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Strain Name:

STOCK Tg(TIE2GFP)287Sato/J

Stock Number:

003658

Availability:

Level 4

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General Terms and Conditions
Additional Licensing and Use Restrictions

Former Name      FVB/N-TgN(TIE2GFP)287Sato    (Changed: 15-DEC-04 )
      STOCK TgN(TIE2GFP)287Sato/J    (Changed: 15-DEC-04 )
Genes & Alleles   GFP;   Tek;   Tg(TIE2GFP)287Sato;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator Thomas Sato,   Weill Medical College of Cornell Univ
GenerationF?+15 (20-DEC-06)

Strain Description
This strain expresses Green Fluorescent Protein (GFP) under the direction of the endothelial-specific receptor tyrosine kinase (Tek, formerly, Tie2) promoter. Endothelial cells expressing GFP can be visualized via fluorescent microscopy or purified by FACS.

Strain Development
This strain originated on an FVB/N background, however, at some point it may have been outcrossed to the outbred strain CD1. Genetic marker analysis indicates that the strain background is very similar to FVB, however, some non-FVB alleles are present.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(TIE2GFP)287Sato/Tg(TIE2GFP)287Sato

        Background Not Specified
  • normal phenotype
  • no abnormal phenotype detected (MGI Ref ID J:93360)

Gene & Allele Details

Allele Symbol Tg(TIE2GFP)287Sato
Allele Name transgene insertion 287, Thomas N Sato
Common Name(s) TIE2-GFP;
Mutation Made By Thomas Sato,   Weill Medical College of Cornell Univ
Site of ExpressionBlood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS.
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Promoter Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory
General Note Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS.
Molecular Note The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter. [MGI Ref ID J:93360]

Control Information

  Control
   001800 FVB/NJ (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(TIE2GFP)287Sato

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

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003116   STOCK Tg(CAG-EGFP)D4Nagy/J
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View Fluorescent Protein Strains     (139 strains)

Strains carrying   Tg(TIE2GFP)287Sato allele
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
006770   STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
View Strains carrying   Tg(TIE2GFP)287Sato     (2 strains)

Strains carrying other alleles of GFP
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003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
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View Strains carrying other alleles of GFP     (79 strains)

Strains carrying other alleles of Tek
004128   B6.Cg-Tg(Tek-cre)12Flv/J
002856   FVB/N-Tg(TIE2-lacZ)182Sato/J
005707   STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
View Strains carrying other alleles of Tek     (3 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.

Animal Health Reports

Room Number           AX8

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Vascular Defects

Developmental Biology Research
Internal/Organ Defects (vasculature)

Endocrine Deficiency Research
Vasculature Defects

Research Tools
Cancer Research (endothelial cell marker for neovascularization)
Cardiovascular Research (endothelial cell marker for neovascularization)
Developmental Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: endothelial cell markers for neovascularization)
Genetics Research (Tissue/Cell Markers: endothelial cells)
Immunology and Inflammation Research
Neurobiology Research

GFP related

Research Tools
Fluorescent Proteins

References

Selected Reference(s)

Motoike T; Loughna S; Perens E; Roman BL; Liao W; Chau TC; Richardson CD; Kawate T; Kuno J; Weinstein BM; Stainier DY; Sato TN. 2000. Universal GFP reporter for the study of vascular development. Genesis 28(2):75-81. [PubMed: 11064424]  [MGI Ref ID J:93360]

Additional References

Price and Supply Information

Strain Name: STOCK Tg(TIE2GFP)287Sato/J
Stock Number: 003658

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

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Supply Details

Standard SupplyLevel 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one-three months.
Supply Notes Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
Strains that must be genotyped are not available until five to seven weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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