Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male)   01-MAR-06 Species laboratory mouse Generation N4+N4F11 (28-DEC-12) Generation Definitions

Description
Mice that are homozygous null for Cd14 are viable and fertile. No Cd14 protein product is detected in thioglycollate elicited peritoneal (T-EP) macrophages by Western blot analysis. Unlike wildtype T-EP macrophages, T-EP macrophages derived from these animals fail to secrete TNF-alpha and IL-6 in response to lipopolysaccharide (LPS). Such a response is observed when Cd14 -null T-EP macrophages are exposed to whole bacteria (in the form of E. coli bioparticles), apparently by a Cd14-independent mechanism.

Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the entire Cd14 coding region, 4.0 kb of upstream sequence and 2.5 kb of downstream sequence. The construct was transfected into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6J females.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

JAX^® NOTES, Summer 2005; 498. Toll-like Receptor JAX^® Mice for Immunological Research.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Cd14^tm1Frm/Cd14^tm1Frm

        B6.129S4-Cd14^tm1Frm

• respiratory system phenotype
• pulmonary edema
  • less protein leakage into bronchoalveolar lavage fluid after lipopolysaccharide inhalation   (MGI Ref ID J:96680)
• immune system phenotype
• abnormal cytokine level
  • keratinocyte derived cytokine levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
  • macrophage inflammatory protein-2 levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
• • abnormal interleukin level
    • Il-6 levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
  • abnormal tumor necrosis factor level
    • TNF alpha levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
• skeleton phenotype
• decreased susceptibility to bone fracture
  • bones resistant to fracture   (MGI Ref ID J:118467)
• increased bone mass
  • increased bone area of the tibia   (MGI Ref ID J:118467)
• increased bone mineral content
  • greater mineral content   (MGI Ref ID J:118467)
• increased bone mineral density   (MGI Ref ID J:118467)
• homeostasis/metabolism phenotype
• abnormal cytokine level
  • keratinocyte derived cytokine levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
  • macrophage inflammatory protein-2 levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
• • abnormal interleukin level
    • Il-6 levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
  • abnormal tumor necrosis factor level
    • TNF alpha levels in lungs are unaffected by low doses of lipopolysaccharide   (MGI Ref ID J:96680)
• pulmonary edema
  • less protein leakage into bronchoalveolar lavage fluid after lipopolysaccharide inhalation   (MGI Ref ID J:96680)
• adipose tissue phenotype
• decreased total body fat amount   (MGI Ref ID J:118467)
• growth/size phenotype
• increased lean body mass   (MGI Ref ID J:118467)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd14^tm1Frm/Cd14⁺

        involves: 129S4/SvJae * C57BL/6J

• immune system phenotype
• abnormal cytokine secretion
  • macrophages exhibited a decrease in cytokine production in response to LPS   (MGI Ref ID J:65107)
• • decreased interleukin-6 secretion
    • decreased IL-6 production by macrophages in response to LPS   (MGI Ref ID J:65107)
  • decreased tumor necrosis factor secretion
    • decreased TNF production by macrophages in response to LPS   (MGI Ref ID J:65107)

Cd14^tm1Frm/Cd14^tm1Frm

        involves: 129S4/SvJae * C57BL/6J

• immune system phenotype
• abnormal cytokine secretion
  • macrophages exhibited loss of cytokine production in response to LPS and low levels of heat-killed E.coli (less than 1 E.coli per cell), however macrophages stimulated with high levels (10 E.coli per cell) of heat-killed E.coli, produced cytokine levels similar to wild-type while macrophages stimulated with lower levels (less than 10 E.coli per cell) produced reduced cytokine levels   (MGI Ref ID J:65107)
• • decreased interleukin-6 secretion
    • no IL-6 production by macrophages in response to LPS from E.coli K235   (MGI Ref ID J:65107)
  • decreased tumor necrosis factor secretion
    • no TNF-alpha production by macrophages in response to LPS from E.coli K235 or to low levels of heat-killed E.coli (less than 1 E.coli per cell)   (MGI Ref ID J:65107)

Cd14^tm1Frm/Cd14^tm1Frm

        involves: 129S4/SvJae

• immune system phenotype
• abnormal interferon secretion
  • exhibited no type I interferon production in response to smooth LPS   (MGI Ref ID J:98908)
• decreased susceptibility to endotoxin shock
  • exhibited no TNF production in response to smooth lipopolysaccharide (LPS), however response to lipid A from Salmonella Minnesota was normal   (MGI Ref ID J:98908)
• increased susceptibility to viral infection
  • macrophages exposed to vesicular stomatitis virus (VSV) could not contain the infection and were more susceptible to lysis than controls   (MGI Ref ID J:98908)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cd14^tm1Frm related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      genes regulating susceptibility to infectious disease and endotoxin

Research Tools
Immunology and Inflammation Research
      genes regulating susceptibility to infectious disease and endotoxin

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Cd14tm1Frm
Allele Name		targeted mutation 1, Mason W Freeman
Allele Type		Targeted (knock-out)
Common Name(s)		CD14-;
Mutation Made By		Mason Freeman,   Massachusetts General Hospital
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Cd14, CD14 antigen
Chromosome		18
Molecular Note		A neomycin resistance cassette replaced a genomic fragment containing the entire coding sequences of the gene. Western blot analysis confirmed an absence of CD14 protein in macrophage lysates derived from homozygous mice. [MGI Ref ID J:65107]

Genotyping

Genotyping Information

Genotyping Protocols

Cd14^tm1Frm, Melt Curve Analysis
Cd14^tm1Frm, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Moore KJ; Andersson LP; Ingalls RR; Monks BG; Li R; Arnaout MA; Golenbock DT; Freeman MW. 2000. Divergent response to LPS and bacteria in CD14-deficient murine macrophages J Immunol 165(8):4272-80. [PubMed: 11035061]  [MGI Ref ID J:65107]

Additional References

Dunzendorfer S; Lee HK; Soldau K; Tobias PS. 2004. TLR4 is the signaling but not the lipopolysaccharide uptake receptor. J Immunol 173(2):1166-70. [PubMed: 15240706]  [MGI Ref ID J:91928]

Cd14^tm1Frm related

Alugupalli KR; Akira S; Lien E; Leong JM. 2007. MyD88- and Bruton's tyrosine kinase-mediated signals are essential for T cell-independent pathogen-specific IgM responses. J Immunol 178(6):3740-9. [PubMed: 17339472]  [MGI Ref ID J:144277]

Anas AA; Hovius JW; van 't Veer C; van der Poll T; de Vos AF. 2010. Role of CD14 in a mouse model of acute lung inflammation induced by different lipopolysaccharide chemotypes. PLoS One 5(4):e10183. [PubMed: 20419140]  [MGI Ref ID J:160142]

Baumann CL; Aspalter IM; Sharif O; Pichlmair A; Bluml S; Grebien F; Bruckner M; Pasierbek P; Aumayr K; Planyavsky M; Bennett KL; Colinge J; Knapp S; Superti-Furga G. 2010. CD14 is a coreceptor of Toll-like receptors 7 and 9. J Exp Med 207(12):2689-701. [PubMed: 21078886]  [MGI Ref ID J:176872]

Bjorkbacka H; Kunjathoor VV; Moore KJ; Koehn S; Ordija CM; Lee MA; Means T; Halmen K; Luster AD; Golenbock DT; Freeman MW. 2004. Reduced atherosclerosis in MyD88-null mice links elevated serum cholesterol levels to activation of innate immunity signaling pathways. Nat Med 10(4):416-21. [PubMed: 15034566]  [MGI Ref ID J:90091]

Brandhorst TT; Wuthrich M; Finkel-Jimenez B; Warner T; Klein BS. 2004. Exploiting type 3 complement receptor for TNF-alpha suppression, immune evasion, and progressive pulmonary fungal infection. J Immunol 173(12):7444-53. [PubMed: 15585870]  [MGI Ref ID J:94857]

Cai S; Zemans RL; Young SK; Worthen GS; Jeyaseelan S. 2009. Myeloid differentiation protein-2-dependent and -independent neutrophil accumulation during Escherichia coli pneumonia. Am J Respir Cell Mol Biol 40(6):701-9. [PubMed: 18988922]  [MGI Ref ID J:160863]

Cani PD; Amar J; Iglesias MA; Poggi M; Knauf C; Bastelica D; Neyrinck AM; Fava F; Tuohy KM; Chabo C; Waget A; Delmee E; Cousin B; Sulpice T; Chamontin B; Ferrieres J; Tanti JF; Gibson GR; Casteilla L; Delzenne NM; Alessi MC; Burcelin R. 2007. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes 56(7):1761-72. [PubMed: 17456850]  [MGI Ref ID J:126459]

Cani PD; Bibiloni R; Knauf C; Waget A; Neyrinck AM; Delzenne NM; Burcelin R. 2008. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet-induced obesity and diabetes in mice. Diabetes 57(6):1470-81. [PubMed: 18305141]  [MGI Ref ID J:136880]

Cao L; Tanga FY; Deleo JA. 2009. The contributing role of CD14 in toll-like receptor 4 dependent neuropathic pain. Neuroscience 158(2):896-903. [PubMed: 18976692]  [MGI Ref ID J:145770]

Chase JC; Bosio CM. 2010. The presence of CD14 overcomes evasion of innate immune responses by virulent Francisella tularensis in human dendritic cells in vitro and pulmonary cells in vivo. Infect Immun 78(1):154-67. [PubMed: 19841074]  [MGI Ref ID J:155703]

Cho K; Thomas RL; Greenhalgh DG. 2003. CD14-dependent regulation of Grp78 in the liver and lungs of mice after burn injury. Exp Mol Pathol 75(2):148-53. [PubMed: 14516777]  [MGI Ref ID J:105838]

Davey M; Liu X; Ukai T; Jain V; Gudino C; Gibson FC 3rd; Golenbock D; Visintin A; Genco CA. 2008. Bacterial fimbriae stimulate proinflammatory activation in the endothelium through distinct TLRs. J Immunol 180(4):2187-95. [PubMed: 18250425]  [MGI Ref ID J:132003]

Dessing MC; Knapp S; Florquin S; de Vos AF; van der Poll T. 2007. CD14 facilitates invasive respiratory tract infection by Streptococcus pneumoniae. Am J Respir Crit Care Med 175(6):604-11. [PubMed: 17185649]  [MGI Ref ID J:135911]

Dessing MC; van der Sluijs KF; Florquin S; van der Poll T. 2007. CD14 plays a limited role during influenza A virus infection in vivo. Immunol Lett 113(1):47-51. [PubMed: 17825924]  [MGI Ref ID J:145233]

Echchannaoui H; Frei K; Letiembre M; Strieter RM; Adachi Y; Landmann R. 2005. CD14 deficiency leads to increased MIP-2 production, CXCR2 expression, neutrophil transmigration, and early death in pneumococcal infection. J Leukoc Biol 78(3):705-15. [PubMed: 15941778]  [MGI Ref ID J:100487]

Ehrentraut SF; Dorr A; Ehrentraut H; Lohner R; Lee SH; Hoeft A; Baumgarten G; Knuefermann P; Boehm O; Meyer R. 2012. Vascular dysfunction following polymicrobial sepsis: role of pattern recognition receptors. PLoS One 7(9):e44531. [PubMed: 22970242]  [MGI Ref ID J:191885]

Esen N; Kielian T. 2005. Recognition of Staphylococcus aureus-derived peptidoglycan (PGN) but not intact bacteria is mediated by CD14 in microglia. J Neuroimmunol 170(1-2):93-104. [PubMed: 16229899]  [MGI Ref ID J:106386]

Good DW; George T; Watts BA 3rd. 2012. Toll-like receptor 2 is required for LPS-induced Toll-like receptor 4 signaling and inhibition of ion transport in renal thick ascending limb. J Biol Chem 287(24):20208-20. [PubMed: 22523073]  [MGI Ref ID J:186513]

Guo S; Al-Sadi R; Said HM; Ma TY. 2013. Lipopolysaccharide Causes an Increase in Intestinal Tight Junction Permeability in Vitro and in Vivo by Inducing Enterocyte Membrane Expression and Localization of TLR-4 and CD14. Am J Pathol 182(2):375-87. [PubMed: 23201091]  [MGI Ref ID J:192566]

Hasebe A; Mu HH; Washburn LR; Chan FV; Pennock ND; Taylor ML; Cole BC. 2007. Inflammatory lipoproteins purified from a toxigenic and arthritogenic strain of Mycoplasma arthritidis are dependent on Toll-like receptor 2 and CD14. Infect Immun 75(4):1820-6. [PubMed: 17283106]  [MGI Ref ID J:119762]

Hassan F; Ren D; Zhang W; Merkel TJ; Gu XX. 2012. Moraxella catarrhalis activates murine macrophages through multiple toll like receptors and has reduced clearance in lungs from TLR4 mutant mice. PLoS One 7(5):e37610. [PubMed: 22662179]  [MGI Ref ID J:187305]

Henneke P; Takeuchi O; Malley R; Lien E; Ingalls RR; Freeman MW; Mayadas T; Nizet V; Akira S; Kasper DL; Golenbock DT. 2002. Cellular activation, phagocytosis, and bactericidal activity against group B streptococcus involve parallel myeloid differentiation factor 88-dependent and independent signaling pathways. J Immunol 169(7):3970-7. [PubMed: 12244198]  [MGI Ref ID J:120215]

Isayama F; Hines IN; Kremer M; Milton RJ; Byrd CL; Perry AW; McKim SE; Parsons C; Rippe RA; Wheeler MD. 2006. LPS signaling enhances hepatic fibrogenesis caused by experimental cholestasis in mice. Am J Physiol Gastrointest Liver Physiol 290(6):G1318-28. [PubMed: 16439470]  [MGI Ref ID J:111092]

Jacque B; Stephan K; Smirnova I; Kim B; Gilling D; Poltorak A. 2006. Mice expressing high levels of soluble CD14 retain LPS in the circulation and are resistant to LPS-induced lethality. Eur J Immunol 36(11):3007-16. [PubMed: 17039565]  [MGI Ref ID J:116991]

Janot L; Sirard JC; Secher T; Noulin N; Fick L; Akira S; Uematsu S; Didierlaurent A; Hussell T; Ryffel B; Erard F. 2009. Radioresistant cells expressing TLR5 control the respiratory epithelium's innate immune responses to flagellin. Eur J Immunol 39(6):1587-96. [PubMed: 19424969]  [MGI Ref ID J:149549]

Jeyaseelan S; Chu HW; Young SK; Freeman MW; Worthen GS. 2005. Distinct roles of pattern recognition receptors CD14 and Toll-like receptor 4 in acute lung injury. Infect Immun 73(3):1754-63. [PubMed: 15731076]  [MGI Ref ID J:96680]

Jiang Z; Georgel P; Du X; Shamel L; Sovath S; Mudd S; Huber M; Kalis C; Keck S; Galanos C; Freudenberg M; Beutler B. 2005. CD14 is required for MyD88-independent LPS signaling. Nat Immunol 6(6):565-70. [PubMed: 15895089]  [MGI Ref ID J:98908]

Johnson GB; Riggs BL; Platt JL. 2004. A genetic basis for the 'Adonis' phenotype of low adiposity and strong bones. FASEB J 18(11):1282-4. [PubMed: 15208271]  [MGI Ref ID J:118467]

Keck S; Muller I; Fejer G; Savic I; Tchaptchet S; Nielsen PJ; Galanos C; Huber M; Freudenberg MA. 2011. Absence of TRIF signaling in lipopolysaccharide-stimulated murine mast cells. J Immunol 186(9):5478-88. [PubMed: 21441453]  [MGI Ref ID J:173118]

Kerfoot SM; Long EM; Hickey MJ; Andonegui G; Lapointe BM; Zanardo RC; Bonder C; James WG; Robbins SM; Kubes P. 2004. TLR4 contributes to disease-inducing mechanisms resulting in central nervous system autoimmune disease. J Immunol 173(11):7070-7. [PubMed: 15557205]  [MGI Ref ID J:94363]

Knapp S; Matt U; Leitinger N; van der Poll T. 2007. Oxidized phospholipids inhibit phagocytosis and impair outcome in gram-negative sepsis in vivo. J Immunol 178(2):993-1001. [PubMed: 17202362]  [MGI Ref ID J:142624]

Knapp S; von Aulock S; Leendertse M; Haslinger I; Draing C; Golenbock DT; van der Poll T. 2008. Lipoteichoic Acid-Induced Lung Inflammation Depends on TLR2 and the Concerted Action of TLR4 and the Platelet-Activating Factor Receptor. J Immunol 180(5):3478-84. [PubMed: 18292574]  [MGI Ref ID J:131521]

Lee HK; Dunzendorfer S; Soldau K; Tobias PS. 2006. Double-stranded RNA-mediated TLR3 activation is enhanced by CD14. Immunity 24(2):153-63. [PubMed: 16473828]  [MGI Ref ID J:113319]

Leendertse M; Willems RJ; Giebelen IA; van den Pangaart PS; Wiersinga WJ; de Vos AF; Florquin S; Bonten MJ; van der Poll T. 2008. TLR2-Dependent MyD88 Signaling Contributes to Early Host Defense in Murine Enterococcus faecium Peritonitis. J Immunol 180(7):4865-74. [PubMed: 18354210]  [MGI Ref ID J:133374]

Liu H; Fitzgerald D; Gran B; Leong JM; Alugupalli KR. 2010. Induction of distinct neurologic disease manifestations during relapsing fever requires T lymphocytes. J Immunol 184(10):5859-64. [PubMed: 20382883]  [MGI Ref ID J:161007]

McAvoy EF; McDonald B; Parsons SA; Wong CH; Landmann R; Kubes P. 2011. The role of CD14 in neutrophil recruitment within the liver microcirculation during endotoxemia. J Immunol 186(4):2592-601. [PubMed: 21217012]  [MGI Ref ID J:169174]

Montminy SW; Khan N; McGrath S; Walkowicz MJ; Sharp F; Conlon JE; Fukase K; Kusumoto S; Sweet C; Miyake K; Akira S; Cotter RJ; Goguen JD; Lien E. 2006. Virulence factors of Yersinia pestis are overcome by a strong lipopolysaccharide response. Nat Immunol 7(10):1066-73. [PubMed: 16980981]  [MGI Ref ID J:112661]

Moore KJ; El Khoury J; Medeiros LA; Terada K; Geula C; Luster AD; Freeman MW. 2002. A CD36-initiated signaling cascade mediates inflammatory effects of beta-amyloid. J Biol Chem 277(49):47373-9. [PubMed: 12239221]  [MGI Ref ID J:80658]

Muthu K; He LK; Szilagyi A; Strotmon P; Gamelli RL; Shankar R. 2010. ss-adrenergic stimulation increases macrophage CD14 expression and E. coli phagocytosis through PKA signaling mechanisms. J Leukoc Biol 88(4):715-24. [PubMed: 20643814]  [MGI Ref ID J:165610]

Oakley MS; Majam V; Mahajan B; Gerald N; Anantharaman V; Ward JM; Faucette LJ; McCutchan TF; Zheng H; Terabe M; Berzofsky JA; Aravind L; Kumar S. 2009. Pathogenic roles of CD14, galectin-3, and OX40 during experimental cerebral malaria in mice. PLoS One 4(8):e6793. [PubMed: 19710907]  [MGI Ref ID J:152399]

Pawlik TM; Carter EA; Bode BP; Fischman AJ; Tompkins RG. 2003. Central role of interleukin-6 in burn induced stimulation of hepatic amino acid transport. Int J Mol Med 12(4):541-8. [PubMed: 12964032]  [MGI Ref ID J:113636]

Pihlgren M; Silva AB; Madani R; Giriens V; Waeckerle-Men Y; Fettelschoss A; Hickman DT; Lopez-Deber MP; Ndao DM; Vukicevic M; Buccarello AL; Gafner V; Chuard N; Reis P; Piorkowska K; Pfeifer A; Kundig TM; Muhs A; Johansen P. 2013. TLR4- and TRIF-dependent stimulation of B lymphocytes by peptide liposomes enables T cell-independent isotype switch in mice. Blood 121(1):85-94. [PubMed: 23144170]  [MGI Ref ID J:192819]

Reed-Geaghan EG; Reed QW; Cramer PE; Landreth GE. 2010. Deletion of CD14 attenuates Alzheimer's disease pathology by influencing the brain's inflammatory milieu. J Neurosci 30(46):15369-73. [PubMed: 21084593]  [MGI Ref ID J:166445]

Scott MJ; Billiar TR. 2008. Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes. J Biol Chem 283(43):29433-46. [PubMed: 18701460]  [MGI Ref ID J:142561]

Scott P; Ma H; Viriyakosol S; Terkeltaub R; Liu-Bryan R. 2006. Engagement of CD14 mediates the inflammatory potential of monosodium urate crystals. J Immunol 177(9):6370-8. [PubMed: 17056568]  [MGI Ref ID J:140511]

Seimon TA; Obstfeld A; Moore KJ; Golenbock DT; Tabas I. 2006. Combinatorial pattern recognition receptor signaling alters the balance of life and death in macrophages. Proc Natl Acad Sci U S A 103(52):19794-9. [PubMed: 17167049]  [MGI Ref ID J:118240]

Shao H; Lee S; Gae-Scott S; Nakata C; Chen S; Hamad AR; Chakravarti S. 2012. Extracellular matrix lumican promotes bacterial phagocytosis, and Lum-/- mice show increased Pseudomonas aeruginosa lung infection severity. J Biol Chem 287(43):35860-72. [PubMed: 22865855]  [MGI Ref ID J:191593]

Shinya K; Ito M; Makino A; Tanaka M; Miyake K; Eisfeld AJ; Kawaoka Y. 2012. The TLR4-TRIF Pathway Protects against H5N1 Influenza Virus Infection. J Virol 86(1):19-24. [PubMed: 22031950]  [MGI Ref ID J:179408]

Smoak KA; Aloor JJ; Madenspacher J; Merrick BA; Collins JB; Zhu X; Cavigiolio G; Oda MN; Parks JS; Fessler MB. 2010. Myeloid differentiation primary response protein 88 couples reverse cholesterol transport to inflammation. Cell Metab 11(6):493-502. [PubMed: 20519121]  [MGI Ref ID J:160911]

Stewart CR; Stuart LM; Wilkinson K; van Gils JM; Deng J; Halle A; Rayner KJ; Boyer L; Zhong R; Frazier WA; Lacy-Hulbert A; Khoury JE; Golenbock DT; Moore KJ. 2010. CD36 ligands promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer. Nat Immunol 11(2):155-61. [PubMed: 20037584]  [MGI Ref ID J:158401]

Togbe D; Schofield L; Grau GE; Schnyder B; Boissay V; Charron S; Rose S; Beutler B; Quesniaux VF; Ryffel B. 2007. Murine cerebral malaria development is independent of toll-like receptor signaling. Am J Pathol 170(5):1640-8. [PubMed: 17456769]  [MGI Ref ID J:121070]

Tsung A; Sahai R; Tanaka H; Nakao A; Fink MP; Lotze MT; Yang H; Li J; Tracey KJ; Geller DA; Billiar TR. 2005. The nuclear factor HMGB1 mediates hepatic injury after murine liver ischemia-reperfusion. J Exp Med 201(7):1135-43. [PubMed: 15795240]  [MGI Ref ID J:98036]

Viriyakosol S; Matthias MA; Swancutt MA; Kirkland TN; Vinetz JM. 2006. Toll-like receptor 4 protects against lethal Leptospira interrogans serovar icterohaemorrhagiae infection and contributes to in vivo control of leptospiral burden. Infect Immun 74(2):887-95. [PubMed: 16428731]  [MGI Ref ID J:105019]

Wieland CW; Florquin S; Maris NA; Hoebe K; Beutler B; Takeda K; Akira S; van der Poll T. 2005. The MyD88-dependent, but not the MyD88-independent, pathway of TLR4 signaling is important in clearing nontypeable haemophilus influenzae from the mouse lung. J Immunol 175(9):6042-9. [PubMed: 16237099]  [MGI Ref ID J:119385]

Wieland CW; van der Windt GJ; Wiersinga WJ; Florquin S; van der Poll T. 2008. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology 125(2):272-9. [PubMed: 18393969]  [MGI Ref ID J:143813]

Wilkinson TS; Dhaliwal K; Hamilton TW; Lipka AF; Farrell L; Davidson DJ; Duffin R; Morris AC; Haslett C; Govan JR; Gregory CD; Sallenave JM; Simpson AJ. 2009. Trappin-2 promotes early clearance of Pseudomonas aeruginosa through CD14-dependent macrophage activation and neutrophil recruitment. Am J Pathol 174(4):1338-46. [PubMed: 19264904]  [MGI Ref ID J:147088]

Yang KK; Dorner BG; Merkel U; Ryffel B; Schutt C; Golenbock D; Freeman MW; Jack RS. 2002. Neutrophil influx in response to a peritoneal infection with Salmonella is delayed in lipopolysaccharide-binding protein or CD14-deficient mice. J Immunol 169(8):4475-80. [PubMed: 12370383]  [MGI Ref ID J:79487]

Yauch LE; Mansour MK; Shoham S; Rottman JB; Levitz SM. 2004. Involvement of CD14, toll-like receptors 2 and 4, and MyD88 in the host response to the fungal pathogen Cryptococcus neoformans in vivo. Infect Immun 72(9):5373-82. [PubMed: 15322035]  [MGI Ref ID J:92432]

Zang QS; Maass DL; Wigginton JG; Barber RC; Martinez B; Idris AH; Horton JW; Nwariaku FE. 2010. Burn serum causes a CD14-dependent mitochondrial damage in primary cardiomyocytes. Am J Physiol Heart Circ Physiol 298(6):H1951-8. [PubMed: 20348223]  [MGI Ref ID J:160471]

Zanoni I; Ostuni R; Capuano G; Collini M; Caccia M; Ronchi AE; Rocchetti M; Mingozzi F; Foti M; Chirico G; Costa B; Zaza A; Ricciardi-Castagnoli P; Granucci F. 2009. CD14 regulates the dendritic cell life cycle after LPS exposure through NFAT activation. Nature 460(7252):264-8. [PubMed: 19525933]  [MGI Ref ID J:150351]

Zhao W; Beers DR; Henkel JS; Zhang W; Urushitani M; Julien JP; Appel SH. 2010. Extracellular mutant SOD1 induces microglial-mediated motoneuron injury. Glia 58(2):231-43. [PubMed: 19672969]  [MGI Ref ID J:159347]

Zhou H; Lapointe BM; Clark SR; Zbytnuik L; Kubes P. 2006. A requirement for microglial TLR4 in leukocyte recruitment into brain in response to lipopolysaccharide. J Immunol 177(11):8103-10. [PubMed: 17114485]  [MGI Ref ID J:140683]

Zhou S; Kurt-Jones EA; Mandell L; Cerny A; Chan M; Golenbock DT; Finberg RW. 2005. MyD88 is critical for the development of innate and adaptive immunity during acute lymphocytic choriomeningitis virus infection. Eur J Immunol 35(3):822-30. [PubMed: 15724245]  [MGI Ref ID J:96733]

von Meyenburg C; Hrupka BH; Arsenijevic D; Schwartz GJ; Landmann R; Langhans W. 2004. Role for CD14, TLR2, and TLR4 in bacterial product-induced anorexia. Am J Physiol Regul Integr Comp Physiol 287(2):R298-305. [PubMed: 15271679]  [MGI Ref ID J:109286]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129S background and is currently being backcrossed to C57BL/6J. Coat color expected from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.