci 27(3):523-37. [PubMed: 18279306]  [MGI Ref ID J:132269]

Andrade N; Komnenovic V; Blake SM; Jossin Y; Howell B; Goffinet A; Schneider WJ; Nimpf J. 2007. ApoER2/VLDL receptor and Dab1 in the rostral migratory stream function in postnatal neuronal migration independently of Reelin. Proc Natl Acad Sci U S A 104(20):8508-13. [PubMed: 17494763]  [MGI Ref ID J:121846]

Assadi AH; Zhang G; Beffert U; McNeil RS; Renfro AL; Niu S; Quattrocchi CC; Antalffy BA; Sheldon M; Armstrong DD; Wynshaw-Boris A; Herz J; D'Arcangelo G; Clark GD. 2003. Interaction of reelin signaling and Lis1 in brain development. Nat Genet 35(3):270-6. [PubMed: 14578885]  [MGI Ref ID J:86398]

Assadi AH; Zhang G; McNeil R; Clark GD; D'Arcangelo G. 2008. Pafah1b2 mutations suppress the development of hydrocephalus in compound Pafah1b1; Reln and Pafah1b1; Dab1 mutant mice. Neurosci Lett 439(1):100-5. [PubMed: 18514414]  [MGI Ref ID J:137048]

Bock HH; Herz J. 2003. Reelin activates SRC family tyrosine kinases in neurons. Curr Biol 13(1):18-26. [PubMed: 12526740]  [MGI Ref ID J:109819]

Borrell V; Pujadas L; Simo S; Dura D; Sole M; Cooper JA; Del Rio JA; Soriano E. 2007. Reelin and mDab1 regulate the development of hippocampal connections. Mol Cell Neurosci 36(2):158-73. [PubMed: 17720534]  [MGI Ref ID J:126745]

Feng L; Cooper JA. 2009. Dual functions of Dab1 during brain development. Mol Cell Biol 29(2):324-32. [PubMed: 18981215]  [MGI Ref ID J:145031]

Gallagher E; Howell BW; Soriano P; Cooper JA; Hawkes R. 1998. Cerebellar abnormalities in the disabled (mdab1-1) mouse. J Comp Neurol 402(2):238-51. [PubMed: 9845246]  [MGI Ref ID J:51267]

Gavin R; Urena J; Rangel A; Pastrana MA; Requena JR; Soriano E; Aguzzi A; Del Rio JA. 2008. Fibrillar prion peptide PrP(106-126) treatment induces Dab1 phosphorylation and impairs APP processing and A beta production in cortical neurons. Neurobiol Dis 30(2):243-54. [PubMed: 18374587]  [MGI Ref ID J:136832]

Hammond V; So E; Gunnersen J; Valcanis H; Kalloniatis M; Tan SS. 2006. Layer positioning of late-born cortical interneurons is dependent on Reelin but not p35 signaling. J Neurosci 26(5):1646-55. [PubMed: 16452688]  [MGI Ref ID J:105191]

Hartfuss E; Forster E; Bock HH; Hack MA; Leprince P; Luque JM; Herz J; Frotscher M; Gotz M. 2003. Reelin signaling directly affects radial glia morphology and biochemical maturation. Development 130(19):4597-609. [PubMed: 12925587]  [MGI Ref ID J:84752]

Herrick TM; Cooper JA. 2002. A hypomorphic allele of dab1 reveals regional differences in reelin-Dab1 signaling during brain development. Development 129(3):787-96. [PubMed: 11830577]  [MGI Ref ID J:74239]

Herrick TM; Cooper JA. 2004. High affinity binding of Dab1 to Reelin receptors promotes normal positioning of upper layer cortical plate neurons. Brain Res Mol Brain Res 126(2):121-8. [PubMed: 15249135]  [MGI Ref ID J:91679]

Howell BW; Herrick TM; Cooper JA. 1999. Reelin-induced tryosine phosphorylation of disabled 1 during neuronal positioning. Genes Dev 13(6):643-8. [PubMed: 10090720]  [MGI Ref ID J:54100]

Howell BW; Herrick TM; Hildebrand JD; Zhang Y; Cooper JA. 2000. Dab1 tyrosine phosphorylation sites relay positional signals during mouse brain development. Curr Biol 10(15):877-85. [PubMed: 10959835]  [MGI Ref ID J:79475]

Khialeeva E; Lane TF; Carpenter EM. 2011. Disruption of reelin signaling alters mammary gland morphogenesis. Development 138(4):767-76. [PubMed: 21266412]  [MGI Ref ID J:170124]

Maclaurin SA; Krucker T; Fish KN. 2007. Hippocampal dendritic arbor growth in vitro: Regulation by Reelin-Disabled-1 signaling. Brain Res 1172:1-9. [PubMed: 17825270]  [MGI Ref ID J:125733]

Matsuki T; Matthews RT; Cooper JA; van der Brug MP; Cookson MR; Hardy JA; Olson EC; Howell BW. 2010. Reelin and stk25 have opposing roles in neuronal polarization and dendritic Golgi deployment. Cell 143(5):826-36. [PubMed: 21111240]  [MGI Ref ID J:168108]

Niu S; Yabut O; D'Arcangelo G. 2008. The Reelin signaling pathway promotes dendritic spine development in hippocampal neurons. J Neurosci 28(41):10339-48. [PubMed: 18842893]  [MGI Ref ID J:141125]

Palmesino E; Rousso DL; Kao TJ; Klar A; Laufer E; Uemura O; Okamoto H; Novitch BG; Kania A. 2010. Foxp1 and lhx1 coordinate motor neuron migration with axon trajectory choice by gating Reelin signalling. PLoS Biol 8(8):e1000446. [PubMed: 20711475]  [MGI Ref ID J:166792]

Pla R; Borrell V; Flames N; Marin O. 2006. Layer acquisition by cortical GABAergic interneurons is independent of Reelin signaling. J Neurosci 26(26):6924-34. [PubMed: 16807322]  [MGI Ref ID J:110037]

Rice DS; Curran T. 2000. Disabled-1 is expressed in type AII amacrine cells in the mouse retina. J Comp Neurol 424(2):327-38. [PubMed: 10906706]  [MGI Ref ID J:63725]

Tan SS; Kalloniatis M; Truong HT; Binder MD; Cate HS; Kilpatrick TJ; Hammond VE. 2009. Oligodendrocyte positioning in cerebral cortex is independent of projection neuron layering. Glia 57(9):1024-30. [PubMed: 19062175]  [MGI Ref ID J:156212]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	001026 BALB/cByJ
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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