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Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation ?+2p Donating Investigator Richard Hynes, Massachusetts Institute of Technology Description
Mice that are homozygous null for the Sele and Sell genes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No Sele or Sell gene products (mRNA or protein) are detected. A slightly diminished response in neutrophil recruitment to the peritoneum in response to thioglycollate is observed, as is a diminished ability to interact with the venular endothelium resulting in increased "rolling" along the vessel wall. These mice are suitable for use in research applications studying leukocyte homeostasis, infectious diseases and inflammation.Development
Sequential mutation by homologous recombination was utilized to replace the Sele and Sell genes with drug-resistance genes for hygromycin and puromycin (respectively) in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This approach was necessary because the Sele and Sell genes are too closely linked for double-deficient animals to be generated by mating single-deficient animals. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric offspring obtained.
Strains carrying Seletm1Hyn allele
008434 B6.129S2-Seletm1Hyn/J 003807 B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J 002916 B6;129S2-Seletm1Hyn Selptm1Hyn/J 002915 B6;129S2-Seletm1Hyn/J 008438 C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J 008435 C.129S2(B6)-Seletm1Hyn/J View Strains carrying Seletm1Hyn (6 strains)
Strains carrying Selltm1Hyn allele
008436 B6.129S2-Selltm1Hyn/J 008441 B6.129S2-Selptm1Hyn Selltm1Hyn/J 003807 B6;129S-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J 003805 B6;129S-Selptm1Hyn Selltm1Hyn/J 002917 B6;129S2-Selltm1Hyn/J 008442 C.129S2(B6)-Selptm1Hyn Selltm1Hyn/J View Strains carrying Selltm1Hyn (6 strains)
Strains carrying other alleles of Sele
008238 129S-Seletm1Dmil/J 008236 B6.129S4-Seletm1Dmil/J 008237 C.129S4-Seletm1Dmil/J View Strains carrying other alleles of Sele (3 strains)
Strains carrying other alleles of Sell
003860 NOD.129P2(B6)-Selltm1Tft/1LtJ 004943 NOD.Cg-Selltm1Tft Itgb7tm1Cgn/LtJ View Strains carrying other alleles of Sell (2 strains)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Seletm1Hyn related
Selltm1Hyn relatedCardiovascular Research
Atherosclerosis
Vascular Defects (defective leukocyte function)
Cell Biology Research
Defects in Cell Adhesion Molecules
Developmental Biology Research
Defects in Cell Adhesion Molecules
Hematological Research
Hematopoietic Defects
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency Associated with Other Defects
Inflammation
Research Tools
Cell Biology Research
Immunology and Inflammation Research
Metabolism Research
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
| Allele Symbol | Seletm1Hyn | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Richard O Hynes | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | E-selectin-; Sele-; | ||
| Mutation Made By | Richard Hynes, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Sele, selectin, endothelial cell | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | CD62E; E-selectin; ELAM; ELAM1; ESEL; Elam; LECAM2; endothelial adhesion molecule; | ||
| General Note | A modified D3 ES cell line heterozygous for a Selp mutation was used. | ||
| Molecular Note | The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal message in extracts from cardiac and pulmonary tissue of homozygous mutant mice. [MGI Ref ID J:31626] | ||
| Allele Symbol | Selltm1Hyn | ||
| Allele Name | targeted mutation 1, Richard Hynes | ||
| Allele Type | Targeted (knock-out) | ||
| Common Name(s) | L-Sel-; L-; | ||
| Mutation Made By | Richard Hynes, Massachusetts Institute of Technology | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Gene Symbol and Name | Sell, selectin, lymphocyte | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | A.11; AI528707; CD62L; L-selectin; LAM-1; LAM1; LECAM-1; LECAM1; LNHR; LSEL; LYAM1; Leu-8; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1; PLNHR; TQ1; expressed sequence AI528707; hLHRc; lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; lymphocyte antigen m22; | ||
| Molecular Note | A PGK-puro cassette was used to delete a 2.1kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of encoded protein in leukocytes isolated from homozygous mutant mice. Northern blot and Western blot analysis showed the transcript and protein levels of endothelial and platelet selectins to be unaffected by the targeting event. [MGI Ref ID J:57973] | ||
Genotyping Protocols
Seletm1Hyn, STD PCR, vers. 1
Selltm1Hyn, STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
Seletm1Hyn relatedSelltm1Hyn relatedBelanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798] [MGI Ref ID J:107461]
Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812] [MGI Ref ID J:137752]
Frenette J; Chbinou N; Godbout C; Marsolais D; Frenette PS. 2003. Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading. Am J Physiol Regul Integr Comp Physiol 285(4):R727-32. [PubMed: 12829442] [MGI Ref ID J:109393]
Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043] [MGI Ref ID J:31626]
Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58. [PubMed: 17456770] [MGI Ref ID J:121083]
Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587] [MGI Ref ID J:131154]
Ginhoux F; Collin MP; Bogunovic M; Abel M; Leboeuf M; Helft J; Ochando J; Kissenpfennig A; Malissen B; Grisotto M; Snoeck H; Randolph G; Merad M. 2007. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204(13):3133-46. [PubMed: 18086862] [MGI Ref ID J:130817]
Hidalgo A; Peired AJ; Wild MK; Vestweber D; Frenette PS. 2007. Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4):477-89. [PubMed: 17442598] [MGI Ref ID J:123577]
Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362] [MGI Ref ID J:107382]
Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773] [MGI Ref ID J:50212]
Horikawa M; Fujimoto M; Hasegawa M; Matsushita T; Hamaguchi Y; Kawasuji A; Matsushita Y; Fujita T; Ogawa F; Takehara K; Steeber DA; Sato S. 2006. E- and P-selectins synergistically inhibit bleomycin-induced pulmonary fibrosis. Am J Pathol 169(3):740-9. [PubMed: 16936251] [MGI Ref ID J:112363]
Kaifi JT; Hall LR; Diaz C; Sypek J; Diaconu E; Lass JH; Pearlman E. 2000. Impaired eosinophil recruitment to the cornea in P-selectin-deficient mice in onchocerca volvulus keratitis (River blindness) Invest Ophthalmol Vis Sci 41(12):3856-61. [PubMed: 11053286] [MGI Ref ID J:65551]
Komura K; Hasegawa M; Hamaguchi Y; Saito E; Kaburagi Y; Yanaba K; Kawara S; Takehara K; Seki M; Steeber DA; Tedder TF; Sato S. 2003. Ultraviolet light exposure suppresses contact hypersensitivity by abrogating endothelial intercellular adhesion molecule-1 up-regulation at the elicitation site. J Immunol 171(6):2855-62. [PubMed: 12960307] [MGI Ref ID J:85377]
Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599] [MGI Ref ID J:134224]
Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037] [MGI Ref ID J:115624]
Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197] [MGI Ref ID J:57973]
Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555] [MGI Ref ID J:62722]
Tang T; Frenette PS; Hynes RO; Wagner DD; Mayadas TN. 1996. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest 97(11):2485-90. [PubMed: 8647940] [MGI Ref ID J:107411]
Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670] [MGI Ref ID J:88109]
Yanaba K; Kaburagi Y; Takehara K; Steeber DA; Tedder TF; Sato S. 2003. Relative contributions of selectins and intercellular adhesion molecule-1 to tissue injury induced by immune complex deposition. Am J Pathol 162(5):1463-73. [PubMed: 12707029] [MGI Ref ID J:113592]
Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948] [MGI Ref ID J:107364]
Angeli V; Ginhoux F; Llodra J; Quemeneur L; Frenette PS; Skobe M; Jessberger R; Merad M; Randolph GJ. 2006. B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilization. Immunity 24(2):203-15. [PubMed: 16473832] [MGI Ref ID J:113320]
Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798] [MGI Ref ID J:107461]
Chen S; Kawashima H; Lowe JB; Lanier LL; Fukuda M. 2005. Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment. J Exp Med 202(12):1679-89. [PubMed: 16352740] [MGI Ref ID J:118830]
Csencsits KL; Pascual DW. 2002. Absence of L-selectin delays mucosal B cell responses in nonintestinal effector tissues. J Immunol 169(10):5649-59. [PubMed: 12421944] [MGI Ref ID J:80056]
Csencsits KL; Walters N; Pascual DW. 2001. Cutting edge: dichotomy of homing receptor dependence by mucosal effector B cells: alpha(E) versus L-selectin. J Immunol 167(5):2441-5. [PubMed: 11509580] [MGI Ref ID J:93848]
Czarneski J; Berguer P; Bekinschtein P; Kim DC; Hakimpour P; Wagner N; Nepomnaschy I; Piazzon I; Ross SR. 2002. Neonatal infection with a milk-borne virus is independent of beta7 integrin- and L-selectin-expressing lymphocytes. Eur J Immunol 32(4):945-56. [PubMed: 11920560] [MGI Ref ID J:76001]
Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812] [MGI Ref ID J:137752]
Eriksson EE; Xie X; Werr J; Thoren P; Lindbom L. 2001. Importance of primary capture and L-selectin-dependent secondary capture in leukocyte accumulation in inflammation and atherosclerosis in vivo. J Exp Med 194(2):205-18. [PubMed: 11457895] [MGI Ref ID J:118726]
Fukuyama S; Hiroi T; Yokota Y; Rennert PD; Yanagita M; Kinoshita N; Terawaki S; Shikina T; Yamamoto M; Kurono Y; Kiyono H. 2002. Initiation of NALT organogenesis is independent of the IL-7R, LTbetaR, and NIK signaling pathways but requires the Id2 gene and CD3(-)CD4(+)CD45(+) cells. Immunity 17(1):31-40. [PubMed: 12150889] [MGI Ref ID J:78101]
Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587] [MGI Ref ID J:131154]
Gonda A; Gal I; Szanto S; Sarraj B; Glant TT; Hunyadi J; Mikecz K. 2005. CD44, but not l-selectin, is critically involved in leucocyte migration into the skin in a murine model of allergic dermatitis. Exp Dermatol 14(9):700-8. [PubMed: 16098130] [MGI Ref ID J:114317]
Ho J; Kurtz CC; Naganuma M; Ernst PB; Cominelli F; Rivera-Nieves J. 2008. A CD8+/CD103high T cell subset regulates TNF-mediated chronic murine ileitis. J Immunol 180(4):2573-80. [PubMed: 18250468] [MGI Ref ID J:131978]
Laubli H; Stevenson JL; Varki A; Varki NM; Borsig L. 2006. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res 66(3):1536-42. [PubMed: 16452210] [MGI Ref ID J:106671]
Lawson JA; Burns AR; Farhood A; Lynn Bajt M; Collins RG; Smith CW; Jaeschke H. 2000. Pathophysiologic importance of E- and L-selectin for neutrophil-induced liver injury during endotoxemia in mice. Hepatology 32(5):990-8. [PubMed: 11050049] [MGI Ref ID J:106550]
Lejtenyi D; Osmond DG; Miller SC. 2003. Natural killer cells and B lymphocytes in L-selectin and Mac-1/LFA-1 knockout mice: marker-dependent, but not cell lineage-dependent changes in the spleen and bone marrow. Immunobiology 207(2):129-35. [PubMed: 12675270] [MGI Ref ID J:102758]
Li O; Liu JQ; Zhang H; Zheng P; Liu Y; Bai XF. 2006. CD62L is required for the priming of encephalitogenic T cells but does not play a major role in the effector phase of experimental autoimmune encephalomyelitis. Scand J Immunol 64(2):117-24. [PubMed: 16867156] [MGI Ref ID J:135873]
Martin AP; Coronel EC; Sano G; Chen SC; Vassileva G; Canasto-Chibuque C; Sedgwick JD; Frenette PS; Lipp M; Furtado GC; Lira SA. 2004. A novel model for lymphocytic infiltration of the thyroid gland generated by transgenic expression of the CC chemokine CCL21. J Immunol 173(8):4791-8. [PubMed: 15470018] [MGI Ref ID J:93715]
Pascual DW; Wang X; Kochetkova I; Callis G; Riccardi C. 2008. The absence of lymphoid CD8+ dendritic cell maturation in L-selectin-/- respiratory compartment attenuates antiviral immunity. J Immunol 181(2):1345-56. [PubMed: 18606689] [MGI Ref ID J:137476]
Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197] [MGI Ref ID J:57973]
Sarraj B; Ludanyi K; Glant TT; Finnegan A; Mikecz K. 2006. Expression of CD44 and L-selectin in the innate immune system is required for severe joint inflammation in the proteoglycan-induced murine model of rheumatoid arthritis. J Immunol 177(3):1932-40. [PubMed: 16849507] [MGI Ref ID J:138358]
Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555] [MGI Ref ID J:62722]
Szanto S; Gal I; Gonda A; Glant TT; Mikecz K. 2004. Expression of L-selectin, but not CD44, is required for early neutrophil extravasation in antigen-induced arthritis. J Immunol 172(11):6723-34. [PubMed: 15153489] [MGI Ref ID J:90526]
Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670] [MGI Ref ID J:88109]
Wang L; Brown JR; Varki A; Esko JD. 2002. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest 110(1):127-36. [PubMed: 12093896] [MGI Ref ID J:112426]
Colony Maintenance
Breeding & Husbandry This strain originated on a B6;129S2 background and is maintained as a homozygote. The donating lab maintained these mice by avoiding brother-sister matings.
| Pricing for USA, Canada and Mexico shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
| Pricing for International shipping destinations |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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