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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;FVB-TgN(GZMB-Cre)1Jcb (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N3+1F4pN1 Donating Investigator Joshy Jacob, Emory University School of Medicine Description
This strain is one component of a Cre-recombinase mediated mouse model system that allows irreversible labeling of antigen-stimulated T cells with surface expressed ALPP (placental alkaline phosphatase). Cre recombinase is expressed under control of the human GZMB (granzyme B) promoter which directs specific expression in activated T cells. When this strain is bred with a Cre-recombinase expressing strain the loxP-flanked STOP fragment is excised. This strain represents an effective tool for generating tissue specific-targeted mutants.Development
A targeting construct containing the human granzyme B promoter, the SV40 nuclear localization signal sequence, Cre recombinase, and the human growth hormone polyadenylation sequence was injected into the pronuclei of fertilized FVB/N eggs. Founder mice were obtained and bred.
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(GZMB-cre)1Jcb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joshy Jacob | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | granzyme-B-Cre; | ||
| Mutation Made By | Joshy Jacob, Emory University School of Medicine | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | activated T cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | GZMB, granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), human | ||
| Driver Note | GZMB | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human granzyme B (GZMB) promoter, active specifically in activated T cells. [MGI Ref ID J:67937] | ||
| Gene Symbol and Name | Tg(GZMB-cre)1Jcb, transgene insertion 1, Joshy Jacob | ||
| Chromosome | UN | ||
| Gene Common Name(s) | granzyme-B-Cre; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Jacob J; Baltimore D. 1999. Modelling T-cell memory by genetic marking of memory T cells in vivo. Nature 399(6736):593-7. [PubMed: 10376601] [MGI Ref ID J:67937]
Additional References
Tg(GZMB-cre)1Jcb relatedChappell C; Beard C; Altman J; Jaenisch R; Jacob J. 2006. DNA methylation by DNA methyltransferase 1 is critical for effector CD8 T cell expansion. J Immunol 176(8):4562-72. [PubMed: 16585546] [MGI Ref ID J:131165]
Johnson TA; Tsutsui S; Jirik FR. 2008. Antigen-induced pten gene deletion in T cells exacerbates neuropathology in experimental autoimmune encephalomyelitis. Am J Pathol 172(4):980-92. [PubMed: 18349128] [MGI Ref ID J:133344]
Stranges PB; Watson J; Cooper CJ; Choisy-Rossi CM; Stonebraker AC; Beighton RA; Hartig H; Sundberg JP; Servick S; Kaufmann G; Fink PJ; Chervonsky AV. 2007. Elimination of antigen-presenting cells and autoreactive T cells by Fas contributes to prevention of autoimmunity. Immunity 26(5):629-41. [PubMed: 17509906] [MGI Ref ID J:123556]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This mutation was generated on a FVB/N background. Founder animals were backcrossed to C57BL/6 (N3 5/2000).
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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