Strain Name:

B6.Cg-Tg(Lck-cre)548Jxm/J

Stock Number:

003802

Availability:

Repository- Live

Description

Strain Information

Former Names B6.Cg-TgN(Lck-Cre)548Jxm    (Changed: 15-DEC-04 )
B6.Cg-TgN(LckCre)548Jxm    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
GenerationN4+6N1F3 (10-AUG-09)
 
Donating Investigator Jamey Marth,   University of California at San Diego

Description
Homozygous mice are viable and have no major defects. This strain expresses Cre recombinase in thymocytes.

Development
A transgenic construct containing a Cre recombinase gene under the control of the Lck promoter was injected into B6DBA F1 zygotes and founder animals obtained.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Lck-cre)548Jxm allele
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
View Strains carrying   Tg(Lck-cre)548Jxm     (1 strain)

View Strains carrying other alleles of Lck     (6 strains)

Strains carrying other alleles of cre
004337   129(Cg)-Foxg1tm1(cre)Skm/J
008569   129-Alpltm1(cre)Nagy/J
003328   129-Tg(Prm-cre)58Og/J
005989   129;FVB-Tg(PTH-cre)4167Slib/J
007179   129S.Cg-Tg(UBC-cre/ESR1)1Ejb/J
007915   129S.FVB-Tg(Amh-cre)8815Reb/J
004302   129S1-Hprt1tm1(cre)Mnn/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
005697   B6.129-Otx1tm4(cre)Asim/J
004146   B6.129-Tg(Pcp2-cre)2Mpin/J
006785   B6.129P2(C)-Cd19tm1(cre)Cgn/J
006084   B6.129P2(Cg)-Foxg1tm1(cre)Skm/J
008710   B6.129P2-Hprt1tm10(Ple162-EGFP/cre)Ems/J
008877   B6.129P2-Hprt1tm12(Ple177-EGFP/cre)Ems/J
008709   B6.129P2-Hprt1tm9(Ple178-EGFP/cre)Ems/J
004781   B6.129P2-Lyz2tm1(cre)Ifo/J
005623   B6.129S-Shhtm2(cre/ESR1)Cjt/J
006600   B6.129S1-Mnx1tm4(cre)Tmj/J
005628   B6.129S2-Emx1tm1(cre)Krj/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
006878   B6.129S6-Taglntm2(cre)Yec/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
009642   B6.Cg(129)-Tg(Gh1-cre)1Sac/J
006230   B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
009616   B6.Cg-Tg(A930038C07Rik-cre)4Aibs/J
006149   B6.Cg-Tg(ACTA1-cre)79Jme/J
003574   B6.Cg-Tg(Alb-cre)21Mgn/J
006881   B6.Cg-Tg(Aqp2-cre)1Dek/J
004682   B6.Cg-Tg(CAG-cre/Esr1)5Amc/J
008520   B6.Cg-Tg(CD2-cre)4Kio/J
009350   B6.Cg-Tg(CDX2-cre)101Erf/J
009352   B6.Cg-Tg(CDX2-cre*)189Erf/J
005359   B6.Cg-Tg(Camk2a-cre)T29-1Stl/J
006137   B6.Cg-Tg(Cdh5-cre)7Mlia/J
006368   B6.Cg-Tg(Cr2-cre)3Cgn/J
006663   B6.Cg-Tg(Eno2-cre)39Jme/J
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
008781   B6.Cg-Tg(Kap-cre)29066/2Sig/J
006889   B6.Cg-Tg(Lck-cre)I540Jxm/J
009643   B6.Cg-Tg(Lhb-cre)1Sac/J
003556   B6.Cg-Tg(Mx1-cre)1Cgn/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
005657   B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J
008205   B6.Cg-Tg(NPHS2-cre)295Lbh/J
003771   B6.Cg-Tg(Nes-cre)1Kln/J
005975   B6.Cg-Tg(Plp1-cre/ESR1)3Pop/J
008827   B6.Cg-Tg(Prdm1-cre)1Masu/J
005584   B6.Cg-Tg(Prrx1-cre)1Cjt/J
003967   B6.Cg-Tg(Rbp3-cre)528Jxm/J
009613   B6.Cg-Tg(Scnn1a-cre)3Aibs/J
008454   B6.Cg-Tg(Sox2-cre)1Amc/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
003966   B6.Cg-Tg(Syn1-cre)671Jxm/J
004128   B6.Cg-Tg(Tek-cre)12Flv/J
008863   B6.Cg-Tg(Tek-cre)1Ywa/J
008601   B6.Cg-Tg(Th-cre)1Tmd/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
008085   B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J
008610   B6.Cg-Tg(Vav1-cre)A2Kio/J
008735   B6.Cg-Tg(Wap-cre)11738Mam/JKnwJ
009614   B6.Cg-Tg(Wfs1-cre/ERT2)2Aibs/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
006475   B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J
006451   B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J
006333   B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
003724   B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
003394   B6.FVB-Tg(Zp3-cre)3Mrt/J
006660   B6.SJL-Slc6a3tm1.1(cre)Bkmn/J
004586   B6.SJL-Tg(Vil-cre)997Gum/J
003552   B6129-Tg(Wap-cre)11738Mam/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
008876   B6;129-Hprt1tm11(Ple176-EGFP/cre)Ems/J
005549   B6;129-Pax3tm1(cre)Joe/J
005650   B6;129-Tg(Myh6-cre/Esr1)1Jmk/J
008529   B6;129P-Tg(Neurog1-cre/ESR1)1Good/J
006668   B6;129P2-Omptm4(cre)Mom/MomJ
007001   B6;129S-Tg(UBC-cre/ESR1)1Ejb/J
006410   B6;129S6-Chattm1(cre)Lowl/J
008844   B6;C3-Tg(Ctgf-cre)2Aibs/J
008839   B6;C3-Tg(Cyp39a1-cre)1Aibs/J
009117   B6;C3-Tg(Cyp39a1-cre)7Aibs/J
008848   B6;C3-Tg(Mybpc1-cre)2Aibs/J
009111   B6;C3-Tg(Scnn1a-cre)1Aibs/J
009112   B6;C3-Tg(Scnn1a-cre)2Aibs/J
009103   B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J
003466   B6;D2-Tg(Sycp1-cre)4Min/J
008533   B6;FVB-Tg(Cspg4-cre)1Akik/J
003734   B6;FVB-Tg(GZMB-cre)1Jcb/J
004426   B6;SJL-Tg(Cga-cre)3Sac/J
003554   B6;SJL-Tg(Col2a1-cre)1Bhr/J
005249   B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007252   B6Ei.129S4-Tg(Prm-cre)58Og/EiJ
003465   BALB/c-Tg(CMV-cre)1Cgn/J
004126   C.Cg-Cd19tm1(cre)Cgn Ighb/J
005673   C.Cg-Tg(Mx1-cre)1Cgn/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
008766   C57BL/6-Tg(Cd8a-cre)1Itan/J
006474   C57BL/6-Tg(Grik4-cre)G32-4Stl/J
008314   C57BL/6-Tg(HBB-cre)12Kpe/J
008870   C57BL/6-Tg(Hspa2-cre)1Eddy/J
008535   C57BL/6-Tg(Pf4-cre)Q3Rsko/J
006888   C57BL/6-Tg(Zp3-cre)1Gwh/J
003651   C57BL/6-Tg(Zp3-cre)93Knw/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
008661   C57BL/6J-Tg(Nkx2-1-cre)2Sand/J
006405   FVB-Tg(Ckmm-cre)5Khn/J
006774   FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J
006954   FVB-Tg(Ddx4-cre)1Dcas/J
004600   FVB-Tg(GFAP-cre)25Mes/J
006364   FVB-Tg(Nr5a1-cre)2Lowl/J
008537   FVB-Tg(Tek-cre)2352Rwng/J
006139   FVB.Cg-Tg(ACTA1-cre)79Jme/J
006297   FVB.Cg-Tg(Eno2-cre)39Jme/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
003376   FVB/N-Tg(ACTB-cre)2Mrt/J
003314   FVB/N-Tg(EIIa-cre)C5379Lmgd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
003377   FVB/N-Tg(Zp3-cre)3Mrt/J
008694   NOD/ShiLt-Tg(Foxp3-EGFP/cre)1Jbs/J
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt
008464   STOCK Foxa2tm2.1(cre/Esr1)Moon/J
004192   STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
006677   STOCK Olfr151tm28Mom/MomJ
007684   STOCK Tg(Atoh1-cre/ESR1)14Fsh/J
004453   STOCK Tg(CAG-cre/Esr1)5Amc/J
009615   STOCK Tg(Cartpt-cre)1Aibs/J
005105   STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J
008861   STOCK Tg(Ela1-Cre/ESR1)1Stof/J
005938   STOCK Tg(Eno2-cre)39Jme/J
004692   STOCK Tg(Hoxb7-cre)13Amc/J
008122   STOCK Tg(Ins2-cre/Esr1)1Dam/J
004782   STOCK Tg(KRT14-cre)1Amc/J
005107   STOCK Tg(KRT14-cre/Esr1)20Efu/J
008582   STOCK Tg(Kcnc2-Cre)K128Stl/LetJ
003551   STOCK Tg(MMTV-cre)1Mam/J
003553   STOCK Tg(MMTV-cre)4Mam/J
002527   STOCK Tg(Mx1-cre)1Cgn/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
009102   STOCK Tg(Nefh-cre)12Kul/J
002858   STOCK Tg(Nes-cre)1Wme/J
002859   STOCK Tg(Nes-cre)2Wme/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
008119   STOCK Tg(Neurog3-cre/Esr1)1Dam/J
006207   STOCK Tg(Pcp2-cre)1Amc/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
006395   STOCK Tg(Sim1-cre)1Lowl/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
004783   STOCK Tg(Sox2-cre)1Amc/J
008208   STOCK Tg(Stra8-cre)1Reb/J
004746   STOCK Tg(Tagln-cre)1Her/J
003829   STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
007807   STOCK Tg(Wnt1-cre)11Rth/MileJ
008199   STOCK Tg(dlx6a-cre)1Mekk/J
002471   STOCK Tg(hCMV-cre)140Sau/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of cre     (161 strains)

Additional Web Information

Introduction to Cre-lox technology
JAX® NOTES, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Research Tools
Cre-lox System
      Cre Recombinase Expression
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System

cre related

Research Tools
Cre-lox System
Genetics Research
      Mutagenesis and Transgenesis: Cre-lox System

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Lck-cre)548Jxm
Allele Name transgene insertion 548, Jamey Marth
Allele Type Transgenic (Cre/Flp)
Common Name(s) Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm;
Mutation Made By Jamey Marth,   University of California at San Diego
Strain of Origin(C57BL/6 x CBA)F2
Site of Expressionthymocytes
Expressed Gene cre, cre recombinase, bacteriophage P1
Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence.
Promoter Lck, lymphocyte protein tyrosine kinase, mouse, laboratory
Gene Symbol and Name Tg(Lck-cre)548Jxm, transgene insertion 548, Jamey Marth
Chromosome UN
Gene Common Name(s) Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm;
Driver Note Lck
General Note Homozygous transgenic mice are viable and have no major defects.
Molecular Note This transgene expresses Cre recombinase under the control of the mouse lck promoter, active in the T cell lineage. [MGI Ref ID J:67674]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cre Quantitative PCR, QPCR
Generic Cre, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Hennet T; Hagen FK; Tabak LA; Marth JD. 1995. T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination. Proc Natl Acad Sci U S A 92(26):12070-4. [PubMed: 8618846]  [MGI Ref ID J:67674]

Additional References

Garvin AM; Pawar S; Marth JD; Perlmutter RM. 1988. Structure of the murine lck gene and its rearrangement in a murine lymphoma cell line. Mol Cell Biol 8(8):3058-64. [PubMed: 2850479]  [MGI Ref ID J:9539]

McPherson JP; Lemmers B; Hirao A; Hakem A; Abraham J; Migon E; Matysiak-Zablocki E; Tamblyn L; Sanchez-Sweatman O; Khokha R; Squire J; Hande MP; Mak TW; Hakem R. 2004. Collaboration of Brca1 and Chk2 in tumorigenesis. Genes Dev 18(10):1144-53. [PubMed: 15131084]  [MGI Ref ID J:90512]

Orban PC; Chui D; Marth JD. 1992. Tissue- and site-specific DNA recombination in transgenic mice. Proc Natl Acad Sci U S A 89(15):6861-5. [PubMed: 1495975]  [MGI Ref ID J:75414]

Salmena L; Lemmers B; Hakem A; Matysiak-Zablocki E; Murakami K; Au PY; Berry DM; Tamblyn L; Shehabeldin A; Migon E; Wakeham A; Bouchard D; Yeh WC; McGlade JC; Ohashi PS; Hakem R. 2003. Essential role for caspase 8 in T-cell homeostasis and T-cell-mediated immunity. Genes Dev 17(7):883-95. [PubMed: 12654726]  [MGI Ref ID J:82759]

Tg(Lck-cre)548Jxm related

Azhar M; Yin M; Bommireddy R; Duffy JJ; Yang J; Pawlowski SA; Boivin GP; Engle SJ; Sanford LP; Grisham C; Singh RR; Babcock GF; Doetschman T. 2009. Generation of mice with a conditional allele for transforming growth factor beta 1 gene. Genesis 47(6):423-31. [PubMed: 19415629]  [MGI Ref ID J:150024]

Bender TP; Kremer CS; Kraus M; Buch T; Rajewsky K. 2004. Critical functions for c-Myb at three checkpoints during thymocyte development. Nat Immunol 5(7):721-9. [PubMed: 15195090]  [MGI Ref ID J:91122]

Brewer JA; Khor B; Vogt SK; Muglia LM; Fujiwara H; Haegele KE; Sleckman BP; Muglia LJ. 2003. T-cell glucocorticoid receptor is required to suppress COX-2-mediated lethal immune activation. Nat Med 9(10):1318-22. [PubMed: 12949501]  [MGI Ref ID J:97484]

Cheng Y; Zhang Z; Slape C; Aplan PD. 2007. Cre-loxP-mediated recombination between the SIL and SCL genes leads to a block in T-cell development at the CD4- CD8- to CD4+ CD8+ transition. Neoplasia 9(4):315-21. [PubMed: 17460775]  [MGI Ref ID J:123860]

Cheung AM; Hande MP; Jalali F; Tsao MS; Skinnider B; Hirao A; McPherson JP; Karaskova J; Suzuki A; Wakeham A; You-Ten A; Elia A; Squire J; Bristow R; Hakem R; Mak TW. 2002. Loss of Brca2 and p53 Synergistically Promotes Genomic Instability and Deregulation of T-cell Apoptosis. Cancer Res 62(21):6194-204. [PubMed: 12414647]  [MGI Ref ID J:79697]

Cully M; Elia A; Ong SH; Stambolic V; Pawson T; Tsao MS; Mak TW. 2004. grb2 heterozygosity rescues embryonic lethality but not tumorigenesis in pten+/- mice. Proc Natl Acad Sci U S A 101(43):15358-63. [PubMed: 15492213]  [MGI Ref ID J:93530]

Dougan SK; Rava P; Hussain MM; Blumberg RS. 2007. MTP regulated by an alternate promoter is essential for NKT cell development. J Exp Med 204(3):533-45. [PubMed: 17312007]  [MGI Ref ID J:125365]

Dzhagalov I; Dunkle A; He YW. 2008. The anti-apoptotic Bcl-2 family member Mcl-1 promotes T lymphocyte survival at multiple stages. J Immunol 181(1):521-8. [PubMed: 18566418]  [MGI Ref ID J:137400]

Eddahri F; Denanglaire S; Bureau F; Spolski R; Leonard WJ; Leo O; Andris F. 2009. Interleukin-6/STAT3 signaling regulates the ability of naive T cells to acquire B-cell help capacities. Blood 113(11):2426-33. [PubMed: 19020307]  [MGI Ref ID J:146334]

Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. [PubMed: 19433445]  [MGI Ref ID J:150146]

Gebuhr TC; Kovalev GI; Bultman S; Godfrey V; Su L; Magnuson T. 2003. The role of Brg1, a catalytic subunit of mammalian chromatin-remodeling complexes, in T cell development. J Exp Med 198(12):1937-49. [PubMed: 14676303]  [MGI Ref ID J:87219]

Herroeder S; Reichardt P; Sassmann A; Zimmermann B; Jaeneke D; Hoeckner J; Hollmann MW; Fischer KD; Vogt S; Grosse R; Hogg N; Gunzer M; Offermanns S; Wettschureck N. 2009. Guanine nucleotide-binding proteins of the G12 family shape immune functions by controlling CD4+ T cell adhesiveness and motility. Immunity 30(5):708-20. [PubMed: 19409815]  [MGI Ref ID J:149560]

Hock H; Meade E; Medeiros S; Schindler JW; Valk PJ; Fujiwara Y; Orkin SH. 2004. Tel/Etv6 is an essential and selective regulator of adult hematopoietic stem cell survival. Genes Dev 18(19):2336-41. [PubMed: 15371326]  [MGI Ref ID J:93052]

Jin J; Desai BN; Navarro B; Donovan A; Andrews NC; Clapham DE. 2008. Deletion of Trpm7 disrupts embryonic development and thymopoiesis without altering Mg2+ homeostasis. Science 322(5902):756-60. [PubMed: 18974357]  [MGI Ref ID J:140630]

Kasper LH; Fukuyama T; Biesen MA; Boussouar F; Tong C; de Pauw A; Murray PJ; van Deursen JM; Brindle PK. 2006. Conditional knockout mice reveal distinct functions for the global transcriptional coactivators CBP and p300 in T-cell development. Mol Cell Biol 26(3):789-809. [PubMed: 16428436]  [MGI Ref ID J:105505]

King CG; Kobayashi T; Cejas PJ; Kim T; Yoon K; Kim GK; Chiffoleau E; Hickman SP; Walsh PT; Turka LA; Choi Y. 2006. TRAF6 is a T cell-intrinsic negative regulator required for the maintenance of immune homeostasis. Nat Med 12(9):1088-92. [PubMed: 16921377]  [MGI Ref ID J:131909]

Kishimoto H; Ohteki T; Yajima N; Kawahara K; Natsui M; Kawarasaki S; Hamada K; Horie Y; Kubo Y; Arase S; Taniguchi M; Vanhaesebroeck B; Mak TW; Nakano T; Koyasu S; Sasaki T; Suzuki A. 2007. The Pten/PI3K pathway governs the homeostasis of Valpha14iNKT cells. Blood 109(8):3316-24. [PubMed: 17170126]  [MGI Ref ID J:145339]

Lieu YK; Kumar A; Pajerowski AG; Rogers TJ; Reddy EP. 2004. Requirement of c-myb in T cell development and in mature T cell function. Proc Natl Acad Sci U S A 101(41):14853-8. [PubMed: 15466706]  [MGI Ref ID J:93481]

Liu HH; Xie M; Schneider MD; Chen ZJ. 2006. Essential role of TAK1 in thymocyte development and activation. Proc Natl Acad Sci U S A 103(31):11677-82. [PubMed: 16857737]  [MGI Ref ID J:111794]

Liu Y; Zhang P; Li J; Kulkarni AB; Perruche S; Chen W. 2008. A critical function for TGF-beta signaling in the development of natural CD4+CD25+Foxp3+ regulatory T cells. Nat Immunol 9(6):632-40. [PubMed: 18438410]  [MGI Ref ID J:136291]

Luche H; Weber O; Nageswara Rao T; Blum C; Fehling HJ. 2007. Faithful activation of an extra-bright red fluorescent protein in 'knock-in' Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol 37(1):43-53. [PubMed: 17171761]  [MGI Ref ID J:117041]

Mak TW; Hakem A; McPherson JP; Shehabeldin A; Zablocki E; Migon E; Duncan GS; Bouchard D; Wakeham A; Cheung A; Karaskova J; Sarosi I; Squire J; Marth J; Hakem R. 2000. Brcal required for T cell lineage development but not TCR loci rearrangement. Nat Immunol 1(1):77-82. [PubMed: 10881179]  [MGI Ref ID J:63282]

Maresz K; Pryce G; Ponomarev ED; Marsicano G; Croxford JL; Shriver LP; Ledent C; Cheng X; Carrier EJ; Mann MK; Giovannoni G; Pertwee RG; Yamamura T; Buckley NE; Hillard CJ; Lutz B; Baker D; Dittel BN. 2007. Direct suppression of CNS autoimmune inflammation via the cannabinoid receptor CB1 on neurons and CB2 on autoreactive T cells. Nat Med 13(4):492-7. [PubMed: 17401376]  [MGI Ref ID J:129619]

McPherson JP; Hande MP; Poonepalli A; Lemmers B; Zablocki E; Migon E; Shehabeldin A; Porras A; Karaskova J; Vukovic B; Squire J; Hakem R. 2006. A role for Brca1 in chromosome end maintenance. Hum Mol Genet 15(6):831-8. [PubMed: 16446310]  [MGI Ref ID J:106759]

McPherson JP; Lemmers B; Hirao A; Hakem A; Abraham J; Migon E; Matysiak-Zablocki E; Tamblyn L; Sanchez-Sweatman O; Khokha R; Squire J; Hande MP; Mak TW; Hakem R. 2004. Collaboration of Brca1 and Chk2 in tumorigenesis. Genes Dev 18(10):1144-53. [PubMed: 15131084]  [MGI Ref ID J:90512]

Mei Y; Zhang Y; Yamamoto K; Xie W; Mak TW; You H. 2009. FOXO3a-dependent regulation of Pink1 (Park6) mediates survival signaling in response to cytokine deprivation. Proc Natl Acad Sci U S A 106(13):5153-8. [PubMed: 19276113]  [MGI Ref ID J:147146]

Miwa T; Zhou L; Kimura Y; Kim D; Bhandoola A; Song WC. 2009. Complement-dependent T-cell lymphopenia caused by thymocyte deletion of the membrane complement regulator Crry. Blood 113(12):2684-94. [PubMed: 19136662]  [MGI Ref ID J:146538]

Nguyen TV; Ke Y; Zhang EE; Feng GS. 2006. Conditional deletion of Shp2 tyrosine phosphatase in thymocytes suppresses both pre-TCR and TCR signals. J Immunol 177(9):5990-6. [PubMed: 17056523]  [MGI Ref ID J:140530]

O'Donnell N; Zachara NE; Hart GW; Marth JD. 2004. Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol Cell Biol 24(4):1680-90. [PubMed: 14749383]  [MGI Ref ID J:93112]

Pan L; Bradney C; Zheng B; Zhuang Y. 2004. Altered T-dependent antigen responses and development of autoimmune symptoms in mice lacking E2A in T lymphocytes. Immunology 111(2):147-54. [PubMed: 15027899]  [MGI Ref ID J:88415]

Panattoni M; Sanvito F; Basso V; Doglioni C; Casorati G; Montini E; Bender JR; Mondino A; Pardi R. 2008. Targeted inactivation of the COP9 signalosome impairs multiple stages of T cell development. J Exp Med 205(2):465-77. [PubMed: 18268034]  [MGI Ref ID J:132114]

Perruche S; Zhang P; Maruyama T; Bluestone JA; Saas P; Chen W. 2009. Lethal effect of CD3-specific antibody in mice deficient in TGF-beta1 by uncontrolled flu-like syndrome. J Immunol 183(2):953-61. [PubMed: 19561097]  [MGI Ref ID J:151662]

Rebholz B; Haase I; Eckelt B; Paxian S; Flaig MJ; Ghoreschi K; Nedospasov SA; Mailhammer R; Debey-Pascher S; Schultze JL; Weindl G; Forster I; Huss R; Stratis A; Ruzicka T; Rocken M; Pfeffer K; Schmid RM; Rupec RA. 2007. Crosstalk between Keratinocytes and Adaptive Immune Cells in an IkappaBalpha Protein-Mediated Inflammatory Disease of the Skin. Immunity 27(2):296-307. [PubMed: 17692539]  [MGI Ref ID J:124343]

Sagiv Y; Bai L; Wei DG; Agami R; Savage PB; Teyton L; Bendelac A. 2007. A distal effect of microsomal triglyceride transfer protein deficiency on the lysosomal recycling of CD1d. J Exp Med 204(4):921-8. [PubMed: 17403933]  [MGI Ref ID J:125608]

Salmena L; Hakem R. 2005. Caspase-8 deficiency in T cells leads to a lethal lymphoinfiltrative immune disorder. J Exp Med 202(6):727-32. [PubMed: 16157684]  [MGI Ref ID J:107455]

Salmena L; Lemmers B; Hakem A; Matysiak-Zablocki E; Murakami K; Au PY; Berry DM; Tamblyn L; Shehabeldin A; Migon E; Wakeham A; Bouchard D; Yeh WC; McGlade JC; Ohashi PS; Hakem R. 2003. Essential role for caspase 8 in T-cell homeostasis and T-cell-mediated immunity. Genes Dev 17(7):883-95. [PubMed: 12654726]  [MGI Ref ID J:82759]

Schmedt C; Tarakhovsky A. 2001. Autonomous maturation of alpha/beta T lineage cells in the absence of COOH-terminal Src kinase (Csk). J Exp Med 193(7):815-26. [PubMed: 11283154]  [MGI Ref ID J:120548]

Shui JW; Hu MC; Tan TH. 2007. Conditional knockout mice reveal an essential role of protein phosphatase 4 in thymocyte development and pre-T-cell receptor signaling. Mol Cell Biol 27(1):79-91. [PubMed: 17060460]  [MGI Ref ID J:117684]

Stephensen CB; Borowsky AD; Lloyd KC. 2007. Disruption of Rxra gene in thymocytes and T lymphocytes modestly alters lymphocyte frequencies, proliferation, survival and T helper type 1/type 2 balance. Immunology 121(4):484-98. [PubMed: 17433077]  [MGI Ref ID J:125537]

Stranges PB; Watson J; Cooper CJ; Choisy-Rossi CM; Stonebraker AC; Beighton RA; Hartig H; Sundberg JP; Servick S; Kaufmann G; Fink PJ; Chervonsky AV. 2007. Elimination of antigen-presenting cells and autoreactive T cells by Fas contributes to prevention of autoimmunity. Immunity 26(5):629-41. [PubMed: 17509906]  [MGI Ref ID J:123556]

Suzuki A; Nakano T; Mak TW; Sasaki T. 2008. Portrait of PTEN: messages from mutant mice. Cancer Sci 99(2):209-13. [PubMed: 18201277]  [MGI Ref ID J:142484]

Suzuki A; Yamaguchi MT; Ohteki T; Sasaki T; Kaisho T; Kimura Y; Yoshida R; Wakeham A; Higuchi T; Fukumoto M; Tsubata T; Ohashi PS; Koyasu S; Penninger JM; Nakano T; Mak TW. 2001. T cell-specific loss of pten leads to defects in central and peripheral tolerance. Immunity 14(5):523-34. [PubMed: 11371355]  [MGI Ref ID J:69471]

Ventura A; Meissner A; Dillon CP; McManus M; Sharp PA; Van Parijs L; Jaenisch R; Jacks T. 2004. Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci U S A 101(28):10380-5. [PubMed: 15240889]  [MGI Ref ID J:129426]

Wang X; Chang X; Facchinetti V; Zhuang Y; Su B. 2009. MEKK3 is essential for lymphopenia-induced T cell proliferation and survival. J Immunol 182(6):3597-608. [PubMed: 19265138]  [MGI Ref ID J:145923]

Weischenfeldt J; Damgaard I; Bryder D; Theilgaard-Monch K; Thoren LA; Nielsen FC; Jacobsen SE; Nerlov C; Porse BT. 2008. NMD is essential for hematopoietic stem and progenitor cells and for eliminating by-products of programmed DNA rearrangements. Genes Dev 22(10):1381-96. [PubMed: 18483223]  [MGI Ref ID J:134765]

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Xue L; Nolla H; Suzuki A; Mak TW; Winoto A. 2008. Normal development is an integral part of tumorigenesis in T cell-specific PTEN-deficient mice. Proc Natl Acad Sci U S A 105(6):2022-7. [PubMed: 18250301]  [MGI Ref ID J:131807]

You H; Pellegrini M; Tsuchihara K; Yamamoto K; Hacker G; Erlacher M; Villunger A; Mak TW. 2006. FOXO3a-dependent regulation of Puma in response to cytokine/growth factor withdrawal. J Exp Med 203(7):1657-63. [PubMed: 16801400]  [MGI Ref ID J:124403]

Young JA; Becker AM; Medeiros JJ; Shapiro VS; Wang A; Farrar JD; Quill TA; van Huijsduijnen RH; van Oers NS. 2008. The protein tyrosine phosphatase PTPN4/PTP-MEG1, an enzyme capable of dephosphorylating the TCR ITAMs and regulating NF-kappaB, is dispensable for T cell development and/or T cell effector functions. Mol Immunol 45(14):3756-66. [PubMed: 18614237]  [MGI Ref ID J:138871]

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Zhang N; He YW. 2005. The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol 174(11):6967-73. [PubMed: 15905539]  [MGI Ref ID J:99012]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6DBAF1 background. It has been backcrossed to C57BL/6 at least four times. Coat color expected from breeding:Black
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $110.20Female or MaleHomozygous for Tg(Lck-cre)548Jxm
Pairs /Price (US dollars $)Pair Genotype
$220.40Homozygous for Tg(Lck-cre)548Jxm x Homozygous for Tg(Lck-cre)548Jxm

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice (US dollars $)GenderGenotypes Provided
Individual Mouse $143.30Female or MaleHomozygous for Tg(Lck-cre)548Jxm
Pairs /Price (US dollars $)Pair Genotype
$286.60Homozygous for Tg(Lck-cre)548Jxm x Homozygous for Tg(Lck-cre)548Jxm

Additional Supply Details

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement.
Supply Notes

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
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Tel: 1-800-422-6423 or 1-207-288-5845
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Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

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phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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