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Strain Name:

B6C3-Tg(HD82Gln)81Dbo/J

Stock Number:

003627

Availability:

Repository- Live

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General Terms and Conditions
Additional Licensing and Use Restrictions

Former Name      B6C3F1/J-Tg(HD82Gln)81Dbo/J    (Changed: 23-FEB-06 )
Genes & Alleles   HTT;   Prnp;   Tg(HD82Gln)81Dbo;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Mating SystemF1 x Hemizygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator David Borchelt,   McKnight Brain Inst, Univ of Florida
GenerationN25 (31-DEC-07)

Appearance
agouti
Related Genotype: A/?

black
Related Genotype: a/a

Strain Description
Mice expressing this transgene appear normal at birth through 1-2 months. Mice fail to gain weight, develop tremors, hypokinesis and lack coordination. They exhibit an abnormal gait and frequent hind limb clasping. Life expectancy is 5-6 months. Studies using huntingtin antibodies indicated numerous immunoreactive nuclear inclusions in multiple neuron populations. Neuritic damage is evident.

Strain Development
This transgenic line expresses an N-terminally truncated human huntingtin cDNA that encodes 82 glutamines and encompasses the first 171 amino acids. The altered huntingtin cDNA is under control of a mouse prion protein promoter. Expression is observed in neurons of the central nervous system.

Related Disease (OMIM) Terms

Huntington Disease; HD
Mammalian Phenotype Terms assigned by genotype

Tg(HD82Gln)81Dbo/0

        involves: C3H/HeJ * C57BL/6
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:53797)
    • lifespan is approximately 5-6 months
  • behavior/neurological phenotype
  • abnormal gait (MGI Ref ID J:53797)
  • bradykinesia (MGI Ref ID J:53797)
  • impaired coordination (MGI Ref ID J:53797)
  • tremors (MGI Ref ID J:53797)
  • nervous system phenotype
  • abnormal hypothalamus morphology (MGI Ref ID J:84682)
  • neurodegeneration (MGI Ref ID J:84682)
    • degenerated neurons in various hypothalamic areas including the lateral hypothalamus, the ventromedial hypothalamic nucleus, and the and the paraventricular nuclei
  • neuronal intranuclear inclusions (MGI Ref ID J:53797)
    • nuclear inclusions are observed in the cortex and caudate by immunocytochemistry

Gene & Allele Details

Allele Symbol Tg(HD82Gln)81Dbo
Allele Name transgene insertion 81, David R Borchelt
Common Name(s) N171-82Q; TGN(HD82Gln)81Dbo;
Mutation Made By David Borchelt,   McKnight Brain Inst, Univ of Florida
Strain of OriginC3H/B6
Expressed Gene HTT, huntingtin, human
Promoter Prnp, prion protein, mouse, laboratory
Molecular Note This transgenic line expresses an N-terminally truncated human huntingtin cDNA that encodes 82 glutamines and encompasses the first 171 amino acids. The altered huntingtin cDNA is under control of a mouse prion protein promoter, which drives transgene expression in the neurons of the central nervous system [MGI Ref ID J:53797]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(HD82Gln)81Dbo

Colony Maintenance

Breeding & HusbandryThis strain originated on a C3H/B6 background. The strain is maintained by crossing hemizygous males with wildtype B6C3F1/J females. The donating investigator reports that hemizygous females tend to make poor mothers. Expected coat color from breeding:Agouti and Black
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of HTT     (10 strains)

View Strains carrying other alleles of Prnp     (18 strains)

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

HTT related

Developmental Biology Research
Neurodevelopmental Defects

Mouse/Human Gene Homologs
Huntington's disease (chorea)

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cortical Defects
Huntington's disease
Neurodegeneration
Neurodevelopmental Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Tremor Defects

References

Selected Reference(s)

Schilling G; Becher MW; Sharp AH; Jinnah HA; Duan K; Kotzuk JA; Slunt HH; Ratovitski T; Cooper JK; Jenkins NA; Copeland NG; Price DL; Ross CA; Borchelt DR. 1999. Intranuclear inclusions and neuritic aggregates in transgenic mice expressing a mutant N-terminal fragment of huntingtin [published erratum appears in Hum Mol Genet 1999 May;8(5):943] Hum Mol Genet 8(3):397-407. [PubMed: 9949199]  [MGI Ref ID J:53797]

Additional References

Price and Supply Information

Strain Name: B6C3-Tg(HD82Gln)81Dbo/J
Stock Number: 003627

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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