Strain Name: |
FVB-Tg(GadGFP)45704Swn/J |
|---|---|
Stock Number: |
003718 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; Gad1; Tg(GadGFP)45704Swn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator John Swann, Baylor College of Med./Texas Child. Hosp Generation F?+N1F14N1F3 (04-DEC-07) Strain Description
Mice homozygous for the TgN(GadGFP)45704Swn transgene express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse Gad1 (GAD67) gene promoter. Homozygous mice exhibit no apparent physical or behavioral defects. Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin. This subset of interneurons has been shown to be prone to injury during epilepsy, ischemia, and Alzheimer's disease. These transgenic mice are useful for the morphological identification and study of these interneurons in both living and fixed brain tissue.Of note, this strain is one of many fluorescent GABAergic neuron strains, each with unique labeling characteristics (see Stock No. 006334 and Stock No. 006340).
Strain Development
A transgenic construct containing a 5' portion of the mouse Gad1 gene, a GFP open reading frame, and SV40 polyadenylation site sequence was injected into fertilized FVB/N mouse eggs. Founder animals were bred to wildtype FVB/NHsd mice. Homozygous mice were generated and interbred.
Gene & Allele Details
| Allele Symbol | Tg(GadGFP)45704Swn | ||
|---|---|---|---|
| Allele Name | transgene insertion 45704, John W Swann | ||
| Common Name(s) | GIN; | ||
| Mutation Made By | John Swann, Baylor College of Med./Texas Child. Hosp | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | Gad1, glutamic acid decarboxylase 1, mouse, laboratory | ||
| General Note | Homozygous transgenic mice exhibit no apparent physical or behavioral defects. Transgene expression occurs in a specific subpopulation of hippocampal and cortical GABAergic interneurons that express somatostatin. | ||
| Molecular Note | The transgene contains a 5' portion of the mouse Gad1 gene, a GFP open reading frame, and SV40 polyadenylation site sequence. Homozygous transgenic mice express Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse Gad1 gene promoter. [J:93867] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins
Colony Maintenance
| Breeding & Husbandry | The donating investigator maintains this strain a homozygote on a FVB background. Coat color expected from breeding:Albino |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
007677 CB6-Tg(Gad1-EGFP)G42Zjh/J 006334 STOCK Tg(Gad1-EGFP)94Agmo/J 006340 STOCK Tg(Gad1-EGFP)98Agmo/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX Notes, Fall 2002; 487. Green Fluorescent Protein (GFP) Transgenic Mice Poster Available.
JAX Notes, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedNeurobiology Research
Alzheimer's Disease
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesOliva AA Jr; Jiang M; Lam T; Smith KL; Swann JW. 2000. Novel hippocampal interneuronal subtypes identified using transgenic mice that express green fluorescent protein in GABAergic interneurons. J Neurosci 20(9):3354-68. [PubMed: 10777798] [J:93867]
Price and Supply Information
| Strain Name: | FVB-Tg(GadGFP)45704Swn/J |
| Stock Number: | 003718 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of mice by a company or other for profit entity will require a license from Baylor College of Medicine.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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