Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Dr. Richard Hynes,   Massachusetts Institute of Technology

Description
Mice that are homozygous null for the Sele, Sell and Selp genes are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities at birth. As mice mature, however, they become susceptible to mucocutaneous infections that eventually lead to death. No Sele, Sell or Selp gene products (mRNA or protein) are detected. Leukocytes from these mice exhibit a deficiency in the ability to interact with, and roll along, the venular wall endothelium. This deficiency in the crucial first step of leukocyte recruitment to surrounding tissues in response to infection or injury contributes to an elevated leukocyte count in the peripheral blood. Delays in neutrophil and eosinophil recruitment to the peritoneum in response to thioglycollate and ragweed allergen, respectively, have been observed, specifically. These mice are suitable for use in research applications studying leukocyte homeostasis, infectious diseases and inflammation.

Development
Sequential mutation by homologous recombination and selection was utilized to replace the Selp, Sele and Sell genes with drug-resistance genes for neomycin, hygromycin and puromycin (respectively) in 129S2/SvPas-derived D3 embryonic stem (ES) cells. This approach was necessary because all three genes are too closely linked for multiply deficient animals to be generated by mating single-deficient animals. Correctly targeted ES cells were injected into C57BL/6 blastocysts and chimeric offspring obtained.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Related Strains

Strains carrying   Sele^tm1Hyn allele

008439	B6.129S2-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008437	B6.129S2-Seletm1Hyn Selptm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
008440	C.129S2(B6)-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J

View Strains carrying   Sele^tm1Hyn     (5 strains)

Strains carrying   Sell^tm1Hyn allele

008439	B6.129S2-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008440	C.129S2(B6)-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J

View Strains carrying   Sell^tm1Hyn     (2 strains)

Strains carrying   Selp^tm1Hyn allele

008439	B6.129S2-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008437	B6.129S2-Seletm1Hyn Selptm1Hyn/J
008441	B6.129S2-Selltm3Hyn Selptm1Hyn/J
008432	B6.129S2-Selptm1Hyn/J
003805	B6;129S-Selltm3Hyn Selptm1Hyn/J
002916	B6;129S2-Seletm1Hyn Selptm1Hyn/J
002217	B6;129S2-Selptm1Hyn/J
008440	C.129S2(B6)-Seletm1Hyn Selltm1Hyn Selptm1Hyn/J
008438	C.129S2(B6)-Seletm1Hyn Selptm1Hyn/J
008442	C.129S2(B6)-Selltm3Hyn Selptm1Hyn/J
008433	C.129S2(B6)-Selptm1Hyn/J

View Strains carrying   Selp^tm1Hyn     (11 strains)

Strains carrying other alleles of Sele

008238	129S-Seletm1Dmil/J
008434	B6.129S2-Seletm2Hyn/J
008236	B6.129S4-Seletm1Dmil/J
003806	B6;129S-Seletm2Hyn Selltm4Hyn/J
002915	B6;129S2-Seletm2Hyn/J
008435	C.129S2(B6)-Seletm2Hyn/J
008237	C.129S4-Seletm1Dmil/J

View Strains carrying other alleles of Sele     (7 strains)

Strains carrying other alleles of Sell

008436	B6.129S2-Selltm2Hyn/J
008441	B6.129S2-Selltm3Hyn Selptm1Hyn/J
003806	B6;129S-Seletm2Hyn Selltm4Hyn/J
003805	B6;129S-Selltm3Hyn Selptm1Hyn/J
002917	B6;129S2-Selltm2Hyn/J
008442	C.129S2(B6)-Selltm3Hyn Selptm1Hyn/J
003860	NOD.129P2(B6)-Selltm1Tft/1LtJ
004943	NOD.Cg-Selltm1Tft Itgb7tm1Cgn/LtJ

View Strains carrying other alleles of Sell     (8 strains)

Strains carrying other alleles of Selp

002285	B6.129S7-Icam1tm1Bay Selptm1Bay/J
002289	B6.129S7-Selptm1Bay/J

View Strains carrying other alleles of Selp     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Hypertension, Essential   (SELE) IgE Responsiveness, Atopic; IGER   (SELP) Selectin P; SELP   (SELP)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Sele^tm1Hyn/Sele^tm1Hyn Sell^tm1Hyn/Sell^tm1Hyn Selp^tm1Hyn/Selp^tm1Hyn

        involves: 129S2/SvPas * C57BL/6

• immune system phenotype
• abnormal leukocyte tethering or rolling
  • rolling leukocytes are virtually absent within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
  • there is a three-quarter decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
  • a population of leukocytes that are "fast rollers" is evident in these mice that are not observed in wild-type mice   (MGI Ref ID J:57973)
• impaired eosinophil recruitment
  • 2-fold fewer eosinophils are recruited to the peritoneum in response to ragweed allergen challenge   (MGI Ref ID J:57973)
• impaired neutrophil recruitment
  • neutrophil influx in thioglycollate-induced peritonitis is inhibited at 2, 4, and 8 hours after injection compared to wild-type mice   (MGI Ref ID J:57973)
  • lower numbers of neutrophils are observed than those elicited from mice that are null homozygotes for just one gene   (MGI Ref ID J:57973)
• increased leukocyte cell number
  • there is a 2.5 fold increase in leukocyte numbers   (MGI Ref ID J:57973)
• • increased eosinophil cell number
    • eosinophil numbers are about 6-fold higher in the blood   (MGI Ref ID J:57973)
  • increased monocyte cell number
    • monocyte number are increased about 5-fold   (MGI Ref ID J:57973)
  • increased neutrophil cell number
    • neutrophils numbers are increased almost 10-fold   (MGI Ref ID J:57973)
• hematopoietic system phenotype
• increased leukocyte cell number
  • there is a 2.5 fold increase in leukocyte numbers   (MGI Ref ID J:57973)
• • increased eosinophil cell number
    • eosinophil numbers are about 6-fold higher in the blood   (MGI Ref ID J:57973)
  • increased monocyte cell number
    • monocyte number are increased about 5-fold   (MGI Ref ID J:57973)
  • increased neutrophil cell number
    • neutrophils numbers are increased almost 10-fold   (MGI Ref ID J:57973)
• cellular phenotype
• abnormal leukocyte tethering or rolling
  • rolling leukocytes are virtually absent within the mesenteric venules of mice treated with TNF   (MGI Ref ID J:57973)
  • there is a three-quarter decrease in the number of adhering leukocytes within these mesenteric venules   (MGI Ref ID J:57973)
  • a population of leukocytes that are "fast rollers" is evident in these mice that are not observed in wild-type mice   (MGI Ref ID J:57973)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Sele^tm1Hyn related

Cardiovascular Research
Atherosclerosis
Vascular Defects
      defective leukocyte function

Cell Biology Research
Defects in Cell Adhesion Molecules

Developmental Biology Research
Defects in Cell Adhesion Molecules

Hematological Research
Hematopoietic Defects
Immunological Defects

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency Associated with Other Defects
Inflammation

Research Tools
Cell Biology Research
Immunology and Inflammation Research
Metabolism Research

Sell^tm1Hyn related

Immunology, Inflammation and Autoimmunity Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Selp^tm1Hyn related

Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Seletm1Hyn
Allele Name		targeted mutation 1, Richard Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		E-selectin-; Sele-;
Mutation Made By		Dr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Sele, selectin, endothelial cell
Chromosome		1
Gene Common Name(s)		CD62E; E-selectin; ELAM; ELAM1; ESEL; Elam; LECAM2; endothelial adhesion molecule;
Molecular Note		A modified D3 ES cell line heterozygous for the Selptm1Hyn allele was targeted a second time. The exons encoding the signal peptide, lectin domain, and a portion of the epidermal growth factor domain were replaced by the insertion of a PGK-hygro cassette. Both Northern and RT-PCR analysis revealed an absence of normal message for both genes in extracts from cardiac and pulmonary tissue of homozygous mutant mice. This targeted mutation occurred in cis with the Selptm1Hyn mutation. Due to their genomic proximity, the two null alleles should propagate together through the offspring. [MGI Ref ID J:31626]
Allele Symbol		Selltm1Hyn
Allele Name		targeted mutation 1, Richard Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		L-Sel-; L-;
Mutation Made By		Dr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Sell, selectin, lymphocyte
Chromosome		1
Gene Common Name(s)		A.11; AI528707; CD62L; L-selectin; LAM1; LECAM-1; LECAM1; LEU8; LNHR; LSEL; LYAM1; Lnhr; Ly-22; Ly-m22; Lyam-1; Lyam1; PLNHR; TQ1; expressed sequence AI528707; lymph node homing receptor; lymphocyte adhesion molecule 1; lymphocyte antigen 22; lymphocyte antigen m22;
Molecular Note		The three selectin genes, Sele, Sell, and Selp, are found within a 300 kb genomic region. In order to create a triple knockout, ES cells heterozygote for the Selptm1Hyn and Seletm1Hyn null alleles in cis to each other were targeteda third time. A PGK-puro cassette was used to delete a 2.1kb region containing the exons encoding the lectin domain and most of the epidermal growth factor domain. Flow cytometric analysis showed an absence of all three encoded proteins in leukocytes isolated from homozygotes. This targeted mutation occurred in cis with the other two null mutations. Due to their genomic proximity, the three null alleles should propagate together through the offspring. [MGI Ref ID J:57973]
Allele Symbol		Selptm1Hyn
Allele Name		targeted mutation 1, Richard Hynes
Allele Type		Targeted (knock-out)
Common Name(s)		P-; P-Selp-; P-selectin-;
Mutation Made By		Dr. Richard Hynes,   Massachusetts Institute of Technology
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Selp, selectin, platelet
Chromosome		1
Gene Common Name(s)		CD62; CD62P; GMP140; GRMP; Grmp; LECAM3; P-selectin; PADGEM; PSEL; PSELECT; granulocyte membrane protein;
Molecular Note		A portion of exon 3 encoding 10 amino acids of a signal peptide and 27 amino acids of the lectin domain was replaced with a PGK-neo cassette. Mice were treated with lipopolysaccharide to augment the normally low levels of selectin in lung and liver tissue. A longer transcript, resulting from aberrant or cryptic splicing involving the neomycin cassette, was detected at low levels in mutant mice by Northern blot analysis. RT-PCR further confirmed the presence of low levels of transcript in homozygous mice.Immunofluorescence analysis of activated platelets and lung sections from homozygous mutant mice showed an absence of encoded protein. These results were further confirmed by flow cytometric analysis of activated platelets and metabolic labeling of lung tissue. [MGI Ref ID J:77329]

Genotyping

Genotyping Information

Genotyping Protocols

Sele^tm1Hyn, Standard PCR
Sell^tm1Hyn, Standard PCR
Selp^tm1Hyn, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Sele^tm1Hyn related

Doring A; Wild M; Vestweber D; Deutsch U; Engelhardt B. 2007. E- and P-selectin are not required for the development of experimental autoimmune encephalomyelitis in C57BL/6 and SJL mice. J Immunol 179(12):8470-9. [PubMed: 18056394]  [MGI Ref ID J:155033]

Frenette J; Chbinou N; Godbout C; Marsolais D; Frenette PS. 2003. Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading. Am J Physiol Regul Integr Comp Physiol 285(4):R727-32. [PubMed: 12829442]  [MGI Ref ID J:109393]

Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043]  [MGI Ref ID J:31626]

Fujita T; Fujimoto M; Matsushita T; Shimada Y; Hasegawa M; Kuwano Y; Ogawa F; Takehara K; Sato S. 2007. Phase-Dependent Roles of E-Selectin during Chronic Contact Hypersensitivity Responses. Am J Pathol 170(5):1649-58. [PubMed: 17456770]  [MGI Ref ID J:121083]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Ginhoux F; Collin MP; Bogunovic M; Abel M; Leboeuf M; Helft J; Ochando J; Kissenpfennig A; Malissen B; Grisotto M; Snoeck H; Randolph G; Merad M. 2007. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204(13):3133-46. [PubMed: 18086862]  [MGI Ref ID J:130817]

Hidalgo A; Chang J; Jang JE; Peired AJ; Chiang EY; Frenette PS. 2009. Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury. Nat Med 15(4):384-91. [PubMed: 19305412]  [MGI Ref ID J:149370]

Hidalgo A; Peired AJ; Wild MK; Vestweber D; Frenette PS. 2007. Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44. Immunity 26(4):477-89. [PubMed: 17442598]  [MGI Ref ID J:123577]

Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362]  [MGI Ref ID J:107382]

Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773]  [MGI Ref ID J:50212]

Ishii T; Fujita T; Matsushita T; Yanaba K; Hasegawa M; Nakashima H; Ogawa F; Shimizu K; Takehara K; Tedder TF; Sato S; Fujimoto M. 2009. Establishment of experimental eosinophilic vasculitis by IgE-mediated cutaneous reverse passive arthus reaction. Am J Pathol 174(6):2225-33. [PubMed: 19389931]  [MGI Ref ID J:148778]

Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599]  [MGI Ref ID J:134224]

Nacher M; Blazquez AB; Shao B; Matesanz A; Prophete C; Berin MC; Frenette PS; Hidalgo A. 2011. Physiological Contribution of CD44 as a Ligand for E-Selectin during Inflammatory T-Cell Recruitment. Am J Pathol 178(5):2437-46. [PubMed: 21457936]  [MGI Ref ID J:171380]

Oh IY; Yoon CH; Hur J; Kim JH; Kim TY; Lee CS; Park KW; Chae IH; Oh BH; Park YB; Kim HS. 2007. Involvement of E-selectin in recruitment of endothelial progenitor cells and angiogenesis in ischemic muscle. Blood 110(12):3891-9. [PubMed: 17699745]  [MGI Ref ID J:149107]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Scheiermann C; Kunisaki Y; Lucas D; Chow A; Jang JE; Zhang D; Hashimoto D; Merad M; Frenette PS. 2012. Adrenergic nerves govern circadian leukocyte recruitment to tissues. Immunity 37(2):290-301. [PubMed: 22863835]  [MGI Ref ID J:187383]

Sobolev O; Stern P; Lacy-Hulbert A; Hynes RO. 2009. Natural killer cells require selectins for suppression of subcutaneous tumors. Cancer Res 69(6):2531-9. [PubMed: 19258505]  [MGI Ref ID J:146885]

Stubke K; Wicklein D; Herich L; Schumacher U; Nehmann N. 2012. Selectin-deficiency reduces the number of spontaneous metastases in a xenograft model of human breast cancer. Cancer Lett 321(1):89-99. [PubMed: 22366582]  [MGI Ref ID J:185633]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Tang T; Frenette PS; Hynes RO; Wagner DD; Mayadas TN. 1996. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest 97(11):2485-90. [PubMed: 8647940]  [MGI Ref ID J:107411]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Yago T; Fu J; McDaniel JM; Miner JJ; McEver RP; Xia L. 2010. Core 1-derived O-glycans are essential E-selectin ligands on neutrophils. Proc Natl Acad Sci U S A 107(20):9204-9. [PubMed: 20439727]  [MGI Ref ID J:160571]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Sell^tm1Hyn related

Angeli V; Ginhoux F; Llodra J; Quemeneur L; Frenette PS; Skobe M; Jessberger R; Merad M; Randolph GJ. 2006. B cell-driven lymphangiogenesis in inflamed lymph nodes enhances dendritic cell mobilization. Immunity 24(2):203-15. [PubMed: 16473832]  [MGI Ref ID J:113320]

Carvalho LP; Petritus PM; Trochtenberg AL; Zaph C; Hill DA; Artis D; Scott P. 2012. Lymph node hypertrophy following Leishmania major infection is dependent on TLR9. J Immunol 188(3):1394-401. [PubMed: 22205030]  [MGI Ref ID J:180754]

Chen S; Kawashima H; Lowe JB; Lanier LL; Fukuda M. 2005. Suppression of tumor formation in lymph nodes by L-selectin-mediated natural killer cell recruitment. J Exp Med 202(12):1679-89. [PubMed: 16352740]  [MGI Ref ID J:118830]

Collins CB; Ho J; Wilson TE; Wermers JD; Tlaxca JL; Lawrence MB; Solga M; Lannigan J; Rivera-Nieves J. 2008. CD44 deficiency attenuates chronic murine ileitis. Gastroenterology 135(6):1993-2002. [PubMed: 18854186]  [MGI Ref ID J:145626]

Eriksson EE. 2008. No detectable endothelial- or leukocyte-derived L-selectin ligand activity on the endothelium in inflamed cremaster muscle venules. J Leukoc Biol 84(1):93-103. [PubMed: 18381812]  [MGI Ref ID J:137752]

Eriksson EE; Xie X; Werr J; Thoren P; Lindbom L. 2001. Importance of primary capture and L-selectin-dependent secondary capture in leukocyte accumulation in inflammation and atherosclerosis in vivo. J Exp Med 194(2):205-18. [PubMed: 11457895]  [MGI Ref ID J:118726]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Ho J; Kurtz CC; Naganuma M; Ernst PB; Cominelli F; Rivera-Nieves J. 2008. A CD8+/CD103high T cell subset regulates TNF-mediated chronic murine ileitis. J Immunol 180(4):2573-80. [PubMed: 18250468]  [MGI Ref ID J:131978]

Laubli H; Stevenson JL; Varki A; Varki NM; Borsig L. 2006. L-selectin facilitation of metastasis involves temporal induction of Fut7-dependent ligands at sites of tumor cell arrest. Cancer Res 66(3):1536-42. [PubMed: 16452210]  [MGI Ref ID J:106671]

Lawson JA; Burns AR; Farhood A; Lynn Bajt M; Collins RG; Smith CW; Jaeschke H. 2000. Pathophysiologic importance of E- and L-selectin for neutrophil-induced liver injury during endotoxemia in mice. Hepatology 32(5):990-8. [PubMed: 11050049]  [MGI Ref ID J:106550]

Li O; Liu JQ; Zhang H; Zheng P; Liu Y; Bai XF. 2006. CD62L is required for the priming of encephalitogenic T cells but does not play a major role in the effector phase of experimental autoimmune encephalomyelitis. Scand J Immunol 64(2):117-24. [PubMed: 16867156]  [MGI Ref ID J:135873]

Ludwig RJ; Bergmann P; Garbaraviciene J; von Stebut E; Radeke HH; Gille J; Diehl S; Hardt K; Henschler R; Kaufmann R; Pfeilschifter JM; Boehncke WH. 2010. Platelet, not endothelial, p-selectin expression contributes to generation of immunity in cutaneous contact hypersensitivity. Am J Pathol 176(3):1339-45. [PubMed: 20056837]  [MGI Ref ID J:158387]

Pascual DW; Wang X; Kochetkova I; Callis G; Riccardi C. 2008. The absence of lymphoid CD8+ dendritic cell maturation in L-selectin-/- respiratory compartment attenuates antiviral immunity. J Immunol 181(2):1345-56. [PubMed: 18606689]  [MGI Ref ID J:137476]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Sarraj B; Ludanyi K; Glant TT; Finnegan A; Mikecz K. 2006. Expression of CD44 and L-selectin in the innate immune system is required for severe joint inflammation in the proteoglycan-induced murine model of rheumatoid arthritis. J Immunol 177(3):1932-40. [PubMed: 16849507]  [MGI Ref ID J:138358]

Sobolev O; Stern P; Lacy-Hulbert A; Hynes RO. 2009. Natural killer cells require selectins for suppression of subcutaneous tumors. Cancer Res 69(6):2531-9. [PubMed: 19258505]  [MGI Ref ID J:146885]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Wang L; Brown JR; Varki A; Esko JD. 2002. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest 110(1):127-36. [PubMed: 12093896]  [MGI Ref ID J:112426]

Yago T; Fu J; McDaniel JM; Miner JJ; McEver RP; Xia L. 2010. Core 1-derived O-glycans are essential E-selectin ligands on neutrophils. Proc Natl Acad Sci U S A 107(20):9204-9. [PubMed: 20439727]  [MGI Ref ID J:160571]

Zhang N; Schroppel B; Lal G; Jakubzick C; Mao X; Chen D; Yin N; Jessberger R; Ochando JC; Ding Y; Bromberg JS. 2009. Regulatory T cells sequentially migrate from inflamed tissues to draining lymph nodes to suppress the alloimmune response. Immunity 30(3):458-69. [PubMed: 19303390]  [MGI Ref ID J:147032]

Selp^tm1Hyn related

Andre P; Denis CV; Ware J; Saffaripour S; Hynes RO; Ruggeri ZM; Wagner DD. 2000. Platelets adhere to and translocate on von willebrand factor presented by endothelium in stimulated veins Blood 96(10):3322-8. [PubMed: 11071623]  [MGI Ref ID J:65812]

Andre P; Hartwell D; Hrachovinova I; Saffaripour S; Wagner DD. 2000. Pro-coagulant state resulting from high levels of soluble P-selectin in blood. Proc Natl Acad Sci U S A 97(25):13835-40. [PubMed: 11095738]  [MGI Ref ID J:125020]

Belanger SD; St-Pierre Y. 2005. Role of selectins in the triggering, growth, and dissemination of T-lymphoma cells: implication of L-selectin in the growth of thymic lymphoma. Blood 105(12):4800-6. [PubMed: 15705798]  [MGI Ref ID J:107461]

Combes V; Rosenkranz AR; Redard M; Pizzolato G; Lepidi H; Vestweber D; Mayadas TN; Grau GE. 2004. Pathogenic role of P-selectin in experimental cerebral malaria: importance of the endothelial compartment. Am J Pathol 164(3):781-6. [PubMed: 14982832]  [MGI Ref ID J:88446]

Connolly ES Jr; Winfree CJ; Prestigiacomo CJ; Kim SC; Choudhri TF; Hoh BL; Naka Y; Solomon RA; Pinsky DJ. 1997. Exacerbation of cerebral injury in mice that express the P-selectin gene: identification of P-selectin blockade as a new target for the treatment of stroke. Circ Res 81(3):304-10. [PubMed: 9285631]  [MGI Ref ID J:43110]

Dole VS; Bergmeier W; Mitchell HA; Eichenberger SC; Wagner DD. 2005. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin. Blood 106(7):2334-9. [PubMed: 15956287]  [MGI Ref ID J:119376]

Doring A; Wild M; Vestweber D; Deutsch U; Engelhardt B. 2007. E- and P-selectin are not required for the development of experimental autoimmune encephalomyelitis in C57BL/6 and SJL mice. J Immunol 179(12):8470-9. [PubMed: 18056394]  [MGI Ref ID J:155033]

Egami K; Murohara T; Aoki M; Matsuishi T. 2006. Ischemia-induced angiogenesis: role of inflammatory response mediated by P-selectin. J Leukoc Biol 79(5):971-6. [PubMed: 16641139]  [MGI Ref ID J:108660]

Fernekorn U; Butcher EC; Behrends J; Hartz S; Kruse A. 2004. Functional involvement of P-selectin and MAdCAM-1 in the recruitment of alpha4beta7-integrin-expressing monocyte-like cells to the pregnant mouse uterus. Eur J Immunol 34(12):3423-33. [PubMed: 15484189]  [MGI Ref ID J:94597]

Fernekorn U; Butcher EC; Behrends J; Karsten CM; Robke A; Schulze TJ; Kirchner H; Kruse A. 2007. Selectin, platelet plays a critical role in granulocyte access to the pregnant mouse uterus under physiological and pathological conditions. Biol Reprod 76(4):645-53. [PubMed: 17151351]  [MGI Ref ID J:121141]

Forlow SB; White EJ; Barlow SC; Feldman SH; Lu H; Bagby GJ; Beaudet AL; Bullard DC; Ley K. 2000. Severe inflammatory defect and reduced viability in CD18 and E-selectin double-mutant mice J Clin Invest 106(12):1457-66. [PubMed: 11120753]  [MGI Ref ID J:66426]

Frederix K; Chauhan AK; Kisucka J; Zhao BQ; Hoff EI; Spronk HM; Ten Cate H; Wagner DD. 2007. Platelet adhesion receptors do not modulate infarct volume after a photochemically induced stroke in mice. Brain Res 1185:239-45. [PubMed: 17996853]  [MGI Ref ID J:130124]

Frenette J; Chbinou N; Godbout C; Marsolais D; Frenette PS. 2003. Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading. Am J Physiol Regul Integr Comp Physiol 285(4):R727-32. [PubMed: 12829442]  [MGI Ref ID J:109393]

Frenette PS; Mayadas TN; Rayburn H; Hynes RO; Wagner DD. 1996. Susceptibility to infection and altered hematopoiesis in mice deficient in both P- and E-selectins. Cell 84(4):563-74. [PubMed: 8598043]  [MGI Ref ID J:31626]

Ghosh S; Chackerian AA; Parker CM; Ballantyne CM; Behar SM. 2006. The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection. J Immunol 176(8):4914-22. [PubMed: 16585587]  [MGI Ref ID J:131154]

Ginhoux F; Collin MP; Bogunovic M; Abel M; Leboeuf M; Helft J; Ochando J; Kissenpfennig A; Malissen B; Grisotto M; Snoeck H; Randolph G; Merad M. 2007. Blood-derived dermal langerin+ dendritic cells survey the skin in the steady state. J Exp Med 204(13):3133-46. [PubMed: 18086862]  [MGI Ref ID J:130817]

Gironella M; Molla M; Salas A; Soriano A; Sans M; Closa D; Engel P; Salas A; Pique JM; Panes J. 2002. The role of P-selectin in experimental colitis as determined by antibody immunoblockade and genetically deficient mice. J Leukoc Biol 72(1):56-64. [PubMed: 12101263]  [MGI Ref ID J:124457]

Goerge T; Ho-Tin-Noe B; Carbo C; Benarafa C; Remold-O'Donnell E; Zhao BQ; Cifuni SM; Wagner DD. 2008. Inflammation induces hemorrhage in thrombocytopenia. Blood 111(10):4958-64. [PubMed: 18256319]  [MGI Ref ID J:135316]

Hara T; Shimizu K; Ogawa F; Yanaba K; Iwata Y; Muroi E; Takenaka M; Komura K; Hasegawa M; Fujimoto M; Sato S. 2010. Platelets control leukocyte recruitment in a murine model of cutaneous arthus reaction. Am J Pathol 176(1):259-69. [PubMed: 20008131]  [MGI Ref ID J:156487]

Hidalgo A; Chang J; Jang JE; Peired AJ; Chiang EY; Frenette PS. 2009. Heterotypic interactions enabled by polarized neutrophil microdomains mediate thromboinflammatory injury. Nat Med 15(4):384-91. [PubMed: 19305412]  [MGI Ref ID J:149370]

Hirata T; Furie BC; Furie B. 2002. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 169(8):4307-13. [PubMed: 12370362]  [MGI Ref ID J:107382]

Homeister JW; Zhang M; Frenette PS; Hynes RO; Wagner DD; Lowe JB; Marks RM. 1998. Overlapping functions of E- and P-selectin in neutrophil recruitment during acute inflammation. Blood 92(7):2345-52. [PubMed: 9746773]  [MGI Ref ID J:50212]

Horie Y; Wolf R; Anderson DC; Granger DN. 1997. Hepatic leukostasis and hypoxic stress in adhesion molecule-deficient mice after gut ischemia/reperfusion. J Clin Invest 99(4):781-8. [PubMed: 9045883]  [MGI Ref ID J:39161]

Huang HS; Sun DS; Lien TS; Chang HH. 2010. Dendritic cells modulate platelet activity in IVIg-mediated amelioration of ITP in mice. Blood 116(23):5002-9. [PubMed: 20699442]  [MGI Ref ID J:167263]

Johnson RC; Mayadas TN; Frenette PS; Mebius RE; Subramaniam M; Lacasce A; Hynes RO; Wagner DD. 1995. Blood cell dynamics in P-selectin-deficient mice. Blood 86(3):1106-14. [PubMed: 7542495]  [MGI Ref ID J:28190]

Kaifi JT; Hall LR; Diaz C; Sypek J; Diaconu E; Lass JH; Pearlman E. 2000. Impaired eosinophil recruitment to the cornea in P-selectin-deficient mice in onchocerca volvulus keratitis (River blindness) Invest Ophthalmol Vis Sci 41(12):3856-61. [PubMed: 11053286]  [MGI Ref ID J:65551]

Karsten CM; Behrends J; Wagner AK; Fuchs F; Figge J; Schmudde I; Hellberg L; Kruse A. 2009. DC within the pregnant mouse uterus influence growth and functional properties of uterine NK cells. Eur J Immunol 39(8):2203-14. [PubMed: 19593769]  [MGI Ref ID J:151694]

Lu SX; Holland AM; Na IK; Terwey TH; Alpdogan O; Bautista JL; Smith OM; Suh D; King C; Kochman A; Hubbard VM; Rao UK; Yim N; Liu C; Laga AC; Murphy G; Jenq RR; Zakrzewski JL; Penack O; Dykstra L; Bampoe K; Perez L; Furie B; Furie B; van den Brink MR. 2010. Absence of P-selectin in recipients of allogeneic bone marrow transplantation ameliorates experimental graft-versus-host disease. J Immunol 185(3):1912-9. [PubMed: 20622117]  [MGI Ref ID J:162237]

Massaguer A; Perez-Del-Pulgar S; Engel P; Serratosa J; Bosch J; Pizcueta P. 2002. Concanavalin-A-induced liver injury is severely impaired in mice deficient in P-selectin. J Leukoc Biol 72(2):262-70. [PubMed: 12149416]  [MGI Ref ID J:124455]

Mayadas TN. 1995. Gene knockout on p-selectin: its biology and function. Trends Cardiovasc Med 5(4):149-157.  [MGI Ref ID J:28867]

Mayadas TN; Johnson RC; Rayburn H; Hynes RO; Wagner DD. 1993. Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell 74(3):541-54. [PubMed: 7688665]  [MGI Ref ID J:77329]

Mayadas TN; Mendrick DL; Brady HR; Tang T; Papayianni A; Assmann KJ; Wagner DD; Hynes RO; Cotran RS. 1996. Acute passive anti-glomerular basement membrane nephritis in P-selectin-deficient mice. Kidney Int 49(5):1342-9. [PubMed: 8731099]  [MGI Ref ID J:113194]

McCafferty DM; Kanwar S; Granger DN; Kubes P. 2000. E/P-selectin-deficient mice: an optimal mutation for abrogating antigen but not tumor necrosis factor-alpha-induced immune responses Eur J Immunol 30(8):2362-71. [PubMed: 10940927]  [MGI Ref ID J:63951]

McCafferty DM; Smith CW; Granger DN; Kubes P. 1999. Intestinal inflammation in adhesion molecule-deficient mice: an assessment of P-selectin alone and in combination with ICAM-1 or E-selectin. J Leukoc Biol 66(1):67-74. [PubMed: 10410991]  [MGI Ref ID J:56590]

Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599]  [MGI Ref ID J:134224]

Nacher M; Blazquez AB; Shao B; Matesanz A; Prophete C; Berin MC; Frenette PS; Hidalgo A. 2011. Physiological Contribution of CD44 as a Ligand for E-Selectin during Inflammatory T-Cell Recruitment. Am J Pathol 178(5):2437-46. [PubMed: 21457936]  [MGI Ref ID J:171380]

Papayannopoulou T; Priestley GV; Nakamoto B; Zafiropoulos V; Scott LM. 2001. Molecular pathways in bone marrow homing: dominant role of alpha(4)beta(1) over beta(2)-integrins and selectins. Blood 98(8):2403-11. [PubMed: 11588037]  [MGI Ref ID J:115624]

Patrick AL; Rullo J; Beaudin S; Liaw P; Fox-Robichaud AE. 2007. Hepatic leukocyte recruitment in response to time-limited expression of TNF-alpha and IL-1beta. Am J Physiol Gastrointest Liver Physiol 293(4):G663-72. [PubMed: 17656447]  [MGI Ref ID J:126664]

Planck SR; Han YB; Park JM; O'Rourke L; Gutierrez-Ramos JC; Rosenbaum JT. 1998. The effect of genetic deficiency of adhesion molecules on the course of endotoxin-induced uveitis. Curr Eye Res 17(9):941-6. [PubMed: 9746442]  [MGI Ref ID J:114206]

Robinson SD; Frenette PS; Rayburn H; Cummiskey M; Ullman-Cullere M; Wagner DD; Hynes RO. 1999. Multiple, targeted deficiencies in selectins reveal a predominant role for P-selectin in leukocyte recruitment. Proc Natl Acad Sci U S A 96(20):11452-7. [PubMed: 10500197]  [MGI Ref ID J:57973]

Scheiermann C; Kunisaki Y; Lucas D; Chow A; Jang JE; Zhang D; Hashimoto D; Merad M; Frenette PS. 2012. Adrenergic nerves govern circadian leukocyte recruitment to tissues. Immunity 37(2):290-301. [PubMed: 22863835]  [MGI Ref ID J:187383]

Singh I; Zibari GB; Brown MF; Granger DN; Eppihimer M; Zizzi H; Cruz L; Meyer K; Gonzales E; McDonald JC. 1999. Role of P-selectin expression in hepatic ischemia and reperfusion injury. Clin Transplant 13(1 Pt 2):76-82. [PubMed: 10081641]  [MGI Ref ID J:103151]

Sobolev O; Stern P; Lacy-Hulbert A; Hynes RO. 2009. Natural killer cells require selectins for suppression of subcutaneous tumors. Cancer Res 69(6):2531-9. [PubMed: 19258505]  [MGI Ref ID J:146885]

Stokol T; O'Donnell P; Xiao L; Knight S; Stavrakis G; Botto M; von Andrian UH; Mayadas TN. 2004. C1q governs deposition of circulating immune complexes and leukocyte Fcgamma receptors mediate subsequent neutrophil recruitment. J Exp Med 200(7):835-46. [PubMed: 15466618]  [MGI Ref ID J:93949]

Stubke K; Wicklein D; Herich L; Schumacher U; Nehmann N. 2012. Selectin-deficiency reduces the number of spontaneous metastases in a xenograft model of human breast cancer. Cancer Lett 321(1):89-99. [PubMed: 22366582]  [MGI Ref ID J:185633]

Subramaniam M; Frenette PS; Saffaripour S; Johnson RC; Hynes RO; Wagner DD. 1996. Defects in hemostasis in P-selectin-deficient mice. Blood 87(4):1238-42. [PubMed: 8608210]  [MGI Ref ID J:31431]

Subramaniam M; Saffaripour S; Watson SR; Mayadas TN; Hynes RO; Wagner DD. 1995. Reduced recruitment of inflammatory cells in a contact hypersensitivity response in P-selectin-deficient mice. J Exp Med 181(6):2277-82. [PubMed: 7539046]  [MGI Ref ID J:110854]

Sweeney EA; Priestley GV; Nakamoto B; Collins RG; Beaudet AL; Papayannopoulou T. 2000. Mobilization of stem/progenitor cells by sulfated polysaccharides does not require selectin presence. Proc Natl Acad Sci U S A 97(12):6544-9. [PubMed: 10841555]  [MGI Ref ID J:62722]

Tang T; Frenette PS; Hynes RO; Wagner DD; Mayadas TN. 1996. Cytokine-induced meningitis is dramatically attenuated in mice deficient in endothelial selectins. J Clin Invest 97(11):2485-90. [PubMed: 8647940]  [MGI Ref ID J:107411]

Taverna D; Moher H; Crowley D; Borsig L; Varki A; Hynes RO. 2004. Increased primary tumor growth in mice null for beta3- or beta3/beta5-integrins or selectins. Proc Natl Acad Sci U S A 101(3):763-8. [PubMed: 14718670]  [MGI Ref ID J:88109]

Tomita H; Iwata Y; Ogawa F; Komura K; Shimizu K; Yoshizaki A; Hara T; Muroi E; Yanaba K; Bae S; Takenaka M; Hasegawa M; Fujimoto M; Sato S. 2009. P-selectin glycoprotein ligand-1 contributes to wound healing predominantly as a p-selectin ligand and partly as an e-selectin ligand. J Invest Dermatol 129(8):2059-67. [PubMed: 19177138]  [MGI Ref ID J:152512]

Tu L; Poe JC; Kadono T; Venturi GM; Bullard DC; Tedder TF; Steeber DA. 2002. A functional role for circulating mouse L-selectin in regulating leukocyte/endothelial cell interactions in vivo. J Immunol 169(4):2034-43. [PubMed: 12165530]  [MGI Ref ID J:120206]

Wang L; Brown JR; Varki A; Esko JD. 2002. Heparin's anti-inflammatory effects require glucosamine 6-O-sulfation and are mediated by blockade of L- and P-selectins. J Clin Invest 110(1):127-36. [PubMed: 12093896]  [MGI Ref ID J:112426]

Yago T; Fu J; McDaniel JM; Miner JJ; McEver RP; Xia L. 2010. Core 1-derived O-glycans are essential E-selectin ligands on neutrophils. Proc Natl Acad Sci U S A 107(20):9204-9. [PubMed: 20439727]  [MGI Ref ID J:160571]

Yamada S; Mayadas TN; Yuan F; Wagner DD; Hynes RO; Melder RJ; Jain RK. 1995. Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin. Blood 86(9):3487-92. [PubMed: 7579454]  [MGI Ref ID J:29814]

Zaph C; Scott P. 2003. Th1 cell-mediated resistance to cutaneous infection with Leishmania major is independent of P- and E-selectins. J Immunol 171(9):4726-32. [PubMed: 14568948]  [MGI Ref ID J:107364]

Zhang S; Condac E; Qiu H; Jiang J; Gutierrez-Sanchez G; Bergmann C; Handel T; Wang L. 2012. Heparin-induced leukocytosis requires 6-O-sulfation and is caused by blockade of selectin- and CXCL12 protein-mediated leukocyte trafficking in mice. J Biol Chem 287(8):5542-53. [PubMed: 22194593]  [MGI Ref ID J:182444]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

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Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

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Breeding & Husbandry	This strain originated on a mixed B6;129S2 background and is maintained as a homozygote. The donating investigator maintained this strain avoiding brother-sister matings. Coat color expected from breeding:Black and Agouti
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