Description

Strain Information

Former Names B6.129S4-Trpv1tm1Jul/J    (Changed: 18-OCT-05 ) B6.129S4-Vr1tm1Jul    (Changed: 15-DEC-04 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation N10F12 (20-DEC-06)   Donating Investigator David Julius,   Univ of California at San Francisco

Description
Mice that are homozygous targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected in dorsal root ganglia. Cultured dorsal root ganglia neurons and skin preparations display no, or markedly attenuated, response to vanilloid compounds, acidified environments or heat (43 degrees C). In intact wild type mice, a subcutaneous injection of vanilloid compounds into the hind paw elicits a pain response with subsequent swelling. No pain response is observed in homozygotes and swelling is noticeably reduced. Also absent is the profound reduction in body temperature following a subcutaneous injection of capsaicin. Homozygotes appear to display robust deficits in thermally evoked pain-related behavior and do not display an aversion to ingesting capsaicin-supplemented drinking water.

Development
A targeting vector was used to disrupt an exon encoding part of the fifth and all of the sixth putative transmembrane domains together with the pore-loop region. The construct was electroporated into 129X1/SvJ-derived JM1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 female mice, and then backcrossed to the same for 10 generations.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Trpv1^tm1Jul/Trpv1^tm1Jul

        involves: C57BL/6

• renal/urinary system phenotype
• abnormal urination (MGI Ref ID J:78707)
  • increased frequency of non-voiding contractions
  • the frequency of and strength voiding contractions were similar those of wild-type littermates

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Trpv1^tm1Jul/Trpv1^tm1Jul

        Background Not Specified

• behavior/neurological phenotype
• abnormal nociception after inflammation (MGI Ref ID J:61534)
  • reduced thermal hypersensitvitiy following inflammation
• increased thermal nociceptive threshold (MGI Ref ID J:61534)
  • impaired detection of painful heat
• touch/vibrissae phenotype
• abnormal nociception after inflammation (MGI Ref ID J:61534)
  • reduced thermal hypersensitvitiy following inflammation
• increased thermal nociceptive threshold (MGI Ref ID J:61534)
  • impaired detection of painful heat

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Trpv1^tm1Jul related

Immunology and Inflammation Research
Inflammation

Neurobiology Research
Receptor Defects

Sensorineural Research
Nociception

Genes & Alleles

Gene & Allele Information

Allele Symbol		Trpv1tm1Jul
Allele Name		targeted mutation 1, David Julius
Allele Type		Targeted (knock-out)
Common Name(s)		Vr1tm1Jul; trpv1-;
Mutation Made By		David Julius,   Univ of California at San Francisco
Strain of Origin		129X1/SvJ
ES Cell Line Name		JM-1
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Trpv1, transient receptor potential cation channel, subfamily V, member 1
Chromosome		11
Gene Common Name(s)		DKFZp434K0220; OTRPC1; VR-1; VR.5'sv; VR1; Vr1; Vr1l1; capsaicin receptor; vanilloid receptor subtype 1;
Molecular Note		The gene was disrupted by deleting an exon encoding part of the fifth and all of the sixth putative transmembrane domains of the channel, together with the intervening pore-loop region. Northern blot analysis showed that mRNA derived from dorsal root ganglia (DRG) of homozygous mice lacked detectable transcripts encoded by this allele. [MGI Ref ID J:61534]

Genotyping

Genotyping Information

Genotyping Protocols

Trpv1^tm1Jul, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Caterina MJ; Leffler A; Malmberg AB; Martin WJ; Trafton J; Petersen-Zeitz KR; Koltzenburg M; Basbaum AI; Julius D. 2000. Impaired nociception and pain sensation in mice lacking the capsaicin receptor [see comments] Science 288(5464):306-13. [PubMed: 10764638]  [MGI Ref ID J:61534]

Additional References

Alter BJ; Gereau RW th. 2008. Hotheaded: TRPV1 as Mediator of Hippocampal Synaptic Plasticity. Neuron 57(5):629-31. [PubMed: 18341983]  [MGI Ref ID J:131803]

Trpv1^tm1Jul related

Ahern GP; Brooks IM; Miyares RL; Wang XB. 2005. Extracellular cations sensitize and gate capsaicin receptor TRPV1 modulating pain signaling. J Neurosci 25(21):5109-16. [PubMed: 15917451]  [MGI Ref ID J:98540]

Akopian AN; Ruparel NB; Jeske NA; Hargreaves KM. 2007. Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization. J Physiol 583(Pt 1):175-93. [PubMed: 17584831]  [MGI Ref ID J:140830]

Alter BJ; Gereau RW th. 2008. Hotheaded: TRPV1 as Mediator of Hippocampal Synaptic Plasticity. Neuron 57(5):629-31. [PubMed: 18341983]  [MGI Ref ID J:131803]

Amadesi S; Nie J; Vergnolle N; Cottrell GS; Grady EF; Trevisani M; Manni C; Geppetti P; McRoberts JA; Ennes H; Davis JB; Mayer EA; Bunnett NW. 2004. Protease-activated receptor 2 sensitizes the capsaicin receptor transient receptor potential vanilloid receptor 1 to induce hyperalgesia. J Neurosci 24(18):4300-12. [PubMed: 15128844]  [MGI Ref ID J:96901]

Baiou D; Santha P; Avelino A; Charrua A; Bacskai T; Matesz K; Cruz F; Nagy I. 2007. Neurochemical characterization of insulin receptor-expressing primary sensory neurons in wild-type and vanilloid type 1 transient receptor potential receptor knockout mice. J Comp Neurol 503(2):334-47. [PubMed: 17492627]  [MGI Ref ID J:132840]

Bautista DM; Sigal YM; Milstein AD; Garrison JL; Zorn JA; Tsuruda PR; Nicoll RA; Julius D. 2008. Pungent agents from Szechuan peppers excite sensory neurons by inhibiting two-pore potassium channels. Nat Neurosci 11(7):772-9. [PubMed: 18568022]  [MGI Ref ID J:139352]

Bielefeldt K; Davis BM. 2008. Differential effects of ASIC3 and TRPV1 deletion on gastroesophageal sensation in mice. Am J Physiol Gastrointest Liver Physiol 294(1):G130-8. [PubMed: 17975130]  [MGI Ref ID J:130510]

Birder LA; Nakamura Y; Kiss S; Nealen ML; Barrick S; Kanai AJ; Wang E; Ruiz G; De Groat WC; Apodaca G; Watkins S; Caterina MJ. 2002. Altered urinary bladder function in mice lacking the vanilloid receptor TRPV1. Nat Neurosci 5(9):856-60. [PubMed: 12161756]  [MGI Ref ID J:78707]

Biro T; Bodo E; Telek A; Geczy T; Tychsen B; Kovacs L; Paus R. 2006. Hair cycle control by vanilloid receptor-1 (TRPV1): evidence from TRPV1 knockout mice. J Invest Dermatol 126(8):1909-12. [PubMed: 16645591]  [MGI Ref ID J:110694]

Cao X; Cao X; Xie H; Yang R; Lei G; Li F; Li A; Liu C; Liu L. 2007. Effects of capsaicin on VGSCs in TRPV1(-/-) mice. Brain Res 1163:33-43. [PubMed: 17632091]  [MGI Ref ID J:123769]

Christoph T; Bahrenberg G; De Vry J; Englberger W; Erdmann VA; Frech M; Kogel B; Rohl T; Schiene K; Schroder W; Seibler J; Kurreck J. 2008. Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice. Mol Cell Neurosci 37(3):579-89. [PubMed: 18249134]  [MGI Ref ID J:132700]

Ciura S; Bourque CW. 2006. Transient receptor potential vanilloid 1 is required for intrinsic osmoreception in organum vasculosum lamina terminalis neurons and for normal thirst responses to systemic hyperosmolality. J Neurosci 26(35):9069-75. [PubMed: 16943565]  [MGI Ref ID J:112194]

Clark N; Keeble J; Fernandes ES; Starr A; Liang L; Sugden D; de Winter P; Brain SD. 2007. The transient receptor potential vanilloid 1 (TRPV1) receptor protects against the onset of sepsis after endotoxin. FASEB J 21(13):3747-55. [PubMed: 17601984]  [MGI Ref ID J:134903]

Costa RM; Liu L; Nicolelis MA; Simon SA. 2005. Gustatory Effects of Capsaicin that are Independent of TRPV1 Receptors. Chem Senses 30 Suppl 1:i198-i200. [PubMed: 15738113]  [MGI Ref ID J:124409]

Dai Y; Moriyama T; Higashi T; Togashi K; Kobayashi K; Yamanaka H; Tominaga M; Noguchi K. 2004. Proteinase-activated receptor 2-mediated potentiation of transient receptor potential vanilloid subfamily 1 activity reveals a mechanism for proteinase-induced inflammatory pain. J Neurosci 24(18):4293-9. [PubMed: 15128843]  [MGI Ref ID J:96902]

Eckert WA 3rd; Julius D; Basbaum AI. 2006. Differential contribution of TRPV1 to thermal responses and tissue injury-induced sensitization of dorsal horn neurons in laminae I and V in the mouse. Pain 126(1-3):184-97. [PubMed: 16945484]  [MGI Ref ID J:117569]

Gibson HE; Edwards JG; Page RS; Van Hook MJ; Kauer JA. 2008. TRPV1 channels mediate long-term depression at synapses on hippocampal interneurons. Neuron 57(5):746-59. [PubMed: 18341994]  [MGI Ref ID J:132936]

Iida T; Shimizu I; Nealen ML; Campbell A; Caterina M. 2005. Attenuated fever response in mice lacking TRPV1. Neurosci Lett 378(1):28-33. [PubMed: 15763167]  [MGI Ref ID J:104828]

Jin K; Xie L; Kim SH; Parmentier-Batteur S; Sun Y; Mao XO; Childs J; Greenberg DA. 2004. Defective adult neurogenesis in CB1 cannabinoid receptor knockout mice. Mol Pharmacol 66(2):204-8. [PubMed: 15266010]  [MGI Ref ID J:102006]

Jones RC 3rd; Xu L; Gebhart GF. 2005. The mechanosensitivity of mouse colon afferent fibers and their sensitization by inflammatory mediators require transient receptor potential vanilloid 1 and acid-sensing ion channel 3. J Neurosci 25(47):10981-9. [PubMed: 16306411]  [MGI Ref ID J:103423]

Jones RC rd; Otsuka E; Wagstrom E; Jensen CS; Price MP; Gebhart GF. 2007. Short-term sensitization of colon mechanoreceptors is associated with long-term hypersensitivity to colon distention in the mouse. Gastroenterology 133(1):184-94. [PubMed: 17553498]  [MGI Ref ID J:128319]

Katanosaka K; Banik RK; Giron R; Higashi T; Tominaga M; Mizumura K. 2008. Contribution of TRPV1 to the bradykinin-evoked nociceptive behavior and excitation of cutaneous sensory neurons. Neurosci Res 62(3):168-75. [PubMed: 18789982]  [MGI Ref ID J:141239]

Kawamata T; Ji W; Yamamoto J; Niiyama Y; Furuse S; Namiki A. 2008. Contribution of transient receptor potential vanilloid subfamily 1 to endothelin-1-induced thermal hyperalgesia. Neuroscience 154(3):1067-76. [PubMed: 18495351]  [MGI Ref ID J:140794]

Keeble JE; Brain SD. 2006. Capsaicin-induced vasoconstriction in the mouse knee joint: a study using TRPV1 knockout mice. Neurosci Lett 401(1-2):55-8. [PubMed: 16584841]  [MGI Ref ID J:111113]

Koo JY; Jang Y; Cho H; Lee CH; Jang KH; Chang YH; Shin J; Oh U. 2007. Hydroxy-alpha-sanshool activates TRPV1 and TRPA1 in sensory neurons. Eur J Neurosci 26(5):1139-47. [PubMed: 17767493]  [MGI Ref ID J:127256]

Leffler A; Monter B; Koltzenburg M. 2006. The role of the capsaicin receptor TRPV1 and acid-sensing ion channels (ASICS) in proton sensitivity of subpopulations of primary nociceptive neurons in rats and mice. Neuroscience 139(2):699-709. [PubMed: 16515841]  [MGI Ref ID J:107758]

Lyall V; Heck GL; Phan TH; Mummalaneni S; Malik SA; Vinnikova AK; Desimone JA. 2005. Ethanol modulates the VR-1 variant amiloride-insensitive salt taste receptor. II. Effect on chorda tympani salt responses. J Gen Physiol 125(6):587-600. [PubMed: 15928404]  [MGI Ref ID J:112497]

Lyall V; Heck GL; Vinnikova AK; Ghosh S; Phan TH; Alam RI; Russell OF; Malik SA; Bigbee JW; DeSimone JA. 2004. The mammalian amiloride-insensitive non-specific salt taste receptor is a vanilloid receptor-1 variant. J Physiol 558(Pt 1):147-59. [PubMed: 15146042]  [MGI Ref ID J:105344]

Maccarrone M; Rossi S; Bari M; De Chiara V; Fezza F; Musella A; Gasperi V; Prosperetti C; Bernardi G; Finazzi-Agro A; Cravatt BF; Centonze D. 2008. Anandamide inhibits metabolism and physiological actions of 2-arachidonoylglycerol in the striatum. Nat Neurosci 11(2):152-9. [PubMed: 18204441]  [MGI Ref ID J:131396]

Marsch R; Foeller E; Rammes G; Bunck M; Kossl M; Holsboer F; Zieglgansberger W; Landgraf R; Lutz B; Wotjak CT. 2007. Reduced anxiety, conditioned fear, and hippocampal long-term potentiation in transient receptor potential vanilloid type 1 receptor-deficient mice. J Neurosci 27(4):832-9. [PubMed: 17251423]  [MGI Ref ID J:117778]

Matta JA; Cornett PM; Miyares RL; Abe K; Sahibzada N; Ahern GP. 2008. General anesthetics activate a nociceptive ion channel to enhance pain and inflammation. Proc Natl Acad Sci U S A 105(25):8784-9. [PubMed: 18574153]  [MGI Ref ID J:137166]

Moriyama T; Iida T; Kobayashi K; Higashi T; Fukuoka T; Tsumura H; Leon C; Suzuki N; Inoue K; Gachet C; Noguchi K; Tominaga M. 2003. Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity. J Neurosci 23(14):6058-62. [PubMed: 12853424]  [MGI Ref ID J:120670]

Motter AL; Ahern GP. 2008. TRPV1-null mice are protected from diet-induced obesity. FEBS Lett 582(15):2257-62. [PubMed: 18503767]  [MGI Ref ID J:137026]

Naeini RS; Witty MF; Seguela P; Bourque CW. 2006. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nat Neurosci 9(1):93-8. [PubMed: 16327782]  [MGI Ref ID J:105254]

Pacher P; Batkai S; Kunos G. 2004. Haemodynamic profile and responsiveness to anandamide of TRPV1 receptor knock-out mice. J Physiol 558(Pt 2):647-57. [PubMed: 15121805]  [MGI Ref ID J:105525]

Pogatzki-Zahn EM; Shimizu I; Caterina M; Raja SN. 2005. Heat hyperalgesia after incision requires TRPV1 and is distinct from pure inflammatory pain. Pain 115(3):296-307. [PubMed: 15911156]  [MGI Ref ID J:101955]

Salazar H; Llorente I; Jara-Oseguera A; Garcia-Villegas R; Munari M; Gordon SE; Islas LD; Rosenbaum T. 2008. A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. Nat Neurosci 11(3):255-61. [PubMed: 18297068]  [MGI Ref ID J:135695]

Shim WS; Tak MH; Lee MH; Kim M; Kim M; Koo JY; Lee CH; Kim M; Oh U. 2007. TRPV1 mediates histamine-induced itching via the activation of phospholipase A2 and 12-lipoxygenase. J Neurosci 27(9):2331-7. [PubMed: 17329430]  [MGI Ref ID J:119817]

Shimizu I; Iida T; Guan Y; Zhao C; Raja SN; Jarvis MF; Cockayne DA; Caterina MJ. 2005. Enhanced thermal avoidance in mice lacking the ATP receptor P2X3. Pain 116(1-2):96-108. [PubMed: 15927378]  [MGI Ref ID J:103789]

Sugiuar T; Bielefeldt K; Gebhart GF. 2004. TRPV1 function in mouse colon sensory neurons is enhanced by metabotropic 5-hydroxytryptamine receptor activation. J Neurosci 24(43):9521-30. [PubMed: 15509739]  [MGI Ref ID J:109767]

Symanowicz PT; Gianutsos G; Morris JB. 2004. Lack of role for the vanilloid receptor in response to several inspired irritant air pollutants in the C57Bl/6J mouse. Neurosci Lett 362(2):150-3. [PubMed: 15193774]  [MGI Ref ID J:121241]

Taylor AC; McCarthy JJ; Stocker SD. 2008. Mice lacking the transient receptor vanilloid potential 1 channel display normal thirst responses and central Fos activation to hypernatremia. Am J Physiol Regul Integr Comp Physiol 294(4):R1285-93. [PubMed: 18272658]  [MGI Ref ID J:133326]

Treesukosol Y; Lyall V; Heck GL; Desimone JA; Spector AC. 2007. A psychophysical and electrophysiological analysis of salt taste in Trpv1 null mice. Am J Physiol Regul Integr Comp Physiol 292(5):R1799-809. [PubMed: 17234959]  [MGI Ref ID J:121472]

Wang L; Wang DH. 2005. TRPV1 gene knockout impairs postischemic recovery in isolated perfused heart in mice. Circulation 112(23):3617-23. [PubMed: 16314376]  [MGI Ref ID J:116905]

Zhang LL; Yan Liu D; Ma LQ; Luo ZD; Cao TB; Zhong J; Yan ZC; Wang LJ; Zhao ZG; Zhu SJ; Schrader M; Thilo F; Zhu ZM; Tepel M. 2007. Activation of transient receptor potential vanilloid type-1 channel prevents adipogenesis and obesity. Circ Res 100(7):1063-70. [PubMed: 17347480]  [MGI Ref ID J:133914]

Zhong B; Wang DH. 2008. N-oleoyldopamine, a novel endogenous capsaicin-like lipid, protects the heart against ischemia-reperfusion injury via activation of TRPV1. Am J Physiol Heart Circ Physiol 295(2):H728-35. [PubMed: 18567714]  [MGI Ref ID J:138221]

Zhong B; Wang DH. 2007. TRPV1 gene knockout impairs preconditioning protection against myocardial injury in isolated perfused hearts in mice. Am J Physiol Heart Circ Physiol 293(3):H1791-8. [PubMed: 17586621]  [MGI Ref ID J:126121]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX8

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are bred as homozygotes. Coat color expected from breeding:Black
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

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The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.