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| This strain lacks MHC class II genes H2-Ab1, H2-Aa, H2-Eb1, H2-Eb2, H2-Ea, serving as an appropriate background for developing xenogenic Class II MHC transgenic strains. | |||||||||||||||||||
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- Disease & phenotype
- Genes & alleles
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Description
Strain Information
Former Names B6.129S-H2dlAb1-Ea (Changed: 20-SEP-07 ) B6.129-H2dlAb1-Ea/J (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Species laboratory mouse Background Strain C57BL/6J Generation N13F2 (18-DEC-12)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Mice that are homozygous null for MHC class II genes H2-Ab1, H2-Aa, H2-Eb1, H2-Eb2, H2-Ea are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. MHC class II gene products (mRNA or protein) are not detected. A dramatic decrease is observed in the number of CD4 positive T cells in thymus, spleen and lymph nodes. This strain should serve as a suitable recipient of xenogenic Class II MHC transgenes allowing the engineering of mouse models of human MHC Class II-associated diseases.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were bred to C57BL/6 mice. The mice were then backcrossed to C57BL/6J for 12 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying H2dlAb1-Ea allele
003374 B6;129S2-H2dlAb1-Ea/J View Strains carrying H2dlAb1-Ea (1 strain)
Phenotype
Phenotype Information
assigned by genotype
H2dlAb1-Ea/H2dlAb1-Ea
B6.129S2-H2dlAb1-Ea/J
- mortality/aging
- increased sensitivity to induced morbidity/mortality
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively (MGI Ref ID J:123934)
- immune system phenotype
- increased susceptibility to bacterial infection
- mice are more susceptible to infection with a monocytotropic Ehrlichia bacteria from Ixodes ovatrus ticks (IOE) than wild-type mice (MGI Ref ID J:123934)
- following infection with either a low or high dose of IOE, mice succumb to the infection at 11 to 15 days and 7 to 9 days, respectively, compared to wild-type mice, which succumb at 14 to 17 days and 8 to 12 days, respectively (MGI Ref ID J:123934)
- mice have higher burdens of Ehrlichia bacteria in all organs following infection than do wild-type mice (MGI Ref ID J:123934)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
H2dlAb1-Ea/H2dlAb1-Ea
involves: 129S2/SvPas * C57BL/6
- immune system phenotype
- *normal* immune system phenotype
- stimulated dendritic cells produce normal amounts of IL-12 and TNF-alpha (MGI Ref ID J:157516)
- hematopoietic system phenotype
- abnormal T cell number (MGI Ref ID J:57484)
Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
MHC class II deficient
Lymphoid Tissue Defects
Internal/Organ Research
Lymphoid Tissue Defects
Research Tools
Immunology and Inflammation Research
MHC class II defects
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | H2dlAb1-Ea | ||
|---|---|---|---|
| Allele Name | targeted deletion, H2 complex | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | IIdelta; MHC II KO; MHC II-; MHC II0; MHC IIdelta; MHC class IIdelta; MHC-IIdelta; MHCII-; MHCIIdelta; | ||
| Mutation Made By | Christophe Benoist, Joslin Diabetes Center | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | H1 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| Molecular Note | A 78.8 kb deletion disrupting Class II MHC genes was induced in 129S2/SvPas-derived H1 embryonic stem (ES) cells via Cre recombination. A hygro-resistance cassette was inserted at the deletion site. The deletion spans from the second exon of the H2-Ab1 gene the third exon of the H2-Ea gene. The H2-Aa, H2-Eb1 and H2-Eb2 genes are completely deleted. [MGI Ref ID J:57484] | ||
Genotyping
Genotyping Information
Genotyping Protocols
H2dlAb1-Ea, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Madsen L; Labrecque N; Engberg J; Dierich A; Svejgaard A; Benoist C; Mathis D; Fugger L. 1999. Mice lacking all conventional MHC class II genes. Proc Natl Acad Sci U S A 96(18):10338-43. [PubMed: 10468609] [MGI Ref ID J:57484]
Additional References
H2dlAb1-Ea relatedAiba Y; Kometani K; Hamadate M; Moriyama S; Sakaue-Sawano A; Tomura M; Luche H; Fehling HJ; Casellas R; Kanagawa O; Miyawaki A; Kurosaki T. 2010. Preferential localization of IgG memory B cells adjacent to contracted germinal centers. Proc Natl Acad Sci U S A 107(27):12192-7. [PubMed: 20547847] [MGI Ref ID J:162089]
Alli R; Nguyen P; Boyd K; Sundberg JP; Geiger TL. 2012. A mouse model of clonal CD8+ T lymphocyte-mediated alopecia areata progressing to alopecia universalis. J Immunol 188(1):477-86. [PubMed: 22116824] [MGI Ref ID J:180590]
Aviszus K; Macleod MK; Kirchenbaum GA; Detanico TO; Heiser RA; St Clair JB; Guo W; Wysocki LJ. 2012. Antigen-specific suppression of humoral immunity by anergic Ars/A1 B cells. J Immunol 189(9):4275-83. [PubMed: 23008448] [MGI Ref ID J:190617]
Barrett NA; Rahman OM; Fernandez JM; Parsons MW; Xing W; Austen KF; Kanaoka Y. 2011. Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes. J Exp Med 208(3):593-604. [PubMed: 21357742] [MGI Ref ID J:176841]
Bienvenu B; Martin B; Auffray C; Cordier C; Becourt C; Lucas B. 2005. Peripheral CD8+CD25+ T lymphocytes from MHC class II-deficient mice exhibit regulatory activity. J Immunol 175(1):246-53. [PubMed: 15972655] [MGI Ref ID J:100582]
Binder CJ; Hartvigsen K; Chang MK; Miller M; Broide D; Palinski W; Curtiss LK; Corr M; Witztum JL. 2004. IL-5 links adaptive and natural immunity specific for epitopes of oxidized LDL and protects from atherosclerosis. J Clin Invest 114(3):427-37. [PubMed: 15286809] [MGI Ref ID J:118092]
Bitsaktsis C; Nandi B; Racine R; MacNamara KC; Winslow G. 2007. T-Cell-independent humoral immunity is sufficient for protection against fatal intracellular ehrlichia infection. Infect Immun 75(10):4933-41. [PubMed: 17664264] [MGI Ref ID J:125283]
Blache C; Adriouch S; Calbo S; Drouot L; Dulauroy S; Arnoult C; Le Corre S; Six A; Seman M; Boyer O. 2009. Cutting edge: CD4-independent development of functional FoxP3+ regulatory t cells. J Immunol 183(7):4182-6. [PubMed: 19767568] [MGI Ref ID J:152754]
Bold TD; Ernst JD. 2012. CD4+ T cell-dependent IFN-gamma production by CD8+ effector T cells in Mycobacterium tuberculosis infection. J Immunol 189(5):2530-6. [PubMed: 22837486] [MGI Ref ID J:189869]
Bosselut R; Feigenbaum L; Sharrow SO; Singer A. 2001. Strength of signaling by CD4 and CD8 coreceptor tails determines the number but not the lineage direction of positively selected thymocytes. Immunity 14(4):483-94. [PubMed: 11336693] [MGI Ref ID J:132432]
Cheng S; Smart M; Hanson J; David CS. 2003. Characterization of HLA DR2 and DQ8 transgenic mouse with a new engineered mouse class II deletion, which lacks all endogenous class II genes. J Autoimmun 21(3):195-9. [PubMed: 14599844] [MGI Ref ID J:86434]
Choi EY; Jung KC; Park HJ; Chung DH; Song JS; Yang SD; Simpson E; Park SH. 2005. Thymocyte-thymocyte interaction for efficient positive selection and maturation of CD4 T cells. Immunity 23(4):387-96. [PubMed: 16226504] [MGI Ref ID J:113276]
Choi YS; Kageyama R; Eto D; Escobar TC; Johnston RJ; Monticelli L; Lao C; Crotty S. 2011. ICOS Receptor Instructs T Follicular Helper Cell versus Effector Cell Differentiation via Induction of the Transcriptional Repressor Bcl6. Immunity 34(6):932-46. [PubMed: 21636296] [MGI Ref ID J:174012]
Conlon TM; Cole JL; Motallebzadeh R; Harper I; Callaghan CJ; Bolton EM; Bradley JA; Saeb-Parsy K; Pettigrew GJ. 2012. Unlinked memory helper responses promote long-lasting humoral alloimmunity. J Immunol 189(12):5703-12. [PubMed: 23162131] [MGI Ref ID J:190849]
Conlon TM; Saeb-Parsy K; Cole JL; Motallebzadeh R; Qureshi MS; Rehakova S; Negus MC; Callaghan CJ; Bolton EM; Bradley JA; Pettigrew GJ. 2012. Germinal center alloantibody responses are mediated exclusively by indirect-pathway CD4 T follicular helper cells. J Immunol 188(6):2643-52. [PubMed: 22323543] [MGI Ref ID J:181855]
Deenick EK; Chan A; Ma CS; Gatto D; Schwartzberg PL; Brink R; Tangye SG. 2010. Follicular helper T cell differentiation requires continuous antigen presentation that is independent of unique B cell signaling. Immunity 33(2):241-53. [PubMed: 20691615] [MGI Ref ID J:163920]
Do JS; Valujskikh A; Vignali DA; Fairchild RL; Min B. 2012. Unexpected role for MHC II-peptide complexes in shaping CD8 T-cell expansion and differentiation in vivo. Proc Natl Acad Sci U S A 109(31):12698-703. [PubMed: 22802622] [MGI Ref ID J:188517]
Do JS; Visperas A; Oh K; Stohlman SA; Min B. 2012. Memory CD4 T cells induce selective expression of IL-27 in CD8+ dendritic cells and regulate homeostatic naive T cell proliferation. J Immunol 188(1):230-7. [PubMed: 22116827] [MGI Ref ID J:180589]
Feuillet V; Lucas B; Di Santo JP; Bismuth G; Trautmann A. 2005. Multiple survival signals are delivered by dendritic cells to naive CD4+ T cells. Eur J Immunol 35(9):2563-72. [PubMed: 16078277] [MGI Ref ID J:113487]
Freeman ML; Burkum CE; Woodland DL; Sun R; Wu TT; Blackman MA. 2012. Importance of antibody in virus infection and vaccine-mediated protection by a latency-deficient recombinant murine gamma-herpesvirus-68. J Immunol 188(3):1049-56. [PubMed: 22198955] [MGI Ref ID J:180759]
Friese MA; Jakobsen KB; Friis L; Etzensperger R; Craner MJ; McMahon RM; Jensen LT; Huygelen V; Jones EY; Bell JI; Fugger L. 2008. Opposing effects of HLA class I molecules in tuning autoreactive CD8+ T cells in multiple sclerosis. Nat Med 14(11):1227-35. [PubMed: 18953350] [MGI Ref ID J:144083]
Getahun A; Smith MJ; Kogut I; van Dyk LF; Cambier JC. 2012. Retention of anergy and inhibition of antibody responses during acute gamma herpesvirus 68 infection. J Immunol 189(6):2965-74. [PubMed: 22904300] [MGI Ref ID J:189918]
Grover HS; Blanchard N; Gonzalez F; Chan S; Robey EA; Shastri N. 2012. The Toxoplasma gondii Peptide AS15 Elicits CD4 T Cells That Can Control Parasite Burden. Infect Immun 80(9):3279-88. [PubMed: 22778097] [MGI Ref ID J:187170]
Guimond M; Veenstra RG; Grindler DJ; Zhang H; Cui Y; Murphy RD; Kim SY; Na R; Hennighausen L; Kurtulus S; Erman B; Matzinger P; Merchant MS; Mackall CL. 2009. Interleukin 7 signaling in dendritic cells regulates the homeostatic proliferation and niche size of CD4+ T cells. Nat Immunol 10(2):149-57. [PubMed: 19136960] [MGI Ref ID J:144504]
Henri S; Poulin LF; Tamoutounour S; Ardouin L; Guilliams M; de Bovis B; Devilard E; Viret C; Azukizawa H; Kissenpfennig A; Malissen B. 2010. CD207+ CD103+ dermal dendritic cells cross-present keratinocyte-derived antigens irrespective of the presence of Langerhans cells. J Exp Med 207(1):189-206, S1-6. [PubMed: 20038600] [MGI Ref ID J:156820]
Houston EG Jr; Fink PJ. 2009. MHC drives TCR repertoire shaping, but not maturation, in recent thymic emigrants. J Immunol 183(11):7244-9. [PubMed: 19915060] [MGI Ref ID J:157387]
Iijima N; Mattei LM; Iwasaki A. 2011. Recruited inflammatory monocytes stimulate antiviral Th1 immunity in infected tissue. Proc Natl Acad Sci U S A 108(1):284-9. [PubMed: 21173243] [MGI Ref ID J:169008]
Ismail N; Crossley EC; Stevenson HL; Walker DH. 2007. Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in ehrlichia-induced immunopathology in murine ehrlichiosis. Infect Immun 75(9):4608-20. [PubMed: 17562770] [MGI Ref ID J:123934]
Jacobsen EA; Zellner KR; Colbert D; Lee NA; Lee JJ. 2011. Eosinophils regulate dendritic cells and Th2 pulmonary immune responses following allergen provocation. J Immunol 187(11):6059-68. [PubMed: 22048766] [MGI Ref ID J:179701]
Kang TW; Yevsa T; Woller N; Hoenicke L; Wuestefeld T; Dauch D; Hohmeyer A; Gereke M; Rudalska R; Potapova A; Iken M; Vucur M; Weiss S; Heikenwalder M; Khan S; Gil J; Bruder D; Manns M; Schirmacher P; Tacke F; Ott M; Luedde T; Longerich T; Kubicka S; Zender L. 2011. Senescence surveillance of pre-malignant hepatocytes limits liver cancer development. Nature 479(7374):547-51. [PubMed: 22080947] [MGI Ref ID J:179823]
Kastenmuller W; Gasteiger G; Subramanian N; Sparwasser T; Busch DH; Belkaid Y; Drexler I; Germain RN. 2011. Regulatory T cells selectively control CD8+ T cell effector pool size via IL-2 restriction. J Immunol 187(6):3186-97. [PubMed: 21849683] [MGI Ref ID J:179230]
Krebs P; Barnes MJ; Lampe K; Whitley K; Bahjat KS; Beutler B; Janssen E; Hoebe K. 2009. NK cell-mediated killing of target cells triggers robust antigen-specific T cell-mediated and humoral responses. Blood 113(26):6593-602. [PubMed: 19406986] [MGI Ref ID J:150150]
Kupfer TM; Crawford ML; Pham K; Gill RG. 2005. MHC-mismatched islet allografts are vulnerable to autoimmune recognition in vivo. J Immunol 175(4):2309-16. [PubMed: 16081800] [MGI Ref ID J:107508]
Larena M; Regner M; Lee E; Lobigs M. 2011. Pivotal role of antibody and subsidiary contribution of CD8+ T cells to recovery from infection in a murine model of Japanese encephalitis. J Virol :. [PubMed: 21450826] [MGI Ref ID J:171204]
Le Campion A; Gagnerault MC; Auffray C; Becourt C; Poitrasson-Riviere M; Lallemand E; Bienvenu B; Martin B; Lepault F; Lucas B. 2009. Lymphopenia-induced spontaneous T-cell proliferation as a cofactor for autoimmune disease development. Blood 114(9):1784-93. [PubMed: 19561321] [MGI Ref ID J:152255]
Le Campion A; Pommier A; Delpoux A; Stouvenel L; Auffray C; Martin B; Lucas B. 2012. IL-2 and IL-7 determine the homeostatic balance between the regulatory and conventional CD4+ T cell compartments during peripheral T cell reconstitution. J Immunol 189(7):3339-46. [PubMed: 22933631] [MGI Ref ID J:190347]
Lee YJ; Jeon YK; Kang BH; Chung DH; Park CG; Shin HY; Jung KC; Park SH. 2010. Generation of PLZF+ CD4+ T cells via MHC class II-dependent thymocyte-thymocyte interaction is a physiological process in humans. J Exp Med 207(1):237-46, S1-7. [PubMed: 20038602] [MGI Ref ID J:156545]
Lemessurier K; Hacker H; Tuomanen E; Redecke V. 2010. Inhibition of T Cells Provides Protection against Early Invasive Pneumococcal Disease. Infect Immun 78(12):5287-94. [PubMed: 20855509] [MGI Ref ID J:165973]
Liu X; Zhan Z; Li D; Xu L; Ma F; Zhang P; Yao H; Cao X. 2011. Intracellular MHC class II molecules promote TLR-triggered innate immune responses by maintaining activation of the kinase Btk. Nat Immunol 12(5):416-24. [PubMed: 21441935] [MGI Ref ID J:171920]
Logunova NN; Viret C; Pobezinsky LA; Miller SA; Kazansky DB; Sundberg JP; Chervonsky AV. 2005. Restricted MHC-peptide repertoire predisposes to autoimmunity. J Exp Med 202(1):73-84. [PubMed: 15998789] [MGI Ref ID J:100625]
London A; Itskovich E; Benhar I; Kalchenko V; Mack M; Jung S; Schwartz M. 2011. Neuroprotection and progenitor cell renewal in the injured adult murine retina requires healing monocyte-derived macrophages. J Exp Med 208(1):23-39. [PubMed: 21220455] [MGI Ref ID J:176855]
Maehr R; Hang HC; Mintern JD; Kim YM; Cuvillier A; Nishimura M; Yamada K; Shirahama-Noda K; Hara-Nishimura I; Ploegh HL. 2005. Asparagine endopeptidase is not essential for class II MHC antigen presentation but is required for processing of cathepsin L in mice. J Immunol 174(11):7066-74. [PubMed: 15905550] [MGI Ref ID J:99005]
Maehr R; Kraus M; Ploegh HL. 2004. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol 34(8):2230-6. [PubMed: 15259020] [MGI Ref ID J:91771]
Majewski M; Bose TO; Sille FC; Pollington AM; Fiebiger E; Boes M. 2007. Protein kinase C delta stimulates antigen presentation by Class II MHC in murine dendritic cells. Int Immunol 19(6):719-32. [PubMed: 17446207] [MGI Ref ID J:122307]
Mangalam A; Rodriguez M; David C. 2006. Role of MHC class II expressing CD4+ T cells in proteolipid protein91-110-induced EAE in HLA-DR3 transgenic mice. Eur J Immunol 36(12):3356-70. [PubMed: 17125142] [MGI Ref ID J:117090]
Martin B; Becourt C; Bienvenu B; Lucas B. 2006. Self-recognition is crucial for maintaining the peripheral CD4+ T-cell pool in a nonlymphopenic environment. Blood 108(1):270-7. [PubMed: 16527889] [MGI Ref ID J:135378]
Martin B; Bourgeois C; Dautigny N; Lucas B. 2003. On the role of MHC class II molecules in the survival and lymphopenia-induced proliferation of peripheral CD4+ T cells. Proc Natl Acad Sci U S A 100(10):6021-6. [PubMed: 12719530] [MGI Ref ID J:126900]
Meyer EH; Goya S; Akbari O; Berry GJ; Savage PB; Kronenberg M; Nakayama T; DeKruyff RH; Umetsu DT. 2006. Glycolipid activation of invariant T cell receptor+ NK T cells is sufficient to induce airway hyperreactivity independent of conventional CD4+ T cells. Proc Natl Acad Sci U S A 103(8):2782-7. [PubMed: 16478801] [MGI Ref ID J:107313]
Mingueneau M; Roncagalli R; Gregoire C; Kissenpfennig A; Miazek A; Archambaud C; Wang Y; Perrin P; Bertosio E; Sansoni A; Richelme S; Locksley RM; Aguado E; Malissen M; Malissen B. 2009. Loss of the LAT adaptor converts antigen-responsive T cells into pathogenic effectors that function independently of the T cell receptor. Immunity 31(2):197-208. [PubMed: 19682930] [MGI Ref ID J:151840]
Mingueneau M; Sansoni A; Gregoire C; Roncagalli R; Aguado E; Weiss A; Malissen M; Malissen B. 2008. The proline-rich sequence of CD3epsilon controls T cell antigen receptor expression on and signaling potency in preselection CD4+CD8+ thymocytes. Nat Immunol 9(5):522-32. [PubMed: 18408722] [MGI Ref ID J:134506]
Montecalvo A; Shufesky WJ; Stolz DB; Sullivan MG; Wang Z; Divito SJ; Papworth GD; Watkins SC; Robbins PD; Larregina AT; Morelli AE. 2008. Exosomes As a Short-Range Mechanism to Spread Alloantigen between Dendritic Cells during T Cell Allorecognition. J Immunol 180(5):3081-90. [PubMed: 18292531] [MGI Ref ID J:131530]
Muller AJ; Filipe-Santos O; Eberl G; Aebischer T; Spath GF; Bousso P. 2012. CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation. Immunity 37(1):147-57. [PubMed: 22727490] [MGI Ref ID J:187413]
Murthy AK; Cong Y; Murphey C; Guentzel MN; Forsthuber TG; Zhong G; Arulanandam BP. 2006. Chlamydial protease-like activity factor induces protective immunity against genital chlamydial infection in transgenic mice that express the human HLA-DR4 allele. Infect Immun 74(12):6722-9. [PubMed: 17015458] [MGI Ref ID J:116949]
Nakamura T; Sonoda KH; Faunce DE; Gumperz J; Yamamura T; Miyake S; Stein-Streilein J. 2003. CD4+ NKT cells, but not conventional CD4+ T cells, are required to generate efferent CD8+ T regulatory cells following antigen inoculation in an immune-privileged site. J Immunol 171(3):1266-71. [PubMed: 12874214] [MGI Ref ID J:120214]
Northrop JK; Thomas RM; Wells AD; Shen H. 2006. Epigenetic remodeling of the IL-2 and IFN-gamma loci in memory CD8 T cells is influenced by CD4 T cells. J Immunol 177(2):1062-9. [PubMed: 16818762] [MGI Ref ID J:134945]
Ohmura-Hoshino M; Matsuki Y; Mito-Yoshida M; Goto E; Aoki-Kawasumi M; Nakayama M; Ohara O; Ishido S. 2009. Cutting edge: requirement of MARCH-I-mediated MHC II ubiquitination for the maintenance of conventional dendritic cells. J Immunol 183(11):6893-7. [PubMed: 19917682] [MGI Ref ID J:157516]
Park JH; Adoro S; Guinter T; Erman B; Alag AS; Catalfamo M; Kimura MY; Cui Y; Lucas PJ; Gress RE; Kubo M; Hennighausen L; Feigenbaum L; Singer A. 2010. Signaling by intrathymic cytokines, not T cell antigen receptors, specifies CD8 lineage choice and promotes the differentiation of cytotoxic-lineage T cells. Nat Immunol 11(3):257-64. [PubMed: 20118929] [MGI Ref ID J:158504]
Poitrasson-Riviere M; Bienvenu B; Le Campion A; Becourt C; Martin B; Lucas B. 2008. Regulatory CD4+ T cells are crucial for preventing CD8+ T cell-mediated autoimmunity. J Immunol 180(11):7294-304. [PubMed: 18490729] [MGI Ref ID J:136309]
Purton JF; Tan JT; Rubinstein MP; Kim DM; Sprent J; Surh CD. 2007. Antiviral CD4+ memory T cells are IL-15 dependent. J Exp Med 204(4):951-61. [PubMed: 17420265] [MGI Ref ID J:125743]
Qian Z; Latham KA; Whittington KB; Miller DC; Brand DD; Rosloniec EF. 2010. An autoantigen-specific, highly restricted T cell repertoire infiltrates the arthritic joints of mice in an HLA-DR1 humanized mouse model of autoimmune arthritis. J Immunol 185(1):110-8. [PubMed: 20511555] [MGI Ref ID J:161436]
Quezada SA; Simpson TR; Peggs KS; Merghoub T; Vider J; Fan X; Blasberg R; Yagita H; Muranski P; Antony PA; Restifo NP; Allison JP. 2010. Tumor-reactive CD4(+) T cells develop cytotoxic activity and eradicate large established melanoma after transfer into lymphopenic hosts. J Exp Med 207(3):637-50. [PubMed: 20156971] [MGI Ref ID J:158560]
Rafei M; Hardy MP; Williams P; Vanegas JR; Forner KA; Dulude G; Labrecque N; Galipeau J; Perreault C. 2011. Development and function of innate polyclonal TCRalphabeta+ CD8+ thymocytes. J Immunol 187(6):3133-44. [PubMed: 21844388] [MGI Ref ID J:179243]
Rajagopalan G; Polich G; Sen MM; Singh M; Epstein BE; Lytle AK; Rouse MS; Patel R; David CS. 2008. Evaluating the role of HLA-DQ polymorphisms on immune response to bacterial superantigens using transgenic mice. Tissue Antigens 71(2):135-45. [PubMed: 18086265] [MGI Ref ID J:148052]
Riddle DS; Miller PJ; Vincent BG; Kepler TB; Maile R; Frelinger JA; Collins EJ. 2008. Rescue of cytotoxic function in the CD8alpha knockout mouse by removal of MHC class II. Eur J Immunol 38(6):1511-21. [PubMed: 18465769] [MGI Ref ID J:136196]
Roman E; Shino H; Qin FX; Liu YJ. 2010. Cutting edge: Hematopoietic-derived APCs select regulatory T cells in thymus. J Immunol 185(7):3819-23. [PubMed: 20802149] [MGI Ref ID J:164214]
Salek-Ardakani S; Arens R; Flynn R; Sette A; Schoenberger SP; Croft M. 2009. Preferential use of B7.2 and not B7.1 in priming of vaccinia virus-specific CD8 T cells. J Immunol 182(5):2909-18. [PubMed: 19234186] [MGI Ref ID J:146248]
Sanapala S; Yu JJ; Murthy AK; Li W; Guentzel MN; Chambers JP; Klose KE; Arulanandam BP. 2012. Perforin- and granzyme-mediated cytotoxic effector functions are essential for protection against Francisella tularensis following vaccination by the defined F. tularensis subsp. novicida DeltafopC vaccine strain. Infect Immun 80(6):2177-85. [PubMed: 22493083] [MGI Ref ID J:186522]
Song A; Song J; Tang X; Croft M. 2007. Cooperation between CD4 and CD8 T cells for anti-tumor activity is enhanced by OX40 signals. Eur J Immunol 37(5):1224-32. [PubMed: 17429847] [MGI Ref ID J:123581]
Stephens GL; Andersson J; Shevach EM. 2007. Distinct subsets of FoxP3+ regulatory T cells participate in the control of immune responses. J Immunol 178(11):6901-11. [PubMed: 17513739] [MGI Ref ID J:147842]
Tamoutounour S; Henri S; Lelouard H; de Bovis B; de Haar C; van der Woude CJ; Woltman AM; Reyal Y; Bonnet D; Sichien D; Bain CC; Mowat AM; Reis E Sousa C; Poulin LF; Malissen B; Guilliams M. 2012. CD64 distinguishes macrophages from dendritic cells in the gut and reveals the Th1-inducing role of mesenteric lymph node macrophages during colitis. Eur J Immunol 42(12):3150-66. [PubMed: 22936024] [MGI Ref ID J:190343]
Taneja V; Behrens M; Basal E; Sparks J; Griffiths MM; Luthra H; David CS. 2008. Delineating the role of the HLA-DR4 'shared epitope' in susceptibility versus resistance to develop arthritis. J Immunol 181(4):2869-77. [PubMed: 18684978] [MGI Ref ID J:140171]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a mixed B6,129S background and is maintained on a congenic B6.129 background. Reproduction is fair. The strain is immunodeficient and sensitive to poor microbiological conditions. Pneumocystis can be a problem. Expected coat color expected from breeding:Black. Mating System Homozygote x Homozygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $145.00 Female or Male Homozygous for H2dlAb1-Ea
Price per Pair (US dollars $) Pair Genotype $290.00 Homozygous for H2dlAb1-Ea x Homozygous for H2dlAb1-Ea Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $188.50 Female or Male Homozygous for H2dlAb1-Ea
Price per Pair (US dollars $) Pair Genotype $377.00 Homozygous for H2dlAb1-Ea x Homozygous for H2dlAb1-Ea Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.