Go to JAX^® Mice Query Form

General Terms and Conditions

Former Name	C57BL/10ScCr    (Changed: 15-DEC-04 )
	C57BL/10ScNCr    (Changed: 15-DEC-04 )
Genes & Alleles	Tlr4;   Tlr4lps-del;

---

Product Information

Strain Details

Type Inbred Strain Additional information on Inbred Strains. Type JAX® GEMM® Strain - Spontaneous Mutation Additional information on JAX® GEMM® Strains. Mating System Sibling x Sibling         (Female x Male) Species laboratory mouse H2 Haplotype b Generation F131+18 (18-OCT-07)

Appearance
black
Related Genotype: a/a

Important Note
This strain is homozygous for the defective lipopolysaccharide response, deletion allele Tlr4^lps-del.

Strain Description
The breeder pair of C57BL/10ScN mice, obtained from NIH, that were the progenitors of all mice of this strain at The Jackson Laboratory were tested by PCR analysis for the spontaneous Il12rb2 mutation described by Poltorak et al. (J. Immunol. 167:2106-2111, 2001) and found to carry only the wild type Il12rb2 allele. C57BL/10ScN mice have a deletion of the Tlr4 gene that results in absence of both mRNA and protein and thus in defective response to LPS stimulation. Tlr4^lps-del differs from the Tlr4^Lps-d mutation of C3H/HeJ mice, a point mutation that causes an amino acid substitution. The allele for normal LPS response, Tlr4^lps-n, occurs in most other C3H and C57BL/10 substrains and most other mouse strains.

Strain Development
The C57BL/6 and C57BL/10 sublines of the C57BL mouse line diverged sometime prior to 1937. The C57BL/10 subline was passed from Clarence Little to J.P. Scott (lab code Sc) at F26 and from Scott to George Snell (Sn) at F35 around 1947. In 1953, Snell sent mice of this line, C57BL/10ScSn (also known as C57BL/10Sn) to the National Institutes of Health (N) (Vogel et al. 1979), where it was initially designated C57BL/10ScSnN; however, Snell's lab code was dropped early on, and the strain has been called C57BL/10ScN for most of its history at NIH (Hansen et al. 2000). Clarence Reeder (Cr) began maintaining mice of this line at the Frederick Cancer Research Center, Frederick, MD in 1961 (Vogel et al. 1979), and mice distributed from this colony were designated C57BL/10ScNCr (Hansen et al. 2000). The breeding stock at the Cancer Research Center is replenished regularly with C57BL/10ScN from NIHAGR (Hansen et al. 2000), and mice of both colonies are screened annually for 14 biochemical markers and every two years by skin grafting; therefore, C57BL/10ScN, C57BL/10ScNCr and, presumably, C57BL/10ScCr are names for the same subline of C57BL/10.

A pedigreed sibling pair of C57BL/10ScN mice obtained in August, 2000 at N130 from the NIH foundation colony was used to found the colony now maintained at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol		Tlr4lps-del
Allele Name		defective lipopolysaccharide response, deletion
Common Name(s)		Tlr4-;
Strain of Origin		C57BL/10ScN
Gene Symbol and Name		Tlr4, toll-like receptor 4
Chromosome		4
Gene Common Name(s)		ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TOLL; hToll; lipopolysaccharide response;
General Note		C57BL/10ScN mice carry this allele.
Molecular Note		This allele has been characterized by lack of mRNA owing to a deletion of the locus. 74723bp of genomic DNA sequence have been removed, apparently encompasing only the Tlr4 gene. [J:122203] [J:51522]

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Related Strains

C57BL Strains

000665	C57BL/10J
000476	C57BL/10ScSnJ
000666	C57BL/10SnJ
001822	C57BL/10SxJ
001197	C57BL/10WtRkJ
000663	C57BL/6By
001139	C57BL/6ByJ
000664	C57BL/6J
000924	C57BL/6JEiJ
005304	C57BL/6NJ

View C57BL Strains     (10 strains)

Strains carrying   Tlr4^lps-del allele

007227

B6.B10ScN-Tlr4lps-del/JthJ

View Strains carrying   Tlr4^lps-del     (1 strain)

Strains carrying other alleles of Tlr4

002930	C.C3-Tlr4Lps-d/J
005973	C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326	C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968	C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
000659	C3H/HeJ
005972	C3H/HeJBirLtJ

View Strains carrying other alleles of Tlr4     (6 strains)

Additional Web Information

JAX Notes, Summer 2005; 498. Toll-like Receptor JAX® Mice for Immunological Research.

Animal Health Reports

Room Number           FGB27

Research Applications

This mouse can be used to support research in many areas including:

Tlr4^lps-del related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers (Tlr deficiency)
Immunodeficiency (Tlr deficiency)
Inflammation (Tlr deficiency)

References

Additional References

---

Price and Supply Information

Strain Name:	C57BL/10ScNJ
Stock Number:	003752

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.

• USA, Canada and Mexico
• International

*NO Shipping Destination selected!

 

Supply Details

Standard Supply	Repository-Live. No age specifications accepted. Colony sized to produce minimal quantities (typically up to 6 mice) upon order receipt; one order per strain. Expected delivery: 1-3 months. Larger quantities or custom orders arranged upon request.
Supply Notes	Usually shipped between four and six weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Go to JAX^® Mice Query Form