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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Background Strain C57BL/6J Donor Strain B6CBAF1 Generation N5+N22 (27-JAN-12)
Generation DefinitionsDonating Investigator Joshua Sanes, Harvard University Description
These mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. This line provides a strong and specific vital marker for axons; fluorescence is also stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. Availability of multiple spectral variants is useful for double-labeling applications, or breeding to other YFP/GFP lines.Development
A transgenic construct containing a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
004218 B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J 008513 B6.129P2-Gt(ROSA)26Sortm1(Trpv1,ECFP)Mde/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 014131 B6.Cg-Tg(Thy1-CFP)IJrs/GfngJ 007940 B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J 007967 B6.Cg-Tg(Thy1-CFP/COX8A)S2Lich/J 013161 B6.Cg-Tg(Thy1-Clomeleon)1Gjau/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 008004 B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J 007856 B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J 007879 STOCK Stx1atm2Sud/J 003773 STOCK Tg(CAG-ECFP)CK6Nagy/J 006784 STOCK Tg(Ins1-Cerulean)24Hara/J 016922 STOCK Tg(Myh7-CFP)1Jrs/J 013162 STOCK Tg(Thy1-Clomeleon)12Gjau/J 013163 STOCK Tg(Thy1-Clomeleon)13Gjau/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research
cell marker
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-CFP)23Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 23, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Thy-1-CFP; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6J x CBA)F1 | ||
| Site of Expression | High levels of expression in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in non-neural cells. | ||
| Expressed Gene | CFP, Cyan Fluorescent Protein, jellyfish | ||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Thy1-CFP)23Jrs, transgene insertion 23, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Thy-1-CFP; | ||
| General Note | Transgenic mice express a spectral variant of GFP (cyan-CFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. Fluorescence is stable to mild aldehyde fixation. Expression is strong from a mid-gestational stage into adulthood. | ||
| Molecular Note | The transgene contains a CFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. Lines 4, 11, 23, D, F, H, I, and S were established. [MGI Ref ID J:93699] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-CFP)23Jrs, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Tg(Thy1-CFP)23Jrs relatedBernstein SL; Guo Y; Slater BJ; Puche A; Kelman SE. 2007. Neuron stress and loss following rodent anterior ischemic optic neuropathy in double-reporter transgenic mice. Invest Ophthalmol Vis Sci 48(5):2304-10. [PubMed: 17460295] [MGI Ref ID J:123308]
Dratviman-Storobinsky O; Hasanreisoglu M; Offen D; Barhum Y; Weinberger D; Goldenberg-Cohen N. 2008. Progressive damage along the optic nerve following induction of crush injury or rodent anterior ischemic optic neuropathy in transgenic mice. Mol Vis 14:2171-9. [PubMed: 19052651] [MGI Ref ID J:143239]
Gastinger MJ; Kunselman AR; Conboy EE; Bronson SK; Barber AJ. 2008. Dendrite remodeling and other abnormalities in the retinal ganglion cells of Ins2 Akita diabetic mice. Invest Ophthalmol Vis Sci 49(6):2635-42. [PubMed: 18515593] [MGI Ref ID J:137045]
Howell GR; Libby RT; Jakobs TC; Smith RS; Phalan FC; Barter JW; Barbay JM; Marchant JK; Mahesh N; Porciatti V; Whitmore AV; Masland RH; John SW. 2007. Axons of retinal ganglion cells are insulted in the optic nerve early in DBA/2J glaucoma. J Cell Biol 179(7):1523-37. [PubMed: 18158332] [MGI Ref ID J:131073]
Inokuchi Y; Nakajima Y; Shimazawa M; Kurita T; Kubo M; Saito A; Sajiki H; Kudo T; Aihara M; Imaizumi K; Araie M; Hara H. 2009. Effect of an inducer of BiP, a molecular chaperone, on endoplasmic reticulum (ER) stress-induced retinal cell death. Invest Ophthalmol Vis Sci 50(1):334-44. [PubMed: 18757512] [MGI Ref ID J:146693]
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Wang X; Archibald ML; Stevens K; Baldridge WH; Chauhan BC. 2010. Cyan fluorescent protein (CFP) expressing cells in the retina of Thy1-CFP transgenic mice before and after optic nerve injury. Neurosci Lett 468(2):110-4. [PubMed: 19879331] [MGI Ref ID J:156897]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a mixed B6CBAF1 background. The donating investigator has been backcrossing the strain to C57BL/6 and indicated that it was at N6 (4/14/00). Coat color expected from breeding:Black Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $261.00 Female or Male Hemizygous for Tg(Thy1-CFP)23Jrs
Pairs /Price (US dollars $) Pair Genotype $321.00 Hemizygous for Tg(Thy1-CFP)23Jrs x Noncarrier $321.00 Noncarrier x Hemizygous for Tg(Thy1-CFP)23Jrs Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $339.30 Female or Male Hemizygous for Tg(Thy1-CFP)23Jrs
Pairs /Price (US dollars $) Pair Genotype $417.30 Hemizygous for Tg(Thy1-CFP)23Jrs x Noncarrier $417.30 Noncarrier x Hemizygous for Tg(Thy1-CFP)23Jrs Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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