Strain Name:

B6.Cg-Tg(tetFosb)4468Nes/J

Stock Number:

003762

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN8
 
Donating Investigator Eric Nestler,   UT Southwestern Medical Center

Description
Mice homozygous for the Fosb transgene are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE; tetO). When hemizygotes are bred with another transgenic mouse expressing tetracycline-controlled transactivator protein (tTA or rtTA) to create a bitransgenic animal, Fosb expression can be regulated with the tetracycline analog, doxycycline. This transgenic strain can be crossed with strains 003763 or 003767 to produce bitransgenic mice that can be induced to express high levels of the Fosb variant in brain tissue by withdrawing doxycycline.

Development
A transgenic construct containing a Fosb cDNA under the control of a tetracycline-regulated promoter (TRE; tetO), an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6 x SJL hybrid mice. Founder mice were mated to ICR mice and then backcrossed to C57BL/6J mice.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Fosb
003077   C;129S-Fosbtm1Meg/J
View Strains carrying other alleles of Fosb     (1 strain)

Strains carrying other alleles of tetO
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
007051   B6.Cg-Tg(tetO-APPSwInd)102Dbo/J
007052   B6.Cg-Tg(tetO-APPSwInd)107Dbo/J
007049   B6.Cg-Tg(tetO-APPSwInd)885Dbo/J
007618   B6.Cg-Tg(tetO-Arntl)1Jt/J
008277   B6.Cg-Tg(tetO-Clockm1Jt)CL57Jt/J
008468   B6.Cg-Tg(tetO-DTA)1Gfi/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
010577   B6;SJL-Tg(tetO-Erbb2*)8-4Jek/J
002621   B6;SJL-Tg(tetop-lacZ)2Mam/J
006004   B6C3-Tg(tetO-APPSwInd)885Dbo/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
008278   C57BL/6J-Tg(tetO-Clock)1Jt/J
010578   FVB-Tg(tetO-Dusp6)1Jmol/J
008685   FVB-Tg(tetO-Kdr*)4377.5Rwng/J
008695   FVB-Tg(tetO-MET)23Rwng/J
006439   FVB-Tg(tetO/CMV-KRAS*G12C)9.1Msmi/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
005941   FVB/N-Tg(tetO-Aurkb,lacZ)41Kra/J
006202   FVB/N-Tg(tetO-BCR/ABL1)2Dgt/J
003315   FVB/N-Tg(tetORo1-lacZ)3Conk/J
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J
008755   STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J
008790   STOCK Tg(tetO-DISC1*)1001Plet/J
008168   STOCK Tg(tetO-DTA)1Gfi/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
005728   STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of tetO     (36 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Behavioral and Learning Defects

Research Tools
Tet Expression Systems
      tTA/rtTA Responsive Strains

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(tetFosb)4468Nes
Allele Name transgene insertion 4468, Eric Nestler
Allele Type Transgenic (random, expressed)
Common Name(s) TetOp-*FosB line 11; TetOp-deltaFosB line 11;
Mutation Made By Eric Nestler,   UT Southwestern Medical Center
Strain of Origin(SJL x C57BL/6)F1
Site of ExpressionWhen mated to a strain expressing tTA, Fosb expression can be negatively regulated with the tetracycline analog, doxycycline.
Expressed Gene Fosb, FBJ osteosarcoma oncogene B, mouse, laboratory
Promoter tetO, tet operator,
General Note Homozygous transgenic mice are viable and fertile. These mice express a truncated variant of the Fosb transcription factor. The expressed variant is a highly stable isoform found to be induced in the brain (nucleus accumbens) with repeated exposure to commonly abused drugs, such as amphetamine, morphine, nicotine, and phencyclidine. Transcription is directed by a tetracycline-responsive promoter element (TRE). In bitransgenic mice produced by breeding a mouse carrying this transgene with a transgenic mouse expressing tetracycline-controlled transactivator protein (tTA), Fosb expression can be negatively regulated with the tetracycline analog, doxycycline.
Molecular Note The transgene contains a Fosb cDNA under the control of a tetracycline-regulated promoter, an SV40 intron, and a poly A signal sequence. [MGI Ref ID J:92225]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tetFosb)4468Nes, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Chen J; Kelz MB; Zeng G; Sakai N; Steffen C; Shockett PE; Picciotto MR; Duman RS; Nestler EJ. 1998. Transgenic animals with inducible, targeted gene expression in brain. Mol Pharmacol 54(3):495-503. [PubMed: 9730908]  [MGI Ref ID J:92225]

Additional References

McClung CA; Nestler EJ. 2003. Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci 6(11):1208-15. [PubMed: 14566342]  [MGI Ref ID J:92942]

Sabatakos G; Sims NA; Chen J; Aoki K; Kelz MB; Amling M; Bouali Y; Mukhopadhyay K; Ford K; Nestler EJ; Baron R. 2000. Overexpression of DeltaFosB transcription factor(s) increases bone formation and inhibits adipogenesis Nat Med 6(9):985-90. [PubMed: 10973317]  [MGI Ref ID J:64495]

Tg(tetFosb)4468Nes related

Colby CR; Whisler K; Steffen C; Nestler EJ; Self DW. 2003. Striatal cell type-specific overexpression of DeltaFosB enhances incentive for cocaine. J Neurosci 23(6):2488-93. [PubMed: 12657709]  [MGI Ref ID J:82666]

Kelz MB; Chen J; Carlezon WA Jr; Whisler K; Gilden L; Beckmann AM; Steffen C; Zhang YJ; Marotti L; Self DW; Tkatch T; Baranauskas G; Surmeier DJ; Neve RL; Duman RS; Picciotto MR; Nestler EJ. 1999. Expression of the transcription factor deltaFosB in the brain controls sensitivity to cocaine. Nature 401(6750):272-6. [PubMed: 10499584]  [MGI Ref ID J:92946]

Kelz MB; Kuszak JR; Yang Y; Ma W; Steffen C; Al-Ghoul K; Zhang YJ; Chen J; Nestler EJ; Spector A. 2000. DeltaFosB-induced cataract. Invest Ophthalmol Vis Sci 41(11):3523-38. [PubMed: 11006248]  [MGI Ref ID J:64729]

Kumar A; Choi KH; Renthal W; Tsankova NM; Theobald DE; Truong HT; Russo SJ; Laplant Q; Sasaki TS; Whistler KN; Neve RL; Self DW; Nestler EJ. 2005. Chromatin remodeling is a key mechanism underlying cocaine-induced plasticity in striatum. Neuron 48(2):303-14. [PubMed: 16242410]  [MGI Ref ID J:107584]

McClung CA; Nestler EJ. 2003. Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci 6(11):1208-15. [PubMed: 14566342]  [MGI Ref ID J:92942]

Olausson P; Jentsch JD; Tronson N; Neve RL; Nestler EJ; Taylor JR. 2006. DeltaFosB in the nucleus accumbens regulates food-reinforced instrumental behavior and motivation. J Neurosci 26(36):9196-204. [PubMed: 16957076]  [MGI Ref ID J:144670]

Rowe GC; Choi CS; Neff L; Horne WC; Shulman GI; Baron R. 2009. Increased energy expenditure and insulin sensitivity in the high bone mass DeltaFosB transgenic mice. Endocrinology 150(1):135-43. [PubMed: 18772235]  [MGI Ref ID J:146998]

Sabatakos G; Sims NA; Chen J; Aoki K; Kelz MB; Amling M; Bouali Y; Mukhopadhyay K; Ford K; Nestler EJ; Baron R. 2000. Overexpression of DeltaFosB transcription factor(s) increases bone formation and inhibits adipogenesis Nat Med 6(9):985-90. [PubMed: 10973317]  [MGI Ref ID J:64495]

Sims NA; Sabatakos G; Chen JS; Kelz MB; Nestler EJ; Baron R. 2002. Regulating DeltaFosB expression in adult Tet-Off-DeltaFosB transgenic mice alters bone formation and bone mass. Bone 30(1):32-9. [PubMed: 11792562]  [MGI Ref ID J:130653]

Zachariou V; Bolanos CA; Selley DE; Theobald D; Cassidy MP; Kelz MB; Shaw-Lutchman T; Berton O; Sim-Selley LJ; Dileone RJ; Kumar A; Nestler EJ. 2006. An essential role for DeltaFosB in the nucleus accumbens in morphine action. Nat Neurosci 9(2):205-211. [PubMed: 16415864]  [MGI Ref ID J:105317]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;SJL background. Founder mice were crossed to ICR before being backcrossed to C57BL/6J (N6 7/20/00)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

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