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- Description
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- Genes & alleles
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Background Strain C57BL/6J Donor Strain B6CBAF1 Generation N5+N17 (30-DEC-08) Donating Investigator Joshua Sanes, Harvard University Description
These mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the termials. No expression is detectable in nonneural cells. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other YFP/GFP lines.Development
A transgenic construct containing a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 006617 B6;CBA-Tg(Thy1-CFP/COX8A)S2Lich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-ECFP)24Hara/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
CFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-CFP)23Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 23, Joshua R Sanes | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Thy-1-CFP; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Cyan Fluorescent Protein (CFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When CFP is utilized in a transgenic construct, tissue expressing sufficient amounts of CFP will fluoresce cyan when exposed to a 433 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Transgenic mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. | ||
| Molecular Note | The transgene contains a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. [MGI Ref ID J:93699] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-CFP)23Jrs, Standard PCR
Tg(Thy1-CFP)23Jrs, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Tg(Thy1-CFP)23Jrs relatedBernstein SL; Guo Y; Slater BJ; Puche A; Kelman SE. 2007. Neuron stress and loss following rodent anterior ischemic optic neuropathy in double-reporter transgenic mice. Invest Ophthalmol Vis Sci 48(5):2304-10. [PubMed: 17460295] [MGI Ref ID J:123308]
Dratviman-Storobinsky O; Hasanreisoglu M; Offen D; Barhum Y; Weinberger D; Goldenberg-Cohen N. 2008. Progressive damage along the optic nerve following induction of crush injury or rodent anterior ischemic optic neuropathy in transgenic mice. Mol Vis 14:2171-9. [PubMed: 19052651] [MGI Ref ID J:143239]
Gastinger MJ; Kunselman AR; Conboy EE; Bronson SK; Barber AJ. 2008. Dendrite remodeling and other abnormalities in the retinal ganglion cells of Ins2 Akita diabetic mice. Invest Ophthalmol Vis Sci 49(6):2635-42. [PubMed: 18515593] [MGI Ref ID J:137045]
Howell GR; Libby RT; Jakobs TC; Smith RS; Phalan FC; Barter JW; Barbay JM; Marchant JK; Mahesh N; Porciatti V; Whitmore AV; Masland RH; John SW. 2007. Axons of retinal ganglion cells are insulted in the optic nerve early in DBA/2J glaucoma. J Cell Biol 179(7):1523-37. [PubMed: 18158332] [MGI Ref ID J:131073]
Inokuchi Y; Nakajima Y; Shimazawa M; Kurita T; Kubo M; Saito A; Sajiki H; Kudo T; Aihara M; Imaizumi K; Araie M; Hara H. 2009. Effect of an inducer of BiP, a molecular chaperone, on endoplasmic reticulum (ER) stress-induced retinal cell death. Invest Ophthalmol Vis Sci 50(1):334-44. [PubMed: 18757512] [MGI Ref ID J:146693]
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a mixed B6CBAF1 background. The donating investigator has been backcrossing the strain to C57BL/6 and indicated that it was at N6 (4/14/00). Coat color expected from breeding:Black Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $239.00 Female or Male Hemizygous for Tg(Thy1-CFP)23Jrs
Pairs /Price (US dollars $) Pair Genotype $293.35 Hemizygous for Tg(Thy1-CFP)23Jrs x Noncarrier $293.35 Noncarrier x Hemizygous for Tg(Thy1-CFP)23Jrs
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $310.70 Female or Male Hemizygous for Tg(Thy1-CFP)23Jrs
Pairs /Price (US dollars $) Pair Genotype $381.40 Hemizygous for Tg(Thy1-CFP)23Jrs x Noncarrier $381.40 Noncarrier x Hemizygous for Tg(Thy1-CFP)23Jrs
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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