Description

Strain Information

Former Names NOD.B6-Gpi1b/Lt    (Changed: 16-FEB-06 ) NOD.B6-Gpi1b    (Changed: 04-FEB-05 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Background Strain NOD/ShiLt Donor Strain C57BL/6   Donating Investigator Edward Leiter,   The Jackson Laboratory

Appearance
albino
Related Genotype: A/A Tyr^c/Tyr^c Oca2^p/Oca2^p

Description
NOD.B6-(Gpi1-D7Mit346)/LtJ mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Congenic NOD Gpi^b mice become diabetic at a similar rate and onset as NOD/Lt (Chen et al, 2005). Gpi1^b in these mice can be used as a marker for transplant studies.

Development
A C57BL/6 derived, Chr 7 genomic segment extending from Gpi1^b to D7Mit346 was transferred to NOD/Lt (Gpi1^a). An (NODxC57BL/6J)F1 was backcrossed to NOD/Lt for 11 generations with selection for C57BL/6 markers on Chr. 7 prior to inbreeding.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls

Related Strains

Strains carrying   Gpi1^b allele

005730	129S6.CB(B6)-Del(1)1Brk Gpi1b/Gpi1c/BrkMdfJ
000600	B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
000909	B6CBy-Gpi1a/Gpi1b/J or Gpi1a-m1/Gpi1b/J

View Strains carrying   Gpi1^b     (3 strains)

Strains carrying other alleles of Gpi1

002027	129S8/SvEv-Gpi1c Hprt1b-m2/J
000600	B6-Gpi1b x B6CBCa Aw-J/A-T(7;15)9H Gpi1a/J
000377	B6.C-H22c Gpi1a/(HW38)ByJ
000123	B6.C-H24c Gpi1a/(HW54)ByJ
001622	B6.CAST-Gpi1a.Cg-Hbath-J
000797	B6.CAST-Gpi1a/EiJ
006561	B6.CBy(Cg)-Gpi1a-m1J/BrkJ
006562	B6.CBy(Cg)-Gusbmps Gpi1a-m1J/BrkJ
002074	B6.Cg-Gpi1a Hbbd H1b/DehJ
001317	B6.Cg-Igha Thy1a Gpi1a/J
000909	B6CBy-Gpi1a/Gpi1b/J or Gpi1a-m1/Gpi1b/J
002800	C3.Cg-Gpi1a/J
006560	CBy(Cg)-Gpi1a-m1J/BrkJ

View Strains carrying other alleles of Gpi1     (13 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD/ShiLtJ Non-MHC Congenics)

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Research Tools
Diabetes and Obesity Research
Genetics Research (Tissue/Cell Markers)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Gpi1b
Allele Name		b variant
Allele Type		Not Applicable
Gene Symbol and Name		Gpi1, glucose phosphate isomerase 1
Chromosome		7
Gene Common Name(s)		AMF; GNPI; Gpi-1; Gpi-1r; Gpi-1s; Gpi-1t; Gpi1-r; Gpi1-s; Gpi1-t; MF; NK; NK/GPI; NLK; Org; PGI; PHI; SA-36; autocrine motility factor; glucose phosphate isomerase 1, regulatory; glucose phosphate isomerase 1, structural; glucose phosphate isomerase 1, temporal; maturation factor; neuroleukin;
Molecular Note		This allele determines a faster migrating variant of the encoded enzyme and occurs in the wild and in strains BUB/Bn, CBA/J, CBA/CaJ, C3Heb/FeJ, C57BL/6J, C57BL/6J-ob, SEA/GJ and SWR/J. Sequencing analysis identified four nucleotide differences between this allele and Gpi1a. Two differences were silent, while a third difference at position 282 predicts an aspartate for the a allele and an asparagine for the b allele. A fourth difference in the 3' untranslated region was also noted. [MGI Ref ID J:28033]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Chen J; Reifsnyder PC; Scheuplein F; Schott WH; Mileikovsky M; Soodeen-Karamath S; Nagy A; Dosch MH; Ellis J; Koch-Nolte F; Leiter EH. 2005. 'Agouti NOD': identification of a CBA-derived Idd locus on Chromosome 7 and its use for chimera production with NOD embryonic stem cells. Mamm Genome 16(10):775-83. [PubMed: 16261419]  [MGI Ref ID J:102639]

Additional References

Gpi1^b related

Charles DJ; Lee CY. 1980. Biochemical and immunological characterization of genetic variants of phosphoglucose isomerase from mouse. Biochem Genet 18(1-2):153-69. [PubMed: 6155905]  [MGI Ref ID J:6330]

Collinson JM; Quinn JC; Hill RE; West JD. 2003. The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo. Dev Biol 255(2):303-12. [PubMed: 12648492]  [MGI Ref ID J:82632]

DeLorenzo RJ; Ruddle FH. 1969. Genetic control of two electrophoretic variants of glucosephosphate isomerase in the mouse (Mus musculus). Biochem Genet 3(2):151-62. [PubMed: 5364923]  [MGI Ref ID J:5141]

Jay PY; Bielinska M; Erlich JM; Mannisto S; Pu WT; Heikinheimo M; Wilson DB. 2007. Impaired mesenchymal cell function in Gata4 mutant mice leads to diaphragmatic hernias and primary lung defects. Dev Biol 301(2):602-614. [PubMed: 17069789]  [MGI Ref ID J:117367]

Pearce SR; Morgan MJ; Ball S; Peters J; Faik P. 1995. Sequence characterization of alleles Gpi1-Sa and Gpi1-Sb at the glucose phosphate isomerase structural locus. Mamm Genome 6(8):537-9. [PubMed: 8589524]  [MGI Ref ID J:28033]

Szuber N; Buss JL; Soe-Lin S; Felfly H; Trudel M; Ponka P. 2008. Alternative treatment paradigm for thalassemia using iron chelators. Exp Hematol 36(7):773-85. [PubMed: 18456387]  [MGI Ref ID J:136991]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Type 1 Diabetes Repository collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	001976 NOD/ShiLtJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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