| |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Hemizygote (Female x Male) 19-SEP-08 Species laboratory mouse Generation N5+N5F14N1F2 (04-DEC-07) Donating Investigator Joshua Sanes, Harvard University Description
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of Stock No. 003782. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other CFP/GFP lines.Development
A transgenic construct containing a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 008299 B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 008636 B6;C-Tg(Prnp-APP695*/EYFP)49Gsn/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 009422 NOD.Cg-Tg(Itgax-EYFP)1Mnz/QtngJ 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Developmental Biology Research
Fluorescent Proteins
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-YFP)16Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 16, Joshua R Sanes | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Thy-1 YFP-16; YFP; YFP-16; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Transgenic mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as in subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. | ||
| Molecular Note | The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The same construct was used to generate Tg(Thy1-YFPH)2Jrs. [MGI Ref ID J:93699] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins -- Generic GFP, QPCR
Tg(Thy1-YFP)16Jrs, Tg(Thy1-YFPH)2Jrs, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Misgeld T; Nikic I; Kerschensteiner M. 2007. In vivo imaging of single axons in the mouse spinal cord. Nat Protoc 2(2):263-8. [PubMed: 17406584] [MGI Ref ID J:122714]
Tg(Thy1-YFP)16Jrs relatedAl-Mosawie A; Wilson JM; Brownstone RM. 2007. Heterogeneity of V2-derived interneurons in the adult mouse spinal cord. Eur J Neurosci 26(11):3003-15. [PubMed: 18028108] [MGI Ref ID J:130218]
Bagnall MW; McElvain LE; Faulstich M; du Lac S. 2008. Frequency-independent synaptic transmission supports a linear vestibular behavior. Neuron 60(2):343-52. [PubMed: 18957225] [MGI Ref ID J:149870]
Bagnall MW; Stevens RJ; du Lac S. 2007. Transgenic mouse lines subdivide medial vestibular nucleus neurons into discrete, neurochemically distinct populations. J Neurosci 27(9):2318-30. [PubMed: 17329429] [MGI Ref ID J:118118]
Bishop DL; Misgeld T; Walsh MK; Gan WB; Lichtman JW. 2004. Axon branch removal at developing synapses by axosome shedding. Neuron 44(4):651-61. [PubMed: 15541313] [MGI Ref ID J:130615]
Brendza RP; Bacskai BJ; Cirrito JR; Simmons KA; Skoch JM; Klunk WE; Mathis CA; Bales KR; Paul SM; Hyman BT; Holtzman DM. 2005. Anti-Abeta antibody treatment promotes the rapid recovery of amyloid-associated neuritic dystrophy in PDAPP transgenic mice. J Clin Invest 115(2):428-33. [PubMed: 15668737] [MGI Ref ID J:96184]
Burgess RW; Jucius TJ; Ackerman SL. 2006. Motor axon guidance of the mammalian trochlear and phrenic nerves: dependence on the netrin receptor Unc5c and modifier loci. J Neurosci 26(21):5756-66. [PubMed: 16723533] [MGI Ref ID J:109043]
Chen PC; Qin LN; Li XM; Walters BJ; Wilson JA; Mei L; Wilson SM. 2009. The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions. J Neurosci 29(35):10909-19. [PubMed: 19726649] [MGI Ref ID J:152454]
Chen Y; Dube CM; Rice CJ; Baram TZ. 2008. Rapid loss of dendritic spines after stress involves derangement of spine dynamics by corticotropin-releasing hormone. J Neurosci 28(11):2903-11. [PubMed: 18337421] [MGI Ref ID J:133020]
Chen YS; Chung SS; Chung SK. 2005. Noninvasive monitoring of diabetes-induced cutaneous nerve fiber loss and hypoalgesia in thy1-YFP transgenic mice. Diabetes 54(11):3112-8. [PubMed: 16249433] [MGI Ref ID J:134031]
David G; Nguyen K; Barrett EF. 2007. Early vulnerability to ischemia/reperfusion injury in motor terminals innervating fast muscles of SOD1-G93A mice. Exp Neurol 204(1):411-20. [PubMed: 17292357] [MGI Ref ID J:119705]
Ey B; Kobsar I; Blazyca H; Kroner A; Martini R. 2007. Visualization of degenerating axons in a dysmyelinating mouse mutant with axonal loss. Mol Cell Neurosci 35(1):153-60. [PubMed: 17383197] [MGI Ref ID J:123228]
Gu C; Rodriguez ER; Reimert DV; Shu T; Fritzsch B; Richards LJ; Kolodkin AL; Ginty DD. 2003. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell 5(1):45-57. [PubMed: 12852851] [MGI Ref ID J:93799]
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Marzban H; Kim CT; Doorn D; Chung SH; Hawkes R. 2008. A novel transverse expression domain in the mouse cerebellum revealed by a neurofilament-associated antigen. Neuroscience 153(4):1190-201. [PubMed: 18455884] [MGI Ref ID J:139387]
Schaefer AM; Sanes JR; Lichtman JW. 2005. A compensatory subpopulation of motor neurons in a mouse model of amyotrophic lateral sclerosis. J Comp Neurol 490(3):209-19. [PubMed: 16082680] [MGI Ref ID J:102773]
Schaefer MK; Schmalbruch H; Buhler E; Lopez C; Martin N; Guenet JL; Haase G. 2007. Progressive motor neuronopathy: a critical role of the tubulin chaperone TBCE in axonal tubulin routing from the Golgi apparatus. J Neurosci 27(33):8779-89. [PubMed: 17699660] [MGI Ref ID J:124178]
Scott CA; Rossiter JP; Andrew RD; Jackson AC. 2008. Structural abnormalities in neurons are sufficient to explain the clinical disease and fatal outcome of experimental rabies in yellow fluorescent protein-expressing transgenic mice. J Virol 82(1):513-21. [PubMed: 17942540] [MGI Ref ID J:130059]
Song JW; Misgeld T; Kang H; Knecht S; Lu J; Cao Y; Cotman SL; Bishop DL; Lichtman JW. 2008. Lysosomal activity associated with developmental axon pruning. J Neurosci 28(36):8993-9001. [PubMed: 18768693] [MGI Ref ID J:141170]
Unezaki S; Yoshii S; Mabuchi T; Saito A; Ito S. 2009. Effects of neurotrophic factors on nerve regeneration monitored by in vivo imaging in thy1-YFP transgenic mice. J Neurosci Methods 178(2):308-15. [PubMed: 19150631] [MGI Ref ID J:151037]
Yu CQ; Rosenblatt MI. 2007. Transgenic corneal neurofluorescence in mice: a new model for in vivo investigation of nerve structure and regeneration. Invest Ophthalmol Vis Sci 48(4):1535-42. [PubMed: 17389482] [MGI Ref ID J:143841]
Zhou L; Bar I; Achouri Y; Campbell K; De Backer O; Hebert JM; Jones K; Kessaris N; de Rouvroit CL; O'Leary D; Richardson WD; Goffinet AM; Tissir F. 2008. Early forebrain wiring: genetic dissection using conditional Celsr3 mutant mice. Science 320(5878):946-9. [PubMed: 18487195] [MGI Ref ID J:134879]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a mixed B6CBAF1 background. It has been backcrossed to C57BL/6J at least five times (3/19/00). Coat color expected from breeding:Black Mating System +/+ sibling x Hemizygote (Female x Male) 19-SEP-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $239.00 Female or Male Hemizygous for Tg(Thy1-YFP)16Jrs
Pairs /Price (US dollars $) Pair Genotype $293.35 Hemizygous for Tg(Thy1-YFP)16Jrs x Noncarrier $293.35 Noncarrier x Hemizygous for Tg(Thy1-YFP)16Jrs $478.00 Hemizygous for Tg(Thy1-YFP)16Jrs x Hemizygous for Tg(Thy1-YFP)16Jrs
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $310.70 Female or Male Hemizygous for Tg(Thy1-YFP)16Jrs
Pairs /Price (US dollars $) Pair Genotype $381.40 Hemizygous for Tg(Thy1-YFP)16Jrs x Noncarrier $381.40 Noncarrier x Hemizygous for Tg(Thy1-YFP)16Jrs $621.40 Hemizygous for Tg(Thy1-YFP)16Jrs x Hemizygous for Tg(Thy1-YFP)16Jrs
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.