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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names FVB/N-TgN(TIE2GFP)287Sato (Changed: 15-DEC-04 ) STOCK TgN(TIE2GFP)287Sato/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Generation F?+20+F1 (02-JUN-09) Donating Investigator Thomas Sato, Weill Medical College of Cornell Univ
View larger imageDescription
This strain expresses Green Fluorescent Protein (GFP) under the direction of the endothelial-specific receptor tyrosine kinase (Tek, formerly, Tie2) promoter. Endothelial cells expressing GFP can be visualized via fluorescent microscopy or purified by FACS.Development
This strain originated on an FVB/N background, however, at some point it may have been outcrossed to the outbred strain CD1. Genetic marker analysis indicates that the strain background is very similar to FVB, however, some non-FVB alleles are present.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (201 strains)
006770 STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
004128 B6.Cg-Tg(Tek-cre)12Flv/J 008863 B6.Cg-Tg(Tek-cre)1Ywa/J 008537 FVB-Tg(Tek-cre)2352Rwng/J 002856 FVB/N-Tg(TIE2-lacZ)182Sato/J 005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
JAX® NOTES, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(TIE2GFP)287Sato/Tg(TIE2GFP)287Sato
Background Not Specified
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:93360)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TIE2GFP)287Sato | ||
|---|---|---|---|
| Allele Name | transgene insertion 287, Thomas N Sato | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | TIE2-GFP; | ||
| Mutation Made By | Thomas Sato, Weill Medical College of Cornell Univ | ||
| Site of Expression | Blood vessel endothelial cells fluoresce green allowing the visualization of all vessels under fluorescent microscopy or purification of endothelial cells by FACS. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| Gene Symbol and Name | Tg(TIE2GFP)287Sato, transgene insertion 287, Thomas N Sato | ||
| Chromosome | UN | ||
| Gene Common Name(s) | TIE2-GFP; | ||
| General Note | Endothelial cells in transgenic mice express GFP, and can be visualized via fluorescent microscopy or purified by FACS. | ||
| Molecular Note | The transgene contains Green Fluorescent Protein (GFP) under the control of the endothelial specific receptor tyrosine kinase (Tek, previous symbol Tie2) promoter. [MGI Ref ID J:93360] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TIE2GFP)287Sato, MCA, vers. 2
Tg(TIE2GFP)287Sato, QPCR, vers. 2
Tg(TIE2GFP)287Sato, QPCR, vers. 3
Tg(TIE2GFP)287Sato, STD PCR, vers. 1
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Motoike T; Loughna S; Perens E; Roman BL; Liao W; Chau TC; Richardson CD; Kawate T; Kuno J; Weinstein BM; Stainier DY; Sato TN. 2000. Universal GFP reporter for the study of vascular development. Genesis 28(2):75-81. [PubMed: 11064424] [MGI Ref ID J:93360]
Additional References
McKallip RJ; Fisher M; Do Y; Szakal AK; Gunthert U; Nagarkatti PS; Nagarkatti M. 2003. Targeted deletion of CD44v7 exon leads to decreased endothelial cell injury but not tumor cell killing mediated by interleukin-2-activated cytolytic lymphocytes. J Biol Chem 278(44):43818-30. [PubMed: 12904302] [MGI Ref ID J:86375]
Ni H; Denis CV; Subbarao S; Degen JL; Sato TN; Hynes RO; Wagner DD. 2000. Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen. J Clin Invest 106(3):385-92. [PubMed: 10930441] [MGI Ref ID J:63750]
Tg(TIE2GFP)287Sato relatedAddabbo F; Ratliff B; Park HC; Kuo MC; Ungvari Z; Ciszar A; Krasnikof B; Sodhi K; Zhang F; Nasjletti A; Goligorsky MS. 2009. The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach. Am J Pathol 174(1):34-43. [PubMed: 19095954] [MGI Ref ID J:144210]
Ahn GO; Brown JM. 2008. Matrix metalloproteinase-9 is required for tumor vasculogenesis but not for angiogenesis: role of bone marrow-derived myelomonocytic cells. Cancer Cell 13(3):193-205. [PubMed: 18328424] [MGI Ref ID J:132946]
Capoccia BJ; Shepherd RM; Link DC. 2006. G-CSF and AMD3100 mobilize monocytes into the blood that stimulate angiogenesis in vivo through a paracrine mechanism. Blood 108(7):2438-45. [PubMed: 16735597] [MGI Ref ID J:139479]
Ferdous A; Caprioli A; Iacovino M; Martin CM; Morris J; Richardson JA; Latif S; Hammer RE; Harvey RP; Olson EN; Kyba M; Garry DJ. 2009. Nkx2-5 transactivates the Ets-related protein 71 gene and specifies an endothelial/endocardial fate in the developing embryo. Proc Natl Acad Sci U S A 106(3):814-9. [PubMed: 19129488] [MGI Ref ID J:143866]
Fernandez L; Rodriguez S; Huang H; Chora A; Fernandes J; Mumaw C; Cruz E; Pollok K; Cristina F; Price JE; Ferkowicz MJ; Scadden DT; Clauss M; Cardoso AA; Carlesso N. 2008. Tumor necrosis factor-alpha and endothelial cells modulate Notch signaling in the bone marrow microenvironment during inflammation. Exp Hematol 36(5):545-558. [PubMed: 18439488] [MGI Ref ID J:135200]
French WJ; Creemers EE; Tallquist MD. 2008. Platelet-derived growth factor receptors direct vascular development independent of vascular smooth muscle cell function. Mol Cell Biol 28(18):5646-57. [PubMed: 18606782] [MGI Ref ID J:140245]
Gallagher KA; Liu ZJ; Xiao M; Chen H; Goldstein LJ; Buerk DG; Nedeau A; Thom SR; Velazquez OC. 2007. Diabetic impairments in NO-mediated endothelial progenitor cell mobilization and homing are reversed by hyperoxia and SDF-1 alpha. J Clin Invest 117(5):1249-59. [PubMed: 17476357] [MGI Ref ID J:122109]
Horbelt M; Lee SY; Mang HE; Knipe NL; Sado Y; Kribben A; Sutton TA. 2007. Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury. Am J Physiol Renal Physiol 293(3):F688-95. [PubMed: 17626153] [MGI Ref ID J:143820]
Liu L; Cara DC; Kaur J; Raharjo E; Mullaly SC; Jongstra-Bilen J; Jongstra J; Kubes P. 2005. LSP1 is an endothelial gatekeeper of leukocyte transendothelial migration. J Exp Med 201(3):409-18. [PubMed: 15684321] [MGI Ref ID J:96022]
McKallip RJ; Fisher M; Do Y; Szakal AK; Gunthert U; Nagarkatti PS; Nagarkatti M. 2003. Targeted deletion of CD44v7 exon leads to decreased endothelial cell injury but not tumor cell killing mediated by interleukin-2-activated cytolytic lymphocytes. J Biol Chem 278(44):43818-30. [PubMed: 12904302] [MGI Ref ID J:86375]
Ni H; Denis CV; Subbarao S; Degen JL; Sato TN; Hynes RO; Wagner DD. 2000. Persistence of platelet thrombus formation in arterioles of mice lacking both von Willebrand factor and fibrinogen. J Clin Invest 106(3):385-92. [PubMed: 10930441] [MGI Ref ID J:63750]
O'Riordan E; Mendelev N; Patschan S; Patschan D; Eskander J; Cohen-Gould L; Chander P; Goligorsky MS. 2007. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292(1):H285-94. [PubMed: 16963618] [MGI Ref ID J:119956]
Qin G; Ii M; Silver M; Wecker A; Bord E; Ma H; Gavin M; Goukassian DA; Yoon YS; Papayannopoulou T; Asahara T; Kearney M; Thorne T; Curry C; Eaton L; Heyd L; Dinesh D; Kishore R; Zhu Y; Losordo DW. 2006. Functional disruption of alpha4 integrin mobilizes bone marrow-derived endothelial progenitors and augments ischemic neovascularization. J Exp Med 203(1):153-63. [PubMed: 16401693] [MGI Ref ID J:118824]
Sebzda E; Hibbard C; Sweeney S; Abtahian F; Bezman N; Clemens G; Maltzman JS; Cheng L; Liu F; Turner M; Tybulewicz V; Koretzky GA; Kahn ML. 2006. Syk and Slp-76 mutant mice reveal a cell-autonomous hematopoietic cell contribution to vascular development. Dev Cell 11(3):349-61. [PubMed: 16950126] [MGI Ref ID J:112805]
Srinivasan RS; Dillard ME; Lagutin OV; Lin FJ; Tsai S; Tsai MJ; Samokhvalov IM; Oliver G. 2007. Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature. Genes Dev 21(19):2422-2432. [PubMed: 17908929] [MGI Ref ID J:125507]
Uemura A; Kusuhara S; Wiegand SJ; Yu RT; Nishikawa S. 2006. Tlx acts as a proangiogenic switch by regulating extracellular assembly of fibronectin matrices in retinal astrocytes. J Clin Invest 116(2):369-77. [PubMed: 16424942] [MGI Ref ID J:105463]
Wyckoff JB; Wang Y; Lin EY; Li JF; Goswami S; Stanley ER; Segall JE; Pollard JW; Condeelis J. 2007. Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors. Cancer Res 67(6):2649-56. [PubMed: 17363585] [MGI Ref ID J:120318]
Zhang Q; Matsuzaki I; Chatterjee S; Fisher AB. 2005. Activation of endothelial NADPH oxidase during normoxic lung ischemia is KATP channel dependent. Am J Physiol Lung Cell Mol Physiol 289(6):L954-61. [PubMed: 16280460] [MGI Ref ID J:105015]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $66.30 Female or Male Homozygous for Tg(TIE2GFP)287Sato
Pairs /Price (US dollars $) Pair Genotype $132.60 Homozygous for Tg(TIE2GFP)287Sato x Homozygous for Tg(TIE2GFP)287Sato
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $86.20 Female or Male Homozygous for Tg(TIE2GFP)287Sato
Pairs /Price (US dollars $) Pair Genotype $172.40 Homozygous for Tg(TIE2GFP)287Sato x Homozygous for Tg(TIE2GFP)287Sato
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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