Strain Name: |
B6.129-Gabrdtm1Geh/J |
|---|---|
Stock Number: |
003725 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Gabrd; Gabrdtm1Geh; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Gregg Homanics, University of Pittsburgh Appearance
black
Related Genotype: a/aStrain Description
Mice that are homozygous null for Gabrd are viable and fertile. Northern blot analysis of -/- adult cerebellar RNA suggests that a chimeric Gabrd /neo transcript is generated from the targeted allele. Western blot analysis fails to detect a Gabrd protein product. Maximal binding for the agonist muscimol was markedly decreased in whole brain homogenates. Consistent with this, in situ autoradiographic analysis indicates a drastic reduction in muscimol binding in most parts of the brain. In general, these mice exhibit a decreased sensitivity to the sedative/hypnotic, anxiolytic and pro-absence effects of neuroactive steroids as determined by sleep time assays, elevated plus-maze assays (measures anxiolytic effect) and the inability of neuroactive steroids to prolong pentylenetetrazol-induced seizures. This decreased sensitivity appears to be selective however, as differential responses to various neuroactive steroids are observed.Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Gabrdtm1Geh | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Gregg E Homanics | ||
| Common Name(s) | delta-; | ||
| Mutation Made By | Gregg Homanics, University of Pittsburgh | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Gene Symbol and Name | Gabrd, gamma-aminobutyric acid (GABA-A) receptor, subunit delta | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | AI853201; GABAA-RD; MGC105467; MGC45284; expressed sequence AI853201; | ||
| Molecular Note | Insertion of a MC1-neomycin resistance cassette into exon 4 disrupted the gene. Northern blot analysis of cerebellar total RNA from homozygous mutant adult mice detected moderate levels of a novel 2.3kb transcript but not the 1.9kb wild type transcript. Western blot analysis of cerebellar membranes did not detect the wild type protein in homozygous mutant mice. [MGI Ref ID J:64288] | ||
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Gabrdtm1Geh
NEOTD (Generic Neo)
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Congenic Nomenclature
Gabrdtm1Geh relatedNeurobiology Research
Receptor Defects (GABA receptor)
Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects
Selected Reference(s)
Additional ReferencesMihalek RM; Banerjee PK; Korpi ER; Quinlan JJ; Firestone LL; Mi ZP; Lagenaur C; Tretter V; Sieghart W; Anagnostaras SG; Sage JR; Fanselow MS; Guidotti A; Spigelman I; Li Z; DeLorey TM; Olsen RW; Homanics GE. 1999. Attenuated sensitivity to neuroactive steroids in gamma-aminobutyrate type A receptor delta subunit knockout mice. Proc Natl Acad Sci U S A 96(22):12905-10. [PubMed: 10536021] [MGI Ref ID J:64288]
| Strain Name: | B6.129-Gabrdtm1Geh/J |
| Stock Number: | 003725 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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