Strain Name: |
B6;FVB-Tg(GZMB-cre)1Jcb/J |
|---|---|
Stock Number: |
003734 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Former Name |
B6;FVB-TgN(GZMB-Cre)1Jcb (Changed: 15-DEC-04
) |
| Genes & Alleles | GZMB; cre; Tg(GZMB-cre)1Jcb; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Strain Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Joshy Jacob, Emory University School of Medicine Generation N3+1F4pN1 Strain Description
This strain is one component of a Cre-recombinase mediated mouse model system that allows irreversible labeling of antigen-stimulated T cells with surface expressed ALPP (placental alkaline phosphatase). Cre recombinase is expressed under control of the human GZMB (granzyme B) promoter which directs specific expression in activated T cells. When this strain is bred with a Cre-recombinase expressing strain the loxP-flanked STOP fragment is excised. This strain represents an effective tool for generating tissue specific-targeted mutants.Strain Development
A targeting construct containing the human granzyme B promoter, the SV40 nuclear localization signal sequence, Cre recombinase, and the human growth hormone polyadenylation sequence was injected into the pronuclei of fertilized FVB/N eggs. Founder mice were obtained and bred.
Gene & Allele Details
| Allele Symbol | Tg(GZMB-cre)1Jcb | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Joshy Jacob | ||
| Common Name(s) | granzyme-B-Cre; | ||
| Mutation Made By | Joshy Jacob, Emory University School of Medicine | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | activated T cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | GZMB, granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1), human | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a human granzyme B (GZMB) promoter, active specifically in activated T cells. [MGI Ref ID J:67937] | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | This mutation was generated on a FVB/N background. Founder animals were backcrossed to C57BL/6 (N3 5/2000). |
|---|
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (116 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesJacob J; Baltimore D. 1999. Modelling T-cell memory by genetic marking of memory T cells in vivo. Nature 399(6736):593-7. [PubMed: 10376601] [MGI Ref ID J:67937]
Price and Supply Information
| Strain Name: | B6;FVB-Tg(GZMB-cre)1Jcb/J |
| Stock Number: | 003734 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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