Description

Strain Information

Former Names C57BL/10ScCr    (Changed: 15-DEC-04 ) C57BL/10ScNCr    (Changed: 15-DEC-04 ) Type Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Type Inbred Strain; Additional information on Inbred Strains. Visit our online Nomenclature tutorial. Mating System Sibling x Sibling         (Female x Male)   01-MAR-06 Species laboratory mouse H2 Haplotype b Generation F131+21 (31-DEC-08)

Appearance
black
Related Genotype: a/a

Important Note
This strain is homozygous for the defective lipopolysaccharide response, deletion allele Tlr4^lps-del.

Description
The breeder pair of C57BL/10ScN mice, obtained from NIH, that were the progenitors of all mice of this strain at The Jackson Laboratory were tested by PCR analysis for the spontaneous Il12rb2 mutation described by Poltorak et al. (J. Immunol. 167:2106-2111, 2001) and found to carry only the wild type Il12rb2 allele. C57BL/10ScN mice have a deletion of the Tlr4 gene that results in absence of both mRNA and protein and thus in defective response to LPS stimulation. Tlr4^lps-del differs from the Tlr4^Lps-d mutation of C3H/HeJ mice, a point mutation that causes an amino acid substitution. The allele for normal LPS response, Tlr4^lps-n, occurs in most other C3H and C57BL/10 substrains and most other mouse strains.

Development
The C57BL/6 and C57BL/10 sublines of the C57BL mouse line diverged sometime prior to 1937. The C57BL/10 subline was passed from Clarence Little to J.P. Scott (lab code Sc) at F26 and from Scott to George Snell (Sn) at F35 around 1947. In 1953, Snell sent mice of this line, C57BL/10ScSn (also known as C57BL/10Sn) to the National Institutes of Health (N) (Vogel et al. 1979), where it was initially designated C57BL/10ScSnN; however, Snell's lab code was dropped early on, and the strain has been called C57BL/10ScN for most of its history at NIH (Hansen et al. 2000). Clarence Reeder (Cr) began maintaining mice of this line at the Frederick Cancer Research Center, Frederick, MD in 1961 (Vogel et al. 1979), and mice distributed from this colony were designated C57BL/10ScNCr (Hansen et al. 2000). The breeding stock at the Cancer Research Center is replenished regularly with C57BL/10ScN from NIHAGR (Hansen et al. 2000), and mice of both colonies are screened annually for 14 biochemical markers and every two years by skin grafting; therefore, C57BL/10ScN, C57BL/10ScNCr and, presumably, C57BL/10ScCr are names for the same subline of C57BL/10.

A pedigreed sibling pair of C57BL/10ScN mice obtained in August, 2000 at N130 from the NIH foundation colony was used to found the colony now maintained at The Jackson Laboratory.

Related Strains

C57BL Strains

000665	C57BL/10J
000476	C57BL/10ScSnJ
000666	C57BL/10SnJ
001822	C57BL/10SxJ
001197	C57BL/10WtRkJ
000663	C57BL/6By
001139	C57BL/6ByJ
009123	C57BL/6HaJ
000664	C57BL/6J
000924	C57BL/6JEiJ
005304	C57BL/6NJ

View C57BL Strains     (11 strains)

Strains carrying   Tlr4^lps-del allele

007227

B6.B10ScN-Tlr4lps-del/JthJ

View Strains carrying   Tlr4^lps-del     (1 strain)

Strains carrying other alleles of Tlr4

000029	BXD29-Tlr4lps-2J/J
002930	C.C3-Tlr4Lps-d/J
005973	C3Bir.129P2(B6)-Il10C3Bir/LtJ
004326	C3Bir.129P2(B6)-Il10tm1Cgn/Lt
003968	C3Bir.129P2(B6)-Il10tm1Cgn/LtJ
000659	C3H/HeJ
005972	C3H/HeJBirLtJ

View Strains carrying other alleles of Tlr4     (7 strains)

Additional Web Information

JAX^® NOTES, Summer 2005; 498. Toll-like Receptor JAX^® Mice for Immunological Research.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tlr4^lps-del/Tlr4^lps-del

        involves: C57BL/10ScN

• immune system phenotype
• decreased interleukin-10 secretion (MGI Ref ID J:147023)
  • production is suppressed in response to E coli with or without adenosine
• decreased interleukin-6 secretion (MGI Ref ID J:147023)
  • production is suppressed in response to E coli

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tlr4^lps-del related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
      Tlr deficiency
Immunodeficiency
      Tlr deficiency
Inflammation
      Tlr deficiency

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tlr4lps-del
Allele Name		defective lipopolysaccharide response, deletion
Allele Type		Spontaneous
Common Name(s)		Tlr4-;
Strain of Origin		C57BL/10ScN
Gene Symbol and Name		Tlr4, toll-like receptor 4
Chromosome		4
Gene Common Name(s)		ARMD10; CD284; Lps; RAS-like, family 2, locus 8; Rasl2-8; TOLL; hToll; lipopolysaccharide response;
General Note		C57BL/10ScN, C57BL/10ScNJ, and C57BL10/ScCr mice carry this allele.
Molecular Note		This allele has been characterized by lack of mRNA owing to a deletion of the locus. 74723bp of genomic DNA sequence have been removed, apparently encompasing only the Tlr4 gene. [MGI Ref ID J:122203] [MGI Ref ID J:51522]

Genotyping

Genotyping Information

Genotyping Protocols

Tlr4^lps-del, Melt Curve Analysis
Tlr4^lps-del, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Additional References

Tlr4^lps-del related

Agace W; Hedges S; Svanborg C. 1992. Lps genotype in the C57 black mouse background and its influence on the interleukin-6 response to E. coli urinary tract infection. Scand J Immunol 35(5):531-8. [PubMed: 1579857]  [MGI Ref ID J:3436]

Amiel E; Alonso A; Uematsu S; Akira S; Poynter ME; Berwin B. 2009. Pivotal Advance: Toll-like receptor regulation of scavenger receptor-A-mediated phagocytosis. J Leukoc Biol 85(4):595-605. [PubMed: 19112093]  [MGI Ref ID J:146918]

Beisswenger C; Lysenko ES; Weiser JN. 2009. Early bacterial colonization induces toll-like receptor-dependent transforming growth factor beta signaling in the epithelium. Infect Immun 77(5):2212-20. [PubMed: 19255194]  [MGI Ref ID J:148184]

Bhattacharyya S; Dudeja PK; Tobacman JK. 2008. Lipopolysaccharide activates NF-kappaB by TLR4-Bcl10-dependent and independent pathways in colonic epithelial cells. Am J Physiol Gastrointest Liver Physiol 295(4):G784-90. [PubMed: 18718996]  [MGI Ref ID J:142282]

Binck BW; Tsen MF; Islas M; White DJ; Schultz RA; Willis MS; Garcia JV; Horton JW; Thomas JA. 2005. Bone marrow-derived cells contribute to contractile dysfunction in endotoxic shock. Am J Physiol Heart Circ Physiol 288(2):H577-83. [PubMed: 15458952]  [MGI Ref ID J:96165]

Biragyn A; Coscia M; Nagashima K; Sanford M; Young HA; Olkhanud P. 2008. Murine beta-defensin 2 promotes TLR-4/MyD88-mediated and NF-kappaB-dependent atypical death of APCs via activation of TNFR2. J Leukoc Biol 83(4):998-1008. [PubMed: 18192488]  [MGI Ref ID J:134193]

Burch LH; Yang IV; Whitehead GS; Chao FG; Berman KG; Schwartz DA. 2006. The transcriptional response to lipopolysaccharide reveals a role for interferon-gamma in lung neutrophil recruitment. Am J Physiol Lung Cell Mol Physiol 291(4):L677-82. [PubMed: 16766576]  [MGI Ref ID J:144404]

Burns E; Bachrach G; Shapira L; Nussbaum G. 2006. Cutting Edge: TLR2 is required for the innate response to Porphyromonas gingivalis: activation leads to bacterial persistence and TLR2 deficiency attenuates induced alveolar bone resorption. J Immunol 177(12):8296-300. [PubMed: 17142724]  [MGI Ref ID J:140676]

Caso JR; Pradillo JM; Hurtado O; Lorenzo P; Moro MA; Lizasoain I. 2007. Toll-like receptor 4 is involved in brain damage and inflammation after experimental stroke. Circulation 115(12):1599-608. [PubMed: 17372179]  [MGI Ref ID J:133065]

Chan YR; Liu JS; Pociask DA; Zheng M; Mietzner TA; Berger T; Mak TW; Clifton MC; Strong RK; Ray P; Kolls JK. 2009. Lipocalin 2 is required for pulmonary host defense against Klebsiella infection. J Immunol 182(8):4947-56. [PubMed: 19342674]  [MGI Ref ID J:147498]

Cohen-Sfady M; Pevsner-Fischer M; Margalit R; Cohen IR. 2009. Heat shock protein 60, via MyD88 innate signaling, protects B cells from apoptosis, spontaneous and induced. J Immunol 183(2):890-6. [PubMed: 19561102]  [MGI Ref ID J:151657]

Costalonga M; Zell T. 2007. Lipopolysaccharide enhances in vivo interleukin-2 production and proliferation by naive antigen-specific CD4 T cells via a Toll-like receptor 4-dependent mechanism. Immunology 122(1):124-30. [PubMed: 17484770]  [MGI Ref ID J:125617]

Csoka B; Nemeth ZH; Virag L; Gergely P; Leibovich SJ; Pacher P; Sun CX; Blackburn MR; Vizi ES; Deitch EA; Hasko G. 2007. A2A adenosine receptors and C/EBPbeta are crucially required for IL-10 production by macrophages exposed to Escherichia coli. Blood 110(7):2685-95. [PubMed: 17525287]  [MGI Ref ID J:147023]

D'Avila H; Almeida PE; Roque NR; Castro-Faria-Neto HC; Bozza PT. 2007. Toll-Like Receptor-2-Mediated C-C Chemokine Receptor 3 and Eotaxin-Driven Eosinophil Influx Induced by Mycobacterium bovis BCG Pleurisy. Infect Immun 75(3):1507-11. [PubMed: 17158890]  [MGI Ref ID J:118500]

D'Avila H; Melo RC; Parreira GG; Werneck-Barroso E; Castro-Faria-Neto HC; Bozza PT. 2006. Mycobacterium bovis bacillus Calmette-Guerin induces TLR2-mediated formation of lipid bodies: intracellular domains for eicosanoid synthesis in vivo. J Immunol 176(5):3087-97. [PubMed: 16493068]  [MGI Ref ID J:129415]

Darville T; O'Neill JM; Andrews CW Jr; Nagarajan UM; Stahl L; Ojcius DM. 2003. Toll-like receptor-2, but not Toll-like receptor-4, is essential for development of oviduct pathology in chlamydial genital tract infection. J Immunol 171(11):6187-97. [PubMed: 14634135]  [MGI Ref ID J:106730]

David MD; Cochrane CL; Duncan SK; Schrader JW. 2005. Pure lipopolysaccharide or synthetic lipid A induces activation of p21Ras in primary macrophages through a pathway dependent on Src family kinases and PI3K. J Immunol 175(12):8236-41. [PubMed: 16339563]  [MGI Ref ID J:122258]

Desai MS; Mariscalco MM; Tawil A; Vallejo JG; Smith CW. 2008. Atherogenic diet-induced hepatitis is partially dependent on murine TLR4. J Leukoc Biol 83(6):1336-44. [PubMed: 18334542]  [MGI Ref ID J:136848]

Ehl S; Bischoff R; Ostler T; Vallbracht S; Schulte-Monting J; Poltorak A; Freudenberg M. 2004. The role of Toll-like receptor 4 versus interleukin-12 in immunity to respiratory syncytial virus. Eur J Immunol 34(4):1146-53. [PubMed: 15048726]  [MGI Ref ID J:88878]

Espinassous Q; Garcia-de-Paco E; Garcia-Verdugo I; Synguelakis M; von Aulock S; Sallenave JM; McKenzie AN; Kanellopoulos J. 2009. IL-33 enhances lipopolysaccharide-induced inflammatory cytokine production from mouse macrophages by regulating lipopolysaccharide receptor complex. J Immunol 183(2):1446-55. [PubMed: 19553541]  [MGI Ref ID J:151399]

Ganta RR; Wilkerson MJ; Cheng C; Rokey AM; Chapes SK. 2002. Persistent Ehrlichia chaffeensis infection occurs in the absence of functional major histocompatibility complex class II genes. Infect Immun 70(1):380-8. [PubMed: 11748204]  [MGI Ref ID J:74569]

Ha T; Li Y; Hua F; Ma J; Gao X; Kelley J; Zhao A; Haddad GE; Williams DL; William Browder I; Kao RL; Li C. 2005. Reduced cardiac hypertrophy in toll-like receptor 4-deficient mice following pressure overload. Cardiovasc Res 68(2):224-34. [PubMed: 15967420]  [MGI Ref ID J:106358]

Haziot A; Hijiya N; Gangloff SC; Silver J; Goyert SM. 2001. Induction of a novel mechanism of accelerated bacterial clearance by lipopolysaccharide in CD14-deficient and Toll-like receptor 4-deficient mice. J Immunol 166(2):1075-8. [PubMed: 11145687]  [MGI Ref ID J:127072]

He RL; Zhou J; Hanson CZ; Chen J; Cheng N; Ye RD. 2009. Serum amyloid A induces G-CSF expression and neutrophilia via Toll-like receptor 2. Blood 113(2):429-37. [PubMed: 18952897]  [MGI Ref ID J:144859]

Heimesaat MM; Fischer A; Jahn HK; Niebergall J; Freudenberg M; Blaut M; Liesenfeld O; Schumann RR; Gobel UB; Bereswill S. 2007. Exacerbation of murine ileitis by Toll-like receptor 4 mediated sensing of lipopolysaccharide from commensal Escherichia coli. Gut 56(7):941-8. [PubMed: 17255219]  [MGI Ref ID J:131231]

Heimesaat MM; Fischer A; Siegmund B; Kupz A; Niebergall J; Fuchs D; Jahn HK; Freudenberg M; Loddenkemper C; Batra A; Lehr HA; Liesenfeld O; Blaut M; Gobel UB; Schumann RR; Bereswill S. 2007. Shift towards pro-inflammatory intestinal bacteria aggravates acute murine colitis via Toll-like receptors 2 and 4. PLoS ONE 2(7):e662. [PubMed: 17653282]  [MGI Ref ID J:129318]

Hua F; Ha T; Ma J; Li Y; Kelley J; Gao X; Browder IW; Kao RL; Williams DL; Li C. 2007. Protection against myocardial ischemia/reperfusion injury in TLR4-deficient mice is mediated through a phosphoinositide 3-kinase-dependent mechanism. J Immunol 178(11):7317-24. [PubMed: 17513782]  [MGI Ref ID J:147828]

Hua F; Ma J; Ha T; Kelley JL; Kao RL; Schweitzer JB; Kalbfleisch JH; Williams DL; Li C. 2009. Differential roles of TLR2 and TLR4 in acute focal cerebral ischemia/reperfusion injury in mice. Brain Res 1262:100-8. [PubMed: 19401158]  [MGI Ref ID J:148091]

Hua F; Ma J; Ha T; Xia Y; Kelley J; Williams DL; Kao RL; Browder IW; Schweitzer JB; Kalbfleisch JH; Li C. 2007. Activation of Toll-like receptor 4 signaling contributes to hippocampal neuronal death following global cerebral ischemia/reperfusion. J Neuroimmunol 190(1-2):101-11. [PubMed: 17884182]  [MGI Ref ID J:129001]

Ince MN; Elliott DE; Setiawan T; Blum A; Metwali A; Wang Y; Urban JF Jr; Weinstock JV. 2006. Heligmosomoides polygyrus induces TLR4 on murine mucosal T cells that produce TGFbeta after lipopolysaccharide stimulation. J Immunol 176(2):726-9. [PubMed: 16393954]  [MGI Ref ID J:126606]

Jawdat DM; Rowden G; Marshall JS. 2006. Mast cells have a pivotal role in TNF-independent lymph node hypertrophy and the mobilization of Langerhans cells in response to bacterial peptidoglycan. J Immunol 177(3):1755-62. [PubMed: 16849485]  [MGI Ref ID J:137976]

Jayachandran M; Brunn GJ; Karnicki K; Miller RS; Owen WG; Miller VM. 2007. In vivo effects of lipopolysaccharide and TLR4 on platelet production and activity: implications for thrombotic risk. J Appl Physiol 102(1):429-33. [PubMed: 16916914]  [MGI Ref ID J:135961]

John B; Crispe IN. 2005. TLR-4 regulates CD8+ T cell trapping in the liver. J Immunol 175(3):1643-50. [PubMed: 16034104]  [MGI Ref ID J:107284]

Joosten LA; Koenders MI; Smeets RL; Heuvelmans-Jacobs M; Helsen MM; Takeda K; Akira S; Lubberts E; van de Loo FA; van den Berg WB. 2003. Toll-like receptor 2 pathway drives streptococcal cell wall-induced joint inflammation: critical role of myeloid differentiation factor 88. J Immunol 171(11):6145-53. [PubMed: 14634130]  [MGI Ref ID J:119063]

Kalis C; Kanzler B; Lembo A; Poltorak A; Galanos C; Freudenberg MA. 2003. Toll-like receptor 4 expression levels determine the degree of LPS-susceptibility in mice. Eur J Immunol 33(3):798-805. [PubMed: 12616500]  [MGI Ref ID J:82413]

Kane CM; Jung E; Pearce EJ. 2008. Schistosoma mansoni egg antigen-mediated modulation of Toll-like receptor (TLR)-induced activation occurs independently of TLR2, TLR4, and MyD88. Infect Immun 76(12):5754-9. [PubMed: 18824534]  [MGI Ref ID J:143320]

Kelly MM; McNagny K; Williams DL; van Rooijen N; Maxwell L; Gwozd C; Mody CH; Kubes P. 2008. The lung responds to zymosan in a unique manner independent of toll-like receptors, complement, and dectin-1. Am J Respir Cell Mol Biol 38(2):227-38. [PubMed: 17717323]  [MGI Ref ID J:146394]

Khan MA; Ma C; Knodler LA; Valdez Y; Rosenberger CM; Deng W; Finlay BB; Vallance BA. 2006. Toll-like receptor 4 contributes to colitis development but not to host defense during Citrobacter rodentium infection in mice. Infect Immun 74(5):2522-36. [PubMed: 16622187]  [MGI Ref ID J:108095]

Kropf P; Freudenberg MA; Modolell M; Price HP; Herath S; Antoniazi S; Galanos C; Smith DF; Muller I. 2004. Toll-like receptor 4 contributes to efficient control of infection with the protozoan parasite Leishmania major. Infect Immun 72(4):1920-8. [PubMed: 15039311]  [MGI Ref ID J:88996]

Kropf P; Freudenberg N; Kalis C; Modolell M; Herath S; Galanos C; Freudenberg M; Muller I. 2004. Infection of C57BL/10ScCr and C57BL/10ScNCr mice with Leishmania major reveals a role for Toll-like receptor 4 in the control of parasite replication. J Leukoc Biol 76(1):48-57. [PubMed: 15039466]  [MGI Ref ID J:91014]

Lebeis SL; Bommarius B; Parkos CA; Sherman MA; Kalman D. 2007. TLR signaling mediated by MyD88 is required for a protective innate immune response by neutrophils to Citrobacter rodentium. J Immunol 179(1):566-77. [PubMed: 17579078]  [MGI Ref ID J:149408]

Lee JS; Frevert CW; Matute-Bello G; Wurfel MM; Wong VA; Lin SM; Ruzinski J; Mongovin S; Goodman RB; Martin TR. 2005. TLR-4 pathway mediates the inflammatory response but not bacterial elimination in E. coli pneumonia. Am J Physiol Lung Cell Mol Physiol 289(5):L731-8. [PubMed: 16024722]  [MGI Ref ID J:115440]

Lorne E; Zmijewski JW; Zhao X; Liu G; Tsuruta Y; Park YJ; Dupont H; Abraham E. 2008. Role of extracellular superoxide in neutrophil activation: interactions between xanthine oxidase and TLR4 induce proinflammatory cytokine production. Am J Physiol Cell Physiol 294(4):C985-93. [PubMed: 18287332]  [MGI Ref ID J:136585]

Mandell L; Moran AP; Cocchiarella A; Houghton J; Taylor N; Fox JG; Wang TC; Kurt-Jones EA. 2004. Intact gram-negative Helicobacter pylori, Helicobacter felis, and Helicobacter hepaticus bacteria activate innate immunity via toll-like receptor 2 but not toll-like receptor 4. Infect Immun 72(11):6446-54. [PubMed: 15501775]  [MGI Ref ID J:93282]

Mann PB; Elder KD; Kennett MJ; Harvill ET. 2004. Toll-like receptor 4-dependent early elicited tumor necrosis factor alpha expression is critical for innate host defense against Bordetella bronchiseptica. Infect Immun 72(11):6650-8. [PubMed: 15501798]  [MGI Ref ID J:93263]

Mann PB; Kennett MJ; Harvill ET. 2004. Toll-like receptor 4 is critical to innate host defense in a murine model of bordetellosis. J Infect Dis 189(5):833-6. [PubMed: 14976600]  [MGI Ref ID J:88587]

Mann PB; Wolfe D; Latz E; Golenbock D; Preston A; Harvill ET. 2005. Comparative toll-like receptor 4-mediated innate host defense to Bordetella infection. Infect Immun 73(12):8144-52. [PubMed: 16299309]  [MGI Ref ID J:104298]

Martin SF; Dudda JC; Bachtanian E; Lembo A; Liller S; Durr C; Heimesaat MM; Bereswill S; Fejer G; Vassileva R; Jakob T; Freudenberg N; Termeer CC; Johner C; Galanos C; Freudenberg MA. 2008. Toll-like receptor and IL-12 signaling control susceptibility to contact hypersensitivity. J Exp Med 205(9):2151-62. [PubMed: 18725520]  [MGI Ref ID J:138813]

Merlin T; Sing A; Nielsen PJ; Galanos C; Freudenberg MA. 2001. Inherited IL-12 unresponsiveness contributes to the high LPS resistance of the lps(d) C57BL/10ScCr mouse J Immunol 166(1):566-73. [PubMed: 11123338]  [MGI Ref ID J:66394]

Minguet S; Dopfer EP; Pollmer C; Freudenberg MA; Galanos C; Reth M; Huber M; Schamel WW. 2008. Enhanced B-cell activation mediated by TLR4 and BCR crosstalk. Eur J Immunol 38(9):2475-87. [PubMed: 18819072]  [MGI Ref ID J:140162]

Mullaly SC; Kubes P. 2007. Mast cell-expressed complement receptor, not TLR2, is the main detector of zymosan in peritonitis. Eur J Immunol 37(1):224-34. [PubMed: 17154261]  [MGI Ref ID J:117071]

Mullaly SC; Kubes P. 2006. The role of TLR2 in vivo following challenge with Staphylococcus aureus and prototypic ligands. J Immunol 177(11):8154-63. [PubMed: 17114491]  [MGI Ref ID J:140680]

Muller I; Freudenberg M; Kropf P; Kiderlen AF; Galanos C. 1997. Leishmania major infection in C57BL/10 mice differing at the Lps locus: a new non-healing phenotype. Med Microbiol Immunol (Berl) 186(2-3):75-81. [PubMed: 9403834]  [MGI Ref ID J:44519]

Nagarajan UM; Ojcius DM; Stahl L; Rank RG; Darville T. 2005. Chlamydia trachomatis induces expression of IFN-gamma-inducible protein 10 and IFN-beta independent of TLR2 and TLR4, but largely dependent on MyD88. J Immunol 175(1):450-60. [PubMed: 15972679]  [MGI Ref ID J:100592]

Necela BM; Su W; Thompson EA. 2008. Toll-like receptor 4 mediates cross-talk between peroxisome proliferator-activated receptor gamma and nuclear factor-kappaB in macrophages. Immunology 125(3):344-58. [PubMed: 18422969]  [MGI Ref ID J:144451]

Netea MG; Kullberg BJ; Jacobs LE; Verver-Jansen TJ; van der Ven-Jongekrijg J; Galama JM; Stalenhoef AF; Dinarello CA; Van der Meer JW. 2004. Chlamydia pneumoniae stimulates IFN-gamma synthesis through MyD88-dependent, TLR2- and TLR4-independent induction of IL-18 release. J Immunol 173(2):1477-82. [PubMed: 15240744]  [MGI Ref ID J:91941]

Nussbaum G; Ben-Adi S; Genzler T; Sela M; Rosen G. 2009. Involvement of Toll-like receptors 2 and 4 in the innate immune response to Treponema denticola and its outer sheath components. Infect Immun 77(9):3939-47. [PubMed: 19596768]  [MGI Ref ID J:152226]

Oyama J; Blais C Jr; Liu X; Pu M; Kobzik L; Kelly RA; Bourcier T. 2004. Reduced myocardial ischemia-reperfusion injury in toll-like receptor 4-deficient mice. Circulation 109(6):784-9. [PubMed: 14970116]  [MGI Ref ID J:102150]

Palliser D; Huang Q; Hacohen N; Lamontagne SP; Guillen E; Young RA; Eisen HN. 2004. A role for Toll-like receptor 4 in dendritic cell activation and cytolytic CD8+ T cell differentiation in response to a recombinant heat shock fusion protein. J Immunol 172(5):2885-93. [PubMed: 14978090]  [MGI Ref ID J:88229]

Pfannes SD; Muller B; Korner S; Bessler WG; Hoffmann P. 2001. Induction of soluble antitumoral mediators by synthetic analogues of bacterial lipoprotein in bone marrow-derived macrophages from LPS-responder and -nonresponder mice. J Leukoc Biol 69(4):590-7. [PubMed: 11310845]  [MGI Ref ID J:68625]

Poltorak A; He X; Smirnova I; Liu MY; Huffel CV; Du X; Birdwell D; Alejos E; Silva M; Galanos C; Freudenberg M; Ricciardi-Castagnoli P; Layton B; Beutler B. 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 282(5396):2085-8. [PubMed: 9851930]  [MGI Ref ID J:51522]

Poltorak A; Merlin T; Nielsen PJ; Sandra O; Smirnova I; Schupp I; Boehm T; Galanos C; Freudenberg MA. 2001. A point mutation in the il-12rbeta2 gene underlies the il-12 unresponsiveness of lps-defective c57bl/10sccr mice. J Immunol 167(4):2106-11. [PubMed: 11489994]  [MGI Ref ID J:70821]

Poltorak A; Smirnova I; Clisch R; Beutler B. 2000. Limits of a deletion spanning Tlr4 in C57BL/10ScCr mice. J Endotoxin Res 6(1):51-6. [PubMed: 11061032]  [MGI Ref ID J:122203]

Power MR; Marshall JS; Yamamoto M; Akira S; Lin TJ. 2006. The myeloid differentiation factor 88 is dispensable for the development of a delayed host response to Pseudomonas aeruginosa lung infection in mice. Clin Exp Immunol 146(2):323-9. [PubMed: 17034585]  [MGI Ref ID J:113568]

Quintana FJ; Solomon A; Cohen IR; Nussbaum G. 2008. Induction of IgG3 to LPS via Toll-like receptor 4 co-stimulation. PLoS ONE 3(10):e3509. [PubMed: 18946502]  [MGI Ref ID J:144630]

Qureshi ST; Lariviere L; Leveque G; Clermont S; Moore KJ; Gros P ; Malo D. 1999. Endotoxin-tolerant mice have mutations in Toll-like receptor 4 (Tlr4) [see comments] J Exp Med 189(4):615-25. [PubMed: 9989976]  [MGI Ref ID J:53343]

Riad A; Jager S; Sobirey M; Escher F; Yaulema-Riss A; Westermann D; Karatas A; Heimesaat MM; Bereswill S; Dragun D; Pauschinger M; Schultheiss HP; Tschope C. 2008. Toll-like receptor-4 modulates survival by induction of left ventricular remodeling after myocardial infarction in mice. J Immunol 180(10):6954-61. [PubMed: 18453617]  [MGI Ref ID J:134865]

Rolls A; Shechter R; London A; Ziv Y; Ronen A; Levy R; Schwartz M. 2007. Toll-like receptors modulate adult hippocampal neurogenesis. Nat Cell Biol 9(9):1081-8. [PubMed: 17704767]  [MGI Ref ID J:129957]

Rumbaut RE; Bellera RV; Randhawa JK; Shrimpton CN; Dasgupta SK; Dong JF; Burns AR. 2006. Endotoxin enhances microvascular thrombosis in mouse cremaster venules via a TLR4-dependent, neutrophil-independent mechanism. Am J Physiol Heart Circ Physiol 290(4):H1671-9. [PubMed: 16284241]  [MGI Ref ID J:108441]

Schulthess FT ; Paroni F ; Sauter NS ; Shu L ; Ribaux P ; Haataja L ; Strieter RM ; Oberholzer J ; King CC ; Maedler K. 2009. CXCL10 impairs beta cell function and viability in diabetes through TLR4 signaling. Cell Metab 9(2):125-39. [PubMed: 19187771]  [MGI Ref ID J:146651]

Scott MJ; Billiar TR. 2008. Beta2-integrin-induced p38 MAPK activation is a key mediator in the CD14/TLR4/MD2-dependent uptake of lipopolysaccharide by hepatocytes. J Biol Chem 283(43):29433-46. [PubMed: 18701460]  [MGI Ref ID J:142561]

Seimon TA; Obstfeld A; Moore KJ; Golenbock DT; Tabas I. 2006. Combinatorial pattern recognition receptor signaling alters the balance of life and death in macrophages. Proc Natl Acad Sci U S A 103(52):19794-9. [PubMed: 17167049]  [MGI Ref ID J:118240]

Shao B; Lu M; Katz SC; Varley AW; Hardwick J; Rogers TE; Ojogun N; Rockey DC; Dematteo RP; Munford RS. 2007. A host lipase detoxifies bacterial lipopolysaccharides in the liver and spleen. J Biol Chem 282(18):13726-35. [PubMed: 17322564]  [MGI Ref ID J:121874]

Sing A; Roggenkamp A; Geiger AM; Heesemann J. 2002. Yersinia enterocolitica evasion of the host innate immune response by V antigen-induced IL-10 production of macrophages is abrogated in IL-10-deficient mice. J Immunol 168(3):1315-21. [PubMed: 11801671]  [MGI Ref ID J:127288]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB27

Colony Maintenance

Mating System	Sibling x Sibling         (Female x Male)   01-MAR-06
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes	Usually shipped between four and six weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Important Note
This strain is homozygous for the defective lipopolysaccharide response, deletion allele Tlr4lps-del.

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
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JAX^® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.