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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N8 Donating Investigator Eric Nestler, UT Southwestern Medical Center Description
Mice homozygous for the Fosb transgene are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing tetracycline-controlled transactivator protein (tTA or rtTA) to create a bitransgenic animal, Fosb expression can be regulated with the tetracycline analog, doxycycline. This transgenic strain can be crossed with strains 003763 or 003767 to produce bitransgenic mice that can be induced to express high levels of the Fosb variant in brain tissue by withdrawing doxycycline.Development
A transgenic construct containing a Fosb cDNA under the control of a tetracycline-regulated promoter (TRE; tetO), an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6 x SJL hybrid mice. Founder mice were mated to ICR mice and then backcrossed to C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Fosb
003077 C;129S-Fosbtm1Meg/J View Strains carrying other alleles of Fosb (1 strain)
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
Research Tools
Tet Expression Systems
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetFosb)4468Nes | ||
|---|---|---|---|
| Allele Name | transgene insertion 4468, Eric Nestler | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TetOp-*FosB line 11; TetOp-deltaFosB line 11; | ||
| Mutation Made By | Eric Nestler, UT Southwestern Medical Center | ||
| Strain of Origin | (SJL x C57BL/6)F1 | ||
| Site of Expression | When mated to a strain expressing tTA, Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. | ||
| Expressed Gene | Fosb, FBJ osteosarcoma oncogene B, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| General Note | Homozygous transgenic mice are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE). In bitransgenic mice produced by breeding a mouse carrying this transgene with a transgenic mouse expressing tetracycline-controlled transactivator protein (tTA), Fosb expression can be negatively regulated with the tetracycline analog, doxycycline. | ||
| Molecular Note | The transgene contains a Fosb cDNA under the control of a tetracycline-regulated promoter, an SV40 intron, and a poly A signal sequence. [MGI Ref ID J:92225] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tetFosb)4468Nes, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Chen J; Kelz MB; Zeng G; Sakai N; Steffen C; Shockett PE; Picciotto MR; Duman RS; Nestler EJ. 1998. Transgenic animals with inducible, targeted gene expression in brain. Mol Pharmacol 54(3):495-503. [PubMed: 9730908] [MGI Ref ID J:92225]
Additional References
McClung CA; Nestler EJ. 2003. Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci 6(11):1208-15. [PubMed: 14566342] [MGI Ref ID J:92942]
Sabatakos G; Sims NA; Chen J; Aoki K; Kelz MB; Amling M; Bouali Y; Mukhopadhyay K; Ford K; Nestler EJ; Baron R. 2000. Overexpression of DeltaFosB transcription factor(s) increases bone formation and inhibits adipogenesis Nat Med 6(9):985-90. [PubMed: 10973317] [MGI Ref ID J:64495]
Tg(tetFosb)4468Nes relatedColby CR; Whisler K; Steffen C; Nestler EJ; Self DW. 2003. Striatal cell type-specific overexpression of DeltaFosB enhances incentive for cocaine. J Neurosci 23(6):2488-93. [PubMed: 12657709] [MGI Ref ID J:82666]
Kelz MB; Chen J; Carlezon WA Jr; Whisler K; Gilden L; Beckmann AM; Steffen C; Zhang YJ; Marotti L; Self DW; Tkatch T; Baranauskas G; Surmeier DJ; Neve RL; Duman RS; Picciotto MR; Nestler EJ. 1999. Expression of the transcription factor deltaFosB in the brain controls sensitivity to cocaine. Nature 401(6750):272-6. [PubMed: 10499584] [MGI Ref ID J:92946]
Kelz MB; Kuszak JR; Yang Y; Ma W; Steffen C; Al-Ghoul K; Zhang YJ; Chen J; Nestler EJ; Spector A. 2000. DeltaFosB-induced cataract. Invest Ophthalmol Vis Sci 41(11):3523-38. [PubMed: 11006248] [MGI Ref ID J:64729]
Kumar A; Choi KH; Renthal W; Tsankova NM; Theobald DE; Truong HT; Russo SJ; Laplant Q; Sasaki TS; Whistler KN; Neve RL; Self DW; Nestler EJ. 2005. Chromatin remodeling is a key mechanism underlying cocaine-induced plasticity in striatum. Neuron 48(2):303-14. [PubMed: 16242410] [MGI Ref ID J:107584]
McClung CA; Nestler EJ. 2003. Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci 6(11):1208-15. [PubMed: 14566342] [MGI Ref ID J:92942]
Olausson P; Jentsch JD; Tronson N; Neve RL; Nestler EJ; Taylor JR. 2006. DeltaFosB in the nucleus accumbens regulates food-reinforced instrumental behavior and motivation. J Neurosci 26(36):9196-204. [PubMed: 16957076] [MGI Ref ID J:144670]
Rowe GC; Choi CS; Neff L; Horne WC; Shulman GI; Baron R. 2009. Increased energy expenditure and insulin sensitivity in the high bone mass DeltaFosB transgenic mice. Endocrinology 150(1):135-43. [PubMed: 18772235] [MGI Ref ID J:146998]
Sabatakos G; Sims NA; Chen J; Aoki K; Kelz MB; Amling M; Bouali Y; Mukhopadhyay K; Ford K; Nestler EJ; Baron R. 2000. Overexpression of DeltaFosB transcription factor(s) increases bone formation and inhibits adipogenesis Nat Med 6(9):985-90. [PubMed: 10973317] [MGI Ref ID J:64495]
Sims NA; Sabatakos G; Chen JS; Kelz MB; Nestler EJ; Baron R. 2002. Regulating DeltaFosB expression in adult Tet-Off-DeltaFosB transgenic mice alters bone formation and bone mass. Bone 30(1):32-9. [PubMed: 11792562] [MGI Ref ID J:130653]
Zachariou V; Bolanos CA; Selley DE; Theobald D; Cassidy MP; Kelz MB; Shaw-Lutchman T; Berton O; Sim-Selley LJ; Dileone RJ; Kumar A; Nestler EJ. 2006. An essential role for DeltaFosB in the nucleus accumbens in morphine action. Nat Neurosci 9(2):205-211. [PubMed: 16415864] [MGI Ref ID J:105317]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;SJL background. Founder mice were crossed to ICR before being backcrossed to C57BL/6J (N6 7/20/00)
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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JAX® Mice, Products & Services Conditions of Use
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