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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6SJL-Tg(Prnp-MAPT)43Vle/J (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N6p (19-FEB-06)
Generation DefinitionsDonating Investigator Virginia M. Lee, University of Pennsylvania Description
These transgenic mice express the human fetal tau MAPT isoform under the direction of the mouse prion protein promoter. Hyperphosphorylated, insoluble MAPT protein is widely expressed in neurons of the CNS at levels approximately ten-fold higher than the endogenous mouse counterpart. Mice homozygous for the transgenic insert die at about three months of age. In the hemizygous mice intraneural inclusions that stain positive with T14, a monoclonal antibody specific for MAPT, are observed in brain and spinal cord tissue at 1 month of age. The number of inclusions increases until 6-9 months of age. Transmission electron microscopy studies of these inclusions reveals tightly packed aggregates of randomly arranged 10-20 nm straight filaments. Mice suffer progressive, age-dependant neuronal damage, motor weakness and gliosis. These mice recapitulate key features of tauopathies and provide a model for studying the underlying mechanism of related diseases such as FTDP-17, Alzheimer's and Pick's disease.Development
A transgenic construct containing the mouse Prnp (prion protein) promoter and a cDNA sequence to human MAPT (fetal tau, Tau44 isoform) was used to create transgenic animals on a B6D2F1 background. These mice were crossed with B6SJLF1 mice and then crossed with B6D2F1 mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Alzheimer's Disease Models
View Alzheimer's Disease Models (108 strains)
Additional Web Information
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Visit the Parkinson's Disease Resource site for helpful information on Parkinson's and research resources.
Phenotype
Phenotype Information
- Characteristics of this human disease are associated with transgenes and other mutation types in the mouse.
Alzheimer Disease; AD
Frontotemporal Dementia; FTD
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Parkinson Disease, Late-Onset; PD (MAPT)
Parkinson-Dementia Syndrome (MAPT)
Pick Disease of Brain (MAPT)
Supranuclear Palsy, Progressive, 1; PSNP1 (MAPT)
assigned by genotype
Tg(Prnp-MAPT)43Vle/0
involves: C57BL/6 * DBA/2
- nervous system phenotype
- abnormal axon morphology
- abnormal axonal transport
- fast axonal transport of proteins is retarded as compared to wildtype (MGI Ref ID J:61052)
- abnormal motor neuron morphology
- in addition to inclusions, vacuolar lesions are observed in older mice (MGI Ref ID J:61052)
- motor neuron degeneration (MGI Ref ID J:61052)
- astrocytosis
- detectable after one month of age (MGI Ref ID J:61052)
- axon degeneration
- tau protein deposits
- tau-positive spheroidal inclusions in spinal cord neurons are visible by 1 month of age (MGI Ref ID J:61052)
- size and number increases up to 6-9 months of age, but decreases in number by 12 months of age (MGI Ref ID J:61052)
- inclusions are the size of medium to large spinal cord neurons and some appear to arise within the proximal axons of spinal cord neurons (MGI Ref ID J:61052)
- spinal cord inclusions are most frequent at the grey-white matter junction (MGI Ref ID J:61052)
- spinal cord inclusions in line 43 are larger and more abundant than in line 7 (MGI Ref ID J:61052)
- tau-positive inclusions appear in cortical neurons and brainstem by 6 months of age (MGI Ref ID J:61052)
- inclusions in cortical neurons occur in proximal axons or somadendritic domains of neurons (MGI Ref ID J:61052)
- inclusions are visible in pontine neurons by 1 month of age (MGI Ref ID J:61052)
- tau deposits become increasingly insoluble with age (MGI Ref ID J:61052)
- behavior/neurological phenotype
- impaired balance
- impaired ability to stand on slanted surface (MGI Ref ID J:61052)
- limb grasping
- mice retract hindlimbs when lifted by the tail (MGI Ref ID J:61052)
- growth/size phenotype
- decreased body weight
- mice weigh 30-40% less than normal littermates (MGI Ref ID J:61052)
Tg(Prnp-MAPT)43Vle/Tg(Prnp-MAPT)43Vle
involves: C57BL/6 * DBA/2This mouse can be used to support research in many areas including:
MAPT relatedMouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Tg(Prnp-MAPT)43Vle relatedNeurobiology Research
Alzheimer's Disease
Parkinson's Disease
Neurobiology Research
Alzheimer's Disease
Tau (Mapt) mutants
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Prnp-MAPT)43Vle | ||
|---|---|---|---|
| Allele Name | transgene insertion 43, Virginia M Y Lee | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Virginia Lee, University of Pennsylvania | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Expressed Gene | MAPT, microtubule-associated protein tau, human | ||
| Promoter | Prnp, prion protein, mouse, laboratory | ||
| General Note | Three founder lines were generated - lines 7, 27 and 43. Hemizygous mice from line 27 are not viable beyond 3 months and were not characterized in J:61052. Hemizygous mice from line 7 are phenotypically similar to line 43, however inclusions in the spinal cord are smaller and less abundant than in line 43. | ||
| Molecular Note | A transgenic construct containing a cDNA sequence to human MAPT (fetal tau, Tau44 isoform) under the control of the mouse Prnp promtoer was used to create transgenic animals. Quantitative Western blot analysis showed hyperphosphorylated, insoluble MAPT protein to be widely expressed in neurons of the CNS at levels approximately 10 fold higher than the endogenous mouse counterpart. [MGI Ref ID J:61052] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Prnp-MAPT)43Vle, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Ishihara T; Hong M; Zhang B; Nakagawa Y; Lee MK; Trojanowski JQ; Lee VM. 1999. Age-dependent emergence and progression of a tauopathy in transgenic mice overexpressing the shortest human tau isoform. Neuron 24(3):751-62. [PubMed: 10595524] [MGI Ref ID J:61052]
Additional References
Nakashima H; Ishihara T; Yokota O; Terada S; Trojanowski JQ; Lee VM; Kuroda S. 2004. Effects of alpha-tocopherol on an animal model of tauopathies. Free Radic Biol Med 37(2):176-86. [PubMed: 15203189] [MGI Ref ID J:90946]
Tg(Prnp-MAPT)43Vle relatedLim J; Balastik M; Lee TH; Nakamura K; Liou YC; Sun A; Finn G; Pastorino L; Lee VM; Lu KP. 2008. Pin1 has opposite effects on wild-type and P301L tau stability and tauopathy. J Clin Invest 118(5):1877-89. [PubMed: 18431510] [MGI Ref ID J:135144]
Nakamura K; Greenwood A; Binder L; Bigio EH; Denial S; Nicholson L; Zhou XZ; Lu KP. 2012. Proline isomer-specific antibodies reveal the early pathogenic tau conformation in Alzheimer's disease. Cell 149(1):232-44. [PubMed: 22464332] [MGI Ref ID J:186082]
Nakashima H; Ishihara T; Yokota O; Terada S; Trojanowski JQ; Lee VM; Kuroda S. 2004. Effects of alpha-tocopherol on an animal model of tauopathies. Free Radic Biol Med 37(2):176-86. [PubMed: 15203189] [MGI Ref ID J:90946]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as as hemizygotes. Homozygous mice die at about 3 months of age. It is uncertain if homozygous males can reproduce before death. Coat color expected from breeding: Albino, Agouti, Black. Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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