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Strain Name:

FVB/N-Tg(Eno2CDK5R1)1Jdm/J

Stock Number:

003753

Availability:

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Genes & Alleles   CDK5R1;   Eno2;   Tg(Eno2CDK5R1)1Jdm;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Michael Ahlijanian,   Pfizer Central Research

Strain Description
These transgenic animals display a robust expression of the amino terminal proteolytic fragment p25 of human CDK5R1. Expression is detected in the amygdala, thalamus/hypothalamus, cerebral cortex, and cerebellum. The p25 fragment acts as a positive allosteric regulator of cyclin-dependent kinase 5 (Cdk5), a kinase known to phosphorylate the microtubule-binding protein tau. A two-fold increase in the catalytic activity of Cdk5 is observed in these mice. In contrast to wildtype control mice, brains from 4-month-old exhibit the presence of tau and neurofilament phosphoepitopes. Utlrastructurally, axonal swelling in neurons from the amygdala and spinal cord is observed. Affected neurons possess numerous, abnormally clustered mitochondria and lysosomes, with some evidence of cytoskeletal disorganization. Mice exhibit whole-body tremors at 4-9 weeks of age and enhanced open-field locomotor activity. Aspects of this phenotype are similar to Alzheimer's, Pick's and other neurodegenerative diseases.

Mammalian Phenotype Terms assigned by genotype

Tg(Eno2CDK5R1)1Jdm/?

        involves: FVB/N
  • behavior/neurological phenotype
  • abnormal locomotor activity (MGI Ref ID J:82825)
    • starting at 5-7 weeks of age, mice show increased open-field locomotor activity, which is 2-fold greater than wild-type at 16 weeks
  • decreased anxiety-related response (MGI Ref ID J:82825)
    • mice show increased open-field locomotor activity, which is 2-fold greater than wild-type at 16 weeks and increased locomotor activity in activity chambers
    • in elevated-plus maze, mice aged 9-12 weeks spend more time on the open arms compared to wild-type animals
  • tremors (MGI Ref ID J:82825)
    • 4-9 week-old mice display whole-body exertion tremors
  • nervous system phenotype
  • abnormal axon morphology (MGI Ref ID J:82825)
    • axonal swelling is observed in neurons in the amygdala and spinal cord of 3- to 6-month old mice; affected axoplasm is filled with numerous abnormally clustered mitochondria and lysosomes, and skeletal components are disorganized

Gene & Allele Details

Allele Symbol Tg(Eno2CDK5R1)1Jdm
Allele Name transgene insertion 1, John D McNeish
Common Name(s) NSE-p25 line A; P25;
Mutation Made By John McNeish,   Pfizer Central Research
Expressed Gene CDK5R1, cyclin-dependent kinase 5, regulatory subunit 1 (p35), human
Promoter Eno2, enolase 2, gamma, neuronal, rat
General Note A two-fold increase in the catalytic activity of Cdk5 is observed in transgenic mice. In contrast to wild type control mice, brains from 4-month-old exhibit the presence of tau and neurofilament phosphoepitopes. Ultrastructurally, axonal swelling in neurons from the amygdala and spinal cord is observed. Affected neurons possess numerous, abnormally clustered mitochondria and lysosomes, with some evidence of cytoskeletal disorganization. Mice exhibit whole-body tremors at 4-9 weeks of age and enhanced open field locomotor activity.
Molecular Note The transgene contains rat enolase 2, gamma neuronal (Eno2) promoter sequence and human cyclin-dependent kinase 5, regulatory subunit 1 sequence (CDK5R1) encoding the amino terminal proteolytic fragment p25. Robust expression of the transgenic fragment 25 is detected in the amygdala, thalamus/hypothalamus, cerebral cortex, and cerebellum. [MGI Ref ID J:82825]

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(Eno2CDK5R1)1Jdm

Colony Maintenance

Breeding & HusbandryThe donating investigator maintains this strain as a homozygote on a FVB/N background (N6).

Related Strains

Strains carrying other alleles of CDK5R1
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
View Strains carrying other alleles of CDK5R1     (1 strain)

View Strains carrying other alleles of Eno2     (8 strains)

Additional Web Information

Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Tremor Defects

References

Additional References

Price and Supply Information

Strain Name: FVB/N-Tg(Eno2CDK5R1)1Jdm/J
Stock Number: 003753

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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