Strain Name:

STOCK Tg(MMTV-Cdc37)1Stp/J

Stock Number:

003690

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Mutant Stock; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationN2
 
Donating Investigator Lilia Stepanova,   St. Jude Children's Research Hospital

Description
In wildtype mice Cdc37 is expressed primarily in proliferative tissues. These transgenic mice express Cdc37 under the direction of an MMTV promoter. As a result, levels of transgene mRNA are significantly higher than levels of endogenous Cdc37 in specific tissues (lacrimal, mammary, salivary glands, uterus and testis). Mice are born and develop normally. By 18 months of age females exhibit proliferative disorders including mammary tumors and lymphomas. By 22 months of age 100% of transgenic females develop mammary or lymphoid tumors. Histopathological examination indicates that mammary tumors are adenocarcinomas and adenosquamous carcinomas. Males expressing Cdc37 in mammary tissue develop a system of mammary ducts resembling that of virgin females, but do not develop tumors. Crosses with other MMTV-oncogene expressing mice indicate that Cdc37 can cooperate with myc in transformation of multiple tissues, and with Cyclin D1 in transformation of the mammary epithelium.

Development
A plasmid containing a MMTV promoter, rabbit beta globin splice sequence, mouse Cdc37 open reading frame, and bovine polyadenylation signal was injected into male pronuclei of B6D2F1 mouse embryos. Founder animals were mated with ICR mice.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cdc37
003739   FVB.Cg-Tg(Pbsn-Cdc37)1Stp/J
003689   STOCK Tg(Pbsn-Cdc37)1Stp/J
View Strains carrying other alleles of Cdc37     (2 strains)

View Strains carrying other alleles of MMTV     (18 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Mammary Gland Tumors)
Oncogenes

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(MMTV-Cdc37)1Stp
Allele Name transgene insertion 1, Lilia Stepanova
Allele Type Transgenic (random, expressed)
Common Name(s) MMTV-CDC37.1;
Mutation Made By Lilia Stepanova,   St. Jude Children's Research Hospital
Strain of Origin(C57BL/6 x DBA/2)F1
Expressed Gene Cdc37, cell division cycle 37 homolog (S. cerevisiae), mouse, laboratory
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
General Note Transgenic mice express Cdc37 under the direction of an MMTV promoter. Levels of transgene mRNA are significantly higher than levels of endogenous Cdc37 in specific tissues (lacrimal, mammary, salivary glands, uterus and testis), compared to wild-type mice, which express Cdc37 primarily in proliferative tissues. Mice are born and develop normally. By 18 months of age females exhibit proliferative disorders including mammary tumors and lymphomas. By 22 months of age 100% of transgenic females develop mammary or lymphoid tumors. Histopathological examination indicates that mammary tumors are adenocarcinomas and adenosquamous carcinomas. Males expressing Cdc37 in mammary tissue develop a system of mammary ducts resembling that of virgin females, but do not develop tumors.
Molecular Note The transgene contains the MMTV promoter, rabbit beta globin splice sequence, mouse Cdc37 open reading frame, and bovine polyadenylation signal. [MGI Ref ID J:62465]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Cdc37), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Stepanova L; Finegold M; DeMayo F; Schmidt EV; Harper JW. 2000. The oncoprotein kinase chaperone CDC37 functions as an oncogene in mice and collaborates with both c-myc and cyclin D1 in transformation of multiple tissues Mol Cell Biol 20(12):4462-73. [PubMed: 10825210]  [MGI Ref ID J:62465]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6D2F1 background and was crossed to an ICR strain. It is being described as STOCK. It is maintained as a hemizygote. Expected coat color from breeding:Black,Albino, White Bellied Agouti
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


OncoMouse® requires a license from DuPont, see Licenses for Strains with OncoMouse® Technology.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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