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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK syfp-3J (Changed: 15-DEC-04 ) Type Mutant Stock; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1 (25-JUL-04)
Generation DefinitionsDescription
Mice homozygous for the recessive fused phalanges 3 Jackson mutation (Fbn2fp-3J) have syndactyly of the second, third, and/or the fourth digits of the hindlimbs and sometimes also the frontlimbs (Munroe et al., 2000; Browning et al., 2001.) This syndactyly likely results from defective mesenchyme differentiation rather than failed interdigital apoptosis (Arteaga-Solis et al., 2001).Development
The v6.4 ES cell line was derived from (129S4/SvJae x C57BL/6J) F1 embryo. V6.4 ES cells were treated with ethylmethanesulphonate and injected into blastocysts. The resulting chimeras were bred with C57BL/6J mates and their offspring were backcrossed again to C57BL/6J. These G2 offspring were then backcrossed to their G1 parent to produce G3 offspring in which the recessive Fbn2fp-3J mutation was then first recognized. The chimera that transmitted this mutation was v6.4-C7. For mapping purposes, homozygous males were subsequently bred to CAST/Ei females. Sperm were cryopreserved from homozygous male progeny on this mixed background.
Related Strains
Strains carrying other alleles of Fbn2
002995 B6 x C.B10-H2b/LiMcdJ-Fbn2fp-2J/J 000244 C3Fe(B6)-Fbn2fp/J View Strains carrying other alleles of Fbn2 (2 strains)
Phenotype
Phenotype Information
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Arthrogryposis, Distal, Type 9; DA9 (FBN2)
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Fbn2fp-3J/Fbn2fp-3J
involves: 129S4/SvJae * C57BL/6J
- limbs/digits/tail phenotype
- syndactyly
- variable penetrance, usually involving digits 2, 3, and/or 4, usually on hindlimb; rarely also on forelimb (MGI Ref ID J:60747)
This mouse can be used to support research in many areas including:Fbn2fp-3J related
Developmental Biology Research
Skeletal Defects
syndactyly
Mouse/Human Gene Homologs
congenital contractural arachnodactyly
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Fbn2fp-3J | ||
|---|---|---|---|
| Allele Name | fused phalanges 3 Jackson | ||
| Allele Type | Chemically induced (other) | ||
| Common Name(s) | Fbn2fp-3J; Fbn2fp-sne; sne; | ||
| Strain of Origin | (C57BL/6J x 129S4/SvJae)F1 | ||
| ES Cell Line Name | v6.4 | ||
| ES Cell Line Strain | (C57BL/6J x 129S4/SvJae)F1 | ||
| Gene Symbol and Name | Fbn2, fibrillin 2 | ||
| Chromosome | 18 | ||
| Gene Common Name(s) | BC063774; CCA; DA9; Fib-2; Sne; cDNA sequence BC063774; sne; sy; syndatyly ems; | ||
| General Note | v6.4 ES cells were heavily mutagenized with ethylmethanesulphonate (EMS). | ||
| Molecular Note | The 3' end of a LINE element, including an approximately 120-bp poly(A) tail, 657 bp of 3'UTR, and 61 amino acids from the 3' end of ORF2, replaced a 672 bp segment of the gene including 465 bp of the 3' end of intron 57, exon 58 in its entirety, and 81 bp of the 5' end of intron 59. [MGI Ref ID J:69450] | ||
References
References provided by MGI
Additional References
Arteaga-Solis E; Gayraud B; Lee SY; Shum L; Sakai L; Ramirez F. 2001. Regulation of limb patterning by extracellular microfibrils. J Cell Biol 154(2):275-81. [PubMed: 11470817] [MGI Ref ID J:70592]
Browning VL; Chaudhry SS; Planchart A; Dixon MJ; Schimenti JC. 2001. Mutations of the Mouse Twist and sy (Fibrillin 2) Genes Induced by Chemical Mutagenesis of ES Cells. Genomics 73(3):291-8. [PubMed: 11350121] [MGI Ref ID J:69450]
Munroe RJ; Bergstrom RA; Zheng QY; Libby B; Smith R; John SW; Schimenti KJ; Browning VL; Schimenti JC. 2000. Mouse mutants from chemically mutagenized embryonic stem cells. Nat Genet 24(3):318-21. [PubMed: 10700192] [MGI Ref ID J:60747]
Fbn2fp-3J relatedBrowning VL; Chaudhry SS; Planchart A; Dixon MJ; Schimenti JC. 2001. Mutations of the Mouse Twist and sy (Fibrillin 2) Genes Induced by Chemical Mutagenesis of ES Cells. Genomics 73(3):291-8. [PubMed: 11350121] [MGI Ref ID J:69450]
Munroe RJ; Bergstrom RA; Zheng QY; Libby B; Smith R; John SW; Schimenti KJ; Browning VL; Schimenti JC. 2000. Mouse mutants from chemically mutagenized embryonic stem cells. Nat Genet 24(3):318-21. [PubMed: 10700192] [MGI Ref ID J:60747]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3000.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery of Strains Needing Progeny Testing.
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks.Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery of Strains Needing Progeny Testing.
At least two untested males and two untested females (two pairs) will be recovered (eight or more mice is typical). The total number of animals provided, their gender and genotype will vary. Untested animals typically are available to ship between 13 and 16 weeks from the date of your order. If the first recovery attempt is unsuccessful, a second recovery will be done, extending the overall recovery time to approximately 25 weeks.Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 (from U.S.A., Canada and Puerto Rico only) or 1-207-288-5845 (from any location). The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Payment Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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