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Strain Name:

NOD.129S7(B6)-Rag1tm1Mom/J

Stock Number:

003729

Availability:

Repository- Live

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Additional Licensing and Use Restrictions

Genes & Alleles   Rag1;   Rag1tm1Mom;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain NOD/LtSz
Donor Strain 129S7 via AB1 ES cell line
Donating Investigator Leonard Shultz,   The Jackson Laboratory
H2 Haplotypeg7
GenerationN10F?+F15N1F3 (04-DEC-07)

Appearance
albino
Related Genotype: Tyrc/Tyrc

Strain Description
Mice homozygous for the Rag1tm1Mom mutation produce no mature T cells or B cells. Their phenotype can be described as a "non-leaky" severe combined immune deficiency. (Prkdcscid/Prkdcscid) mice produce some B cells and IgM. NOD.129S7(B6)-Rag1tm1Mom/J mice have no CD3+ or T cell receptor (TCR) alpha-beta positive cells. The thymus of the mutant mice is severely involuted. The thymocytes are CD8-CD4- and most are IL2 receptor positive. Neither the spleen nor bone marrow contain any IgM or IgD staining cells, indicating an absence of mature B cells. These and other data suggest that both B cell and T cell development have been arrested at an early stage. Macroscopically, the mutants are indistinguishable from heterozygotes or normal wildtype siblings. Notable differences between this strain and the NOD.CB17-Prkdcscid/J strain (Stock No. 001303) include a longer life span due to delayed onset of lymphoma development, greater radioresistance and a complete absence of serum Ig. These characteristics qualify this strain as a useful tool for HIV-1 studies and research involving human hematolymphoid cell engraftment or NOD diabetes pathogenesis.

Strain Development
The Rag1tm1Mom mutant strain was developed by Dr. Peter Mombaerts in the laboratory of Dr. Susumu Tonegawa at the Center for Cancer Research, Massachusetts Institute of Technology. A replacement targeting vector with the Pgk-neo marker was used. Homologous recombination of the targeting vector resulted in a 1356 bp deletion in the 5' end of the coding sequence. The 129-derived AB1 ES cell line was used.

Mammalian Phenotype Terms assigned by genotype

Rag1tm1Mom/Rag1tm1Mom

        NOD.129S7(B6)-Rag1tm1Mom
  • immune system phenotype
  • abnormal level of surface class II molecules (MGI Ref ID J:109843)
    • four to five-fold decrease in cells expressing I-Ag7 as compared to NOD control
  • decreased CD4-positive T cell number (MGI Ref ID J:109843)
    • spleens of 8-11 week old mice are deficient in CD3+ CD4+ T cells
  • decreased CD8-positive T cell number (MGI Ref ID J:109843)
    • spleens of 8-11 week old mice are deficient in CD3+ CD8+ T cells
  • decreased immunoglobulin level (MGI Ref ID J:109843)
    • no serum immunoglobulin is detected in 218-334 day old mice
  • decreased mature B cell number (MGI Ref ID J:109843)
    • spleens of 8-11 week old mice are deficient in Igkappa+ B220+ T cells
  • impaired NK cell cytolysis (MGI Ref ID J:109843)
    • poly I:C stimulated spleen cells exhibit low levels of NK cell cyotoxic activity in comparison to C57BL/6 homozygotes
  • increased NK cell number (MGI Ref ID J:109843)
    • numbers of NK cells are increased in comparison to NOD controls
  • increased granulocyte number (MGI Ref ID J:109843)
    • numbers of granulocytes are increased in comparison to NOD controls
  • increased immature B cell number (MGI Ref ID J:109843)
    • IgLkappa- B220+ immature B cell numbers are increased as compared to NOD controls
  • increased macrophage cell number (MGI Ref ID J:109843)
    • numbers of macrophages are increased in comparison to NOD controls
  • spleen hypoplasia (MGI Ref ID J:109843)
    • nucleated spleen cells are reduced in 8-11 week old mice as compared to NOD control
  • hematopoietic system phenotype
  • decreased CD4-positive T cell number (MGI Ref ID J:109843)
    • spleens of 8-11 week old mice are deficient in CD3+ CD4+ T cells
  • decreased CD8-positive T cell number (MGI Ref ID J:109843)
    • spleens of 8-11 week old mice are deficient in CD3+ CD8+ T cells
  • decreased mature B cell number (MGI Ref ID J:109843)
    • spleens of 8-11 week old mice are deficient in Igkappa+ B220+ T cells
  • increased NK cell number (MGI Ref ID J:109843)
    • numbers of NK cells are increased in comparison to NOD controls
  • increased granulocyte number (MGI Ref ID J:109843)
    • numbers of granulocytes are increased in comparison to NOD controls
  • increased immature B cell number (MGI Ref ID J:109843)
    • IgLkappa- B220+ immature B cell numbers are increased as compared to NOD controls
  • increased macrophage cell number (MGI Ref ID J:109843)
    • numbers of macrophages are increased in comparison to NOD controls
  • spleen hypoplasia (MGI Ref ID J:109843)
    • nucleated spleen cells are reduced in 8-11 week old mice as compared to NOD control

Gene & Allele Details

Allele Symbol Rag1tm1Mom
Allele Name targeted mutation 1, Peter Mombaerts
Common Name(s) RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-;
Mutation Made By Peter Mombaerts,   Max Planck Institute of Biophysics
Strain of Origin129S7/SvEvBrd-Hprt1<+>
ES Cell Line NameAB1
ES Cell Line Strain129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name Rag1, recombination activating gene 1
Chromosome 2
Gene Common Name(s) MGC43321; RNF74; Rag-1;
Molecular Note A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [MGI Ref ID J:1934] [MGI Ref ID J:96036]

Control Information

  Allele   Control
 Rag1tm1Mom  001976 NOD/ShiLtJ
   Additional control strains are available depending on the researchers needs. Please refer to JAX Notes No. 477 for a complete list of control strains available for NOD/LtJ mice in diabetes research. JAX Notes .
 
  Considerations for Choosing Controls

Genotyping Protocols

Rag1tm1Mom

Colony Maintenance

Breeding & HusbandryThe original B6,129S-Rag1tm1Mom line was backcrossed to the NOD/LtSz strain background for ten generations. At the tenth generation, mice were intercrossed. They are now being maintained as homozygotes. These mice are severely immunodeficient so specific pathogen-free (SPF) conditions are recommended. Coat color expected from breeding: albino
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying   Rag1tm1Mom     (15 strains)

Strains carrying other alleles of Rag1
002194   B6;CBA-Tg(CD3E)26Cpt/J
002506   STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
View Strains carrying other alleles of Rag1     (2 strains)

Additional Web Information

Congenic Nomenclature
JAX® NOTES, Spring 2006; 501. Choosing an Immunodeficient Mouse Model.

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Islet Transplantation Studies
Type 1 Diabetes (IDDM) Analysis Strains (NOD Congenics with Mutations Affecting Immunocompetence)

Research Tools
Immunology and Inflammation Research (B and T cell deficiency)

Rag1tm1Mom related

Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)

Hematological Research
Immunological Defects (B and T cell deficiency)

Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)

Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)

Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)

References

Selected Reference(s)

Shultz LD; Lang PA; Christianson SW; Gott B; Lyons B; Umeda S; Leiter E; Hesselton R; Wagar EJ; Leif JH; Kollet O; Lapidot T; Greiner DL. 2000. NOD/LtSz-Rag1null mice: An immunodeficient and radioresistant model for engraftment of human hematolymphoid cells, HIV infection, and adoptive transfer of NOD mouse diabetogenic T cells J Immunol 164(5):2496-507. [PubMed: 10679087]  [MGI Ref ID J:60800]

Additional References

Price and Supply Information

Strain Name: NOD.129S7(B6)-Rag1tm1Mom/J
Stock Number: 003729

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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