Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N?+2   Donating Investigator Leonard Zon,   Children's Hospital

Appearance
white-bellied agouti
Related Genotype: A^w/?

black
Related Genotype: a/a

Description
Mice that are homozygous null for the Map2k4(SEK1, JNKK, or MKK4) gene exhibit liver abnormalities and suffer lethality before embryonic day 14.5. Histological examination reveals a reduced number of hepatocytes and enlarged, hemorrhaging sinuses. The donating investigator speculates that embryo death results from anemia brought on by massive bleeding. SEK1 is an enzyme in the stress-activated MAP kinase pathway.

Control Information

	Control
	101045 B6129SF2/J
Considerations for Choosing Controls

Additional Web Information

JAX^® NOTES, Summer 2005; 498. Toll-like Receptor JAX^® Mice for Immunological Research.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Map2k4^tm1Liz/Map2k4^tm1Liz

        involves: 129S1/Sv

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:48772)
  • death after E12.5
• cardiovascular system phenotype
• internal hemorrhage (MGI Ref ID J:48772)
  • hemorrhaging in the liver region was noted
• liver/biliary system phenotype
• abnormal liver development (MGI Ref ID J:48772)
  • formation of liver bud, but reduction in size; many had two small lobes
• decreased hepatocyte number (MGI Ref ID J:48772)
• enlarged liver sinusoidal spaces (MGI Ref ID J:48772)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Map2k4^tm1Liz related

Cancer Research
Genes Regulating Growth and Proliferation
Growth Factors/Receptors/Cytokines

Developmental Biology Research
Embryonic Lethality (Homozygous)
Internal/Organ Defects (liver)

Hematological Research
Anemia, Iron Deficiency and Transport Defects

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Immunodeficiency
Intracellular Signaling Molecules

Internal/Organ Research
Liver Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Map2k4tm1Liz
Allele Name		targeted mutation 1, Leonard I Zon
Allele Type		Targeted (knock-out)
Common Name(s)		SEK1-/-;
Mutation Made By		Leonard Zon,   Children's Hospital
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		W9.5/W95
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Map2k4, mitogen-activated protein kinase kinase 4
Chromosome		11
Gene Common Name(s)		JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPK/Erk/kinase 1; SEK1; SERK1; Serk1;
Molecular Note		Insertion of a neomycin resistance cassette disrupted two exons encoding part of the catalytic domain of the protein. Western blots of embryonic fibroblasts from homozygous mutant mice showed no detectable protein for the targeted gene. [MGI Ref ID J:48772]

Genotyping

Genotyping Information

Genotyping Protocols

Map2k4 ^tm1Liz, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Map2k4^tm1Liz related

Ganiatsas S; Kwee L; Fujiwara Y; Perkins A; Ikeda T; Labow MA; Zon LI. 1998. SEK1 deficiency reveals mitogen-activated protein kinase cascade crossregulation and leads to abnormal hepatogenesis. Proc Natl Acad Sci U S A 95(12):6881-6. [PubMed: 9618507]  [MGI Ref ID J:48772]

Swat W; Fujikawa K; Ganiatsas S; Yang D; Xavier RJ; Harris NL; Davidson L; Ferrini R; Davis RJ; Labow MA; Flavell RA; Zon LI; Alt FW. 1998. SEK1/MKK4 is required for maintenance of a normal peripheral lymphoid compartment but not for lymphocyte development. Immunity 8(5):625-34. [PubMed: 9620683]  [MGI Ref ID J:77851]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.