Strain Name:

B6.129-Grm4tm1Hpn/J

Stock Number:

003619

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129-Gprc1dtm1Hpn    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Metabotropic glutamate receptors are widely distributed throughout the central nervous system. Included in this group is the glutamate receptor, metabotropic 4 (also called mGluR4). The targeted gene product is found at modest levels in several areas of the CNS, but it appears to be expressed at higher levels in the granule cells of the cerebellum. No protein product is detected in the brain by immunological assays. Mutant mice appear normal in size/weight and have normal motor coordination but appear to have an impaired ability to learn complex motor tasks. The donating investigators note that these mice are selectively resistant to chemically-induced absence seizures.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. If necessary, we will modify the strain description as published results become available.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Grm4tm1Hpn allele
003576   STOCK Grm4tm1Hpn/J
View Strains carrying   Grm4tm1Hpn     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Grm4tm1Hpn/Grm4tm1Hpn

        involves: 129S1/Sv * 129X1/SvJ * CD-1
  • behavior/neurological phenotype
  • abnormal motor learning
    • homozygotes improve less with training in the rotating rod motor-learning test compared to wild-type mice; however performance in the open field and bar crossing tests is similar to wild-type   (MGI Ref ID J:35793)
  • nervous system phenotype
  • decreased paired-pulse facilitation
    • paired pulse facilitation is significantly reduced st all interstimulus intervals examined and at greater than 300msec intervals the amplitude of the second EPSC is depressed rather than facilitated as in wild-type mice   (MGI Ref ID J:35793)
    • however, long term depression is similar to wild-type   (MGI Ref ID J:35793)
  • decreased post-tetanic potentiation
    • conditions that stimulate post-tetanic potentiation in wild-type mice induce only post-tetanic depression in most homozygous Purkinje cells   (MGI Ref ID J:35793)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Grm4tm1Hpn related

Neurobiology Research
Behavioral and Learning Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Grm4tm1Hpn
Allele Name targeted mutation 1, David R Hampson
Allele Type Targeted (Null/Knockout)
Common Name(s) Gprc1dtm1Hpn; mGluR4-;
Mutation Made By David Hampson,   University of Toronto
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Grm4, glutamate receptor, metabotropic 4
Chromosome 17
Gene Common Name(s) GPRC1D; MGLUR4; mGlu4;
Molecular Note A neomycin resistance cassette was inserted into an EcoRV site of an exon that encodes a region encompassing Gly 246-Arg 291 of the putative amino terminal extracellular domain of the protein. Immunoblots of cerebella from homozygous mutant mice showed no detectable protein. [MGI Ref ID J:35793]

Genotyping

Genotyping Information

Genotyping Protocols

Grm4tm1Hpn, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Grm4tm1Hpn related

Battaglia G; Busceti CL; Molinaro G; Biagioni F; Traficante A; Nicoletti F; Bruno V. 2006. Pharmacological activation of mGlu4 metabotropic glutamate receptors reduces nigrostriatal degeneration in mice treated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. J Neurosci 26(27):7222-9. [PubMed: 16822979]  [MGI Ref ID J:110229]

Blednov YA; Walker D; Osterndorf-Kahanek E; Harris RA. 2004. Mice lacking metabotropic glutamate receptor 4 do not show the motor stimulatory effect of ethanol. Alcohol 34(2-3):251-9. [PubMed: 15902920]  [MGI Ref ID J:101929]

Bruno V; Battaglia G; Ksiazek I; van der Putten H; Catania MV; Giuffrida R; Lukic S; Leonhardt T; Inderbitzin W; Gasparini F; Kuhn R; Hampson DR; Nicoletti F; Flor PJ. 2000. Selective activation of mGlu4 metabotropic glutamate receptors is protective against excitotoxic neuronal death. J Neurosci 20(17):6413-20. [PubMed: 10964947]  [MGI Ref ID J:64207]

Davis MJ; Haley T; Duvoisin RM; Raber J. 2012. Measures of anxiety, sensorimotor function, and memory in male and female mGluR4(-)/(-) mice. Behav Brain Res 229(1):21-8. [PubMed: 22227508]  [MGI Ref ID J:185091]

Davis MJ; Iancu OD; Acher FC; Stewart BM; Eiwaz MA; Duvoisin RM; Raber J. 2013. Role of mGluR4 in acquisition of fear learning and memory. Neuropharmacology 66:365-72. [PubMed: 22884897]  [MGI Ref ID J:192658]

Fallarino F; Volpi C; Fazio F; Notartomaso S; Vacca C; Busceti C; Bicciato S; Battaglia G; Bruno V; Puccetti P; Fioretti MC; Nicoletti F; Grohmann U; Di Marco R. 2010. Metabotropic glutamate receptor-4 modulates adaptive immunity and restrains neuroinflammation. Nat Med 16(8):897-902. [PubMed: 20657581]  [MGI Ref ID J:163319]

Gerlai R; Roder JC; Hampson DR. 1998. Altered spatial learning and memory in mice lacking the mGluR4 subtype of metabotropic glutamate receptor. Behav Neurosci 112(3):525-32. [PubMed: 9676970]  [MGI Ref ID J:78387]

Iscru E; Goddyn H; Ahmed T; Callaerts-Vegh Z; D'Hooge R; Balschun D. 2013. Improved spatial learning is associated with increased hippocampal but not prefrontal long-term potentiation in mGluR4 knockout mice. Genes Brain Behav 12(6):615-25. [PubMed: 23714430]  [MGI Ref ID J:213401]

Moritz A; Scheschonka A; Beckhaus T; Karas M; Betz H. 2009. Metabotropic glutamate receptor 4 interacts with microtubule-associated protein 1B. Biochem Biophys Res Commun 390(1):82-6. [PubMed: 19781524]  [MGI Ref ID J:155134]

Pekhletski R; Gerlai R; Overstreet LS; Huang XP; Agopyan N; Slater NT; Abramow-Newerly W; Roder JC; Hampson DR. 1996. Impaired cerebellar synaptic plasticity and motor performance in mice lacking the mGluR4 subtype of metabotropic glutamate receptor. J Neurosci 16(20):6364-73. [PubMed: 8815915]  [MGI Ref ID J:35793]

Pitsch J; Schoch S; Gueler N; Flor PJ; van der Putten H; Becker AJ. 2007. Functional role of mGluR1 and mGluR4 in pilocarpine-induced temporal lobe epilepsy. Neurobiol Dis 26(3):623-33. [PubMed: 17446080]  [MGI Ref ID J:141725]

Snead OC 3rd; Banerjee PK; Burnham M; Hampson D. 2000. Modulation of absence seizures by the GABA(A) receptor: a critical rolefor metabotropic glutamate receptor 4 (mGluR4). J Neurosci 20(16):6218-24. [PubMed: 10934271]  [MGI Ref ID J:109464]

Vilar B; Busserolles J; Ling B; Laffray S; Ulmann L; Malhaire F; Chapuy E; Aissouni Y; Etienne M; Bourinet E; Acher F; Pin JP; Eschalier A; Goudet C. 2013. Alleviating pain hypersensitivity through activation of type 4 metabotropic glutamate receptor. J Neurosci 33(48):18951-65. [PubMed: 24285900]  [MGI Ref ID J:204154]

Wang X; Ai J; Hampson DR; Snead OC 3rd. 2005. Altered glutamate and GABA release within thalamocortical circuitry in metabotropic glutamate receptor 4 knockout mice. Neuroscience 134(4):1195-203. [PubMed: 16039800]  [MGI Ref ID J:104424]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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