Strain Name:

FVB/N-Tg(Eno2CDK5R1)1Jdm/J

Stock Number:

003753

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Michael K Ahlijanian,   Pfizer Central Research

Description
These transgenic animals display a robust expression of the amino terminal proteolytic fragment p25 of human CDK5R1. Expression is detected in the amygdala, thalamus/hypothalamus, cerebral cortex, and cerebellum. The p25 fragment acts as a positive allosteric regulator of cyclin-dependent kinase 5 (Cdk5), a kinase known to phosphorylate the microtubule-binding protein tau. A two-fold increase in the catalytic activity of Cdk5 is observed in these mice. In contrast to wildtype control mice, brains from 4-month-old exhibit the presence of tau and neurofilament phosphoepitopes. Utlrastructurally, axonal swelling in neurons from the amygdala and spinal cord is observed. Affected neurons possess numerous, abnormally clustered mitochondria and lysosomes, with some evidence of cytoskeletal disorganization. Mice exhibit whole-body tremors at 4-9 weeks of age and enhanced open-field locomotor activity. Aspects of this phenotype are similar to Alzheimer's, Pick's and other neurodegenerative diseases.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

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Strains carrying other alleles of CDK5R1
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View Strains carrying other alleles of CDK5R1     (2 strains)

View Strains carrying other alleles of Eno2     (12 strains)

Additional Web Information

Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Eno2CDK5R1)1Jdm/?

        involves: FVB/N
  • behavior/neurological phenotype
  • abnormal locomotor behavior
    • starting at 5-7 weeks of age, mice show increased open-field locomotor activity, which is 2-fold greater than wild-type at 16 weeks   (MGI Ref ID J:82825)
  • decreased anxiety-related response
    • mice show increased open-field locomotor activity, which is 2-fold greater than wild-type at 16 weeks and increased locomotor activity in activity chambers   (MGI Ref ID J:82825)
    • in elevated-plus maze, mice aged 9-12 weeks spend more time on the open arms compared to wild-type animals   (MGI Ref ID J:82825)
  • tremors
    • 4-9 week-old mice display whole-body exertion tremors   (MGI Ref ID J:82825)
  • nervous system phenotype
  • abnormal axon morphology
    • axonal swelling is observed in neurons in the amygdala and spinal cord of 3- to 6-month old mice; affected axoplasm is filled with numerous abnormally clustered mitochondria and lysosomes, and skeletal components are disorganized   (MGI Ref ID J:82825)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Neurodegeneration
Tremor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Eno2CDK5R1)1Jdm
Allele Name transgene insertion 1, John D McNeish
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) NSE-p25 line A; P25;
Mutation Made By John McNeish,   Pfizer Central Research
Expressed Gene CDK5R1, cyclin-dependent kinase 5, regulatory subunit 1 (p35), human
Promoter Eno2, enolase 2, gamma, neuronal, rat
General Note A two-fold increase in the catalytic activity of Cdk5 is observed in transgenic mice. In contrast to wild-type control mice, brains from 4-month-old exhibit the presence of tau and neurofilament phosphoepitopes. Ultrastructurally, axonal swelling in neurons from the amygdala and spinal cord is observed. Affected neurons possess numerous, abnormally clustered mitochondria and lysosomes, with some evidence of cytoskeletal disorganization. Mice exhibit whole-body tremors at 4-9 weeks of age and enhanced open field locomotor activity.
Molecular Note The transgene contains rat enolase 2, gamma neuronal (Eno2) promoter sequence and human cyclin-dependent kinase 5, regulatory subunit 1 sequence (CDK5R1) encoding the amino terminal proteolytic fragment p25. Robust expression of the transgenic fragment 25 is detected in the amygdala, thalamus/hypothalamus, cerebral cortex, and cerebellum. [MGI Ref ID J:82825]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Eno2CDK5R1)1Jdm, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Tg(Eno2CDK5R1)1Jdm related

Ahlijanian MK; Barrezueta NX; Williams RD; Jakowski A; Kowsz KP; McCarthy S; Coskran T; Carlo A; Seymour PA; Burkhardt JE; Nelson RB; McNeish JD. 2000. Hyperphosphorylated tau and neurofilament and cytoskeletal disruptions in mice overexpressing human p25, an activator of cdk5. Proc Natl Acad Sci U S A 97(6):2910-5. [PubMed: 10706614]  [MGI Ref ID J:82825]

Noble W; Olm V; Takata K; Casey E; Mary O; Meyerson J; Gaynor K; LaFrancois J; Wang L; Kondo T; Davies P; Burns M; Veeranna; Nixon R; Dickson D; Matsuoka Y; Ahlijanian M; Lau LF; Duff K. 2003. Cdk5 is a key factor in tau aggregation and tangle formation in vivo. Neuron 38(4):555-65. [PubMed: 12765608]  [MGI Ref ID J:130643]

Patrick GN; Zukerberg L; Nikolic M; de la Monte S; Dikkes P; Tsai LH. 1999. Conversion of p35 to p25 deregulates Cdk5 activity and promotes neurodegeneration. Nature 402(6762):615-22. [PubMed: 10604467]  [MGI Ref ID J:93866]

Wen Y; Yu WH; Maloney B; Bailey J; Ma J; Marie I; Maurin T; Wang L; Figueroa H; Herman M; Krishnamurthy P; Liu L; Planel E; Lau LF; Lahiri DK; Duff K. 2008. Transcriptional regulation of beta-secretase by p25/cdk5 leads to enhanced amyloidogenic processing. Neuron 57(5):680-90. [PubMed: 18341989]  [MGI Ref ID J:132937]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThe donating investigator maintains this strain as a homozygote on a FVB/N background (N6).

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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