Strain Name:

B6.129-Htttm5Mem/J

Stock Number:

003598

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129-Hdtm5Mem/J    (Changed: 13-DEC-07 )
B6.129-Hdhtm4Mem/J    (Changed: 19-SEP-07 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129X1 x 129S1 via R1 (+Kitl-SlJ) ES cell line
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
This strain carries 111 CAG repeat units in the first exon of the endogenous Htt gene. Huntington's-like pathology is seen in the striatum, including nuclear localization of the protein, N-terminal inclusions, and insoluble aggregate formation. Instability of the length of the CAG repeat between generations is seen. Expression of the phenotype occurs earlier than in Htttm4, (Stock No. 003455).

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes and 111 CAG repeat units was used to disrupt exon 1. The construct was electroporated into 129 derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric male animals were crossed to CD1 mice, and then backcrossed to C57BL/6J for 6 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Huntington's Disease Models     (29 strains)

Strains carrying   Htttm5Mem allele
003456   STOCK Htttm5Mem/J
View Strains carrying   Htttm5Mem     (1 strain)

View Strains carrying other alleles of Htt     (12 strains)

Additional Web Information

Visit our Huntington's Disease page for a full listing of Huntington's strains and research services.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Huntington Disease; HD
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Htttm5Mem/Htt+

        involves: 129S1/Sv * 129X1/SvJ * CD-1
  • nervous system phenotype
  • abnormal striatum morphology   (MGI Ref ID J:60937)
    • abnormal medium spiny neuron morphology
      • exhibit relocation of the mutant protein to the nucleus in medium sized spiny neurons and much later, the formation of morphologic nuclear inclusions and insoluble aggregate that are hallmarks of Huntington's Disease in humans, although at a slower rate than in homozygotes   (MGI Ref ID J:60937)
  • neuronal intranuclear inclusions   (MGI Ref ID J:60937)

Htttm5Mem/Htttm5Mem

        involves: 129S1/Sv * 129X1/SvJ * CD-1
  • nervous system phenotype
  • abnormal cerebral cortex morphology
    • QUIN and 3-HK levels are increased in the cortex beginning at 15 months of age, as seen in patients with Huntington disease   (MGI Ref ID J:111237)
  • abnormal nervous system physiology
    • between 8 and 12 weeks of age, striatal mitochondria develops resistance to calcium, becoming equally sensitive to calcium as cortical mitochondria, whereas in wild-type, striatal mitochondria is more sensitive to calcium than cortical mitochondria   (MGI Ref ID J:99425)
  • abnormal striatum morphology   (MGI Ref ID J:60937)
    • the levels of both the endogenous excitotoxin quinolinic acid (QUIN) and its bioprecursor, 3-hydroxykynurenine (3-HK) are increased in the striatum beginning at 15 months of age, similarly to that seen in Huntington disease patients   (MGI Ref ID J:111237)
    • abnormal medium spiny neuron morphology
      • exhibit relocation of the mutant protein to the nucleus in medium sized spiny neurons and much later, the formation of morphologic nuclear inclusions (at around 10 months) and insoluble aggregate that are hallmarks of Huntington's Disease in humans   (MGI Ref ID J:60937)
  • neuronal intranuclear inclusions
    • observed at 10 months   (MGI Ref ID J:60937)
  • behavior/neurological phenotype
  • abnormal behavior
    • onset of persistent rubbing and irritability from 12 to 16 months of age   (MGI Ref ID J:99425)
    • hypoactivity
      • onset of hypoactivity from 12 to 16 months of age   (MGI Ref ID J:99425)
    • limb grasping
      • onset of clasping behavior from 12 to 16 months of age   (MGI Ref ID J:99425)
    • tremors
      • onset of trembling from 12 to 16 months of age   (MGI Ref ID J:99425)

Htttm5Mem/Htttm5Mem

        STOCK Htttm5Mem/J
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice do not exhibit hypoactivity or rearing in open field test that differs significantly from controls   (MGI Ref ID J:185262)
    • mice do not exhibit a deterioration in grip strength as compared to controls   (MGI Ref ID J:185262)
    • mice do not exhibit a decrease in climbing activity   (MGI Ref ID J:185262)
    • stride length, splay length and base are similar to controls   (MGI Ref ID J:185262)
    • dark preference in dark/light choice test is similar to controls   (MGI Ref ID J:185262)
    • abnormal motor coordination/ balance
      • homozygous males perform better than wild type controls on rotarod test   (MGI Ref ID J:185262)
      • homozygous females perform slightly worse than controls at the oldest ages   (MGI Ref ID J:185262)
  • growth/size/body phenotype
  • decreased body weight
    • decrease in body weight is decreased by 28 weeks in both genders   (MGI Ref ID J:185262)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice do not exhibit alterations in startle reflex or in prepulse inhibition   (MGI Ref ID J:185262)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Htttm5Mem related

Neurobiology Research
Cortical Defects
Huntington's disease
Neurodegeneration

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Htttm5Mem
Allele Name targeted mutation 5, Marcy E MacDonald
Allele Type Targeted (knock-in)
Common Name(s) HdhQ111;
Mutation Made ByDr. Marcy MacDonald,   Massachusetts General Hospital
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Promoter Htt, huntingtin, mouse, laboratory
Molecular Note This allele carries 109 CAG repeat units, with 111 glutamines, in the first exon of the endogenous gene. It is a derivative of Htttm8 in which the neo cassette has been removed via cre-mediated recombination. [MGI Ref ID J:52440]

Genotyping

Genotyping Information

Genotyping Protocols

Htttm5Mem, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Wheeler VC; Auerbach W; White JK; Srinidhi J; Auerbach A; Ryan A ; Duyao MP ; Vrbanac V ; Weaver M ; Gusella JF ; Joyner AL ; MacDonald ME. 1999. Length-dependent gametic CAG repeat instability in the Huntington's disease knock-in mouse. Hum Mol Genet 8(1):115-22. [PubMed: 9887339]  [MGI Ref ID J:52440]

Additional References

Wheeler VC; White JK; Gutekunst CA; Vrbanac V; Weaver M; Li XJ; Li SH; Yi H; Vonsattel JP; Gusella JF; Hersch S; Auerbach W; Joyner AL; MacDonald ME. 2000. Long glutamine tracts cause nuclear localization of a novel form of huntingtin in medium spiny striatal neurons in HdhQ92 and HdhQ111 knock-in mice. Hum Mol Genet 9(4):503-13. [PubMed: 10699173]  [MGI Ref ID J:60937]

White JK; Auerbach W; Duyao MP; Vonsattel JP; Gusella JF; Joyner AL ; MacDonald ME. 1997. Huntingtin is required for neurogenesis and is not impaired by the Huntington's disease CAG expansion. Nat Genet 17(4):404-10. [PubMed: 9398841]  [MGI Ref ID J:44391]

Htttm5Mem related

Auerbach W; Hurlbert MS; Hilditch-Maguire P; Wadghiri YZ; Wheeler VC; Cohen SI; Joyner AL; MacDonald ME; Turnbull DH. 2001. The HD mutation causes progressive lethal neurological disease in mice expressing reduced levels of huntingtin. Hum Mol Genet 10(22):2515-23. [PubMed: 11709539]  [MGI Ref ID J:72915]

Brito V; Puigdellivol M; Giralt A; del Toro D; Alberch J; Gines S. 2013. Imbalance of p75(NTR)/TrkB protein expression in Huntington's disease: implication for neuroprotective therapies. Cell Death Dis 4:e595. [PubMed: 23598407]  [MGI Ref ID J:198508]

Brustovetsky N; LaFrance R; Purl KJ; Brustovetsky T; Keene CD; Low WC; Dubinsky JM. 2005. Age-dependent changes in the calcium sensitivity of striatal mitochondria in mouse models of Huntington's Disease. J Neurochem 93(6):1361-70. [PubMed: 15935052]  [MGI Ref ID J:99425]

Chaturvedi RK; Hennessey T; Johri A; Tiwari SK; Mishra D; Agarwal S; Kim YS; Beal MF. 2012. Transducer of regulated CREB-binding proteins (TORCs) transcription and function is impaired in Huntington's disease. Hum Mol Genet 21(15):3474-88. [PubMed: 22589249]  [MGI Ref ID J:185361]

Crook ZR; Housman D. 2011. Huntington's disease: can mice lead the way to treatment? Neuron 69(3):423-35. [PubMed: 21315254]  [MGI Ref ID J:174750]

Crook ZR; Housman DE. 2012. Dysregulation of dopamine receptor D2 as a sensitive measure for Huntington disease pathology in model mice. Proc Natl Acad Sci U S A 109(19):7487-92. [PubMed: 22529362]  [MGI Ref ID J:184816]

Fossale E; Seong IS; Coser KR; Shioda T; Kohane IS; Wheeler VC; Gusella JF; Macdonald ME; Lee JM. 2011. Differential effects of the Huntington's disease CAG mutation in striatum and cerebellum are quantitative not qualitative. Hum Mol Genet 20(21):4258-67. [PubMed: 21840924]  [MGI Ref ID J:176675]

Fossale E; Wheeler VC; Vrbanac V; Lebel LA; Teed A; Mysore JS; Gusella JF; MacDonald ME; Persichetti F. 2002. Identification of a presymptomatic molecular phenotype in Hdh CAG knock-in mice. Hum Mol Genet 11(19):2233-41. [PubMed: 12217951]  [MGI Ref ID J:79126]

Gines S; Bosch M; Marco S; Gavalda N; Diaz-Hernandez M; Lucas JJ; Canals JM; Alberch J. 2006. Reduced expression of the TrkB receptor in Huntington's disease mouse models and in human brain. Eur J Neurosci 23(3):649-58. [PubMed: 16487146]  [MGI Ref ID J:107146]

Gines S; Seong IS; Fossale E; Ivanova E; Trettel F; Gusella JF; Wheeler VC; Persichetti F; MacDonald ME. 2003. Specific progressive cAMP reduction implicates energy deficit in presymptomatic Huntington's disease knock-in mice. Hum Mol Genet 12(5):497-508. [PubMed: 12588797]  [MGI Ref ID J:82117]

Giralt A; Puigdellivol M; Carreton O; Paoletti P; Valero J; Parra-Damas A; Saura CA; Alberch J; Gines S. 2012. Long-term memory deficits in Huntington's disease are associated with reduced CBP histone acetylase activity. Hum Mol Genet 21(6):1203-16. [PubMed: 22116937]  [MGI Ref ID J:181152]

Godin JD; Poizat G; Hickey MA; Maschat F; Humbert S. 2010. Mutant huntingtin-impaired degradation of beta-catenin causes neurotoxicity in Huntington's disease. EMBO J 29(14):2433-45. [PubMed: 20531388]  [MGI Ref ID J:162096]

Goula AV; Berquist BR; Wilson DM 3rd; Wheeler VC; Trottier Y; Merienne K. 2009. Stoichiometry of base excision repair proteins correlates with increased somatic CAG instability in striatum over cerebellum in Huntington's disease transgenic mice. PLoS Genet 5(12):e1000749. [PubMed: 19997493]  [MGI Ref ID J:161747]

Grison A; Mantovani F; Comel A; Agostoni E; Gustincich S; Persichetti F; Del Sal G. 2011. Ser46 phosphorylation and prolyl-isomerase Pin1-mediated isomerization of p53 are key events in p53-dependent apoptosis induced by mutant huntingtin. Proc Natl Acad Sci U S A 108(44):17979-84. [PubMed: 22011578]  [MGI Ref ID J:180055]

Guidetti P; Bates GP; Graham RK; Hayden MR; Leavitt BR; MacDonald ME; Slow EJ; Wheeler VC; Woodman B; Schwarcz R. 2006. Elevated brain 3-hydroxykynurenine and quinolinate levels in Huntington disease mice. Neurobiol Dis 23(1):190-7. [PubMed: 16697652]  [MGI Ref ID J:111237]

Jacobsen JC; Gregory GC; Woda JM; Thompson MN; Coser KR; Murthy V; Kohane IS; Gusella JF; Seong IS; Macdonald ME; Shioda T; Lee JM. 2011. HD CAG-correlated gene expression changes support a simple dominant gain of function. Hum Mol Genet 20(14):2846-60. [PubMed: 21536587]  [MGI Ref ID J:173410]

Keryer G; Pineda JR; Liot G; Kim J; Dietrich P; Benstaali C; Smith K; Cordelieres FP; Spassky N; Ferrante RJ; Dragatsis I; Saudou F. 2011. Ciliogenesis is regulated by a huntingtin-HAP1-PCM1 pathway and is altered in Huntington disease. J Clin Invest 121(11):4372-82. [PubMed: 21985783]  [MGI Ref ID J:178446]

Kovalenko M; Dragileva E; St Claire J; Gillis T; Guide JR; New J; Dong H; Kucherlapati R; Kucherlapati MH; Ehrlich ME; Lee JM; Wheeler VC. 2012. Msh2 acts in medium-spiny striatal neurons as an enhancer of CAG instability and mutant huntingtin phenotypes in Huntington's disease knock-in mice. PLoS One 7(9):e44273. [PubMed: 22970194]  [MGI Ref ID J:191886]

Lee JM; Pinto RM; Gillis T; St Claire JC; Wheeler VC. 2011. Quantification of Age-Dependent Somatic CAG Repeat Instability in Hdh CAG Knock-In Mice Reveals Different Expansion Dynamics in Striatum and Liver. PLoS One 6(8):e23647. [PubMed: 21897851]  [MGI Ref ID J:176142]

Liu CR; Chang CR; Chern Y; Wang TH; Hsieh WC; Shen WC; Chang CY; Chu IC; Deng N; Cohen SN; Cheng TH. 2012. Spt4 is selectively required for transcription of extended trinucleotide repeats. Cell 148(4):690-701. [PubMed: 22341442]  [MGI Ref ID J:181549]

Lloret A; Dragileva E; Teed A; Espinola J; Fossale E; Gillis T; Lopez E; Myers RH; MacDonald ME; Wheeler VC. 2006. Genetic background modifies nuclear mutant huntingtin accumulation and HD CAG repeat instability in Huntington's disease knock-in mice. Hum Mol Genet 15(12):2015-24. [PubMed: 16687439]  [MGI Ref ID J:112063]

Lynch G; Kramar EA; Rex CS; Jia Y; Chappas D; Gall CM; Simmons DA. 2007. Brain-derived neurotrophic factor restores synaptic plasticity in a knock-in mouse model of Huntington's disease J Neurosci 27(16):4424-4434. [PubMed: 17442827]  [MGI Ref ID J:119836]

Marco S; Giralt A; Petrovic MM; Pouladi MA; Martinez-Turrillas R; Martinez-Hernandez J; Kaltenbach LS; Torres-Peraza J; Graham RK; Watanabe M; Lujan R; Nakanishi N; Lipton SA; Lo DC; Hayden MR; Alberch J; Wesseling JF; Perez-Otano I. 2013. Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington's disease models. Nat Med 19(8):1030-8. [PubMed: 23852340]  [MGI Ref ID J:200090]

McFarland KN; Huizenga MN; Darnell SB; Sangrey GR; Berezovska O; Cha JH; Outeiro TF; Sadri-Vakili G. 2014. MeCP2: a novel Huntingtin interactor. Hum Mol Genet 23(4):1036-44. [PubMed: 24105466]  [MGI Ref ID J:205992]

Menalled L; El-Khodor BF; Patry M; Suarez-Farinas M; Orenstein SJ; Zahasky B; Leahy C; Wheeler V; Yang XW; MacDonald M; Morton AJ; Bates G; Leeds J; Park L; Howland D; Signer E; Tobin A; Brunner D. 2009. Systematic behavioral evaluation of Huntington's disease transgenic and knock-in mouse models. Neurobiol Dis 35(3):319-36. [PubMed: 19464370]  [MGI Ref ID J:185262]

Mochel F; Durant B; Meng X; O'Callaghan J; Yu H; Brouillet E; Wheeler VC; Humbert S; Schiffmann R; Durr A. 2012. Early alterations of brain cellular energy homeostasis in huntington disease models. J Biol Chem 287(2):1361-70. [PubMed: 22123819]  [MGI Ref ID J:179665]

Molero AE; Gokhan S; Gonzalez S; Feig JL; Alexandre LC; Mehler MF. 2009. Impairment of developmental stem cell-mediated striatal neurogenesis and pluripotency genes in a knock-in model of Huntington's disease. Proc Natl Acad Sci U S A :. [PubMed: 19955426]  [MGI Ref ID J:155518]

Morfini GA; You YM; Pollema SL; Kaminska A; Liu K; Yoshioka K; Bjorkblom B; Coffey ET; Bagnato C; Han D; Huang CF; Banker G; Pigino G; Brady ST. 2009. Pathogenic huntingtin inhibits fast axonal transport by activating JNK3 and phosphorylating kinesin. Nat Neurosci 12(7):864-71. [PubMed: 19525941]  [MGI Ref ID J:152570]

Orvoen S; Pla P; Gardier AM; Saudou F; David DJ. 2012. Huntington's disease knock-in male mice show specific anxiety-like behaviour and altered neuronal maturation. Neurosci Lett 507(2):127-32. [PubMed: 22178857]  [MGI Ref ID J:180131]

Paoletti P; Vila I; Rife M; Lizcano JM; Alberch J; Gines S. 2008. Dopaminergic and glutamatergic signaling crosstalk in Huntington's disease neurodegeneration: the role of p25/cyclin-dependent kinase 5. J Neurosci 28(40):10090-101. [PubMed: 18829967]  [MGI Ref ID J:143463]

Reis SA; Thompson MN; Lee JM; Fossale E; Kim HH; Liao JK; Moskowitz MA; Shaw SY; Dong L; Haggarty SJ; Macdonald ME; Seong IS. 2011. Striatal neurons expressing full-length mutant huntingtin exhibit decreased N-cadherin and altered neuritogenesis. Hum Mol Genet 20(12):2344-55. [PubMed: 21447599]  [MGI Ref ID J:171983]

Ribeiro FM; Devries RA; Hamilton A; Guimaraes IM; Cregan SP; Pires RG; Ferguson SS. 2014. Metabotropic glutamate receptor 5 knockout promotes motor and biochemical alterations in a mouse model of Huntington's disease. Hum Mol Genet 23(8):2030-42. [PubMed: 24282028]  [MGI Ref ID J:207093]

Ribeiro FM; Paquet M; Ferreira LT; Cregan T; Swan P; Cregan SP; Ferguson SS. 2010. Metabotropic glutamate receptor-mediated cell signaling pathways are altered in a mouse model of Huntington's disease. J Neurosci 30(1):316-24. [PubMed: 20053912]  [MGI Ref ID J:164039]

Saavedra A; Giralt A; Arumi H; Alberch J; Perez-Navarro E. 2013. Regulation of hippocampal cGMP levels as a candidate to treat cognitive deficits in Huntington's disease. PLoS One 8(9):e73664. [PubMed: 24040016]  [MGI Ref ID J:207349]

Saavedra A; Giralt A; Rue L; Xifro X; Xu J; Ortega Z; Lucas JJ; Lombroso PJ; Alberch J; Perez-Navarro E. 2011. Striatal-Enriched Protein Tyrosine Phosphatase Expression and Activity in Huntington's Disease: A STEP in the Resistance to Excitotoxicity. J Neurosci 31(22):8150-8162. [PubMed: 21632937]  [MGI Ref ID J:173377]

Snider BJ; Moss JL; Revilla FJ; Lee CS; Wheeler VC; Macdonald ME; Choi DW. 2003. Neocortical neurons cultured from mice with expanded CAG repeats in the huntingtin gene: unaltered vulnerability to excitotoxins and other insults. Neuroscience 120(3):617-25. [PubMed: 12895502]  [MGI Ref ID J:128151]

Vidal RL; Figueroa A; Court FA; Thielen P; Molina C; Wirth C; Caballero B; Kiffin R; Segura-Aguilar J; Cuervo AM; Glimcher LH; Hetz C. 2012. Targeting the UPR transcription factor XBP1 protects against Huntington's disease through the regulation of FoxO1 and autophagy. Hum Mol Genet 21(10):2245-62. [PubMed: 22337954]  [MGI Ref ID J:183791]

Wheeler VC; Gutekunst CA; Vrbanac V; Lebel LA; Schilling G; Hersch S; Friedlander RM; Gusella JF; Vonsattel JP; Borchelt DR; MacDonald ME. 2002. Early phenotypes that presage late-onset neurodegenerative disease allow testing of modifiers in Hdh CAG knock-in mice. Hum Mol Genet 11(6):633-40. [PubMed: 11912178]  [MGI Ref ID J:75831]

Wheeler VC; Lebel LA; Vrbanac V; Teed A; Te Riele H; MacDonald ME. 2003. Mismatch repair gene Msh2 modifies the timing of early disease in Hdh(Q111) striatum. Hum Mol Genet 12(3):273-81. [PubMed: 12554681]  [MGI Ref ID J:81666]

Wheeler VC; White JK; Gutekunst CA; Vrbanac V; Weaver M; Li XJ; Li SH; Yi H; Vonsattel JP; Gusella JF; Hersch S; Auerbach W; Joyner AL; MacDonald ME. 2000. Long glutamine tracts cause nuclear localization of a novel form of huntingtin in medium spiny striatal neurons in HdhQ92 and HdhQ111 knock-in mice. Hum Mol Genet 9(4):503-13. [PubMed: 10699173]  [MGI Ref ID J:60937]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use


General Terms and Conditions


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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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