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Strain Name:

B6.Cg-Tg(Eno2tTA)5030Nes/J

Stock Number:

003763

Availability:

Repository-Cryopreserved

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Genes & Alleles   Eno2;   tTA;   Tg(Eno2tTA)5030Nes;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain mixed stock
Donating Investigator Eric Nestler,   UT Southwestern Medical Center
GenerationN7+5pN1

Appearance
black
Related Genotype: a/a

Strain Description
These transgenic mice express the tetracycline-controlled activator protein (tTA) under the control of a rat neuron-specific enolase (Eno2) promoter. Although the Eno2 promoter has been shown to direct high levels of expression in brain tissue in general, this strain selectively expresses tTA at high levels in the striatum and to a lesser extent in the cerebral cortex and hippocampus. When these transgenic mice are mated to a second transgenic strain carrying a gene of interest under the regulation of a tetracycline-responsive promoter element (TRE; tetO), expression of the target gene in the bitransgenic offspring may be induced in the tTA-expressing tissues by the withdrawl of the tetracycline analog, doxycycline (dox). For example, when these transgenic mice are bred with Stock No. 003762, a transgenic strain that expresses a Fosb variant under the direction of a TRE, the resulting bitransgenic offspring can be induced to express high levels of the Fosb variant in the striatum by withdrawing dox.

Strain Development
A transgenic construct containing a modified tTa cDNA under the control of an Eno2 (Enolase 2, gamma, neuronal) promoter, an SV40 intron and a poly A signal sequence, was injected into the pronuclei of oocytes from B6;SJL mice. Founder mice (line A in the primary reference) were mated to ICR mice and then backcrossed to C57BL/6J mice.

Gene & Allele Details

Allele Symbol Tg(Eno2tTA)5030Nes
Allele Name transgene insertion 5030, Eric Nestler
Common Name(s) A line; NSE-tTA; Tg(Eno2-tTA)ANes;
Mutation Made By Eric Nestler,   UT Southwestern Medical Center
Strain of Origin(SJL x C57BL/6)F1
Site of ExpressionExpresses tTA at high levels in the striatum.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Eno2, enolase 2, gamma, neuronal, rat
General Note B6.Cg-TgN(Eno2tTA)5030Nes mice selectively express tTA at high levels in the striatum and to a lesser extent in the cerebral cortex and hippocampus. When these transgenic mice are mated to a second transgenic strain which carries a gene of interest underthe regulation of a tetracycline-responsive promoter element (TRE), expression of the target gene in the bitransgenic offspring may be induced in those tissues where tTA is expressed by the withdrawal of the tetracycline analog, doxycycline (dox).
Molecular Note This transgene consists of a 1.8kb fragment of the rat Eno2 promoter, an SV40 intron, a poly A signal sequence, and a modified tetracycline transactivator cDNA. [MGI Ref ID J:92225]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(tTA)

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;SJL background. Founder mice were crossed to ICR before being backcrossed to C57BL/6J (N7 7/20/00) Coat color expected from breeding:Black
Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of Eno2     (8 strains)

View Strains carrying other alleles of tTA     (19 strains)

Additional Web Information

Congenic Nomenclature
Tet Expression Systems

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Tet Expression System (tTA/rtTA Expressing Strains)

Research Tools
Neurobiology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

Chen J; Kelz MB; Zeng G; Sakai N; Steffen C; Shockett PE; Picciotto MR; Duman RS; Nestler EJ. 1998. Transgenic animals with inducible, targeted gene expression in brain. Mol Pharmacol 54(3):495-503. [PubMed: 9730908]  [MGI Ref ID J:92225]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(Eno2tTA)5030Nes/J
Stock Number: 003763

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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