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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Former Names B6.129S4-shrmGt(ROSA)53Sor/J (Changed: 24-JUL-06 ) shrmGtros (Changed: 15-DEC-04 ) Type Congenic; Gene Trap; Mutant Strain; Species laboratory mouse Background Strain C57BL/6J Donor Strain 129S4/SvJaeSor Generation req+N1p (29-FEB-04) Appearance
black
Related Genotype: a/aDescription
Mice that are homozygous for this mutation survive to term or die very shortly after birth. The gene trap insertion appears to have occurred between the translational start sites of the long and short forms in the 5' portion of the endogenous gene. No gene product (protein or mRNA) is detected in homozygous embryos. Mutant embryos can be distinguished by E9.25 as the lateral edges of the cranial neural folds exhibit a wavy appearance and fail to converge at the dorsal midline. As the embryo develops, the neural folds continue to enlarge and develop away from the dorsal midline, presenting a mushroom-like appearance. By day E14.5, failed neural tube closure results in exencephaly, acrania, and facial clefting. Some mutants exhibit defects in ventral closure resulting in herniation of the intestine and liver. Not all aspects of the phenotype are fully penetrant. Hemizygous mice express a B-galactosidase under the control of the endogenous promoter, with expression variously observed in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle.Development
A retroviral vector ROSA-BgalCre containing a gene trap cassette was introduced into AK7 embryonic stem (ES) cells. The components of ROSA-BgalCre include an adenovirus splice acceptor, a bifunctional gene fusion between B-galactosidase and Cre recombinase, and the MC1 polyadenylation sequence. ES cells containing gene trap insertions were used to generate chimeric mice. Resulting chimeric male animals were backcrossed to wildtype C57BL/6J females.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Internal/Organ Defects
Neural Tube Defects
Neurodevelopmental Defects
Perinatal Lethality (Homozygous)
Neurobiology Research
Neural Tube Defects
Neurodevelopmental Defects
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Shroom3Gt(ROSA)53Sor | ||
|---|---|---|---|
| Allele Name | gene trap 53, Philippe Soriano | ||
| Common Name(s) | shrmGtrosa53; shroom; | ||
| Mutation Made By | Phillipe Soriano, Fred Hutchinson Cancer Research Center | ||
| Strain of Origin | 129S4/SvJaeSor | ||
| ES Cell Line Name | AK7 | ||
| ES Cell Line Strain | 129S4/SvJaeSor | ||
| Site of Expression | Expression of lacZ is detected in the neural tube, forebrain, somites, ventral body wall, heart, gut and skeletal muscle. | ||
| Gene Symbol and Name | Shroom3, shroom family member 3 | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AL022960; APXL3; D5Ertd287e; DNA segment, Chr 5, ERATO Doi 287, expressed; KIAA1481; MSTP013; RGD1310470; SHRM; Shrm; Shrm3; ShrmL; expressed sequence AL022960; shroom; | ||
| Molecular Note | Gene trap insertion which disrupted expression of one of two alternative splice forms. Insertion occurred 3' of the translation start site for the longer transcript, but 5' to the translational start site for the shorter transcript. [MGI Ref ID J:58691] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Hildebrand JD; Soriano P. 1999. Shroom, a PDZ domain-containing actin-binding protein, is required for neural tube morphogenesis in mice. Cell 99(5):485-97. [PubMed: 10589677] [MGI Ref ID J:58691]
Additional References
Shroom3Gt(ROSA)53Sor related
Davidson CE; Li Q; Churchill GA; Osborne LR; McDermid HE. 2007. Modifier locus for exencephaly in Cecr2 mutant mice is syntenic to the 10q25.3 region associated with neural tube defects in humans. Physiol Genomics 31(2):244-51. [PubMed: 17623803] [MGI Ref ID J:127218]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129S background and has been backcrossed to C57BL/6J for at least ten generations. Homozygous mice are embryonic lethal.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact Information
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