Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation ?+2p   Donating Investigator Richard Hynes,   Massachusetts Institute of Technology

Description
Mice that are homozygous null for the Sele and Sell genes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Sele or Sell gene products (mRNA or protein) are detected. A slightly diminished response in neutrophil recruitment to the peritoneum in response to thioglycollate is observed, as is a diminished ability to interact with the venular endothelium resulting in increased "rolling" along the vessel wall. These mice are suitable for use in research applications studying leukocyte homeostasis, infectious diseases and inflammation.

Development
Sequential mutation by homologous recombination was utilized to replace the Sele and Sell genes with drug-resistance genes for hygromycin and puromycin (respectively) in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This approach was necessary because the Sele and Sell genes are too closely linked for double-deficient animals to be generated by mating single-deficient animals. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric offspring obtained.

Related Strains

Strains carrying   Sele^tm1Hyn allele

008434	B6.129S2-Seletm1Hyn/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
002915	B6;129S2-Seletm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J
008435	C.129S2(B6)-Seletm1Hyn/J

View Strains carrying   Sele^tm1Hyn     (6 strains)

Strains carrying   Sell^tm1Hyn allele

008436	B6.129S2-Selltm1Hyn/J
008441	B6.129S2-Selptm1Hyn Selltm1Hyn/J
003807	B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
003805	B6;129S-Selptm1Hyn Selltm1Hyn/J
002917	B6;129S2-Selltm1Hyn/J
008442	C.129S2(B6)-Selptm1Hyn Selltm1Hyn/J

View Strains carrying   Sell^tm1Hyn     (6 strains)

Strains carrying other alleles of Sele

008238	129S-Seletm1Dmil/J
008236	B6.129S4-Seletm1Dmil/J
008237	C.129S4-Seletm1Dmil/J

View Strains carrying other alleles of Sele     (3 strains)

Strains carrying other alleles of Sell

003860	NOD.129P2(B6)-Selltm1Tft/1LtJ
004943	NOD.Cg-Selltm1Tft Itgb7tm1Cgn/LtJ

View Strains carrying other alleles of Sell     (2 strains)

Phenotype

Phenotype Information

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Sele^tm1Hyn related

Cardiovascular Research
Atherosclerosis
Vascular Defects (defective leukocyte function)

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules

Hematological Research
Hematopoietic Defects
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency Associated with Other Defects
Inflammation

Research Tools
Cell Biology Research
Immunology and Inflammation Research
Metabolism Research

Sell^tm1Hyn related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Seletm1Hyn
Allele Name		targeted mutation 1, Richard O Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		E-selectin-; Sele-;
Mutation Made By		Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Sele, selectin, endothelial cell
Chromosome		1
Gene Common Name(s)		CD62E; E-selectin; ELAM; ELAM1; ESEL; Elam; LECAM2; endothelial adhesion molecule;
General Note		A modified D3 ES cell line heterozygous for a Selp mutation was used.
Molecular Note		The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal message in extracts from cardiac and pulmonary tissue of homozygous mutant mice. [MGI Ref ID J:31626]
Allele Symbol		Selltm1Hyn
Allele Name		targeted mutation 1, Richard Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		L-Sel-; L-;
Mutation Made By		Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Sell, selectin, lymphocyte
Chromosome		1
Gene Common Name(s)		A.11; AI528707; CD62L; L-selectin; LAM-1; LAM1; LECAM-1; LECAM1; LNHR; LSEL; LYAM1; Leu-8; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1; PLNHR; TQ1; expressed sequence AI528707; hLHRc; lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; lymphocyte antigen m22;
Molecular Note		A PGK-puro cassette was used to delete a 2.1kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of encoded protein in leukocytes isolated from homozygous mutant mice. Northern blot and Western blot analysis showed the transcript and protein levels of endothelial and platelet selectins to be unaffected by the targeting event. [MGI Ref ID J:57973]

Genotyping

Genotyping Information

Genotyping Protocols

Sele^tm1Hyn, STD PCR, vers. 1
Sell^tm1Hyn, STD PCR, vers. 2

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Sele^tm1Hyn related

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Frenette J; Chbinou N; Godbout C; Marsolais D; Frenette PS. 2003. Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading. Am J Physiol Regul Integr Comp Physiol 285(4):R727-32. [PubMed: 12829442]  [MGI Ref ID J:109393]

Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043]  [MGI Ref ID J:31626]

Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58. [PubMed: 17456770]  [MGI Ref ID J:121083]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Ginhoux F; Collin MP; Bogunovic M; Abel M; Leboeuf M; Helft J; Ochando J; Kissenpfennig A; Malissen B; Grisotto M; Snoeck H; Randolph G; Merad M. 2007. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204(13):3133-46. [PubMed: 18086862]  [MGI Ref ID J:130817]

Hidalgo A; Peired AJ; Wild MK; Vestweber D; Frenette PS. 2007. Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4):477-89. [PubMed: 17442598]  [MGI Ref ID J:123577]

Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362]  [MGI Ref ID J:107382]

Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773]  [MGI Ref ID J:50212]

Horikawa M; Fujimoto M; Hasegawa M; Matsushita T; Hamaguchi Y; Kawasuji A; Matsushita Y; Fujita T; Ogawa F; Takehara K; Steeber DA; Sato S. 2006. E- and P-selectins synergistically inhibit bleomycin-induced pulmonary fibrosis. Am J Pathol 169(3):740-9. [PubMed: 16936251]  [MGI Ref ID J:112363]

Kaifi JT; Hall LR; Diaz C; Sypek J; Diaconu E; Lass JH; Pearlman E. 2000. Impaired eosinophil recruitment to the cornea in P-selectin-deficient mice in onchocerca volvulus keratitis (River blindness) Invest Ophthalmol Vis Sci 41(12):3856-61. [PubMed: 11053286]  [MGI Ref ID J:65551]

Komura K; Hasegawa M; Hamaguchi Y; Saito E; Kaburagi Y; Yanaba K; Kawara S; Takehara K; Seki M; Steeber DA; Tedder TF; Sato S. 2003. Ultraviolet light exposure suppresses contact hypersensitivity by abrogating endothelial intercellular adhesion molecule-1 up-regulation at the elicitation site. J Immunol 171(6):2855-62. [PubMed: 12960307]  [MGI Ref ID J:85377]

Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599]  [MGI Ref ID J:134224]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Tang T; Frenette PS; Hynes RO; Wagner DD; Mayadas TN. 1996. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest 97(11):2485-90. [PubMed: 8647940]  [MGI Ref ID J:107411]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Yanaba K; Kaburagi Y; Takehara K; Steeber DA; Tedder TF; Sato S. 2003. Relative contributions of selectins and intercellular adhesion molecule-1 to tissue injury induced by immune complex deposition. Am J Pathol 162(5):1463-73. [PubMed: 12707029]  [MGI Ref ID J:113592]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Sell^tm1Hyn related

Angeli V; Ginhoux F; Llodra J; Quemeneur L; Frenette PS; Skobe M; Jessberger R; Merad M; Randolph GJ. 2006. B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilization. Immunity 24(2):203-15. [PubMed: 16473832]  [MGI Ref ID J:113320]

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Chen S; Kawashima H; Lowe JB; Lanier LL; Fukuda M. 2005. Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment. J Exp Med 202(12):1679-89. [PubMed: 16352740]  [MGI Ref ID J:118830]

Csencsits KL; Pascual DW. 2002. Absence of L-selectin delays mucosal B cell responses in nonintestinal effector tissues. J Immunol 169(10):5649-59. [PubMed: 12421944]  [MGI Ref ID J:80056]

Csencsits KL; Walters N; Pascual DW. 2001. Cutting edge: dichotomy of homing receptor dependence by mucosal effector B cells: alpha(E) versus L-selectin. J Immunol 167(5):2441-5. [PubMed: 11509580]  [MGI Ref ID J:93848]

Czarneski J; Berguer P; Bekinschtein P; Kim DC; Hakimpour P; Wagner N; Nepomnaschy I; Piazzon I; Ross SR. 2002. Neonatal infection with a milk-borne virus is independent of beta7 integrin- and L-selectin-expressing lymphocytes. Eur J Immunol 32(4):945-56. [PubMed: 11920560]  [MGI Ref ID J:76001]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Eriksson EE; Xie X; Werr J; Thoren P; Lindbom L. 2001. Importance of primary capture and L-selectin-dependent secondary capture in leukocyte accumulation in inflammation and atherosclerosis in vivo. J Exp Med 194(2):205-18. [PubMed: 11457895]  [MGI Ref ID J:118726]

Fukuyama S; Hiroi T; Yokota Y; Rennert PD; Yanagita M; Kinoshita N; Terawaki S; Shikina T; Yamamoto M; Kurono Y; Kiyono H. 2002. Initiation of NALT organogenesis is independent of the IL-7R, LTbetaR, and NIK signaling pathways but requires the Id2 gene and CD3(-)CD4(+)CD45(+) cells. Immunity 17(1):31-40. [PubMed: 12150889]  [MGI Ref ID J:78101]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Gonda A; Gal I; Szanto S; Sarraj B; Glant TT; Hunyadi J; Mikecz K. 2005. CD44, but not l-selectin, is critically involved in leucocyte migration into the skin in a murine model of allergic dermatitis. Exp Dermatol 14(9):700-8. [PubMed: 16098130]  [MGI Ref ID J:114317]

Ho J; Kurtz CC; Naganuma M; Ernst PB; Cominelli F; Rivera-Nieves J. 2008. A CD8+/CD103high T cell subset regulates TNF-mediated chronic murine ileitis. J Immunol 180(4):2573-80. [PubMed: 18250468]  [MGI Ref ID J:131978]

Laubli H; Stevenson JL; Varki A; Varki NM; Borsig L. 2006. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res 66(3):1536-42. [PubMed: 16452210]  [MGI Ref ID J:106671]

Lawson JA; Burns AR; Farhood A; Lynn Bajt M; Collins RG; Smith CW; Jaeschke H. 2000. Pathophysiologic importance of E- and L-selectin for neutrophil-induced liver injury during endotoxemia in mice. Hepatology 32(5):990-8. [PubMed: 11050049]  [MGI Ref ID J:106550]

Lejtenyi D; Osmond DG; Miller SC. 2003. Natural killer cells and B lymphocytes in L-selectin and Mac-1/LFA-1 knockout mice: marker-dependent, but not cell lineage-dependent changes in the spleen and bone marrow. Immunobiology 207(2):129-35. [PubMed: 12675270]  [MGI Ref ID J:102758]

Li O; Liu JQ; Zhang H; Zheng P; Liu Y; Bai XF. 2006. CD62L is required for the priming of encephalitogenic T cells but does not play a major role in the effector phase of experimental autoimmune encephalomyelitis. Scand J Immunol 64(2):117-24. [PubMed: 16867156]  [MGI Ref ID J:135873]

Martin AP; Coronel EC; Sano G; Chen SC; Vassileva G; Canasto-Chibuque C; Sedgwick JD; Frenette PS; Lipp M; Furtado GC; Lira SA. 2004. A novel model for lymphocytic infiltration of the thyroid gland generated by transgenic expression of the CC chemokine CCL21. J Immunol 173(8):4791-8. [PubMed: 15470018]  [MGI Ref ID J:93715]

Pascual DW; Wang X; Kochetkova I; Callis G; Riccardi C. 2008. The absence of lymphoid CD8+ dendritic cell maturation in L-selectin-/- respiratory compartment attenuates antiviral immunity. J Immunol 181(2):1345-56. [PubMed: 18606689]  [MGI Ref ID J:137476]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Sarraj B; Ludanyi K; Glant TT; Finnegan A; Mikecz K. 2006. Expression of CD44 and L-selectin in the innate immune system is required for severe joint inflammation in the proteoglycan-induced murine model of rheumatoid arthritis. J Immunol 177(3):1932-40. [PubMed: 16849507]  [MGI Ref ID J:138358]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Szanto S; Gal I; Gonda A; Glant TT; Mikecz K. 2004. Expression of L-selectin, but not CD44, is required for early neutrophil extravasation in antigen-induced arthritis. J Immunol 172(11):6723-34. [PubMed: 15153489]  [MGI Ref ID J:90526]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Wang L; Brown JR; Varki A; Esko JD. 2002. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest 110(1):127-36. [PubMed: 12093896]  [MGI Ref ID J:112426]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain originated on a B6;129S2 background and is maintained as a homozygote. The donating lab maintained these mice by avoiding brother-sister matings.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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