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Strain Name:

B6.Cg-Tg(Nes-cre)1Kln/J

Stock Number:

003771

Availability:

Repository- Live


General Terms and Conditions

Former Name      B6.Cg(SJL)-Tg(Nes-cre)1Kln/J    (Changed: 15-DEC-04 )
      B6.Cg(SJL)-TgN(NesCre)1Kln    (Changed: 15-DEC-04 )
Genes & Alleles   Nes;   cre;   Tg(Nes-cre)1Kln;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Mating SystemHemizygote x +/+ sibling         (Female x Male)
Specieslaboratory mouse
Donating Investigator Rüdiger Klein,   Max-Planck Institute of Neurobiology
GenerationN6+4F13 (05-DEC-07)

Strain Description
These transgenic mice express Cre recombinase under the control of the rat nestin promoter and enhancer. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Initial studies utilizing a reporter strain carrying a beta galactosidase transgene whose expression is dependent on Cre-mediated recombination indicate that cre is primarily expressed in the central and peripheral nervous system with a few isolated kidney and heart cells also expressing activity. The donating investigator indicates that Cre recombinase activity is present in nervous tissue by embryonic day 11.

Strain Development
A transgenic construct containing a human growth hormone polyadenylation signal and a Cre recombinase gene under the control of the promoter and the nervous system-specific enhancer present in the second intron of the rat nestin gene, was injected into B6SJLF2 oocytes. Founder animals were bred to wildtype C57BL/6J mice.

Gene & Allele Details

Allele Symbol Tg(Nes-cre)1Kln
Allele Name transgene insertion 1, Rudiger Klein
Common Name(s) NesCre; Tg(Nes-cre); nestin-Cre; nestin/Cre;
Mutation Made By Rüdiger Klein,   Max-Planck Institute of Neurobiology
Strain of Origin(C57BL/6 x SJL)F2
Site of Expressionprimarily expressed in the central and peripheral nervous system by embryonic day 11 and a few isolated kidney and heart cells
Expressed Gene cre, cre recombinase, bacteriophage P1
Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence.
Promoter Nes, nestin, rat
General Note Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
Molecular Note This transgene expresses Cre recombinase under the control of a rat nestin (Nes) promoter. This promoter expresses Cre recombinase in neuronal and glial cell precursor. Cre recombinase activity is present in nervous tissue by embryonic day 11. [MGI Ref ID J:133340] [MGI Ref ID J:57315]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Genotyping Protocols

Generic Cre

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6SJLF2 background and has been backcrossed to C57BL/6J for at least six generations. Coat color expected from breeding:Black
Diet Information LabDiet® 5K52/5K67

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005697   B6.129-Otx1tm4(cre)Asim/J
004146   B6.129-Tg(Pcp2-cre)2Mpin/J
006785   B6.129P2(C)-Cd19tm1(cre)Cgn/J
006084   B6.129P2(Cg)-Foxg1tm1(cre)Skm/J
004781   B6.129P2-Lyz2tm1(cre)Ifo/J
005623   B6.129S-Shhtm2(cre/ESR1)Cjt/J
006600   B6.129S1-Mnx1tm4(cre)Tmj/J
005628   B6.129S2-Emx1tm1(cre)Krj/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
006878   B6.129S6-Taglntm2(cre)Yec/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
006230   B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
006149   B6.Cg-Tg(ACTA1-cre)79Jme/J
003574   B6.Cg-Tg(Alb-cre)21Mgn/J
006881   B6.Cg-Tg(Aqp2-cre)1Dek/J
004682   B6.Cg-Tg(CAG-cre/Esr1)5Amc/J
005359   B6.Cg-Tg(Camk2a-cre)T29-1Stl/J
006137   B6.Cg-Tg(Cdh5-cre)7Mlia/J
006368   B6.Cg-Tg(Cr2-cre)3Cgn/J
006663   B6.Cg-Tg(Eno2-cre)39Jme/J
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
003556   B6.Cg-Tg(Mx1-cre)1Cgn/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
005657   B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J
005975   B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J
005584   B6.Cg-Tg(Prrx1-cre)1Cjt/J
003967   B6.Cg-Tg(Rbp3-cre)528Jxm/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
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004128   B6.Cg-Tg(Tek-cre)12Flv/J
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006234   B6.Cg-Tg(tetO-cre)1Jaw/J
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006333   B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
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View Strains carrying other alleles of cre     (112 strains)

Additional Web Information

Congenic Nomenclature
Cre-lox or FLP-FRT Systems
JAX Notes, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)

Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Neurobiology Research

cre related

Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)

References

Selected Reference(s)

Tronche F; Kellendonk C; Kretz O; Gass P; Anlag K; Orban PC; Bock R; Klein R; Schutz G. 1999. Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety. Nat Genet 23(1):99-103. [PubMed: 10471508]  [MGI Ref ID J:57315]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(Nes-cre)1Kln/J
Stock Number: 003771

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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