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Genes & Alleles

Cd14;   Cd14tm1Frm;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N4+N3F15N1F2 (19-DEC-07)

Strain Description
Mice that are homozygous null for Cd14 are viable and fertile. No Cd14 protein product is detected in thioglycollate elicited peritoneal (T-EP) macrophages by Western blot analysis. Unlike wildtype T-EP macrophages, T-EP macrophages derived from these animals fail to secrete TNF-alpha and IL-6 in response to lipopolysaccharide (LPS). Such a response is observed when Cd14 -null T-EP macrophages are exposed to whole bacteria (in the form of E. coli bioparticles), apparently by a Cd14-independent mechanism.

Strain Development
A targeting vector containing neomycin resistance and thymidine kinase genes was used to disrupt the entire Cd14 coding region, 4.0 kb of upstream sequence and 2.5 kb of downstream sequence. The construct was transfected into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6J females.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. Cd14tm1Frm/Cd14+         involves: 129S4/SvJae * C57BL/6J immune system phenotype abnormal cytokine secretion (MGI Ref ID J:65107) macrophages exhibited a decrease in cytokine production in response to LPS decreased interleukin-6 secretion (MGI Ref ID J:65107) decreased IL-6 production by macrophages in response to LPS decreased tumor necrosis factor secretion (MGI Ref ID J:65107) decreased TNF production by macrophages in response to LPS Cd14tm1Frm/Cd14tm1Frm         involves: 129S4/SvJae * C57BL/6J immune system phenotype abnormal cytokine secretion (MGI Ref ID J:65107) macrophages exhibited loss of cytokine production in response to LPS and low levels of heat-killed E.coli (less than 1 E.coli per cell), however macrophages stimulated with high levels (10 E.coli per cell) of heat-killed E.coli, produced cytokine levels similar to wild type while macrophages stimulated with lower levels (less than 10 E.coli per cell) produced reduced cytokine levels decreased interleukin-6 secretion (MGI Ref ID J:65107) no IL-6 production by macrophages in response to LPS from E.coli K235 decreased tumor necrosis factor secretion (MGI Ref ID J:65107) no TNF-alpha production by macrophages in response to LPS from E.coli K235 or to low levels of heat-killed E.coli (less than 1 E.coli per cell) Cd14tm1Frm/Cd14tm1Frm         involves: 129S4/SvJae immune system phenotype abnormal interferon secretion (MGI Ref ID J:98908) exhibited no type I interferon production in response to smooth LPS decreased susceptibility to endotoxin shock (MGI Ref ID J:98908) exhibited no TNF production in response to smooth lipopolysaccharide (LPS), however response to lipid A from Salmonella Minnesota was normal increased susceptibility to viral infection (MGI Ref ID J:98908) macrophages exposed to vesicular stomatitis virus (VSV) could not contain the infection and were more susceptible to lysis than controls

Gene & Allele Details

Allele Symbol		Cd14tm1Frm
Allele Name		targeted mutation 1, Mason W Freeman
Common Name(s)		CD14-;
Mutation Made By		Mason Freeman,   Massachusetts General Hospital
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Cd14, CD14 antigen
Chromosome		18
Molecular Note		A neomycin resistance cassette replaced a genomic fragment containing the entire coding sequences of the gene. Western blot analysis confirmed an absence of CD14 protein in macrophage lysates derived from homozygous mice. [MGI Ref ID J:65107]

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Genotyping Protocols

Cd14^tm1Frm

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129S background and is currently being backcrossed to C57BL/6J. Coat color expected from breeding:Black
Diet Information	LabDiet® 5K52/5K67

Additional Web Information

Congenic Nomenclature
JAX^® NOTES, Summer 2005; 498. Toll-like Receptor JAX^® Mice for Immunological Research.

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Cd14^tm1Frm related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (genes regulating susceptibility to infectious disease and endotoxin)

Research Tools
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)

References

Selected Reference(s)

Moore KJ; Andersson LP; Ingalls RR; Monks BG; Li R; Arnaout MA; Golenbock DT; Freeman MW. 2000. Divergent response to LPS and bacteria in CD14-deficient murine macrophages J Immunol 165(8):4272-80. [PubMed: 11035061]  [MGI Ref ID J:65107]

Additional References

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Price and Supply Information

Strain Name:	B6.129S-Cd14tm1Frm/J
Stock Number:	003726

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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