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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Mating System Inbred x Hemizygote (Female x Male) Species laboratory mouse Generation N5+N17 (06-DEC-07) Donating Investigator Joshua Sanes, Harvard University Description
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of strain 003709. The primary difference between these two strains is the specific neuron subsets which express YFP. In this strain, a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescence appears somewhat dim at birth but brightens in adult mice. Availability of multiple spectral variants is useful for double-labeling applications or breeding to other CFP/GFP lines.Development
A transgenic construct containing a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (173 strains)
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-YFPH)2Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Joshua R Sanes | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | YFP-H; thy1-YFP line H; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | Sensory neurons in dorsal root ganglia, and pyramidal cells in Layer 5 of the cerebral cortex and in the hippocampus express YFP, 10-30% of retinal ganglion cells are exclusively labeled in the retina, mossy fibers are strongly labeled in cerebellar cortex. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| General Note | Transgenic mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells.In these mice a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescenceappears somewhat dim at birth but brightens in adult mice. | ||
| Molecular Note | The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. The regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4, but lacking exon 3 and its flanking introns. The same construct was used to generate Tg(Thy1-YFP)16Jrs. [MGI Ref ID J:93699] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-YFP)16Jrs, Tg(Thy1-YFPH)2Jrs, HRM, vers. 2
Tg(Thy1-YFP)16Jrs, Tg(Thy1-YFPH)2Jrs, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Additional References
Dombeck DA; Khabbaz AN; Collman F; Adelman TL; Tank DW. 2007. Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile Mice. Neuron 56(1):43-57. [PubMed: 17920014] [MGI Ref ID J:125132]
Misgeld T; Nikic I; Kerschensteiner M. 2007. In vivo imaging of single axons in the mouse spinal cord. Nat Protoc 2(2):263-8. [PubMed: 17406584] [MGI Ref ID J:122714]
Niu S; Renfro A; Quattrocchi CC; Sheldon M; D'Arcangelo G. 2004. Reelin Promotes Hippocampal Dendrite Development through the VLDLR/ApoER2-Dab1 Pathway. Neuron 41(1):71-84. [PubMed: 14715136] [MGI Ref ID J:87427]
Tg(Thy1-YFPH)2Jrs relatedAngata K; Huckaby V; Ranscht B; Terskikh A; Marth JD; Fukuda M. 2007. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol 27(19):6659-68. [PubMed: 17682066] [MGI Ref ID J:126687]
Bannerman PG; Hahn A. 2007. Enhanced visualization of axonopathy in EAE using thy1-YFP transgenic mice. J Neurol Sci 260(1-2):23-32. [PubMed: 17493638] [MGI Ref ID J:136606]
Beirowski B; Adalbert R; Wagner D; Grumme DS; Addicks K; Ribchester RR; Coleman MP. 2005. The progressive nature of Wallerian degeneration in wild-type and slow Wallerian degeneration (WldS) nerves. BMC Neurosci 6(1):6. [PubMed: 15686598] [MGI Ref ID J:96660]
Conforti L; Fang G; Beirowski B; Wang MS; Sorci L; Asress S; Adalbert R; Silva A; Bridge K; Huang XP; Magni G; Glass JD; Coleman MP. 2007. NAD(+) and axon degeneration revisited: Nmnat1 cannot substitute for Wld(S) to delay Wallerian degeneration. Cell Death Differ 14(1):116-27. [PubMed: 16645633] [MGI Ref ID J:132236]
Demyanenko GP; Schachner M; Anton E; Schmid R; Feng G; Sanes J; Maness PF. 2004. Close homolog of L1 modulates area-specific neuronal positioning and dendrite orientation in the cerebral cortex. Neuron 44(3):423-37. [PubMed: 15504324] [MGI Ref ID J:96525]
Dombeck DA; Khabbaz AN; Collman F; Adelman TL; Tank DW. 2007. Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile Mice. Neuron 56(1):43-57. [PubMed: 17920014] [MGI Ref ID J:125132]
English AW; Meador W; Carrasco DI. 2005. Neurotrophin-4/5 is required for the early growth of regenerating axons in peripheral nerves. Eur J Neurosci 21(10):2624-34. [PubMed: 15926911] [MGI Ref ID J:101068]
Feng Y; Walsh CA. 2004. Mitotic spindle regulation by Nde1 controls cerebral cortical size. Neuron 44(2):279-93. [PubMed: 15473967] [MGI Ref ID J:94756]
Gastinger MJ; Kunselman AR; Conboy EE; Bronson SK; Barber AJ. 2008. Dendrite remodeling and other abnormalities in the retinal ganglion cells of Ins2 Akita diabetic mice. Invest Ophthalmol Vis Sci 49(6):2635-42. [PubMed: 18515593] [MGI Ref ID J:137045]
Groves ML; McKeon R; Werner E; Nagarsheth M; Meador W; English AW. 2005. Axon regeneration in peripheral nerves is enhanced by proteoglycan degradation. Exp Neurol 195(2):278-92. [PubMed: 15950970] [MGI Ref ID J:131689]
Gu C; Rodriguez ER; Reimert DV; Shu T; Fritzsch B; Richards LJ; Kolodkin AL; Ginty DD. 2003. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev Cell 5(1):45-57. [PubMed: 12852851] [MGI Ref ID J:93799]
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Kim JV; Dustin ML. 2006. Innate response to focal necrotic injury inside the blood-brain barrier. J Immunol 177(8):5269-77. [PubMed: 17015712] [MGI Ref ID J:139445]
Li X; Zhang P; Yang Y; Xiong Y; Qi Y; Hu H. 2008. Differentiation and developmental origin of cerebellar granule neuron ectopia in protein O-mannose UDP-N-acetylglucosaminyl transferase 1 knockout mice. Neuroscience 152(2):391-406. [PubMed: 18295407] [MGI Ref ID J:135670]
Liu X; Grishanin RN; Tolwani RJ; Renteria RC; Xu B; Reichardt LF; Copenhagen DR. 2007. Brain-derived neurotrophic factor and TrkB modulate visual experience-dependent refinement of neuronal pathways in retina. J Neurosci 27(27):7256-67. [PubMed: 17611278] [MGI Ref ID J:122929]
Lorenzetto E; Panteri R; Marino R; Keller F; Buffelli M. 2008. Impaired nerve regeneration in reeler mice after peripheral nerve injury. Eur J Neurosci 27(1):12-9. [PubMed: 18093172] [MGI Ref ID J:132197]
Niu S; Yabut O; D'Arcangelo G. 2008. The Reelin signaling pathway promotes dendritic spine development in hippocampal neurons. J Neurosci 28(41):10339-48. [PubMed: 18842893] [MGI Ref ID J:141125]
Probst B; Rock R; Gessler M; Vortkamp A; Puschel AW. 2007. The rodent Four-jointed ortholog Fjx1 regulates dendrite extension. Dev Biol 312(1):461-70. [PubMed: 18028897] [MGI Ref ID J:130206]
Schaefer AM; Sanes JR; Lichtman JW. 2005. A compensatory subpopulation of motor neurons in a mouse model of amyotrophic lateral sclerosis. J Comp Neurol 490(3):209-19. [PubMed: 16082680] [MGI Ref ID J:102773]
Stanwood GD; Parlaman JP; Levitt P. 2005. Anatomical abnormalities in dopaminoceptive regions of the cerebral cortex of dopamine D1 receptor mutant mice. J Comp Neurol 487(3):270-82. [PubMed: 15892099] [MGI Ref ID J:99709]
Tsai J; Grutzendler J; Duff K; Gan WB. 2004. Fibrillar amyloid deposition leads to local synaptic abnormalities and breakage of neuronal branches. Nat Neurosci 7(11):1181-3. [PubMed: 15475950] [MGI Ref ID J:94364]
Zhang S; Boyd J; Delaney K; Murphy TH. 2005. Rapid reversible changes in dendritic spine structure in vivo gated by the degree of ischemia. J Neurosci 25(22):5333-8. [PubMed: 15930381] [MGI Ref ID J:134932]
Zuo Y; Lin A; Chang P; Gan WB. 2005. Development of long-term dendritic spine stability in diverse regions of cerebral cortex. Neuron 46(2):181-9. [PubMed: 15848798] [MGI Ref ID J:130614]
Zuo Y; Yang G; Kwon E; Gan WB. 2005. Long-term sensory deprivation prevents dendritic spine loss in primary somatosensory cortex. Nature 436(7048):261-5. [PubMed: 16015331] [MGI Ref ID J:131883]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Inbred x Hemizygote (Female x Male) Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $232.00 Female or Male Hemizygous for Tg(Thy1-YFPH)2Jrs *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $248.90 C57BL/6J (000664) x Hemizygous for Tg(Thy1-YFPH)2Jrs $247.50 Hemizygous for Tg(Thy1-YFPH)2Jrs x C57BL/6J (000664)
Additional Supply Details
| Supply Notes |
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| Pricing for International shipping destinations |
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Weeks of Age Price* Gender Genotypes Provided Individual Mouse Price $301.60 Female or Male Hemizygous for Tg(Thy1-YFPH)2Jrs *Price(s) in US dollars ($)
Pairs /Price* Pair Genotype $323.60 C57BL/6J (000664) x Hemizygous for Tg(Thy1-YFPH)2Jrs $321.80 Hemizygous for Tg(Thy1-YFPH)2Jrs x C57BL/6J (000664)
Additional Supply Details
| Supply Notes |
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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