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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6.129S4-Shroom3Gt(ROSA)53Sor/J (Changed: 05-SEP-11 ) B6.129S4-shrmGt(ROSA)53Sor/J (Changed: 24-JUL-06 ) shrmGtros (Changed: 15-DEC-04 ) Type Congenic; Gene Trap; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S4/SvJaeSor Generation req+N1p (29-FEB-04)
Generation DefinitionsAppearance
black
Related Genotype: a/aDescription
Mice that are homozygous for this mutation survive to term or die very shortly after birth. The gene trap insertion appears to have occurred between the translational start sites of the long and short forms in the 5' portion of the endogenous gene. No gene product (protein or mRNA) is detected in homozygous embryos. Mutant embryos can be distinguished by E9.25 as the lateral edges of the cranial neural folds exhibit a wavy appearance and fail to converge at the dorsal midline. As the embryo develops, the neural folds continue to enlarge and develop away from the dorsal midline, presenting a mushroom-like appearance. By day E14.5, failed neural tube closure results in exencephaly, acrania, and facial clefting. Some mutants exhibit defects in ventral closure resulting in herniation of the intestine and liver. Not all aspects of the phenotype are fully penetrant. Hemizygous mice express a B-galactosidase under the control of the endogenous promoter, with expression variously observed in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle.Development
A retroviral vector ROSA-BgalCre containing a gene trap cassette was introduced into AK7 embryonic stem (ES) cells. The components of ROSA-BgalCre include an adenovirus splice acceptor, a bifunctional gene fusion between B-galactosidase and Cre recombinase, and the MC1 polyadenylation sequence. ES cells containing gene trap insertions were used to generate chimeric mice. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J females.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (255 strains)
016157 129S1.B6-Shroom3m1Nisw/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Shroom3Gt(ROSA53)Sor/Shroom3+
involves: 129S4/SvJaeSor
- nervous system phenotype
- exencephaly
- observed in 8% of fetuses (MGI Ref ID J:58691)
Shroom3Gt(ROSA53)Sor/Shroom3Gt(ROSA53)Sor
involves: 129S4/SvJaeSor
- mortality/aging
- complete perinatal lethality
- mice survived to term, but were reported to most likely die during or shortly after birth (MGI Ref ID J:58691)
- craniofacial phenotype
- absent neurocranium
- evident at E14.5 and due to failed neural tube closure (MGI Ref ID J:58691)
- midline facial cleft
- digestive/alimentary phenotype
- herniated intestine
- embryogenesis phenotype
- abnormal embryogenesis/ development
- in spite of aberrant neural tube morphogenesis, the patterning and growth of surrounding tissues was largely unaffected (MGI Ref ID J:58691)
- open neural tube
- observed in 100% of mice (MGI Ref ID J:58691)
- neural folds "mushroom" outward and do not converge at the dorsal midline (MGI Ref ID J:58691)
- by E10.25 the neural tube is malformed along the anterior-posterior axis resulting in the exposure of the forebrain neuroepithelium (MGI Ref ID J:58691)
- 23% of fetuses exhibited failed caudal neural tube closure resulting in spina bifida (MGI Ref ID J:58691)
- surrounding tissues, including the notochord, floor plate, head mesenchyme, and somites, appear normal (MGI Ref ID J:58691)
- wavy neural tube
- evident at E9.25 (MGI Ref ID J:58691)
- skeleton phenotype
- absent neurocranium
- evident at E14.5 and due to failed neural tube closure (MGI Ref ID J:58691)
- nervous system phenotype
- exencephaly
- open neural tube
- observed in 100% of mice (MGI Ref ID J:58691)
- neural folds "mushroom" outward and do not converge at the dorsal midline (MGI Ref ID J:58691)
- by E10.25 the neural tube is malformed along the anterior-posterior axis resulting in the exposure of the forebrain neuroepithelium (MGI Ref ID J:58691)
- 23% of fetuses exhibited failed caudal neural tube closure resulting in spina bifida (MGI Ref ID J:58691)
- surrounding tissues, including the notochord, floor plate, head mesenchyme, and somites, appear normal (MGI Ref ID J:58691)
- wavy neural tube
- evident at E9.25 (MGI Ref ID J:58691)
- liver/biliary system phenotype
- herniated liver (MGI Ref ID J:58691)
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
Neural Tube Defects
Neurodevelopmental Defects
Perinatal Lethality
Homozygous
Neurobiology Research
Neural Tube Defects
Neurodevelopmental Defects
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Shroom3Gt(ROSA53)Sor | ||
|---|---|---|---|
| Allele Name | gene trap ROSA53, Philippe Soriano | ||
| Allele Type | Gene trapped | ||
| Common Name(s) | Shroom3Gt(ROSA)53Sor; ShroomGt; shrmGtrosa53; shroom; | ||
| Mutation Made By | Phillipe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | Expression of lacZ is detected in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | Gene trap insertion which disrupted expression of one of two alternative splice forms. Insertion occurred 3' of the translation start site for the longer transcript, but 5' to the translational start site for the shorter transcript. [MGI Ref ID J:58691] | ||
| Gene Symbol and Name | Shroom3, shroom family member 3 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AL022960; APXL3; D5Ertd287e; DNA segment, Chr 5, ERATO Doi 287, expressed; RGD1310470; SHRM; Shrm; Shrm3; ShrmL; expressed sequence AL022960; shroom; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hildebrand JD; Soriano P. 1999. Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell 99(5):485-97. [PubMed: 10589677] [MGI Ref ID J:58691]
Additional References
Shroom3Gt(ROSA53)Sor relatedDavidson CE; Li Q; Churchill GA; Osborne LR; McDermid HE. 2007. Modifier locus for exencephaly in Cecr2 mutant mice is syntenic to the 10q25.3 region associated with neural tube defects in humans. Physiol Genomics 31(2):244-51. [PubMed: 17623803] [MGI Ref ID J:127218]
Esterbauer H; Oberkofler H; Krempler F; Patsch W. 1999. Human peroxisome proliferator activated receptor gamma coactivator 1 (PPARGC1) gene: cDNA sequence, genomic organization, chromosomal localization, and tissue expression. Genomics 62(1):98-102. [PubMed: 10585775] [MGI Ref ID J:58961]
Grosse AS; Pressprich MF; Curley LB; Hamilton KL; Margolis B; Hildebrand JD; Gumucio DL. 2011. Cell dynamics in fetal intestinal epithelium: implications for intestinal growth and morphogenesis. Development 138(20):4423-32. [PubMed: 21880782] [MGI Ref ID J:178336]
Plageman TF Jr; Chung MI; Lou M; Smith AN; Hildebrand JD; Wallingford JB; Lang RA. 2010. Pax6-dependent Shroom3 expression regulates apical constriction during lens placode invagination. Development 137(3):405-15. [PubMed: 20081189] [MGI Ref ID J:156173]
Plageman TF Jr; Zacharias AL; Gage PJ; Lang RA. 2011. Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis. Dev Biol 357(1):227-34. [PubMed: 21726547] [MGI Ref ID J:175443]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129S background and has been backcrossed to C57BL/6J for at least ten generations. Homozygous mice are embryonic lethal.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Strain from the Neural Tube Defect Resource. First time use requires submission of a Request Form, please inquire.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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