Strain Name: |
B6.129S4-Meox2tm1(cre)Sor/J |
|---|---|
Stock Number: |
003755 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.129S4-Meox2CreSor (Changed: 15-DEC-04
) |
| Genes & Alleles | Meox2; Meox2tm1(cre)Sor; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Inbred x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Philippe Soriano, Fred Hutchinson Cancer Research Center Generation N6+N17 (08-JAN-08) Strain Description
This strain expresses Cre recombinase under the control of the endogenous Meox2 promoter. Expression of Cre recombinase is observed in epiblast-derived tissues as early as embryonic day 5. The insertion creates a null allele for the Meox2 gene. Homozygous mice are viable on this background but exhibit an overall reduction in muscle mass and the absence of specific muscles resulting in abnormal limb posture and reduced motility. This phenotype is variable. As many as 80% of homozygotes are severely affected, fail to thrive and die before weaning. Some homozygotes (10%) exhibit clefting of the secondary palate. These mice can be utilized as a deleter strain for loxP flanked DNA and provide an alternative to tetraploid embryo analysis.Strain Development
A targeting vector containing a nuclear localization sequence-modified Cre recombinase and neomycin resistance genes, linked to a bovine growth hormone polyadenylation was used to replace the first Meox coding exon and 300 base pairs of the following intron in AK7 embryonic stem (ES) cells. Correctly targeted ES cells were used to make chimeric animals which were backcrossed to C57BL/6 mice.
Gene & Allele Details
| Allele Symbol | Meox2tm1(cre)Sor | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Philippe Soriano | ||
| Common Name(s) | MORE; Meox2-Cre; Meox2Cre; Meox2CreSor; More-cre; Mox2 Cre; | ||
| Mutation Made By | Michelle Tallquist, UT Southwestern Medical Center | ||
| Site of Expression | epiblast-derived tissues as early as embryonic day 5; all primitive ectoderm by embryonic day 7 (but not endoderm or trophectoderm); null allele for Meox2 gene | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Meox2, mesenchyme homeobox 2 | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | AI528662; GAX; MOX2; Mox-2; expressed sequence AI528662; | ||
| Molecular Note | An nls-cre gene was inserted at the initiator ATG of the gene, followed by a neomycin selection cassette. The endogenous regulatory sequences drive expression by E5 in the epiblast and by E7 expression is detectable in all primitive ectoderm but not endoderm or trophectoderm. [J:105278] [J:132360] [J:60933] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Meox2cretm1(cre)Sor
Colony Maintenance
| Breeding & Husbandry | This strain originated on a B6;129S4/SvJaeSor background and has been backcrossed to C57BL/6 for at least six generations. Expected coat color from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Meox2
002807 B6C3Fe a/a-Meox2fla/J View Strains carrying other alleles of Meox2 (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox-System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox system)
Meox2tm1(cre)Sor relatedResearch Tools
Cre-lox-System
Developmental Biology Research
Mesodermal Defects
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesTallquist MD; Soriano P. 2000. Epiblast-restricted Cre expression in MORE mice: a tool to distinguish embryonic vs. extra-embryonic gene function. Genesis 26(2):113-5. [PubMed: 10686601] [J:60933]
Price and Supply Information
| Strain Name: | B6.129S4-Meox2tm1(cre)Sor/J |
| Stock Number: | 003755 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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