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| These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. This line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. | |||||||||||||||||||
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Description
Strain Information
Former Names B6.Cg-Tg(Thy1-YFPH)2Jrs/J (Changed: 01-NOV-11 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N5+N20 (01-JAN-09)
Generation DefinitionsDonating Investigator Joshua R Sanes, Harvard University Description
These mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells. The transgenic insert used to make this strain is identical to that used in the construction of strain 003709. The primary difference between these two strains is the specific neuron subsets which express YFP. In this strain, a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescence appears somewhat dim at birth but brightens in adult mice. Availability of multiple spectral variants is useful for double-labeling applications or breeding to other CFP/GFP lines.Development
A transgenic construct containing a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene was injected into fertilized B6CBAF1 mouse eggs. Regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4. Exon 3 and its flanking introns are absent. The deleted sequences are required for expression in non-neural cells but not in neurons. The remainder of the sequence is required for neuronal expression. Founder animals were obtained and crossed with wildtype C57BL/6J mice.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
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Additional Web Information
Fluorescent Proteins/lacZ Systems
JAX® NOTES, Summer 2002; 486. Visualizing JAX® Mice Strains Carrying Fluorescent Proteins.
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-YFP)HJrs | ||
|---|---|---|---|
| Allele Name | transgene insertion H, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Tg(Thy1-YFPH)2Jrs; TgN(Thy1-YFP-H)2Jrs; YFP-H; thy1-YFP line H; thy1-YFP-H; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6J x CBA)F1 | ||
| Site of Expression | Sensory neurons in dorsal root ganglia, and pyramidal cells in Layer 5 of the cerebral cortex and in the hippocampus express YFP, 10-30% of retinal ganglion cells are exclusively labeled in the retina, mossy fibers are strongly labeled in cerebellar cortex. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Thy1-YFP)HJrs, transgene insertion H, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(Thy1-YFPH)2Jrs; YFP-H; thy1-YFP line H; thy1-YFP-16Jrs; | ||
| General Note | Transgenic mice express spectral variants of GFP (yellow-YFP) at high levels in motor and sensory neurons, as well as subsets of central neurons. Axons are brightly fluorescent all the way to the terminals. No expression is detectable in nonneural cells.In these mice a few motor axons are labeled in muscle tissue, allowing determination of branching pattern and definition of which muscle fibers are innervated by a single motor axon. Approximately 10-30% of sensory neurons are labeled in dorsal root ganglia. Layer 5 pyramidal cells are selectively labeled in cerebral cortex. Pyramidal neurons are selectively labeled in the hippocampus. Approximately 10-30% of retinal ganglion cells are exclusively labeled in the retina. Many (but not all) mossy fibers are strongly labeled in cerebellar cortex, but no neurons are labeled in this area. In short, this line provides a strong and specific "Golgi-like" vital marker for several widely studied neuronal subsets, with minimal labeling of nearby axons. Fluorescenceappears somewhat dim at birth but brightens in adult mice. | ||
| Molecular Note | The transgene contains a YFP gene under the direction of regulatory elements derived from the mouse Thy1 gene. The regulatory elements are composed of a 6.5 kb fragment obtained from the 5' portion of the Thy1 gene, extending from the promoter to the intron following exon 4, but lacking exon 3 and its flanking introns. Nine lines were generated (12, 16, 21, A, C, D, F, G, and H). [MGI Ref ID J:93699] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Thy1-YFP)HJrs-alternate2,MELT
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Feng G; Mellor RH; Bernstein M; Keller-Peck C; Nguyen QT; Wallace M; Nerbonne JM; Lichtman JW; Sanes JR. 2000. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron 28(1):41-51. [PubMed: 11086982] [MGI Ref ID J:93699]
Porrero C; Rubio-Garrido P; Avendano C; Clasca F. 2010. Mapping of fluorescent protein-expressing neurons and axon pathways in adult and developing Thy1-eYFP-H transgenic mice. Brain Res 1345:59-72. [PubMed: 20510892] [MGI Ref ID J:161506]
Additional References
Dombeck DA; Khabbaz AN; Collman F; Adelman TL; Tank DW. 2007. Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile Mice. Neuron 56(1):43-57. [PubMed: 17920014] [MGI Ref ID J:125132]
Misgeld T; Nikic I; Kerschensteiner M. 2007. In vivo imaging of single axons in the mouse spinal cord. Nat Protoc 2(2):263-8. [PubMed: 17406584] [MGI Ref ID J:122714]
Niu S; Renfro A; Quattrocchi CC; Sheldon M; D'Arcangelo G. 2004. Reelin Promotes Hippocampal Dendrite Development through the VLDLR/ApoER2-Dab1 Pathway. Neuron 41(1):71-84. [PubMed: 14715136] [MGI Ref ID J:87427]
Tg(Thy1-YFP)HJrs relatedAngata K; Huckaby V; Ranscht B; Terskikh A; Marth JD; Fukuda M. 2007. Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development. Mol Cell Biol 27(19):6659-68. [PubMed: 17682066] [MGI Ref ID J:126687]
Babetto E; Beirowski B; Janeckova L; Brown R; Gilley J; Thomson D; Ribchester RR; Coleman MP. 2010. Targeting NMNAT1 to axons and synapses transforms its neuroprotective potency in vivo. J Neurosci 30(40):13291-304. [PubMed: 20926655] [MGI Ref ID J:165098]
Bannerman PG; Hahn A. 2007. Enhanced visualization of axonopathy in EAE using thy1-YFP transgenic mice. J Neurol Sci 260(1-2):23-32. [PubMed: 17493638] [MGI Ref ID J:136606]
Beaudoin GM 3rd; Schofield CM; Nuwal T; Zang K; Ullian EM; Huang B; Reichardt LF. 2012. Afadin, a ras/rap effector that controls cadherin function, promotes spine and excitatory synapse density in the hippocampus. J Neurosci 32(1):99-110. [PubMed: 22219273] [MGI Ref ID J:179366]
Beirowski B; Adalbert R; Wagner D; Grumme DS; Addicks K; Ribchester RR; Coleman MP. 2005. The progressive nature of Wallerian degeneration in wild-type and slow Wallerian degeneration (WldS) nerves. BMC Neurosci 6(1):6. [PubMed: 15686598] [MGI Ref ID J:96660]
Bittner T; Fuhrmann M; Burgold S; Jung CK; Volbracht C; Steiner H; Mitteregger G; Kretzschmar HA; Haass C; Herms J. 2009. gamma-Secretase inhibition reduces spine density in vivo via an amyloid precursor protein-dependent pathway. J Neurosci 29(33):10405-9. [PubMed: 19692615] [MGI Ref ID J:151919]
Bittner T; Fuhrmann M; Burgold S; Ochs SM; Hoffmann N; Mitteregger G; Kretzschmar H; LaFerla FM; Herms J. 2010. Multiple events lead to dendritic spine loss in triple transgenic Alzheimer's disease mice. PLoS One 5(11):e15477. [PubMed: 21103384] [MGI Ref ID J:166981]
Bogdanik LP; Burgess RW. 2011. A valid mouse model of AGRIN-associated congenital myasthenic syndrome. Hum Mol Genet :. [PubMed: 21890498] [MGI Ref ID J:176117]
Bridge KE; Berg N; Adalbert R; Babetto E; Dias T; Spillantini MG; Ribchester RR; Coleman MP. 2009. Late onset distal axonal swelling in YFP-H transgenic mice. Neurobiol Aging 30(2):309-21. [PubMed: 17658198] [MGI Ref ID J:145817]
Capetillo-Zarate E; Gracia L; Yu F; Banfelder JR; Lin MT; Tampellini D; Gouras GK. 2011. High-Resolution 3D Reconstruction Reveals Intra-Synaptic Amyloid Fibrils. Am J Pathol 179(5):2551-8. [PubMed: 21925470] [MGI Ref ID J:177369]
Charrier C; Joshi K; Coutinho-Budd J; Kim JE; Lambert N; de Marchena J; Jin WL; Vanderhaeghen P; Ghosh A; Sassa T; Polleux F. 2012. Inhibition of SRGAP2 function by its human-specific paralogs induces neoteny during spine maturation. Cell 149(4):923-35. [PubMed: 22559944] [MGI Ref ID J:185134]
Chen AI; Nguyen CN; Copenhagen DR; Badurek S; Minichiello L; Ranscht B; Reichardt LF. 2011. TrkB (Tropomyosin-Related Kinase B) Controls the Assembly and Maintenance of GABAergic Synapses in the Cerebellar Cortex. J Neurosci 31(8):2769-80. [PubMed: 21414899] [MGI Ref ID J:169866]
Chen Y; Hancock ML; Role LW; Talmage DA. 2010. Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons. J Neurosci 30(27):9199-208. [PubMed: 20610754] [MGI Ref ID J:161775]
Comley LH; Wishart TM; Baxter B; Murray LM; Nimmo A; Thomson D; Parson SH; Gillingwater TH. 2011. Induction of cell stress in neurons from transgenic mice expressing yellow fluorescent protein: implications for neurodegeneration research. PLoS One 6(3):e17639. [PubMed: 21408118] [MGI Ref ID J:171713]
Conforti L; Fang G; Beirowski B; Wang MS; Sorci L; Asress S; Adalbert R; Silva A; Bridge K; Huang XP; Magni G; Glass JD; Coleman MP. 2007. NAD(+) and axon degeneration revisited: Nmnat1 cannot substitute for Wld(S) to delay Wallerian degeneration. Cell Death Differ 14(1):116-27. [PubMed: 16645633] [MGI Ref ID J:132236]
Conforti L; Wilbrey A; Morreale G; Janeckova L; Beirowski B; Adalbert R; Mazzola F; Di Stefano M; Hartley R; Babetto E; Smith T; Gilley J; Billington RA; Genazzani AA; Ribchester RR; Magni G; Coleman M. 2009. Wld S protein requires Nmnat activity and a short N-terminal sequence to protect axons in mice. J Cell Biol 184(4):491-500. [PubMed: 19237596] [MGI Ref ID J:163222]
Deans MR; Krol A; Abraira VE; Copley CO; Tucker AF; Goodrich LV. 2011. Control of neuronal morphology by the atypical cadherin Fat3. Neuron 71(5):820-32. [PubMed: 21903076] [MGI Ref ID J:176668]
Demyanenko GP; Schachner M; Anton E; Schmid R; Feng G; Sanes J; Maness PF. 2004. Close homolog of L1 modulates area-specific neuronal positioning and dendrite orientation in the cerebral cortex. Neuron 44(3):423-37. [PubMed: 15504324] [MGI Ref ID J:96525]
Demyanenko GP; Siesser PF; Wright AG; Brennaman LH; Bartsch U; Schachner M; Maness PF. 2011. L1 and CHL1 Cooperate in Thalamocortical Axon Targeting. Cereb Cortex 21(2):401-12. [PubMed: 20576928] [MGI Ref ID J:171029]
Dombeck DA; Khabbaz AN; Collman F; Adelman TL; Tank DW. 2007. Imaging Large-Scale Neural Activity with Cellular Resolution in Awake, Mobile Mice. Neuron 56(1):43-57. [PubMed: 17920014] [MGI Ref ID J:125132]
English AW; Cucoranu D; Mulligan A; Rodriguez JA; Sabatier MJ. 2011. Neurotrophin-4/5 is implicated in the enhancement of axon regeneration produced by treadmill training following peripheral nerve injury. Eur J Neurosci 33(12):2265-71. [PubMed: 21623957] [MGI Ref ID J:176369]
English AW; Meador W; Carrasco DI. 2005. Neurotrophin-4/5 is required for the early growth of regenerating axons in peripheral nerves. Eur J Neurosci 21(10):2624-34. [PubMed: 15926911] [MGI Ref ID J:101068]
Farah MH; Pan BH; Hoffman PN; Ferraris D; Tsukamoto T; Nguyen T; Wong PC; Price DL; Slusher BS; Griffin JW. 2011. Reduced BACE1 Activity Enhances Clearance of Myelin Debris and Regeneration of Axons in the Injured Peripheral Nervous System. J Neurosci 31(15):5744-54. [PubMed: 21490216] [MGI Ref ID J:170970]
Feng Y; Walsh CA. 2004. Mitotic spindle regulation by Nde1 controls cerebral cortical size. Neuron 44(2):279-93. [PubMed: 15473967] [MGI Ref ID J:94756]
Fu M; Yu X; Lu J; Zuo Y. 2012. Repetitive motor learning induces coordinated formation of clustered dendritic spines in vivo. Nature 483(7387):92-5. [PubMed: 22343892] [MGI Ref ID J:181541]
Fuhrmann M; Bittner T; Jung CK; Burgold S; Page RM; Mitteregger G; Haass C; LaFerla FM; Kretzschmar H; Herms J. 2010. Microglial Cx3cr1 knockout prevents neuron loss in a mouse model of Alzheimer's disease. Nat Neurosci 13(4):411-3. [PubMed: 20305648] [MGI Ref ID J:159680]
Garrett AM; Schreiner D; Lobas MA; Weiner JA. 2012. gamma-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. Neuron 74(2):269-76. [PubMed: 22542181] [MGI Ref ID J:188387]
Gasparini L; Crowther RA; Martin KR; Berg N; Coleman M; Goedert M; Spillantini MG. 2011. Tau inclusions in retinal ganglion cells of human P301S tau transgenic mice: effects on axonal viability. Neurobiol Aging 32(3):419-33. [PubMed: 19356824] [MGI Ref ID J:173742]
Gastinger MJ; Kunselman AR; Conboy EE; Bronson SK; Barber AJ. 2008. Dendrite remodeling and other abnormalities in the retinal ganglion cells of Ins2 Akita diabetic mice. Invest Ophthalmol Vis Sci 49(6):2635-42. [PubMed: 18515593] [MGI Ref ID J:137045]
Gomez-Climent MA; Guirado R; Castillo-Gomez E; Varea E; Gutierrez-Mecinas M; Gilabert-Juan J; Garcia-Mompo C; Vidueira S; Sanchez-Mataredona D; Hernandez S; Blasco-Ibanez JM; Crespo C; Rutishauser U; Schachner M; Nacher J. 2011. The polysialylated form of the neural cell adhesion molecule (PSA-NCAM) is expressed in a subpopulation of mature cortical interneurons characterized by reduced structural features and connectivity. Cereb Cortex 21(5):1028-41. [PubMed: 20843898] [MGI Ref ID J:183825]
Goto K; Kato G; Kawahara I; Luo Y; Obata K; Misawa H; Ishikawa T; Kuniyasu H; Nabekura J; Takaki M. 2013. In vivo imaging of enteric neurogenesis in the deep tissue of mouse small intestine. PLoS One 8(1):e54814. [PubMed: 23382976] [MGI Ref ID J:195788]
Gould TW; Buss RR; Vinsant S; Prevette D; Sun W; Knudson CM; Milligan CE; Oppenheim RW. 2006. Complete dissociation of motor neuron death from motor dysfunction by Bax deletion in a mouse model of ALS. J Neurosci 26(34):8774-86. [PubMed: 16928866] [MGI Ref ID J:111890]
Groves ML; McKeon R; Werner E; Nagarsheth M; Meador W; English AW. 2005. Axon regeneration in peripheral nerves is enhanced by proteoglycan degradation. Exp Neurol 195(2):278-92. [PubMed: 15950970] [MGI Ref ID J:131689]
Gunnersen JM; Kuek A; Phipps JA; Hammond VE; Puthussery T; Fletcher EL; Tan SS. 2009. Seizure-related gene 6 (Sez-6) in amacrine cells of the rodent retina and the consequence of gene deletion. PLoS One 4(8):e6546. [PubMed: 19662096] [MGI Ref ID J:152476]
Ichinohe N; Knight A; Ogawa M; Ohshima T; Mikoshiba K; Yoshihara Y; Terashima T; Rockland KS. 2008. Unusual patch-matrix organization in the retrosplenial cortex of the reeler mouse and Shaking rat Kawasaki. Cereb Cortex 18(5):1125-38. [PubMed: 17728262] [MGI Ref ID J:158489]
Jaworski T; Lechat B; Demedts D; Gielis L; Devijver H; Borghgraef P; Duimel H; Verheyen F; Kugler S; Van Leuven F. 2011. Dendritic degeneration, neurovascular defects, and inflammation precede neuronal loss in a mouse model for tau-mediated neurodegeneration. Am J Pathol 179(4):2001-15. [PubMed: 21839061] [MGI Ref ID J:176300]
Joyal JS; Sitaras N; Binet F; Rivera JC; Stahl A; Zaniolo K; Shao Z; Polosa A; Zhu T; Hamel D; Djavari M; Kunik D; Honore JC; Picard E; Zabeida A; Varma DR; Hickson G; Mancini J; Klagsbrun M; Costantino S; Beausejour C; Lachapelle P; Smith LE; Chemtob S;Sapieha P. 2011. Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A. Blood 117(22):6024-35. [PubMed: 21355092] [MGI Ref ID J:177800]
Keller-Peck CR; Walsh MK; Gan WB; Feng G; Sanes JR; Lichtman JW. 2001. Asynchronous synapse elimination in neonatal motor units: studies using GFP transgenic mice. Neuron 31(3):381-94. [PubMed: 11516396] [MGI Ref ID J:71121]
Kim JV; Dustin ML. 2006. Innate response to focal necrotic injury inside the blood-brain barrier. J Immunol 177(8):5269-77. [PubMed: 17015712] [MGI Ref ID J:139445]
Lai CS; Franke TF; Gan WB. 2012. Opposite effects of fear conditioning and extinction on dendritic spine remodelling. Nature 483(7387):87-91. [PubMed: 22343895] [MGI Ref ID J:181540]
Li X; Zhang P; Yang Y; Xiong Y; Qi Y; Hu H. 2008. Differentiation and developmental origin of cerebellar granule neuron ectopia in protein O-mannose UDP-N-acetylglucosaminyl transferase 1 knockout mice. Neuroscience 152(2):391-406. [PubMed: 18295407] [MGI Ref ID J:135670]
Liu X; Grishanin RN; Tolwani RJ; Renteria RC; Xu B; Reichardt LF; Copenhagen DR. 2007. Brain-derived neurotrophic factor and TrkB modulate visual experience-dependent refinement of neuronal pathways in retina. J Neurosci 27(27):7256-67. [PubMed: 17611278] [MGI Ref ID J:122929]
Lorenzetto E; Panteri R; Marino R; Keller F; Buffelli M. 2008. Impaired nerve regeneration in reeler mice after peripheral nerve injury. Eur J Neurosci 27(1):12-9. [PubMed: 18093172] [MGI Ref ID J:132197]
Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40. [PubMed: 20023653] [MGI Ref ID J:155793]
Margolis DJ; Gartland AJ; Euler T; Detwiler PB. 2010. Dendritic calcium signaling in ON and OFF mouse retinal ganglion cells. J Neurosci 30(21):7127-38. [PubMed: 20505081] [MGI Ref ID J:165048]
Micheva KD; Busse B; Weiler NC; O'Rourke N; Smith SJ. 2010. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron 68(4):639-53. [PubMed: 21092855] [MGI Ref ID J:167857]
Nadeau S; Filali M; Zhang J; Kerr BJ; Rivest S; Soulet D; Iwakura Y; de Rivero Vaccari JP; Keane RW; Lacroix S. 2011. Functional Recovery after Peripheral Nerve Injury is Dependent on the Pro-Inflammatory Cytokines IL-1{beta} and TNF: Implications for Neuropathic Pain. J Neurosci 31(35):12533-12542. [PubMed: 21880915] [MGI Ref ID J:176222]
Niu S; Yabut O; D'Arcangelo G. 2008. The Reelin signaling pathway promotes dendritic spine development in hippocampal neurons. J Neurosci 28(41):10339-48. [PubMed: 18842893] [MGI Ref ID J:141125]
Ohshima T; Hirasawa M; Tabata H; Mutoh T; Adachi T; Suzuki H; Saruta K; Iwasato T; Itohara S; Hashimoto M; Nakajima K; Ogawa M; Kulkarni AB; Mikoshiba K. 2007. Cdk5 is required for multipolar-to-bipolar transition during radial neuronal migration and proper dendrite development of pyramidal neurons in the cerebral cortex. Development 134(12):2273-82. [PubMed: 17507397] [MGI Ref ID J:135129]
Ozdinler PH; Benn S; Yamamoto TH; Guzel M; Brown RH Jr; Macklis JD. 2011. Corticospinal Motor Neurons and Related Subcerebral Projection Neurons Undergo Early and Specific Neurodegeneration in hSOD1G93A Transgenic ALS Mice. J Neurosci 31(11):4166-4177. [PubMed: 21411657] [MGI Ref ID J:170453]
Pan F; Aldridge GM; Greenough WT; Gan WB. 2010. Dendritic spine instability and insensitivity to modulation by sensory experience in a mouse model of fragile X syndrome. Proc Natl Acad Sci U S A 107(41):17768-73. [PubMed: 20861447] [MGI Ref ID J:165411]
Pitt D; Gonzales E; Cross AH; Goldberg MP. 2010. Dysmyelinated axons in shiverer mice are highly vulnerable to alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor-mediated toxicity. Brain Res 1309:146-54. [PubMed: 19896473] [MGI Ref ID J:158545]
Pramparo T; Youn YH; Yingling J; Hirotsune S; Wynshaw-Boris A. 2010. Novel embryonic neuronal migration and proliferation defects in Dcx mutant mice are exacerbated by Lis1 reduction. J Neurosci 30(8):3002-12. [PubMed: 20181597] [MGI Ref ID J:157825]
Probst B; Rock R; Gessler M; Vortkamp A; Puschel AW. 2007. The rodent Four-jointed ortholog Fjx1 regulates dendrite extension. Dev Biol 312(1):461-70. [PubMed: 18028897] [MGI Ref ID J:130206]
Risher WC; Andrew RD; Kirov SA. 2009. Real-time passive volume responses of astrocytes to acute osmotic and ischemic stress in cortical slices and in vivo revealed by two-photon microscopy. Glia 57(2):207-21. [PubMed: 18720409] [MGI Ref ID J:156224]
Rupp NJ; Wegenast-Braun BM; Radde R; Calhoun ME; Jucker M. 2011. Early onset amyloid lesions lead to severe neuritic abnormalities and local, but not global neuron loss in APPPS1 transgenic mice. Neurobiol Aging 32(12):2324.e1-6. [PubMed: 20970889] [MGI Ref ID J:188225]
Schaefer AM; Sanes JR; Lichtman JW. 2005. A compensatory subpopulation of motor neurons in a mouse model of amyotrophic lateral sclerosis. J Comp Neurol 490(3):209-19. [PubMed: 16082680] [MGI Ref ID J:102773]
Schlomann U; Schwamborn JC; Muller M; Fassler R; Puschel AW. 2009. The stimulation of dendrite growth by Sema3A requires integrin engagement and focal adhesion kinase. J Cell Sci 122(Pt 12):2034-42. [PubMed: 19454481] [MGI Ref ID J:150568]
Schmidt H; Stonkute A; Juttner R; Koesling D; Friebe A; Rathjen FG. 2009. C-type natriuretic peptide (CNP) is a bifurcation factor for sensory neurons. Proc Natl Acad Sci U S A 106(39):16847-52. [PubMed: 19805384] [MGI Ref ID J:153212]
Schon C; Hoffmann NA; Ochs SM; Burgold S; Filser S; Steinbach S; Seeliger MW; Arzberger T; Goedert M; Kretzschmar HA; Schmidt B; Herms J. 2012. Long-term in vivo imaging of fibrillar tau in the retina of P301S transgenic mice. PLoS One 7(12):e53547. [PubMed: 23300938] [MGI Ref ID J:195824]
Stanwood GD; Parlaman JP; Levitt P. 2005. Anatomical abnormalities in dopaminoceptive regions of the cerebral cortex of dopamine D1 receptor mutant mice. J Comp Neurol 487(3):270-82. [PubMed: 15892099] [MGI Ref ID J:99709]
Stuss DP; Boyd JD; Levin DB; Delaney KR. 2012. MeCP2 mutation results in compartment-specific reductions in dendritic branching and spine density in layer 5 motor cortical neurons of YFP-H mice. PLoS One 7(3):e31896. [PubMed: 22412847] [MGI Ref ID J:186936]
Thomson SR; Nahon JE; Mutsaers CA; Thomson D; Hamilton G; Parson SH; Gillingwater TH. 2012. Morphological characteristics of motor neurons do not determine their relative susceptibility to degeneration in a mouse model of severe spinal muscular atrophy. PLoS One 7(12):e52605. [PubMed: 23285108] [MGI Ref ID J:195758]
Tomita K; Kubo T; Matsuda K; Yano K; Tohyama M; Hosokawa K. 2007. Myelin-associated glycoprotein reduces axonal branching and enhances functional recovery after sciatic nerve transection in rats. Glia 55(14):1498-507. [PubMed: 17705198] [MGI Ref ID J:156299]
Tsai J; Grutzendler J; Duff K; Gan WB. 2004. Fibrillar amyloid deposition leads to local synaptic abnormalities and breakage of neuronal branches. Nat Neurosci 7(11):1181-3. [PubMed: 15475950] [MGI Ref ID J:94364]
Vargas ME; Watanabe J; Singh SJ; Robinson WH; Barres BA. 2010. Endogenous antibodies promote rapid myelin clearance and effective axon regeneration after nerve injury. Proc Natl Acad Sci U S A 107(26):11993-8. [PubMed: 20547838] [MGI Ref ID J:161370]
Ventruti A; Kazdoba TM; Niu S; D'Arcangelo G. 2011. Reelin deficiency causes specific defects in the molecular composition of the synapses in the adult brain. Neuroscience 189:32-42. [PubMed: 21664258] [MGI Ref ID J:175650]
Wilhelm JC; Xu M; Cucoranu D; Chmielewski S; Holmes T; Lau KS; Bassell GJ; English AW. 2012. Cooperative roles of BDNF expression in neurons and Schwann cells are modulated by exercise to facilitate nerve regeneration. J Neurosci 32(14):5002-9. [PubMed: 22492055] [MGI Ref ID J:184131]
Wu SH; Arevalo JC; Sarti F; Tessarollo L; Gan WB; Chao MV. 2009. Ankyrin Repeat-rich Membrane Spanning/Kidins220 protein regulates dendritic branching and spine stability in vivo. Dev Neurobiol 69(9):547-57. [PubMed: 19449316] [MGI Ref ID J:162839]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry This strain originated on a mixed B6CBAF1 background. It has been backcrossed to C57BL/6J at least 5 times (8/14/00) Coat Color expected from breeding:Black, Albino mice have also been observed. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Hemizygous for Tg(Thy1-YFP)HJrs
Price per Pair (US dollars $) Pair Genotype $252.40 C57BL/6J (000664) x Hemizygous for Tg(Thy1-YFP)HJrs $250.90 Hemizygous for Tg(Thy1-YFP)HJrs x C57BL/6J (000664) Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Hemizygous for Tg(Thy1-YFP)HJrs
Price per Pair (US dollars $) Pair Genotype $328.20 C57BL/6J (000664) x Hemizygous for Tg(Thy1-YFP)HJrs $326.20 Hemizygous for Tg(Thy1-YFP)HJrs x C57BL/6J (000664) Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.