Strain Name: |
B6.Cg-Tg(tetFosb)4468Nes/J |
|---|---|
Stock Number: |
003762 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Fosb; Tg(tetFosb)4468Nes; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Eric Nestler, UT Southwestern Medical Center Generation N8 Strain Description
Mice homozygous for the Fosb transgene are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing tetracycline-controlled transactivator protein (tTA or rtTA) to create a bitransgenic animal, Fosb expression can be regulated with the tetracycline analog, doxycycline. This transgenic strain can be crossed with strains 003763 or 003767 to produce bitransgenic mice that can be induced to express high levels of the Fosb variant in brain tissue by withdrawing doxycycline.Strain Development
A transgenic construct containing a Fosb cDNA under the control of a tetracycline-regulated promoter (TRE; tetO), an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6 x SJL hybrid mice. Founder mice were mated to ICR mice and then backcrossed to C57BL/6J mice.
Gene & Allele Details
| Allele Symbol | Tg(tetFosb)4468Nes | ||
|---|---|---|---|
| Allele Name | transgene insertion 4468, Eric Nestler | ||
| Common Name(s) | TetOp-*FosB line 11; TetOp-deltaFosB line 11; | ||
| Mutation Made By | Eric Nestler, UT Southwestern Medical Center | ||
| Strain of Origin | (SJL x C57BL/6)F1 | ||
| Site of Expression | When mated to a strain expressing tTA, Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. | ||
| Expressed Gene | Fosb, FBJ osteosarcoma oncogene B, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| General Note | Homozygous transgenic mice are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE). In bitransgenic mice produced by breeding a mouse carrying this transgene with a transgenic mouse expressing tetracycline-controlled transactivator protein (tTA), Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. | ||
| Molecular Note | The transgene contains a Fosb cDNA under the control of a tetracycline-regulated promoter, an SV40 intron, and a poly A signal sequence. [MGI Ref ID J:92225] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(tetFosb)4468Nes
Colony Maintenance
| Breeding & Husbandry | This strain originated on a B6;SJL background. Founder mice were crossed to ICR before being backcrossed to C57BL/6J (N6 7/20/00) |
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Related Strains
Strains carrying other alleles of Fosb
003077 C;129S-Fosbtm1Meg/J View Strains carrying other alleles of Fosb (1 strain)
Additional Web Information
Congenic Nomenclature
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Research Tools
Tet Expression Systems (tTA/rtTA Responsive Strains)
References
Selected Reference(s)
Additional ReferencesChen J; Kelz MB; Zeng G; Sakai N; Steffen C; Shockett PE; Picciotto MR; Duman RS; Nestler EJ. 1998. Transgenic animals with inducible, targeted gene expression in brain. Mol Pharmacol 54(3):495-503. [PubMed: 9730908] [MGI Ref ID J:92225]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(tetFosb)4468Nes/J |
| Stock Number: | 003762 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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