Strain Name:

B6.129S4-Cd86tm1Shr/J

Stock Number:

003609

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Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S4 via J1 ES cell line
 
Donating Investigator Arlene H Sharpe,   Harvard Medical School

Description
Cd86 (B7-2) null mice are viable and fertile. Unimmunized mice have normal levels of serum immunoglobulin and normal numbers of B and T lymphocytes. Upon intravenous immunization without adjuvant, they fail to form germinal centers or undergo isotype switching and antigen specific IgG1 and IgG2a isotypes are found to be 5% that of wild type levels. When the immunization route is subcutaneous, IgG1 and IgG2a levels are the same as in wild type mice.

Development
A targeting vector containing a neomycin gene under the control of the mouse phosphoglycerol kinase (PGK) promoter was used to disrupt the exon encoding the Ig-V-like domain in J1 embryonic stem (ES) cells. ES cells were injected into C57BL/6 blastocysts.

SNP (single nucleotide polymorphism) analysis performed by The Jackson Laboratory revealed that the Donating Investigator used C57BL/6N (3 of 4 markers segregating) in the development of the strain. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J at least once to establish the colony.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Cd86tm1Shr allele
004762   NOD.129S4-Cd86tm1Shr/JbsJ
View Strains carrying   Cd86tm1Shr     (1 strain)

Strains carrying other alleles of Cd86
003610   B6.129S4-Cd80tm1Shr Cd86tm2Shr/J
View Strains carrying other alleles of Cd86     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Cd86tm1Shr/Cd86tm1Shr

        B6.129S4-Cd86tm1Shr
  • hematopoietic system phenotype
  • decreased T cell proliferation
    • T cells primed to myelin oligodendrocyte glycoprotein (MOG) 355-55 peptide exhibit modestly reduced proliferative responses as compared to than controls   (MGI Ref ID J:57613)
  • immune system phenotype
  • decreased T cell proliferation
    • T cells primed to myelin oligodendrocyte glycoprotein (MOG) 355-55 peptide exhibit modestly reduced proliferative responses as compared to than controls   (MGI Ref ID J:57613)
  • increased interferon-gamma secretion
    • lymph node cells from mice immunized with myelin oligodendrocyte glycoprotein (MOG) 355-55 peptide produce more interferon gamma than wild type cells   (MGI Ref ID J:57613)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cd86tm1Shr/Cd86tm1Shr

        involves: 129S4/SvJae
  • immune system phenotype
  • abnormal humoral immune response
    • when immunized with trinitrophenyl conjugates of keyhole limpet hemocyanin or ovalbumin, mice produce reduced levels of antibodies compared to wild-type   (MGI Ref ID J:39089)
    • decreased IgG level
      • mice produce less than 5% of wild-type levels of IgG1 and IgG2a when immunized with keyhole limpet hemocyanin or ovalbumin conjugates   (MGI Ref ID J:39089)
  • absent spleen germinal center
    • there is an absence of germinal center formation in mutants   (MGI Ref ID J:39089)
  • hematopoietic system phenotype
  • absent spleen germinal center
    • there is an absence of germinal center formation in mutants   (MGI Ref ID J:39089)
  • decreased IgG level
    • mice produce less than 5% of wild-type levels of IgG1 and IgG2a when immunized with keyhole limpet hemocyanin or ovalbumin conjugates   (MGI Ref ID J:39089)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cd86tm1Shr related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency Associated with Other Defects
Intracellular Signaling Molecules
Lymphoid Tissue Defects

Internal/Organ Research
Lymphoid Tissue Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cd86tm1Shr
Allele Name targeted mutation 1, Arlene H Sharpe
Allele Type Targeted (knock-out)
Common Name(s) B7-2-; B7.2 KO;
Mutation Made By Arlene Sharpe,   Harvard Medical School
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Cd86, CD86 antigen
Chromosome 16
Gene Common Name(s) B7-2; B7.2; B70; CD28 antigen ligand; CD28LG2; Cd28l2; LAB72; Ly-58; Ly58; MB7-2; lymphocyte antigen 58;
Molecular Note A neomycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. [MGI Ref ID J:39089]

Genotyping

Genotyping Information

Genotyping Protocols

Cd86tm1Shralternate1, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Borriello F; Sethna MP; Boyd SD; Schweitzer AN; Tivol EA; Jacoby D ; Strom TB ; Simpson EM ; Freeman GJ ; Sharpe AH. 1997. B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation. Immunity 6(3):303-13. [PubMed: 9075931]  [MGI Ref ID J:39089]

Additional References

Pentcheva-Hoang T; Egen JG; Wojnoonski K; Allison JP. 2004. B7-1 and B7-2 selectively recruit CTLA-4 and CD28 to the immunological synapse. Immunity 21(3):401-13. [PubMed: 15357951]  [MGI Ref ID J:93925]

Cd86tm1Shr related

Ait-Oufella H; Salomon BL; Potteaux S; Robertson AK; Gourdy P; Zoll J; Merval R; Esposito B; Cohen JL; Fisson S; Flavell RA; Hansson GK; Klatzmann D; Tedgui A; Mallat Z. 2006. Natural regulatory T cells control the development of atherosclerosis in mice. Nat Med 12(2):178-80. [PubMed: 16462800]  [MGI Ref ID J:105800]

Arens R; Loewendorf A; Redeker A; Sierro S; Boon L; Klenerman P; Benedict CA; Schoenberger SP. 2011. Differential B7-CD28 Costimulatory Requirements for Stable and Inflationary Mouse Cytomegalovirus-Specific Memory CD8 T Cell Populations. J Immunol 186(7):3874-81. [PubMed: 21357256]  [MGI Ref ID J:170700]

Bak SP; Barnkob MS; Bai A; Higham EM; Wittrup KD; Chen J. 2012. Differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells. J Immunol 189(4):1708-16. [PubMed: 22798683]  [MGI Ref ID J:189743]

Baravalle G; Park H; McSweeney M; Ohmura-Hoshino M; Matsuki Y; Ishido S; Shin JS. 2011. Ubiquitination of CD86 is a key mechanism in regulating antigen presentation by dendritic cells. J Immunol 187(6):2966-73. [PubMed: 21849678]  [MGI Ref ID J:179234]

Borowski AB; Boesteanu AC; Mueller YM; Carafides C; Topham DJ; Altman JD; Jennings SR; Katsikis PD. 2007. Memory CD8+ T cells require CD28 costimulation. J Immunol 179(10):6494-503. [PubMed: 17982038]  [MGI Ref ID J:153868]

Bour-Jordan H; Salomon BL; Thompson HL; Szot GL; Bernhard MR; Bluestone JA. 2004. Costimulation controls diabetes by altering the balance of pathogenic and regulatory T cells. J Clin Invest 114(7):979-87. [PubMed: 15467837]  [MGI Ref ID J:93421]

Bour-Jordan H; Thompson HL; Bluestone JA. 2005. Distinct effector mechanisms in the development of autoimmune neuropathy versus diabetes in nonobese diabetic mice. J Immunol 175(9):5649-55. [PubMed: 16237054]  [MGI Ref ID J:119359]

Buhlmann JE; Elkin SK; Sharpe AH. 2003. A Role for the B7-1/B7-2:CD28/CTLA-4 Pathway During Negative Selection. J Immunol 170(11):5421-8. [PubMed: 12759417]  [MGI Ref ID J:83454]

Chang TT; Jabs C; Sobel RA; Kuchroo VK; Sharpe AH. 1999. Studies in B7-deficient mice reveal a critical role for B7 costimulation in both induction and effector phases of experimental autoimmune encephalomyelitis. J Exp Med 190(5):733-40. [PubMed: 10477557]  [MGI Ref ID J:57613]

Chung Y; Nurieva R; Esashi E; Wang YH; Zhou D; Gapin L; Dong C. 2008. A critical role of costimulation during intrathymic development of invariant NK T cells. J Immunol 180(4):2276-83. [PubMed: 18250436]  [MGI Ref ID J:131999]

Dolfi DV; Duttagupta PA; Boesteanu AC; Mueller YM; Oliai CH; Borowski AB; Katsikis PD. 2011. Dendritic cells and CD28 costimulation are required to sustain virus-specific CD8+ T cell responses during the effector phase in vivo. J Immunol 186(8):4599-608. [PubMed: 21389258]  [MGI Ref ID J:172460]

Duan B; Niu H; Xu Z; Sharpe AH; Croker BP; Sobel ES; Morel L. 2008. Intrafollicular location of marginal zone/CD1d(hi) B cells is associated with autoimmune pathology in a mouse model of lupus. Lab Invest 88(9):1008-20. [PubMed: 18607347]  [MGI Ref ID J:138314]

Garcia CA; Martin M; Michalek SM. 2004. Role of B7 costimulatory molecules in mediating systemic and mucosal antibody responses to attenuated Salmonella enterica serovar Typhimurium and its cloned antigen. Infect Immun 72(10):5824-31. [PubMed: 15385483]  [MGI Ref ID J:93124]

Girvin AM; Dal Canto MC; Rhee L; Salomon B; Sharpe A; Bluestone JA; Miller SD. 2000. A critical role for B7/CD28 costimulation in experimental autoimmune encephalomyelitis: a comparative study using costimulatory molecule-deficient mice and monoclonal antibody blockade. J Immunol 164(1):136-43. [PubMed: 10605004]  [MGI Ref ID J:109892]

Greenwald RJ; Oosterwegel MA; van Der Woude D; Kubal A; Mandelbrot DA; Boussiotis VA; Sharpe AH. 2002. CTLA-4 regulates cell cycle progression during a primary immune response. Eur J Immunol 32(2):366-73. [PubMed: 11807776]  [MGI Ref ID J:74753]

Greenwald RJ; Urban JF; Ekkens MJ; Chen S; Nguyen D; Fang H; Finkelman FD; Sharpe AH; Gause WC. 1999. B7-2 is required for the progression but not the initiation of the type 2 immune response to a gastrointestinal nematode parasite. J Immunol 162(7):4133-9. [PubMed: 10201938]  [MGI Ref ID J:119772]

Hagen KA; Moses CT; Drasler EF; Podetz-Pedersen KM; Jameson SC; Khoruts A. 2004. A role for CD28 in lymphopenia-induced proliferation of CD4 T cells. J Immunol 173(6):3909-15. [PubMed: 15356139]  [MGI Ref ID J:92743]

Hosiawa KA; Wang H; DeVries ME; Garcia B; Liu W; Zhou D; Akram A; Jiang J; Sun H; Cameron MJ; Zhong R; Kelvin DJ. 2005. CD80/CD86 costimulation regulates acute vascular rejection. J Immunol 175(9):6197-204. [PubMed: 16237117]  [MGI Ref ID J:119363]

Kasprowicz DJ; Kohm AP; Berton MT; Chruscinski AJ; Sharpe A; Sanders VM. 2000. Stimulation of the B cell receptor, CD86 (B7-2), and the beta 2-adrenergic receptor intrinsically modulates the level of IgG1 and IgE produced per B cell. J Immunol 165(2):680-90. [PubMed: 10878340]  [MGI Ref ID J:120527]

Kastenmuller W; Gasteiger G; Subramanian N; Sparwasser T; Busch DH; Belkaid Y; Drexler I; Germain RN. 2011. Regulatory T cells selectively control CD8+ T cell effector pool size via IL-2 restriction. J Immunol 187(6):3186-97. [PubMed: 21849683]  [MGI Ref ID J:179230]

Kim G; Levin M; Schoenberger SP; Sharpe A; Kronenberg M. 2007. Paradoxical effect of reduced costimulation in T cell-mediated colitis. J Immunol 178(9):5563-70. [PubMed: 17442938]  [MGI Ref ID J:145837]

Kim HJ; Jung CG; Jensen MA; Dukala D; Soliven B. 2008. Targeting of myelin protein zero in a spontaneous autoimmune polyneuropathy. J Immunol 181(12):8753-60. [PubMed: 19050296]  [MGI Ref ID J:142046]

Kin NW; Sanders VM. 2007. CD86 regulates IgG1 production via a CD19-dependent mechanism. J Immunol 179(3):1516-23. [PubMed: 17641017]  [MGI Ref ID J:149951]

Kin NW; Sanders VM. 2006. CD86 stimulation on a B cell activates the phosphatidylinositol 3-kinase/Akt and phospholipase C gamma 2/protein kinase C alpha beta signaling pathways. J Immunol 176(11):6727-35. [PubMed: 16709832]  [MGI Ref ID J:131798]

Liang B; Gee RJ; Kashgarian MJ; Sharpe AH; Mamula MJ. 1999. B7 costimulation in the development of lupus: autoimmunity arises either in the absence of B7.1/B7.2 or in the presence of anti-b7.1/B7.2 blocking antibodies. J Immunol 163(4):2322-9. [PubMed: 10438978]  [MGI Ref ID J:118772]

Liang B; Kashgarian MJ; Sharpe AH; Mamula MJ. 2000. Autoantibody responses and pathology regulated by B7-1 and B7-2 costimulation in MRL/lpr lupus J Immunol 165(6):3436-43. [PubMed: 10975864]  [MGI Ref ID J:64568]

Litzinger MT; Su Y; Lei TC; Soukhareva N; Scott DW. 2005. Mechanisms of gene therapy for tolerance: B7 signaling is required for peptide-IgG gene-transferred tolerance induction. J Immunol 175(2):780-7. [PubMed: 16002674]  [MGI Ref ID J:100709]

Louvet C; Kabre BG; Davini DW; Martinier N; Su MA; DeVoss JJ; Rosenthal WL; Anderson MS; Bour-Jordan H; Bluestone JA. 2009. A novel myelin P0-specific T cell receptor transgenic mouse develops a fulminant autoimmune peripheral neuropathy. J Exp Med 206(3):507-14. [PubMed: 19221395]  [MGI Ref ID J:146797]

Mandelbrot DA; Oosterwegel MA; Shimizu K; Yamada A; Freeman GJ; Mitchell RN; Sayegh MH; Sharpe AH. 2001. B7-dependent T-cell costimulation in mice lacking CD28 and CTLA4. J Clin Invest 107(7):881-7. [PubMed: 11285307]  [MGI Ref ID J:68642]

Mark DA; Donovan CE; De Sanctis GT; He HZ; Cernadas M; Kobzik L; Perkins DL; Sharpe A; Finn PW. 2000. B7-1 (CD80) and B7-2 (CD86) have complementary roles in mediating allergic pulmonary inflammation and airway hyperresponsiveness. Am J Respir Cell Mol Biol 22(3):265-71. [PubMed: 10696062]  [MGI Ref ID J:114244]

Matlack R; Yeh K; Rosini L; Gonzalez D; Taylor J; Silberman D; Pennello A; Riggs J. 2006. Peritoneal macrophages suppress T-cell activation by amino acid catabolism. Immunology 117(3):386-95. [PubMed: 16476058]  [MGI Ref ID J:107076]

McAdam AJ; Farkash EA; Gewurz BE; Sharpe AH. 2000. B7 costimulation is critical for antibody class switching and CD8(+) cytotoxic T-lymphocyte generation in the host response to vesicular stomatitis virus. J Virol 74(1):203-8. [PubMed: 10590107]  [MGI Ref ID J:125985]

Mencacci A; Montagnoli C; Bacci A; Cenci E; Pitzurra L; Spreca A; Kopf M; Sharpe AH; Romani L. 2002. CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis. J Immunol 169(6):3180-90. [PubMed: 12218136]  [MGI Ref ID J:120204]

Mizoguchi E; Mizoguchi A; Preffer FI; Bhan AK. 2000. Regulatory role of mature B cells in a murine model of inflammatory bowel disease. Int Immunol 12(5):597-605. [PubMed: 10784605]  [MGI Ref ID J:110524]

Montagnoli C; Bacci A; Bozza S; Gaziano R; Mosci P; Sharpe AH; Romani L. 2002. B7/CD28-dependent CD4+CD25+ regulatory T cells are essential components of the memory-protective immunity to Candida albicans. J Immunol 169(11):6298-308. [PubMed: 12444136]  [MGI Ref ID J:118777]

Montagnoli C; Fallarino F; Gaziano R; Bozza S; Bellocchio S; Zelante T; Kurup WP; Pitzurra L; Puccetti P; Romani L. 2006. Immunity and tolerance to Aspergillus involve functionally distinct regulatory T cells and tryptophan catabolism. J Immunol 176(3):1712-23. [PubMed: 16424201]  [MGI Ref ID J:126452]

Nolan A; Kobayashi H; Naveed B; Kelly A; Hoshino Y; Hoshino S; Karulf MR; Rom WN; Weiden MD; Gold JA. 2009. Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS One 4(8):e6600. [PubMed: 19672303]  [MGI Ref ID J:152470]

Nurieva R; Thomas S; Nguyen T; Martin-Orozco N; Wang Y; Kaja MK; Yu XZ; Dong C. 2006. T-cell tolerance or function is determined by combinatorial costimulatory signals. EMBO J 25(11):2623-33. [PubMed: 16724117]  [MGI Ref ID J:109521]

Odobasic D; Kitching AR; Tipping PG; Holdsworth SR. 2005. CD80 and CD86 costimulatory molecules regulate crescentic glomerulonephritis by different mechanisms. Kidney Int 68(2):584-94. [PubMed: 16014035]  [MGI Ref ID J:114308]

Ohmura-Hoshino M; Matsuki Y; Mito-Yoshida M; Goto E; Aoki-Kawasumi M; Nakayama M; Ohara O; Ishido S. 2009. Cutting edge: requirement of MARCH-I-mediated MHC II ubiquitination for the maintenance of conventional dendritic cells. J Immunol 183(11):6893-7. [PubMed: 19917682]  [MGI Ref ID J:157516]

Peperzak V; Vikstrom I; Walker J; Glaser SP; Lepage M; Coquery CM; Erickson LD; Fairfax K; Mackay F; Strasser A; Nutt SL; Tarlinton DM. 2013. Mcl-1 is essential for the survival of plasma cells. Nat Immunol 14(3):290-7. [PubMed: 23377201]  [MGI Ref ID J:193618]

Poussin MA; Tuzun E; Goluszko E; Scott BG; Yang H; Franco JU; Christadoss P. 2003. B7-1 costimulatory molecule is critical for the development of experimental autoimmune myasthenia gravis. J Immunol 170(8):4389-96. [PubMed: 12682276]  [MGI Ref ID J:125435]

Rau FC; Dieter J; Luo Z; Priest SO; Baumgarth N. 2009. B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol 183(12):7661-71. [PubMed: 19933871]  [MGI Ref ID J:157479]

Rozanski CH; Arens R; Carlson LM; Nair J; Boise LH; Chanan-Khan AA; Schoenberger SP; Lee KP. 2011. Sustained antibody responses depend on CD28 function in bone marrow-resident plasma cells. J Exp Med 208(7):1435-46. [PubMed: 21690252]  [MGI Ref ID J:176810]

Ruffner MA; Kim SH; Bianco NR; Francisco LM; Sharpe AH; Robbins PD. 2009. B7-1/2, but not PD-L1/2 molecules, are required on IL-10-treated tolerogenic DC and DC-derived exosomes for in vivo function. Eur J Immunol 39(11):3084-3090. [PubMed: 19757438]  [MGI Ref ID J:154179]

Salek-Ardakani S; Arens R; Flynn R; Sette A; Schoenberger SP; Croft M. 2009. Preferential use of B7.2 and not B7.1 in priming of vaccinia virus-specific CD8 T cells. J Immunol 182(5):2909-18. [PubMed: 19234186]  [MGI Ref ID J:146248]

Salek-Ardakani S; Choi YS; Rafii-El-Idrissi Benhnia M; Flynn R; Arens R; Shoenberger S; Crotty S; Croft M; Salek-Ardakani S. 2011. B cell-specific expression of B7-2 is required for follicular Th cell function in response to vaccinia virus. J Immunol 186(9):5294-303. [PubMed: 21441451]  [MGI Ref ID J:172856]

Salomon B; Rhee L; Bour-Jordan H; Hsin H; Montag A; Soliven B; Arcella J; Girvin AM; Miller SD; Bluestone JA. 2001. Development of spontaneous autoimmune peripheral polyneuropathy in B7-2-deficient NOD mice. J Exp Med 194(5):677-84. [PubMed: 11535635]  [MGI Ref ID J:71352]

Santra S; Barouch DH; Jackson SS; Kuroda MJ; Schmitz JE; Lifton MA; Sharpe AH; Letvin NL. 2000. Functional equivalency of B7-1 and B7-2 for costimulating plasmid DNA vaccine-elicited CTL responses J Immunol 165(12):6791-5. [PubMed: 11120800]  [MGI Ref ID J:66171]

Segura E; Nicco C; Lombard B; Veron P; Raposo G; Batteux F; Amigorena S; Thery C. 2005. ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming. Blood 106(1):216-23. [PubMed: 15790784]  [MGI Ref ID J:107454]

Shimazu T; Iida R; Zhang Q; Welner RS; Medina KL; Alberola-Lla J; Kincade PW. 2012. CD86 is expressed on murine hematopoietic stem cells and denotes lymphopoietic potential. Blood 119(21):4889-97. [PubMed: 22371880]  [MGI Ref ID J:185002]

Shin T; Kennedy G; Gorski K; Tsuchiya H; Koseki H; Azuma M; Yagita H; Chen L; Powell J; Pardoll D; Housseau F. 2003. Cooperative B7-1/2 (CD80/CD86) and B7-DC Costimulation of CD4+ T Cells Independent of the PD-1 Receptor. J Exp Med 198(1):31-8. [PubMed: 12847135]  [MGI Ref ID J:84457]

Sugita S; Keino H; Futagami Y; Takase H; Mochizuki M; Stein-Streilein J; Streilein JW. 2006. B7+ iris pigment epithelial cells convert T cells into CTLA-4+, B7-expressing CD8+ regulatory T cells. Invest Ophthalmol Vis Sci 47(12):5376-84. [PubMed: 17122127]  [MGI Ref ID J:123098]

Sugita S; Ng TF; Schwartzkopff J; Streilein JW. 2004. CTLA-4+CD8+ T cells that encounter B7-2+ iris pigment epithelial cells express their own B7-2 to achieve global suppression of T cell activation. J Immunol 172(7):4184-94. [PubMed: 15034031]  [MGI Ref ID J:88713]

Sugita S; Streilein JW. 2003. Iris pigment epithelium expressing CD86 (B7-2) directly suppresses T cell activation in vitro via binding to cytotoxic T lymphocyte-associated antigen 4. J Exp Med 198(1):161-71. [PubMed: 12835481]  [MGI Ref ID J:84466]

Vacchio MS; Williams JA; Hodes RJ. 2005. A novel role for CD28 in thymic selection: elimination of CD28/B7 interactions increases positive selection. Eur J Immunol 35(2):418-27. [PubMed: 15657954]  [MGI Ref ID J:95546]

Yadav D; Judkowski V; Flodstrom-Tullberg M; Sterling L; Redmond WL; Sherman L; Sarvetnick N. 2004. B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets. J Immunol 173(6):3631-9. [PubMed: 15356107]  [MGI Ref ID J:92756]

Yadav D; Sarvetnick N. 2007. B7-2 regulates survival, phenotype, and function of APCs. J Immunol 178(10):6236-41. [PubMed: 17475851]  [MGI Ref ID J:146121]

Yang J; Riella LV; Chock S; Liu T; Zhao X; Yuan X; Paterson AM; Watanabe T; Vanguri V; Yagita H; Azuma M; Blazar BR; Freeman GJ; Rodig SJ; Sharpe AH; Chandraker A; Sayegh MH. 2011. The novel costimulatory programmed death ligand 1/B7.1 pathway is functional in inhibiting alloimmune responses in vivo. J Immunol 187(3):1113-9. [PubMed: 21697455]  [MGI Ref ID J:179116]

Zeng XL; Nagavalli A; Smith CJ; Howard JF; Su MA. 2013. Divergent effects of T cell costimulation and inflammatory cytokine production on autoimmune peripheral neuropathy provoked by aire deficiency. J Immunol 190(8):3895-904. [PubMed: 23487421]  [MGI Ref ID J:194890]

Zhang P; Martin M; Yang QB; Michalek SM; Katz J. 2004. Role of B7 costimulatory molecules in immune responses and T-helper cell differentiation in response to recombinant HagB from Porphyromonas gingivalis. Infect Immun 72(2):637-44. [PubMed: 14742503]  [MGI Ref ID J:87827]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6/129S4 background. It was backcrossed to B6 for ten generations and has been maintained with intercrosses since N10. It is maintained as a homozygote. Coat color expected from breeding:Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2085.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2710.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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