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| These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. This line provides a strong and specific vital marker for axons, and expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other CFP/GFP lines. | |||||||||||||||||||
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 31-DEC-09 Species laboratory mouse Generation N5+N1F14 (04-DEC-07)
Generation DefinitionsDonating Investigator Joshua R Sanes, Harvard University Description
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of Stock No. 003782. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other CFP/GFP lines.Development
A transgenic construct containing a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and backcrossed with wildtype C57BL/6J mice for at least five generations (3/19/00).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
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Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Developmental Biology Research
Fluorescent Proteins
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-YFP)16Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 16, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Thy-1 YFP-16; YFP; YFP-16; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6J x CBA)F1 | ||
| Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Thy1-YFP)16Jrs, transgene insertion 16, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Thy-1 YFP-16; YFP; YFP-16; | ||
| General Note | Transgenic mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. | ||
| Molecular Note | The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. Nine lines were generated (12, 16, 21, A, C, D, F, G, and H). [MGI Ref ID J:93699] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-YFP)HJrs-alternate2,MELT
Tg(Thy1-YFP)16Jrs, Tg(Thy1-YFPH)2Jrs-alternate1,Separated MCA
Tg(Thy1-YFP)16Jrs, Tg(Thy1-YFPH)2Jrs-alternate1, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Misgeld T; Nikic I; Kerschensteiner M. 2007. In vivo imaging of single axons in the mouse spinal cord. Nat Protoc 2(2):263-8. [PubMed: 17406584] [MGI Ref ID J:122714]
Tg(Thy1-YFP)16Jrs relatedAl-Mosawie A; Wilson JM; Brownstone RM. 2007. Heterogeneity of V2-derived interneurons in the adult mouse spinal cord. Eur J Neurosci 26(11):3003-15. [PubMed: 18028108] [MGI Ref ID J:130218]
Bagnall MW; McElvain LE; Faulstich M; du Lac S. 2008. Frequency-independent synaptic transmission supports a linear vestibular behavior. Neuron 60(2):343-52. [PubMed: 18957225] [MGI Ref ID J:149870]
Bagnall MW; Stevens RJ; du Lac S. 2007. Transgenic mouse lines subdivide medial vestibular nucleus neurons into discrete, neurochemically distinct populations. J Neurosci 27(9):2318-30. [PubMed: 17329429] [MGI Ref ID J:118118]
Bishop DL; Misgeld T; Walsh MK; Gan WB; Lichtman JW. 2004. Axon branch removal at developing synapses by axosome shedding. Neuron 44(4):651-61. [PubMed: 15541313] [MGI Ref ID J:130615]
Bogdanik LP; Burgess RW. 2011. A valid mouse model of AGRIN-associated congenital myasthenic syndrome. Hum Mol Genet :. [PubMed: 21890498] [MGI Ref ID J:176117]
Brendza RP; Bacskai BJ; Cirrito JR; Simmons KA; Skoch JM; Klunk WE; Mathis CA; Bales KR; Paul SM; Hyman BT; Holtzman DM. 2005. Anti-Abeta antibody treatment promotes the rapid recovery of amyloid-associated neuritic dystrophy in PDAPP transgenic mice. J Clin Invest 115(2):428-33. [PubMed: 15668737] [MGI Ref ID J:96184]
Burgess RW; Jucius TJ; Ackerman SL. 2006. Motor axon guidance of the mammalian trochlear and phrenic nerves: dependence on the netrin receptor Unc5c and modifier loci. J Neurosci 26(21):5756-66. [PubMed: 16723533] [MGI Ref ID J:109043]
Carrasco DI; Bichler EK; Seburn KL; Pinter MJ. 2010. Nerve terminal degeneration is independent of muscle fiber genotype in SOD1 mice. PLoS One 5(3):e9802. [PubMed: 20339550] [MGI Ref ID J:158879]
Chen PC; Qin LN; Li XM; Walters BJ; Wilson JA; Mei L; Wilson SM. 2009. The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions. J Neurosci 29(35):10909-19. [PubMed: 19726649] [MGI Ref ID J:152454]
Chen Y; Dube CM; Rice CJ; Baram TZ. 2008. Rapid loss of dendritic spines after stress involves derangement of spine dynamics by corticotropin-releasing hormone. J Neurosci 28(11):2903-11. [PubMed: 18337421] [MGI Ref ID J:133020]
Chen YS; Chung SS; Chung SK. 2005. Noninvasive monitoring of diabetes-induced cutaneous nerve fiber loss and hypoalgesia in thy1-YFP transgenic mice. Diabetes 54(11):3112-8. [PubMed: 16249433] [MGI Ref ID J:134031]
Chiu IM; Phatnani H; Kuligowski M; Tapia JC; Carrasco MA; Zhang M; Maniatis T; Carroll MC. 2009. Activation of innate and humoral immunity in the peripheral nervous system of ALS transgenic mice. Proc Natl Acad Sci U S A 106(49):20960-5. [PubMed: 19933335] [MGI Ref ID J:155542]
Comley LH; Wishart TM; Baxter B; Murray LM; Nimmo A; Thomson D; Parson SH; Gillingwater TH. 2011. Induction of cell stress in neurons from transgenic mice expressing yellow fluorescent protein: implications for neurodegeneration research. PLoS One 6(3):e17639. [PubMed: 21408118] [MGI Ref ID J:171713]
David G; Nguyen K; Barrett EF. 2007. Early vulnerability to ischemia/reperfusion injury in motor terminals innervating fast muscles of SOD1-G93A mice. Exp Neurol 204(1):411-20. [PubMed: 17292357] [MGI Ref ID J:119705]
Ey B; Kobsar I; Blazyca H; Kroner A; Martini R. 2007. Visualization of degenerating axons in a dysmyelinating mouse mutant with axonal loss. Mol Cell Neurosci 35(1):153-60. [PubMed: 17383197] [MGI Ref ID J:123228]
Gu C; Rodriguez ER; Reimert DV; Shu T; Fritzsch B; Richards LJ; Kolodkin AL; Ginty DD. 2003. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell 5(1):45-57. [PubMed: 12852851] [MGI Ref ID J:93799]
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Marzban H; Kim CT; Doorn D; Chung SH; Hawkes R. 2008. A novel transverse expression domain in the mouse cerebellum revealed by a neurofilament-associated antigen. Neuroscience 153(4):1190-201. [PubMed: 18455884] [MGI Ref ID J:139387]
McElvain LE; Bagnall MW; Sakatos A; du Lac S. 2010. Bidirectional plasticity gated by hyperpolarization controls the gain of postsynaptic firing responses at central vestibular nerve synapses. Neuron 68(4):763-75. [PubMed: 21092864] [MGI Ref ID J:167744]
Schaefer AM; Sanes JR; Lichtman JW. 2005. A compensatory subpopulation of motor neurons in a mouse model of amyotrophic lateral sclerosis. J Comp Neurol 490(3):209-19. [PubMed: 16082680] [MGI Ref ID J:102773]
Schaefer MK; Schmalbruch H; Buhler E; Lopez C; Martin N; Guenet JL; Haase G. 2007. Progressive motor neuronopathy: a critical role of the tubulin chaperone TBCE in axonal tubulin routing from the Golgi apparatus. J Neurosci 27(33):8779-89. [PubMed: 17699660] [MGI Ref ID J:124178]
Scott CA; Rossiter JP; Andrew RD; Jackson AC. 2008. Structural abnormalities in neurons are sufficient to explain the clinical disease and fatal outcome of experimental rabies in yellow fluorescent protein-expressing transgenic mice. J Virol 82(1):513-21. [PubMed: 17942540] [MGI Ref ID J:130059]
Song JW; Misgeld T; Kang H; Knecht S; Lu J; Cao Y; Cotman SL; Bishop DL; Lichtman JW. 2008. Lysosomal activity associated with developmental axon pruning. J Neurosci 28(36):8993-9001. [PubMed: 18768693] [MGI Ref ID J:141170]
Stella SL Jr; Vila A; Hung AY; Rome ME; Huynh U; Sheng M; Kreienkamp HJ; Brecha NC. 2012. Association of shank 1A scaffolding protein with cone photoreceptor terminals in the mammalian retina. PLoS One 7(9):e43463. [PubMed: 22984429] [MGI Ref ID J:191880]
Unezaki S; Yoshii S; Mabuchi T; Saito A; Ito S. 2009. Effects of neurotrophic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. J Neurosci Methods 178(2):308-15. [PubMed: 19150631] [MGI Ref ID J:151037]
Xu HP; Chen H; Ding Q; Xie ZH; Chen L; Diao L; Wang P; Gan L; Crair MC; Tian N. 2010. The immune protein CD3zeta is required for normal development of neural circuits in the retina. Neuron 65(4):503-15. [PubMed: 20188655] [MGI Ref ID J:167764]
Yano M; Hayakawa-Yano Y; Mele A; Darnell RB. 2010. Nova2 regulates neuronal migration through an RNA switch in disabled-1 signaling. Neuron 66(6):848-58. [PubMed: 20620871] [MGI Ref ID J:163165]
Yu CQ; Rosenblatt MI. 2007. Transgenic corneal neurofluorescence in mice: a new model for in vivo investigation of nerve structure and regeneration. Invest Ophthalmol Vis Sci 48(4):1535-42. [PubMed: 17389482] [MGI Ref ID J:143841]
Zhang Z; Nguyen KT; Barrett EF; David G. 2010. Vesicular ATPase inserted into the plasma membrane of motor terminals by exocytosis alkalinizes cytosolic pH and facilitates endocytosis. Neuron 68(6):1097-108. [PubMed: 21172612] [MGI Ref ID J:167715]
Zhou L; Bar I; Achouri Y; Campbell K; De Backer O; Hebert JM; Jones K; Kessaris N; de Rouvroit CL; O'Leary D; Richardson WD; Goffinet AM; Tissir F. 2008. Early forebrain wiring: genetic dissection using conditional Celsr3 mutant mice. Science 320(5878):946-9. [PubMed: 18487195] [MGI Ref ID J:134879]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, homozygous mice may be bred together. Coat color expected from breeding is Black. Mating System Homozygote x Homozygote (Female x Male) 31-DEC-09 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Tg(Thy1-YFP)16Jrs
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Tg(Thy1-YFP)16Jrs x Homozygous for Tg(Thy1-YFP)16Jrs Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Tg(Thy1-YFP)16Jrs
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Tg(Thy1-YFP)16Jrs x Homozygous for Tg(Thy1-YFP)16Jrs Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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