Strain Name: |
B6.Cg-Tg(Nes-cre)1Kln/J |
|---|---|
Stock Number: |
003771 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg(SJL)-Tg(Nes-cre)1Kln/J (Changed: 15-DEC-04
) |
|
B6.Cg(SJL)-TgN(NesCre)1Kln (Changed: 15-DEC-04
) | |
| Genes & Alleles | Nes; cre; Tg(Nes-cre)1Kln; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Rüdiger Klein, Max-Planck Institute of Neurobiology Generation N6+4F13 (05-DEC-07) Strain Description
These transgenic mice express Cre recombinase under the control of the rat nestin promoter and enhancer. Mice that are hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Initial studies utilizing a reporter strain carrying a beta galactosidase transgene whose expression is dependent on Cre-mediated recombination indicate that cre is primarily expressed in the central and peripheral nervous system with a few isolated kidney and heart cells also expressing activity. The donating investigator indicates that Cre recombinase activity is present in nervous tissue by embryonic day 11.Strain Development
A transgenic construct containing a human growth hormone polyadenylation signal and a Cre recombinase gene under the control of the promoter and the nervous system-specific enhancer present in the second intron of the rat nestin gene, was injected into B6SJLF2 oocytes. Founder animals were bred to wildtype C57BL/6J mice.
Gene & Allele Details
| Allele Symbol | Tg(Nes-cre)1Kln | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Rudiger Klein | ||
| Common Name(s) | NesCre; Tg(Nes-cre); nestin-Cre; nestin/Cre; | ||
| Mutation Made By | Rüdiger Klein, Max-Planck Institute of Neurobiology | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | primarily expressed in the central and peripheral nervous system by embryonic day 11 and a few isolated kidney and heart cells | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Nes, nestin, rat | ||
| General Note | Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a rat nestin (Nes) promoter. This promoter expresses Cre recombinase in neuronal and glial cell precursor. Cre recombinase activity is present in nervous tissue by embryonic day 11. [MGI Ref ID J:133340] [MGI Ref ID J:57315] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | This strain originated on a B6SJLF2 background and has been backcrossed to C57BL/6J for at least six generations. Coat color expected from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (112 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
JAX Notes, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesTronche F; Kellendonk C; Kretz O; Gass P; Anlag K; Orban PC; Bock R; Klein R; Schutz G. 1999. Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety. Nat Genet 23(1):99-103. [PubMed: 10471508] [MGI Ref ID J:57315]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Nes-cre)1Kln/J |
| Stock Number: | 003771 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form