Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse   Donating Investigator Brian Kobilka,   Stanford University

Description
Mice that are homozygous null for the Adrb1 and Adrb2 genes are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Stimulation of beta adrenergic receptor function in these mice by agonists or exercise reveals significant impairments in chronotropic range, vascular reactivity and metabolic rate. A severely attenuated chronotropic and hypotensive response is observed after administration of the non-selective beta adrenergic receptor agonist isoproterenol. An abnormal response to epinephrine is also seen, with bradycardia and a monophasic hypertensive blood pressure change being observed rather than the tachycardia and biphasic hypertensive/ hypotensive response seen in wildtype mice. When exercised, heart rates in null mice are lower than that of wild type mice. No difference is noted in the resting heart rate.

Development
Double knockout mice were created by mating Adrb1 homozygous knockout mice with Adrb2 homozygous knockout mice to generate compound heterozygotes, the offspring of which were then mated to obtain compound homozygotes. Adrb1 null mice were created using a targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter to disrupt most of the Adrb1coding region (all but a 3' 153 bp). The construct was transfected into 129- derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric male animals were mated to C57BL/6J X DBA/2 F1 hybrids. Adrb2 null mice were created in a similar fashion using a targeting vector again containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter to disrupt Adrb2 such that the end of the fourth transmembrane segment is absent, rendering the receptor nonfunctional. The construct was transfected into 129- derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into CD-1 blastocysts. The resulting chimeric male animals were mated to FVB/N females.

Control Information

	Control
	None Available
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Adrb1^tm1Bkk/Adrb1^tm1Bkk Adrb2^tm1Bkk/Adrb2^tm1Bkk

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2 * FVB/N

• cardiovascular system phenotype
• abnormal cardiovascular system physiology (MGI Ref ID J:55553)
  • abnormal responses to pharmacological stimuli; attenuation of both the normal tachycardia and hypotensive responses to isoproterenol administration and bradycardia and monophasic hypertension in response to epinephrine administration
• decreased cardiac muscle contractility (MGI Ref ID J:55553)
  • reduced cardiac contractility in awake and in anesthetized mice
• decreased heart rate (MGI Ref ID J:55553)
  • decreased heart rate in response to exercise, but normal basal heart rate, normal basal mean arterial blood pressure and normal blood pressure changes in response to exercise
• homeostasis/metabolism phenotype
• decreased oxygen consumption (MGI Ref ID J:55553)
  • reduced O₂ consumption during exercise and reduced CO₂ production during exercise, but normal total exercise capacity
• muscle phenotype
• decreased cardiac muscle contractility (MGI Ref ID J:55553)
  • reduced cardiac contractility in awake and in anesthetized mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Adrb1^tm1Bkk related

Cardiovascular Research
Heart Abnormalities (chronotropy)

Metabolism Research
Exertion Related

Neurobiology Research
Metabolic Defects
Response to Catecholamines

Adrb2^tm1Bkk related

Cardiovascular Research
Heart Abnormalities (chronotropy)

Metabolism Research
Exertion Related

Neurobiology Research
Metabolic Defects
Response to Catecholamines

Genes & Alleles

Gene & Allele Information

Allele Symbol		Adrb1tm1Bkk
Allele Name		targeted mutation 1, Brian K Kobilka
Allele Type		Targeted (knock-out)
Common Name(s)		Adrb1tm1Gsb; Beta1-KO; beta1-AR-;
Mutation Made By		Brian Kobilka,   Stanford University
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Adrb1, adrenergic receptor, beta 1
Chromosome		19
Gene Common Name(s)		ADRB1R; Adrb-1; B1AR; BETA1AR; RATB1AR; RHR; beta 1-AR;
Molecular Note		A 1364 bp genomic fragment containing the initiation codon was replaced with a neomycin selection cassette. No protein product was detected on Western blots of heart tissue from homozygous mutant mice. [MGI Ref ID J:34659]
Allele Symbol		Adrb2tm1Bkk
Allele Name		targeted mutation 1, Brian K Kobilka
Allele Type		Targeted (knock-out)
Common Name(s)		Beta2-AR; Beta2-KO; beta2AR-;
Mutation Made By		Brian Kobilka,   Stanford University
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Adrb2, adrenergic receptor, beta 2
Chromosome		18
Gene Common Name(s)		ADRB2R; ADRBR; Adrb-2; B-adrenergic binding; B2AR; BAR; BETA2AR; Badm; G protein coupled receptor 7; Gpcr7; beta 2-AR; beta 2-adrenoceptor;
Molecular Note		A neomycin selection cassette was inserted into the sequence encoding the fourth transmembrane segment. This mutation is predicted to eliminate expression of a functional receptor. Ligand binding experiments confirmed that no functional receptor was expressed in the lung of homozygous mice. [MGI Ref ID J:55552]

Genotyping

Genotyping Information

Genotyping Protocols

Adrb1^tm1Bkk, SEP PCR, vers. 1
Adrb2^tm1Bkk, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Rohrer DK; Chruscinski A; Schauble EH; Bernstein D; Kobilka BK. 1999. Cardiovascular and metabolic alterations in mice lacking both beta1- and beta2-adrenergic receptors. J Biol Chem 274(24):16701-8. [PubMed: 10358009]  [MGI Ref ID J:55553]

Additional References

Adrb1^tm1Bkk related

Bachman ES; Dhillon H; Zhang CY; Cinti S; Bianco AC; Kobilka BK; Lowell BB. 2002. betaAR signaling required for diet-induced thermogenesis and obesity resistance. Science 297(5582):843-5. [PubMed: 12161655]  [MGI Ref ID J:79309]

Bernstein D; Fajardo G; Zhao M; Urashima T; Powers J; Berry G; Kobilka BK. 2005. Differential cardioprotective/cardiotoxic effects mediated by beta-adrenergic receptor subtypes. Am J Physiol Heart Circ Physiol 289(6):H2441-9. [PubMed: 16040722]  [MGI Ref ID J:104748]

Chruscinski A; Brede ME; Meinel L; Lohse MJ; Kobilka BK; Hein L. 2001. Differential distribution of beta-adrenergic receptor subtypes in blood vessels of knockout mice lacking beta(1)- or beta(2)-adrenergic receptors. Mol Pharmacol 60(5):955-62. [PubMed: 11641423]  [MGI Ref ID J:102882]

Devic E; Xiang Y; Gould D; Kobilka B. 2001. Beta-adrenergic receptor subtype-specific signaling in cardiac myocytes from beta(1) and beta(2) adrenoceptor knockout mice. Mol Pharmacol 60(3):577-83. [PubMed: 11502890]  [MGI Ref ID J:103958]

Ecker PM; Lin CC; Powers J; Kobilka BK; Dubin AM; Bernstein D. 2006. Effect of targeted deletions of beta1- and beta2-adrenergic-receptor subtypes on heart rate variability. Am J Physiol Heart Circ Physiol 290(1):H192-9. [PubMed: 16113068]  [MGI Ref ID J:104760]

Fajardo G; Zhao M; Powers J; Bernstein D. 2006. Differential cardiotoxic/cardioprotective effects of beta-adrenergic receptor subtypes in myocytes and fibroblasts in doxorubicin cardiomyopathy. J Mol Cell Cardiol 40(3):375-383. [PubMed: 16458323]  [MGI Ref ID J:105959]

Jimenez M; Leger B; Canola K; Lehr L; Arboit P; Seydoux J; Russell AP; Giacobino JP; Muzzin P; Preitner F. 2002. beta(1)/beta(2)/beta(3)-adrenoceptor knockout mice are obese and cold-sensitive but have normal lipolytic responses to fasting. FEBS Lett 530(1-3):37. [PubMed: 12387862]  [MGI Ref ID J:79769]

Kim SM; Huang Y; Qin Y; Mizel D; Schnermann J; Briggs JP. 2008. Persistence of circadian variation in arterial blood pressure in {beta}1/{beta}2-adrenergic receptor-deficient mice. Am J Physiol Regul Integr Comp Physiol 294(5):R1427-34. [PubMed: 18305025]  [MGI Ref ID J:134689]

Lehr L; Canola K; Asensio C; Jimenez M; Kuehne F; Giacobino JP; Muzzin P. 2006. The control of UCP1 is dissociated from that of PGC-1alpha or of mitochondriogenesis as revealed by a study using beta-less mouse brown adipocytes in culture. FEBS Lett 580(19):4661-6. [PubMed: 16876797]  [MGI Ref ID J:112155]

Lehr L; Kuehne F; Arboit P; Giacobino JP; Poulin F; Muzzin P; Jimenez M. 2004. Control of 4E-BP1 expression in mouse brown adipose tissue by the beta3-adrenoceptor. FEBS Lett 576(1-2):179-82. [PubMed: 15474034]  [MGI Ref ID J:108474]

McGraw DW; Almoosa KF; Paul RJ; Kobilka BK; Liggett SB. 2003. Antithetic regulation by beta-adrenergic receptors of Gq receptor signaling via phospholipase C underlies the airway beta-agonist paradox. J Clin Invest 112(4):619-26. [PubMed: 12925702]  [MGI Ref ID J:85126]

Miura S; Kawanaka K; Kai Y; Tamura M; Goto M; Shiuchi T; Minokoshi Y; Ezaki O. 2007. An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation. Endocrinology 148(7):3441-8. [PubMed: 17446185]  [MGI Ref ID J:129633]

Nogueiras R; Wiedmer P; Perez-Tilve D; Veyrat-Durebex C; Keogh JM; Sutton GM; Pfluger PT; Castaneda TR; Neschen S; Hofmann SM; Howles PN; Morgan DA; Benoit SC; Szanto I; Schrott B; Schurmann A; Joost HG; Hammond C; Hui DY; Woods SC; Rahmouni K; Butler AA; Farooqi IS; O'Rahilly S; Rohner-Jeanrenaud F; Tschop MH. 2007. The central melanocortin system directly controls peripheral lipid metabolism. J Clin Invest 117(11):3475-88. [PubMed: 17885689]  [MGI Ref ID J:127528]

Rohrer DK; Desai KH; Jasper JR; Stevens ME; Regula DP Jr; Barsh GS; Bernstein D; Kobilka BK. 1996. Targeted disruption of the mouse beta1-adrenergic receptor gene: developmental and cardiovascular effects. Proc Natl Acad Sci U S A 93(14):7375-80. [PubMed: 8693001]  [MGI Ref ID J:34659]

Rohrer DK; Schauble EH; Desai KH; Kobilka BK; Bernstein D. 1998. Alterations in dynamic heart rate control in the beta 1-adrenergic receptor knockout mouse. Am J Physiol 274(4 Pt 2):H1184-93. [PubMed: 9575921]  [MGI Ref ID J:47167]

Swoap SJ; Li C; Wess J; Parsons AD; Williams TD; Overton JM. 2008. Vagal tone dominates autonomic control of mouse heart rate at thermoneutrality. Am J Physiol Heart Circ Physiol 294(4):H1581-8. [PubMed: 18245567]  [MGI Ref ID J:135270]

Tavernier G; Jimenez M; Giacobino JP; Hulo N; Lafontan M; Muzzin P; Langin D. 2005. Norepinephrine induces lipolysis in beta1/beta2/beta3-adrenoceptor knockout mice. Mol Pharmacol 68(3):793-9. [PubMed: 15939797]  [MGI Ref ID J:114333]

Theander-Carrillo C; Wiedmer P; Cettour-Rose P; Nogueiras R; Perez-Tilve D; Pfluger P; Castaneda TR; Muzzin P; Schurmann A; Szanto I; Tschop MH; Rohner-Jeanrenaud F. 2006. Ghrelin action in the brain controls adipocyte metabolism. J Clin Invest 116(7):1983-93. [PubMed: 16767221]  [MGI Ref ID J:111740]

Wang Y; De Arcangelis V; Gao X; Ramani B; Jung YS; Xiang Y. 2008. Norepinephrine- and epinephrine-induced distinct beta2-adrenoceptor signaling is dictated by GRK2 phosphorylation in cardiomyocytes. J Biol Chem 283(4):1799-807. [PubMed: 18056263]  [MGI Ref ID J:130709]

Adrb2^tm1Bkk related

Bachman ES; Dhillon H; Zhang CY; Cinti S; Bianco AC; Kobilka BK; Lowell BB. 2002. betaAR signaling required for diet-induced thermogenesis and obesity resistance. Science 297(5582):843-5. [PubMed: 12161655]  [MGI Ref ID J:79309]

Bernstein D; Fajardo G; Zhao M; Urashima T; Powers J; Berry G; Kobilka BK. 2005. Differential cardioprotective/cardiotoxic effects mediated by beta-adrenergic receptor subtypes. Am J Physiol Heart Circ Physiol 289(6):H2441-9. [PubMed: 16040722]  [MGI Ref ID J:104748]

Chruscinski A; Brede ME; Meinel L; Lohse MJ; Kobilka BK; Hein L. 2001. Differential distribution of beta-adrenergic receptor subtypes in blood vessels of knockout mice lacking beta(1)- or beta(2)-adrenergic receptors. Mol Pharmacol 60(5):955-62. [PubMed: 11641423]  [MGI Ref ID J:102882]

Chruscinski AJ; Rohrer DK; Schauble E; Desai KH; Bernstein D; Kobilka BK. 1999. Targeted disruption of the beta2 adrenergic receptor gene. J Biol Chem 274(24):16694-700. [PubMed: 10358008]  [MGI Ref ID J:55552]

Devic E; Xiang Y; Gould D; Kobilka B. 2001. Beta-adrenergic receptor subtype-specific signaling in cardiac myocytes from beta(1) and beta(2) adrenoceptor knockout mice. Mol Pharmacol 60(3):577-83. [PubMed: 11502890]  [MGI Ref ID J:103958]

Ecker PM; Lin CC; Powers J; Kobilka BK; Dubin AM; Bernstein D. 2006. Effect of targeted deletions of beta1- and beta2-adrenergic-receptor subtypes on heart rate variability. Am J Physiol Heart Circ Physiol 290(1):H192-9. [PubMed: 16113068]  [MGI Ref ID J:104760]

Elefteriou F; Ahn JD; Takeda S; Starbuck M; Yang X; Liu X; Kondo H; Richards WG; Bannon TW; Noda M; Clement K; Vaisse C; Karsenty G. 2005. Leptin regulation of bone resorption by the sympathetic nervous system and CART. Nature 434(7032):514-20. [PubMed: 15724149]  [MGI Ref ID J:97566]

Fajardo G; Zhao M; Powers J; Bernstein D. 2006. Differential cardiotoxic/cardioprotective effects of beta-adrenergic receptor subtypes in myocytes and fibroblasts in doxorubicin cardiomyopathy. J Mol Cell Cardiol 40(3):375-383. [PubMed: 16458323]  [MGI Ref ID J:105959]

Ghoghawala SY; Mannis MJ; Pullar CE; Rosenblatt MI; Isseroff RR. 2008. Beta2-adrenergic receptor signaling mediates corneal epithelial wound repair. Invest Ophthalmol Vis Sci 49(5):1857-63. [PubMed: 18436820]  [MGI Ref ID J:135178]

Jimenez M; Leger B; Canola K; Lehr L; Arboit P; Seydoux J; Russell AP; Giacobino JP; Muzzin P; Preitner F. 2002. beta(1)/beta(2)/beta(3)-adrenoceptor knockout mice are obese and cold-sensitive but have normal lipolytic responses to fasting. FEBS Lett 530(1-3):37. [PubMed: 12387862]  [MGI Ref ID J:79769]

Kasprowicz DJ; Kohm AP; Berton MT; Chruscinski AJ; Sharpe A; Sanders VM. 2000. Stimulation of the B cell receptor, CD86 (B7-2), and the beta 2-adrenergic receptor intrinsically modulates the level of IgG1 and IgE produced per B cell. J Immunol 165(2):680-90. [PubMed: 10878340]  [MGI Ref ID J:120527]

Kim SM; Huang Y; Qin Y; Mizel D; Schnermann J; Briggs JP. 2008. Persistence of circadian variation in arterial blood pressure in {beta}1/{beta}2-adrenergic receptor-deficient mice. Am J Physiol Regul Integr Comp Physiol 294(5):R1427-34. [PubMed: 18305025]  [MGI Ref ID J:134689]

Lehr L; Canola K; Asensio C; Jimenez M; Kuehne F; Giacobino JP; Muzzin P. 2006. The control of UCP1 is dissociated from that of PGC-1alpha or of mitochondriogenesis as revealed by a study using beta-less mouse brown adipocytes in culture. FEBS Lett 580(19):4661-6. [PubMed: 16876797]  [MGI Ref ID J:112155]

Lehr L; Kuehne F; Arboit P; Giacobino JP; Poulin F; Muzzin P; Jimenez M. 2004. Control of 4E-BP1 expression in mouse brown adipose tissue by the beta3-adrenoceptor. FEBS Lett 576(1-2):179-82. [PubMed: 15474034]  [MGI Ref ID J:108474]

Liang DY; Liao G; Wang J; Usuka J; Guo Y; Peltz G; Clark JD. 2006. A genetic analysis of opioid-induced hyperalgesia in mice. Anesthesiology 104(5):1054-62. [PubMed: 16645459]  [MGI Ref ID J:108835]

Liang DY; Shi X; Li X; Li J; Clark JD. 2007. The beta2 adrenergic receptor regulates morphine tolerance and physical dependence. Behav Brain Res 181(1):118-26. [PubMed: 17498818]  [MGI Ref ID J:121984]

McGraw DW; Almoosa KF; Paul RJ; Kobilka BK; Liggett SB. 2003. Antithetic regulation by beta-adrenergic receptors of Gq receptor signaling via phospholipase C underlies the airway beta-agonist paradox. J Clin Invest 112(4):619-26. [PubMed: 12925702]  [MGI Ref ID J:85126]

Mendez-Ferrer S; Lucas D; Battista M; Frenette PS. 2008. Haematopoietic stem cell release is regulated by circadian oscillations. Nature 452(7186):442-7. [PubMed: 18256599]  [MGI Ref ID J:134224]

Miura S; Kawanaka K; Kai Y; Tamura M; Goto M; Shiuchi T; Minokoshi Y; Ezaki O. 2007. An increase in murine skeletal muscle peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) mRNA in response to exercise is mediated by beta-adrenergic receptor activation. Endocrinology 148(7):3441-8. [PubMed: 17446185]  [MGI Ref ID J:129633]

Mutlu GM; Dumasius V; Burhop J; McShane PJ; Meng FJ; Welch L; Dumasius A; Mohebahmadi N; Thakuria G; Hardiman K; Matalon S; Hollenberg S; Factor P. 2004. Upregulation of alveolar epithelial active Na+ transport is dependent on beta2-adrenergic receptor signaling. Circ Res 94(8):1091-100. [PubMed: 15016730]  [MGI Ref ID J:98914]

Nogueiras R; Wiedmer P; Perez-Tilve D; Veyrat-Durebex C; Keogh JM; Sutton GM; Pfluger PT; Castaneda TR; Neschen S; Hofmann SM; Howles PN; Morgan DA; Benoit SC; Szanto I; Schrott B; Schurmann A; Joost HG; Hammond C; Hui DY; Woods SC; Rahmouni K; Butler AA; Farooqi IS; O'Rahilly S; Rohner-Jeanrenaud F; Tschop MH. 2007. The central melanocortin system directly controls peripheral lipid metabolism. J Clin Invest 117(11):3475-88. [PubMed: 17885689]  [MGI Ref ID J:127528]

Pongratz G; McAlees JW; Conrad DH; Erbe RS; Haas KM; Sanders VM. 2006. The level of IgE produced by a B cell is regulated by norepinephrine in a p38 MAPK- and CD23-dependent manner. J Immunol 177(5):2926-38. [PubMed: 16920928]  [MGI Ref ID J:139551]

Sanders VM; Kasprowicz DJ; Swanson-Mungerson MA; Podojil JR; Kohm AP. 2003. Adaptive immunity in mice lacking the beta(2)-adrenergic receptor. Brain Behav Immun 17(1):55-67. [PubMed: 12615050]  [MGI Ref ID J:82380]

Sato S; Hanada R; Kimura A; Abe T; Matsumoto T; Iwasaki M; Inose H; Ida T; Mieda M; Takeuchi Y; Fukumoto S; Fujita T; Kato S; Kangawa K; Kojima M; Shinomiya K; Takeda S. 2007. Central control of bone remodeling by neuromedin U. Nat Med 13(10):1234-40. [PubMed: 17873881]  [MGI Ref ID J:129930]

Swoap SJ; Li C; Wess J; Parsons AD; Williams TD; Overton JM. 2008. Vagal tone dominates autonomic control of mouse heart rate at thermoneutrality. Am J Physiol Heart Circ Physiol 294(4):H1581-8. [PubMed: 18245567]  [MGI Ref ID J:135270]

Tavernier G; Jimenez M; Giacobino JP; Hulo N; Lafontan M; Muzzin P; Langin D. 2005. Norepinephrine induces lipolysis in beta1/beta2/beta3-adrenoceptor knockout mice. Mol Pharmacol 68(3):793-9. [PubMed: 15939797]  [MGI Ref ID J:114333]

Theander-Carrillo C; Wiedmer P; Cettour-Rose P; Nogueiras R; Perez-Tilve D; Pfluger P; Castaneda TR; Muzzin P; Schurmann A; Szanto I; Tschop MH; Rohner-Jeanrenaud F. 2006. Ghrelin action in the brain controls adipocyte metabolism. J Clin Invest 116(7):1983-93. [PubMed: 16767221]  [MGI Ref ID J:111740]

Wang Y; De Arcangelis V; Gao X; Ramani B; Jung YS; Xiang Y. 2008. Norepinephrine- and epinephrine-induced distinct beta2-adrenoceptor signaling is dictated by GRK2 phosphorylation in cardiomyocytes. J Biol Chem 283(4):1799-807. [PubMed: 18056263]  [MGI Ref ID J:130709]

de Coupade C; Brown AS; Dazin PF; Levine JD; Green PG. 2007. beta(2)-Adrenergic receptor-dependent sexual dimorphism for murine leukocyte migration. J Neuroimmunol 186(1-2):54-62. [PubMed: 17442405]  [MGI Ref ID J:124560]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & Husbandry	These mice are maintained by mating double homozygote null mice. A number of strains have contributed to the background (C57BL/6J, DBA/2, 129, FVB/N, CD-1) which will be designated as STOCK.
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	None Available
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.