Strain Name:

B6.Cg-Tg(Eno2tTA)5030Nes/J

Stock Number:

003763

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain mixed stock
GenerationN7+5pN1
 
Donating Investigator Eric Nestler,   UT Southwestern Medical Center

Appearance
black
Related Genotype: a/a

Description
These transgenic mice express the tetracycline-controlled activator protein (tTA) under the control of a rat neuron-specific enolase (Eno2) promoter. Although the Eno2 promoter has been shown to direct high levels of expression in brain tissue in general, this strain selectively expresses tTA at high levels in the striatum and to a lesser extent in the cerebral cortex and hippocampus. When these transgenic mice are mated to a second transgenic strain carrying a gene of interest under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring may be induced in the tTA-expressing tissues by the withdrawl of the tetracycline analog, doxycycline (dox). For example, when these transgenic mice are bred with Stock No. 003762, a transgenic strain that expresses a Fosb variant under the direction of a TRE, the resulting bitransgenic offspring can be induced to express high levels of the Fosb variant in the striatum by withdrawing dox.

Development
A transgenic construct containing a modified tTa cDNA under the control of an Eno2 (Enolase 2, gamma, neuronal) promoter, an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6;SJL mice. Founder mice (line A in the primary reference) were mated to ICR mice and then backcrossed to C57BL/6J mice.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Eno2     (9 strains)

View Strains carrying other alleles of tTA     (19 strains)

Additional Web Information

Congenic Nomenclature
Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Tet Expression System (tTA/rtTA Expressing Strains)

Research Tools
Neurobiology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Eno2tTA)5030Nes
Allele Name transgene insertion 5030, Eric Nestler
Allele Type Transgenic (random, expressed)
Common Name(s) A line; NSE-tTA; Tg(Eno2-tTA)ANes;
Mutation Made By Eric Nestler,   UT Southwestern Medical Center
Strain of Origin(SJL x C57BL/6)F1
Site of ExpressionExpresses tTA at high levels in the striatum.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Eno2, enolase 2, gamma, neuronal, rat
General Note B6.Cg-TgN(Eno2tTA)5030Nes mice selectively express tTA at high levels in the striatum and to a lesser extent in the cerebral cortex and hippocampus. When these transgenic mice are mated to a second transgenic strain which carries a gene of interest underthe regulation of a tetracycline-responsive promoter element (TRE), expression of the target gene in the bitransgenic offspring may be induced in those tissues where tTA is expressed by the withdrawal of the tetracycline analog, doxycycline (dox).
Molecular Note This transgene consists of a 1.8kb fragment of the rat Eno2 promoter, an SV40 intron, a poly A signal sequence, and a modified tetracycline transactivator cDNA. [MGI Ref ID J:92225]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(tTA), MCA, vers. 4
Tg(tTA), STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Chen J; Kelz MB; Zeng G; Sakai N; Steffen C; Shockett PE; Picciotto MR; Duman RS; Nestler EJ. 1998. Transgenic animals with inducible, targeted gene expression in brain. Mol Pharmacol 54(3):495-503. [PubMed: 9730908]  [MGI Ref ID J:92225]

Additional References

McClung CA; Nestler EJ. 2003. Regulation of gene expression and cocaine reward by CREB and DeltaFosB. Nat Neurosci 6(11):1208-15. [PubMed: 14566342]  [MGI Ref ID J:92942]

Sabatakos G; Sims NA; Chen J; Aoki K; Kelz MB; Amling M; Bouali Y; Mukhopadhyay K; Ford K; Nestler EJ; Baron R. 2000. Overexpression of DeltaFosB transcription factor(s) increases bone formation and inhibits adipogenesis Nat Med 6(9):985-90. [PubMed: 10973317]  [MGI Ref ID J:64495]

Tg(Eno2tTA)5030Nes related

Chen J; Kelz MB; Zeng G; Steffen C; Shockett PE; Terwilliger G; Schatz DG; Nestler EJ. 2002. Inducible, reversible hair loss in transgenic mice. Transgenic Res 11(3):241-7. [PubMed: 12113456]  [MGI Ref ID J:77497]

Colby CR; Whisler K; Steffen C; Nestler EJ; Self DW. 2003. Striatal cell type-specific overexpression of DeltaFosB enhances incentive for cocaine. J Neurosci 23(6):2488-93. [PubMed: 12657709]  [MGI Ref ID J:82666]

Eckenstein FP; McGovern T; Kern D; Deignan J. 2006. Neuronal vulnerability in transgenic mice expressing an inducible dominant-negative FGF receptor. Exp Neurol 198(2):338-49. [PubMed: 16487970]  [MGI Ref ID J:107899]

Fujioka T; Fujioka A; Duman RS. 2004. Activation of cAMP signaling facilitates the morphological maturation of newborn neurons in adult hippocampus. J Neurosci 24(2):319-28. [PubMed: 14724230]  [MGI Ref ID J:87734]

Hashimoto T; Bergen SE; Nguyen QL; Xu B; Monteggia LM; Pierri JN; Sun Z; Sampson AR; Lewis DA. 2005. Relationship of brain-derived neurotrophic factor and its receptor TrkB to altered inhibitory prefrontal circuitry in schizophrenia. J Neurosci 25(2):372-83. [PubMed: 15647480]  [MGI Ref ID J:97675]

Hill JJ; Kolluri N; Hashimoto T; Wu Q; Sampson AR; Monteggia LM; Lewis DA. 2005. Analysis of pyramidal neuron morphology in an inducible knockout of brain-derived neurotrophic factor. Biol Psychiatry 57(8):932-4. [PubMed: 15820715]  [MGI Ref ID J:102119]

Kelz MB; Chen J; Carlezon WA Jr; Whisler K; Gilden L; Beckmann AM; Steffen C; Zhang YJ; Marotti L; Self DW; Tkatch T; Baranauskas G; Surmeier DJ; Neve RL; Duman RS; Picciotto MR; Nestler EJ. 1999. Expression of the transcription factor deltaFosB in the brain controls sensitivity to cocaine. Nature 401(6750):272-6. [PubMed: 10499584]  [MGI Ref ID J:92946]

Kelz MB; Kuszak JR; Yang Y; Ma W; Steffen C; Al-Ghoul K; Zhang YJ; Chen J; Nestler EJ; Spector A. 2000. DeltaFosB-induced cataract. Invest Ophthalmol Vis Sci 41(11):3523-38. [PubMed: 11006248]  [MGI Ref ID J:64729]

King SL; Marks MJ; Grady SR; Caldarone BJ; Koren AO; Mukhin AG; Collins AC; Picciotto MR. 2003. Conditional expression in corticothalamic efferents reveals a developmental role for nicotinic acetylcholine receptors in modulation of passive avoidance behavior. J Neurosci 23(9):3837-43. [PubMed: 12736354]  [MGI Ref ID J:123821]

Monteggia LM; Barrot M; Powell CM; Berton O; Galanis V; Gemelli T; Meuth S; Nagy A; Greene RW; Nestler EJ. 2004. Essential role of brain-derived neurotrophic factor in adult hippocampal function. Proc Natl Acad Sci U S A 101(29):10827-32. [PubMed: 15249684]  [MGI Ref ID J:91563]

Newton SS; Thome J; Wallace TL; Shirayama Y; Schlesinger L; Sakai N; Chen J; Neve R; Nestler EJ; Duman RS. 2002. Inhibition of cAMP response element-binding protein or dynorphin in the nucleus accumbens produces an antidepressant-like effect. J Neurosci 22(24):10883-90. [PubMed: 12486182]  [MGI Ref ID J:128618]

Paletzki RF; Myakishev MV; Polesskaya O; Orosz A; Hyman SE; Vinson C. 2008. Inhibiting activator protein-1 activity alters cocaine-induced gene expression and potentiates sensitization. Neuroscience 152(4):1040-53. [PubMed: 18355967]  [MGI Ref ID J:136074]

Peakman MC; Colby C; Perrotti LI; Tekumalla P; Carle T; Ulery P; Chao J; Duman C; Steffen C; Monteggia L; Allen MR; Stock JL; Duman RS; McNeish JD; Barrot M; Self DW; Nestler EJ; Schaeffer E. 2003. Inducible, brain region-specific expression of a dominant negative mutant of c-Jun in transgenic mice decreases sensitivity to cocaine. Brain Res 970(1-2):73-86. [PubMed: 12706249]  [MGI Ref ID J:83308]

Sabatakos G; Sims NA; Chen J; Aoki K; Kelz MB; Amling M; Bouali Y; Mukhopadhyay K; Ford K; Nestler EJ; Baron R. 2000. Overexpression of DeltaFosB transcription factor(s) increases bone formation and inhibits adipogenesis Nat Med 6(9):985-90. [PubMed: 10973317]  [MGI Ref ID J:64495]

Sakai N; Thome J; Newton SS; Chen J; Kelz MB; Steffen C; Nestler EJ; Duman RS. 2002. Inducible and brain region-specific CREB transgenic mice. Mol Pharmacol 61(6):1453-64. [PubMed: 12021407]  [MGI Ref ID J:92951]

Sims NA; Sabatakos G; Chen JS; Kelz MB; Nestler EJ; Baron R. 2002. Regulating DeltaFosB expression in adult Tet-Off-DeltaFosB transgenic mice alters bone formation and bone mass. Bone 30(1):32-9. [PubMed: 11792562]  [MGI Ref ID J:130653]

Zachariou V; Bolanos CA; Selley DE; Theobald D; Cassidy MP; Kelz MB; Shaw-Lutchman T; Berton O; Sim-Selley LJ; Dileone RJ; Kumar A; Nestler EJ. 2006. An essential role for DeltaFosB in the nucleus accumbens in morphine action. Nat Neurosci 9(2):205-211. [PubMed: 16415864]  [MGI Ref ID J:105317]

Zeng H; Horie K; Madisen L; Pavlova MN; Gragerova G; Rohde AD; Schimpf BA; Liang Y; Ojala E; Kramer F; Roth P; Slobodskaya O; Dolka I; Southon EA; Tessarollo L; Bornfeldt KE; Gragerov A; Pavlakis GN; Gaitanaris GA. 2008. An inducible and reversible mouse genetic rescue system. PLoS Genet 4(5):e1000069. [PubMed: 18464897]  [MGI Ref ID J:136987]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;SJL background. Founder mice were crossed to ICR before being backcrossed to C57BL/6J (N7 7/20/00) Coat color expected from breeding:Black
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


(3.2)