Strain Name: |
B6.Cg-Tg(Lck-cre)548Jxm/J |
|---|---|
Stock Number: |
003802 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-TgN(Lck-Cre)548Jxm (Changed: 15-DEC-04
) |
|
B6.Cg-TgN(LckCre)548Jxm (Changed: 15-DEC-04
) | |
| Genes & Alleles | Lck; cre; Tg(Lck-cre)548Jxm; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Jamey Marth, University of California at San Diego Generation N4+6F9N1F1 (06-DEC-07) Strain Description
Homozygous mice are viable and have no major defects. This strain expresses Cre recombinase in thymocytes.Strain Development
A transgenic construct containing a Cre recombinase gene under the control of the Lck promoter was injected into B6DBA F1 zygotes and founder animals obtained.
| Allele Symbol | Tg(Lck-cre)548Jxm | ||
|---|---|---|---|
| Allele Name | transgene insertion 548, Jamey Marth | ||
| Common Name(s) | Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm; | ||
| Mutation Made By | Jamey Marth, University of California at San Diego | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | thymocytes | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| General Note | Homozygous transgenic mice are viable and have no major defects. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the mouse lck promoter, active in the T cell lineage. [MGI Ref ID J:67674] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Generic Cre
Generic Cre QPCR
| Breeding & Husbandry | This strain originated on a B6DBAF1 background. It has been backcrossed to C57BL/6 at least four times. Coat color expected from breeding:Black |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Strains carrying Tg(Lck-cre)548Jxm allele
005732 NOD.Cg-Tg(Lck-cre)548Jxm/AchJ View Strains carrying Tg(Lck-cre)548Jxm (1 strain)
Strains carrying other alleles of Lck
002817 B6.129S2-Lcktm1Mak/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 003332 C57BL/6-Tg(LckNotch1)9Erob/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J View Strains carrying other alleles of Lck (4 strains)
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (114 strains)
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
JAX Notes, Summer 2001; 482. Cre Transgenic Strains for Conditional Mutagenesis.
Room Number AX12
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Selected Reference(s)
Additional ReferencesHennet T; Hagen FK; Tabak LA; Marth JD. 1995. T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination. Proc Natl Acad Sci U S A 92(26):12070-4. [PubMed: 8618846] [MGI Ref ID J:67674]
| Strain Name: | B6.Cg-Tg(Lck-cre)548Jxm/J |
| Stock Number: | 003802 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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