Strain Name:

NOD.129X1-Gad2tm1Bae/J

Stock Number:

003653

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names NOD.129X1-Gad2tm1Bae/J    (Changed: 15-DEC-04 )
NOD/LtJ-Gad2tm1Bae/J    (Changed: 15-DEC-04 )
Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Steinunn Baekkeskov,   UCSF

Description
Gad2-null mice are viable and fertile. Gross appearance is similar to wildtype littermates; brain weight is unaffected, as is cytoarchitecture. Starting as early as 12 weeks of age, homozygous-null mice develop stress-generated seizures induced by cage lid removal and other routine handling. This epileptic syndrome results in increased mortality.
Other phenotypic observations
* defect in the plastcicity of the visual cortex during development
* increased anxiety-like responses in open field and elevated zero maze assays
* diminished response to anxiolytics (anti-anxiety agents)
* Defect in the ability to sustain maximum gamma-aminobutyric acid release during stimulation
Genetic background influences this strain's phenotype. Homozygous-null mice on a mixed NOD,129 background exhibit a higher incidence of apparently less severe seizures than mice on a B6,129 background (also see Stock No. 003654).

Development
Exon 1 is disrupted in this strain. A targeting vector containing the neomycin resistance gene under the control of the phosphoglycerol kinase promoter was introduced into the BamHI site in exon 1 just downstream of the transcription and translation start sites. The construct was electroporated into 129X1/SvJ-derived JM1 embryonic stem cells and selected cells were injected into C57BL/6 blastocysts. No protein product was detected in brain extracts of homozygous-null mice using Western blots.

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Gad2tm1Bae allele
003654   B6.129X1-Gad2tm1Bae/J
View Strains carrying   Gad2tm1Bae     (1 strain)

Strains carrying other alleles of Gad2
023140   B6;129S-Gad2tm1.1Ksvo/J
019022   B6N.Cg-Gad2tm2(cre)Zjh/J
010702   STOCK Gad2tm1(cre/ERT2)Zjh/J
010802   STOCK Gad2tm2(cre)Zjh/J
View Strains carrying other alleles of Gad2     (4 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- No similarity to the expected human disease phenotype was found. One or more human genes are associated with this human disease. The mouse genotype may involve mutations to orthologs of one or more of these genes, but the phenotype did not resemble the disease.
Stiff-Person Syndrome; SPS
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Gad2tm1Bae/Gad2tm1Bae

        involves: 129X1/SvJ * NOD
  • mortality/aging
  • premature death   (MGI Ref ID J:44638)
  • behavior/neurological phenotype
  • seizures
    • exhibit an 11-fold higher incidence of stress-induced seizures than on the C57BL/6 background   (MGI Ref ID J:44638)
    • develop spontaneous seizures   (MGI Ref ID J:44638)
  • nervous system phenotype
  • seizures
    • exhibit an 11-fold higher incidence of stress-induced seizures than on the C57BL/6 background   (MGI Ref ID J:44638)
    • develop spontaneous seizures   (MGI Ref ID J:44638)
  • homeostasis/metabolism phenotype
  • abnormal amino acid level
    • decrease in brain GABA levels unlike the normal levels on the C57BL/6 background, however glucose homeostasis is normal   (MGI Ref ID J:44638)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • normal fertility and development   (MGI Ref ID J:44638)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Gad2tm1Bae related

Neurobiology Research
Epilepsy
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gad2tm1Bae
Allele Name targeted mutation 1, Steinunn Baekkeskov
Allele Type Targeted (Null/Knockout)
Common Name(s) GAD 65 (-); GAD65 -;
Mutation Made By Steinunn Baekkeskov,   UCSF
Strain of Origin129X1/SvJ
ES Cell Line NameJM-1
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Gad2, glutamic acid decarboxylase 2
Chromosome 2
Gene Common Name(s) 6330404F12Rik; GAD(65); GAD65; Gad-2; RIKEN cDNA 6330404F12 gene;
General Note Phenotypic Similarity to Human Syndrome: Epilepsy (J:44638).
Molecular Note A neomycin resistance cassette was inserted into exon 1, downstream of the translation initiation start site. Western blot analysis on brain extracts from homozygous mice confirmed that no detectable protein is expressed from this allele. [MGI Ref ID J:44638]

Genotyping

Genotyping Information

Genotyping Protocols

Gad2tm1Bae, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Gad2tm1Bae related

Blednov YA; Mayfield RD; Belknap J; Harris RA. 2012. Behavioral actions of alcohol: phenotypic relations from multivariate analysis of mutant mouse data. Genes Brain Behav 11(4):424-35. [PubMed: 22405477]  [MGI Ref ID J:198039]

Blednov YA; Walker DL; Iyer SV; Homanics G; Harris AR. 2010. Mice lacking Gad2 show altered behavioral effects of ethanol, flurazepam and gabaxadol. Addict Biol 15(1):45-61. [PubMed: 20002022]  [MGI Ref ID J:185861]

Chattopadhyaya B; Di Cristo G; Wu CZ; Knott G; Kuhlman S; Fu Y; Palmiter RD; Huang ZJ. 2007. GAD67-mediated GABA synthesis and signaling regulate inhibitory synaptic innervation in the visual cortex. Neuron 54(6):889-903. [PubMed: 17582330]  [MGI Ref ID J:126581]

Choi SY; Morales B; Lee HK; Kirkwood A. 2002. Absence of long-term depression in the visual cortex of glutamic Acid decarboxylase-65 knock-out mice. J Neurosci 22(13):5271-6. [PubMed: 12097476]  [MGI Ref ID J:109229]

Fagiolini M; Hensch TK. 2000. Inhibitory threshold for critical-period activation in primary visual cortex. Nature 404(6774):183-6. [PubMed: 10724170]  [MGI Ref ID J:112387]

Hensch TK; Fagiolini M; Mataga N; Stryker MP; Baekkeskov S; Kash SF. 1998. Local GABA circuit control of experience-dependent plasticity in developing visual cortex. Science 282(5393):1504-8. [PubMed: 9822384]  [MGI Ref ID J:124537]

Kanold PO; Kim YA; GrandPre T; Shatz CJ. 2009. Co-regulation of ocular dominance plasticity and NMDA receptor subunit expression in glutamic acid decarboxylase-65 knock-out mice. J Physiol 587(Pt 12):2857-67. [PubMed: 19406876]  [MGI Ref ID J:176796]

Kash SF; Condie BG; Baekkeskov S. 1999. Glutamate decarboxylase and GABA in pancreatic islets: lessons from knock-out mice. Horm Metab Res 31(5):340-4. [PubMed: 10422732]  [MGI Ref ID J:56151]

Kash SF; Johnson RS; Tecott LH; Noebels JL; Mayfield RD; Hanahan D; Baekkeskov S. 1997. Epilepsy in mice deficient in the 65-kDa isoform of glutamic acid decarboxylase. Proc Natl Acad Sci U S A 94(25):14060-5. [PubMed: 9391152]  [MGI Ref ID J:44638]

Kash SF; Tecott LH; Hodge C; Baekkeskov S. 1999. Increased anxiety and altered responses to anxiolytics in mice deficient in the 65-kDa isoform of glutamic acid decarboxylase. Proc Natl Acad Sci U S A 96(4):1698-703. [PubMed: 9990087]  [MGI Ref ID J:53060]

Nahmani M; Turrigiano GG. 2014. Deprivation-induced strengthening of presynaptic and postsynaptic inhibitory transmission in layer 4 of visual cortex during the critical period. J Neurosci 34(7):2571-82. [PubMed: 24523547]  [MGI Ref ID J:206940]

Wu Q; Boyle MP; Palmiter RD. 2009. Loss of GABAergic signaling by AgRP neurons to the parabrachial nucleus leads to starvation. Cell 137(7):1225-34. [PubMed: 19563755]  [MGI Ref ID J:157338]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6,129 background. It is maintained as a heterozygote on a NOD/LtJ background.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001976 NOD/ShiLtJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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