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Strain Name:

B6.129S4-Cd80tm1Shr/J

Stock Number:

003611

Availability:

Repository- Live

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Genes & Alleles   Cd80;   Cd80tm1Shr;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S4 via J1 ES cell line
Donating Investigator Arlene Sharpe,   Brigham and Women's Hospital
GenerationN10F?+F15N1F3 (04-DEC-07)

Strain Description
Cd80 (B7-1) null mice are viable and fertile. They possess normal numbers of B and T lymphocytes but exhibit a diminished mixed lymphocyte response. Following immunization, antigen specific IgG1, IgG2a and IgM isotypes are 25%-50% that of wild type levels. Survival of certain tissue allografts are slightly prolonged in Cd80 null mice.

Strain Development
A targeting vector containing a neomycin gene under the control of the mouse phosphoglycerol kinase (PGK) promoter was used to disrupt the second exon containing the Ig-V-like domain in J1 embryonic stem (ES) cells. ES cells were injected in to C57BL/6J blastocysts.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Cd80tm1Shr/Cd80tm1Shr

        involves: 129S4/SvJae
  • immune system phenotype
  • abnormal immunoglobulin level (MGI Ref ID J:39089)
    • production of IgG1, IgG2a and IgM is reduced to 25-50% of wild type after immunization with trinitrophenyl conjugates of keyhole limpet hemocyanin or ovalbumin
  • decreased B cell proliferation (MGI Ref ID J:74662)
    • B cells stimulated with LPS and dextran show a mixed lymphocyte response that is reduced by 70% compared to wild type
  • hematopoietic system phenotype
  • decreased B cell proliferation (MGI Ref ID J:74662)
    • B cells stimulated with LPS and dextran show a mixed lymphocyte response that is reduced by 70% compared to wild type

Gene & Allele Details

Allele Symbol Cd80tm1Shr
Allele Name targeted mutation 1, Arlene H Sharpe
Common Name(s) B7-; B7-1-; B7.1; CD80-;
Mutation Made By Arlene Sharpe,   Brigham and Women's Hospital
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Cd80, CD80 antigen
Chromosome 16
Gene Common Name(s) B7-1; B7.1; CD28 antigen ligand; CD28LG; CD28LG1; Cd28l; LAB7; Ly-53; Ly53; lymphocyte antigen 53;
Molecular Note A neomycin selection cassette replaced the exon encoding the IgV-like domain of the protein. RT-PCR analysis on RNA derived from spleen, thymus and LPS-stimulated B cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. FACS analysis on B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. [MGI Ref ID J:74662]

Control Information

  Allele   Control
 Cd80tm1Shr  000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Cd80tm1Shr

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6/129S4 background. It was backcrossed to C57BL/6 for ten generations and has been maintained by intercrosses since N10. It is maintained as a homozygote. Coat color expected from breeding:Black
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   Cd80tm1Shr     (3 strains)

Strains carrying other alleles of Cd80
007769   NOD.FVB-Tg(Igh-6-Cd80)1Gjf/JbsJ
006778   NOD/ShiLt-Tg(GFAP-Cd80)9Mdos/MdosJ
View Strains carrying other alleles of Cd80     (2 strains)

Additional Web Information

Congenic Nomenclature

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cd80tm1Shr related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency Associated with Other Defects
Intracellular Signaling Molecules
Lymphoid Tissue Defects

Internal/Organ Research
Lymphoid Tissue Defects

References

Selected Reference(s)

Freeman GJ; Borriello F; Hodes RJ; Reiser H; Hathcock KS; Laszlo G; McKnight AJ; Kim J; Du L; Lombard DB; et al.. 1993. Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice. Science 262(5135):907-9. [PubMed: 7694362]  [MGI Ref ID J:74662]

Additional References

Price and Supply Information

Strain Name: B6.129S4-Cd80tm1Shr/J
Stock Number: 003611

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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