Strain Name:

B6.129S6-Naglutm1Efn/J

Stock Number:

003827

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Elizabeth F. Neufeld,   UCLA School of Medicine

Description
At birth, homozygous null mice are viable, normal in size, and do not display any gross physical or behavioral abnormalities. The lysosomal enzyme alpha-N-acetylglucosaminidase (Naglu) is absent in all tissues. Large amounts of heparan sulfate accumulate in liver and kidney, and lesser amounts in other organs. At one month of age, vacuolated macrophages can be found in most tissues. Epithelial cells in kidney and neurons in some parts of the brain are also affected. The vacuolation becomes more prominent with age. At 4-5 months, the mice show abnormal behavior in an open field test. The life span is 8-12 months. Older animals may have urinary retention and difficulty walking and must be euthanized. The null mice were originally described as fertile, but their fertility has decreasd markedly with repeated back-crossing to an inbred strain. These mice are suitable for examining the pathophysiology of the Sanfilippo syndrome type B and for developing therapies for this lysosomal storage disorder.

Development
A targeting vector containing a neomycin resistance gene driven by the mouse phosphoglycerate kinase promoter and a Herpes simplex virus thymidine kinase gene driven by its own promoter was used to disrupt a portion of exon 6. The construct was electroporated into 129S6/SvEv-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric male animals were backcrossed to C57BL/6 females.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Mucopolysaccharidosis, Type IIIB; MPS3B
Models with phenotypic similarity to human diseases where etiology is unknown or involving genes where ortholog is unknown.
Otitis Media, Susceptibility to
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Naglutm1Efn/Naglutm1Efn

        either: (involves: 129S/SvEv * C57BL/6) or (involves: C57BL/6)
  • mortality/aging
  • premature death
    • homozygotes survive to 8-12 months   (MGI Ref ID J:58950)
    • appear normal, healthy, and fertile up to 6 months of age   (MGI Ref ID J:58950)
  • behavior/neurological phenotype
  • abnormal locomotor behavior
    • difficulty walking after about 6 months of age   (MGI Ref ID J:58950)
  • decreased fear-related response
    • older mice demonstrate a reduced freezing response to the tone used in fear tests   (MGI Ref ID J:58950)
  • increased anxiety-related response
    • suppressed open field activity in mice over 4.5 months of age   (MGI Ref ID J:58950)
  • growth/size/body phenotype
  • distended abdomen
    • after 6 months of age   (MGI Ref ID J:58950)
  • weight loss
    • weight loss starts to occur at some time after 6 months of age   (MGI Ref ID J:58950)
  • hematopoietic system phenotype
  • abnormal macrophage morphology
    • by 33 days of age, vacuolated macrophages are found in many organs   (MGI Ref ID J:58950)
    • vacuolated macrophages become more widely distributed with age to 6 months   (MGI Ref ID J:58950)
    • abnormal Kupffer cell morphology
      • Kupffer cells are ballooned and contain very few large inclusions   (MGI Ref ID J:58950)
  • immune system phenotype
  • abnormal macrophage morphology
    • by 33 days of age, vacuolated macrophages are found in many organs   (MGI Ref ID J:58950)
    • vacuolated macrophages become more widely distributed with age to 6 months   (MGI Ref ID J:58950)
    • abnormal Kupffer cell morphology
      • Kupffer cells are ballooned and contain very few large inclusions   (MGI Ref ID J:58950)
  • liver/biliary system phenotype
  • abnormal Kupffer cell morphology
    • Kupffer cells are ballooned and contain very few large inclusions   (MGI Ref ID J:58950)
  • abnormal hepatocyte morphology
    • hepatocytes contain many large vacuoles   (MGI Ref ID J:58950)
  • renal/urinary system phenotype
  • abnormal podocyte morphology
    • vacuolated glomerular epithelial cells seen by 33 days of age   (MGI Ref ID J:58950)
    • extent of vacuolation increases with age to at least 6 months   (MGI Ref ID J:58950)
  • abnormal renal tubule epithelium morphology
    • by 33 days of age, epithelial cells are vacuolated in distal but not proximal convoluted tubules, and in Henle's loop   (MGI Ref ID J:58950)
    • at 3 to 6 months of age, more prominent vacuolation is seen in tubular epithelial cells as well as interstitial cells   (MGI Ref ID J:58950)
    • however, mesangial cells are not vacuolated   (MGI Ref ID J:58950)
  • ischuria
    • urinary retention in the bladder starting at 6 months of age   (MGI Ref ID J:58950)
  • nervous system phenotype
  • abnormal accessory olfactory bulb morphology
    • accessory olfactory bulb with vacuolated neurons   (MGI Ref ID J:58950)
  • abnormal amygdala morphology
    • some vacuolated neurons   (MGI Ref ID J:58950)
  • abnormal cerebellum deep nucleus morphology
    • neurons of cerebellar nuclei containing one or more inclusions similar to those found in Purkinje cells   (MGI Ref ID J:58950)
  • abnormal cerebral cortex morphology
    • some areas of the cortex contain vacuolated neurons   (MGI Ref ID J:58950)
  • abnormal diencephalon morphology
    • neurons in the thalamus and the hypothalamus with vacuoles   (MGI Ref ID J:58950)
  • abnormal midbrain morphology   (MGI Ref ID J:58950)
  • abnormal neuron morphology
    • by 33 days of age, some neurons in various regions of the brain become vacuolated   (MGI Ref ID J:58950)
    • extent of vacuolation increases with age   (MGI Ref ID J:58950)
    • abnormal Purkinje cell morphology
      • rather than vacuoles cells may contain one or more large periodic acid/Schiff positive inclusions   (MGI Ref ID J:58950)
  • abnormal olfactory bulb morphology
    • olfactory bulb with vacuolated neurons   (MGI Ref ID J:58950)
  • abnormal pons morphology
    • some vacuolated neurons   (MGI Ref ID J:58950)
  • cardiovascular system phenotype
  • abnormal Kupffer cell morphology
    • Kupffer cells are ballooned and contain very few large inclusions   (MGI Ref ID J:58950)
  • integument phenotype
  • disheveled coat
    • after 6 months of age   (MGI Ref ID J:58950)
  • spontaneous skin ulceration
    • skin ulceration around the genitalia occurs after 6 months of age   (MGI Ref ID J:58950)

Naglutm1Efn/Naglutm1Efn

        involves: 129S/SvEv * C57BL/6J
  • mortality/aging
  • premature death
    • average age of death is 315-360 days   (MGI Ref ID J:129390)
  • cellular phenotype
  • abnormal lysosome morphology
    • mutants exhibit lysosomal distention in multiple tissues, including in the middle and inner ear, in the eye, and in the suprachiasmatic nucleus   (MGI Ref ID J:129390)
  • behavior/neurological phenotype
  • abnormal circadian rhythm
    • percentage of daily activity which occurs during the light portion of the LD cycle is increased in mutants   (MGI Ref ID J:129390)
    • delayed circadian phase
      • mutants differ from wild-type in their phase angle of entrainment (the time from the light offset to the onset of daily locomotor activity); mutants start their daily activity about 1 hour later than wild-type   (MGI Ref ID J:129390)
  • impaired coordination
    • rotarod testing shows a progressive inability of older, but not younger, mutants to coordinate movement in a rocking paradigm   (MGI Ref ID J:129390)
  • nervous system phenotype
  • Purkinje cell degeneration
    • age dependent loss of Purkinje cells   (MGI Ref ID J:129390)
  • abnormal suprachiasmatic nucleus morphology
    • the suprachiasmatic nucleus (SCN) shows a large number of pyknotic cells with condensed nuclei and cytoplasms and distended lysosomes   (MGI Ref ID J:129390)
  • decreased Purkinje cell number
    • diffuse decrease in cerebellar Purkinje neuronal counts at the vermis in older, but not younger, mutants   (MGI Ref ID J:129390)
  • decreased cochlear hair cell number
    • complete loss of hair cells in the lower base coincides with degeneration of the organ of Corti   (MGI Ref ID J:129390)
  • short photoreceptor outer segment
    • progressive shortening of the outer segments   (MGI Ref ID J:129390)
  • hearing/vestibular/ear phenotype
  • abnormal inner ear morphology   (MGI Ref ID J:129390)
    • abnormal cochlea morphology
      • inflammatory cells are more likely to appear in the perilymphatic scalae of mutants than wild-type and often show lysosomal storage   (MGI Ref ID J:129390)
      • abnormal Reissner membrane morphology
        • lysosomal storage is seen in Reissner's membrane   (MGI Ref ID J:129390)
      • abnormal organ of Corti morphology
        • lysosomal storage in the organ of Corti occurs within outer sulcus cells of the lateral organ and pillar cells of the medial organ   (MGI Ref ID J:129390)
        • decreased cochlear hair cell number
          • complete loss of hair cells in the lower base coincides with degeneration of the organ of Corti   (MGI Ref ID J:129390)
        • organ of Corti degeneration   (MGI Ref ID J:129390)
      • abnormal spiral ligament morphology
        • lysosomal storage is seen in the spiral ligament   (MGI Ref ID J:129390)
      • abnormal spiral limbus morphology
        • lysosomal storage is seen in the spiral limbus   (MGI Ref ID J:129390)
    • abnormal crista ampullaris morphology
      • abnormal lysosomal storage in the crista ampullaris of the lateral semicircular canal   (MGI Ref ID J:129390)
    • abnormal inner ear vestibule morphology
      • the vestibular maculae and cristae show prominent lysosome storage in both supporting cells and hair cells and the dark cells of the cristae   (MGI Ref ID J:129390)
  • abnormal middle ear morphology
    • middle ears at 30 weeks of age show lysosomal storage-related anomalies   (MGI Ref ID J:129390)
    • lysosomal storage is prominent in the mucosal lining and within osteocytes, chondrocytes, and inflammatory cells   (MGI Ref ID J:129390)
    • abnormal middle ear ossicle morphology
      • thickened mucosal layer on stapes and malleus at 30 weeks of age with hyperplasia of mucosal cells   (MGI Ref ID J:129390)
      • abnormal stapes morphology
        • bone surface of the stapes is pitted, indicating abnormal bone remodeling   (MGI Ref ID J:129390)
  • conductive hearing loss
    • auditory brainstem response (ABR) shows progressive hearing deficits   (MGI Ref ID J:129390)
  • increased or absent threshold for auditory brainstem response
    • increase in high frequency ABR thresholds is significantly greater in mutants up to 16 weeks than in controls   (MGI Ref ID J:129390)
  • increased susceptibility to otitis media
    • middle ears at 30 weeks of age exhibit highly variable otitis media, with and without effusion   (MGI Ref ID J:129390)
  • vision/eye phenotype
  • abnormal eye electrophysiology
    • ERG shows a progressive decrease in the amplitude of the dark-adapted b-wave response   (MGI Ref ID J:129390)
    • abnormal rod electrophysiology
      • dark-adapted retinal response is depressed by 5 weeks and becomes progressively less sensitive with increasing age, indicating loss of rod function   (MGI Ref ID J:129390)
      • however, cone function appears normal   (MGI Ref ID J:129390)
  • abnormal retina morphology
    • aberrant lysosomal storage is seen in the inner retina   (MGI Ref ID J:129390)
    • abnormal retinal pigment epithelium morphology
      • localized disruption and inclusions in the retinal pigment epithelium   (MGI Ref ID J:129390)
    • short photoreceptor outer segment
      • progressive shortening of the outer segments   (MGI Ref ID J:129390)
    • thin retinal outer nuclear layer
      • progressive reduction of the outer nuclear layer   (MGI Ref ID J:129390)
  • abnormal sclera morphology
    • lysosomal storage in the sclera   (MGI Ref ID J:129390)
  • immune system phenotype
  • increased susceptibility to otitis media
    • middle ears at 30 weeks of age exhibit highly variable otitis media, with and without effusion   (MGI Ref ID J:129390)
  • craniofacial phenotype
  • abnormal middle ear ossicle morphology
    • thickened mucosal layer on stapes and malleus at 30 weeks of age with hyperplasia of mucosal cells   (MGI Ref ID J:129390)
    • abnormal stapes morphology
      • bone surface of the stapes is pitted, indicating abnormal bone remodeling   (MGI Ref ID J:129390)
  • pigmentation phenotype
  • abnormal retinal pigment epithelium morphology
    • localized disruption and inclusions in the retinal pigment epithelium   (MGI Ref ID J:129390)
  • skeleton phenotype
  • abnormal middle ear ossicle morphology
    • thickened mucosal layer on stapes and malleus at 30 weeks of age with hyperplasia of mucosal cells   (MGI Ref ID J:129390)
    • abnormal stapes morphology
      • bone surface of the stapes is pitted, indicating abnormal bone remodeling   (MGI Ref ID J:129390)
  • abnormal spiral ligament morphology
    • lysosomal storage is seen in the spiral ligament   (MGI Ref ID J:129390)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Naglutm1Efn/Naglutm1Efn

        involves: 129S/SvEv * C57BL/6
  • nervous system phenotype
  • abnormal cerebral cortex morphology
    • vacuolated microglial cells sometimes apposed to neurons   (MGI Ref ID J:81974)

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Naglutm1Efn
Allele Name targeted mutation 1, Elizabeth F Neufeld
Allele Type Targeted (Null/Knockout)
Common Name(s) MPS IIIB; Naglu-;
Mutation Made By Elizabeth Neufeld,   UCLA School of Medicine
Strain of Origin129S/SvEv-Gpi1
ES Cell Line NameCCE/EK.CCE
ES Cell Line Strain129S/SvEv-Gpi1
Gene Symbol and Name Naglu, alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB)
Chromosome 11
Gene Common Name(s) MPS-IIIB; MPS3B; NAG; RGD1564228; UFHSD;
Molecular Note Exon 6 of this gene, a region known to harbor several Sanfilippo B mutations, was disrupted by the insertion of a neomycin resistance gene via homologous recombination. Protein from homozygous mutant animals was negative for enzyme activity. [MGI Ref ID J:58950]

Genotyping

Genotyping Information

Genotyping Protocols

Naglutm1Efn, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Gografe SI; Garbuzova-Davis S; Willing AE; Haas K; Chamizo W; Sanberg PR. 2003. Mouse model of Sanfilippo syndrome type B: relation of phenotypic features to background strain. Comp Med 53(6):622-32. [PubMed: 14727810]  [MGI Ref ID J:87310]

Naglutm1Efn related

Ausseil J; Desmaris N; Bigou S; Attali R; Corbineau S; Vitry S; Parent M; Cheillan D; Fuller M; Maire I; Vanier MT; Heard JM. 2008. Early neurodegeneration progresses independently of microglial activation by heparan sulfate in the brain of mucopolysaccharidosis IIIB mice. PLoS ONE 3(5):e2296. [PubMed: 18509511]  [MGI Ref ID J:136381]

Cheillan D; Malleval C; Ausseil J; Vitry S; Heard JM; Maire I; Honnorat J; Belin MF; Touret M. 2008. Abnormal expression of truncated CRMP-1 protein in the brain cortex of MPSIIIB mice. Mol Genet Metab 94(1):135-8. [PubMed: 18325808]  [MGI Ref ID J:134471]

Cho SK; Gao N; Pearce DA; Lehrman MA; Hofmann SL. 2005. Characterization of lipid-linked oligosaccharide accumulation in mouse models of Batten disease. Glycobiology 15(6):637-48. [PubMed: 15647513]  [MGI Ref ID J:112499]

Cressant A; Desmaris N; Verot L; Brejot T; Froissart R; Vanier MT; Maire I; Heard JM. 2004. Improved behavior and neuropathology in the mouse model of Sanfilippo type IIIB disease after adeno-associated virus-mediated gene transfer in the striatum. J Neurosci 24(45):10229-39. [PubMed: 15537895]  [MGI Ref ID J:96575]

Di Natale P; Di Domenico C; Gargiulo N; Castaldo S; Gonzalez Y Reyero E; Mithbaokar P; De Felice M; Follenzi A; Naldini L; Villani GR. 2005. Treatment of the mouse model of mucopolysaccharidosis type IIIB with lentiviral-NAGLU vector. Biochem J 388(Pt 2):639-46. [PubMed: 15649123]  [MGI Ref ID J:117532]

Fu H; Bartz JD; Stephens RL Jr; McCarty DM. 2012. Peripheral nervous system neuropathology and progressive sensory impairments in a mouse model of Mucopolysaccharidosis IIIB. PLoS One 7(9):e45992. [PubMed: 23049915]  [MGI Ref ID J:191944]

Garbuzova-Davis S; Louis MK; Haller EM; Derasari HM; Rawls AE; Sanberg PR. 2011. Blood-brain barrier impairment in an animal model of MPS III B. PLoS One 6(3):e16601. [PubMed: 21408219]  [MGI Ref ID J:171706]

Gografe SI; Garbuzova-Davis S; Willing AE; Haas K; Chamizo W; Sanberg PR. 2003. Mouse model of Sanfilippo syndrome type B: relation of phenotypic features to background strain. Comp Med 53(6):622-32. [PubMed: 14727810]  [MGI Ref ID J:87310]

Heldermon CD; Hennig AK; Ohlemiller KK; Ogilvie JM; Herzog ED; Breidenbach A; Vogler C; Wozniak DF; Sands MS. 2007. Development of sensory, motor and behavioral deficits in the murine model of Sanfilippo syndrome type B. PLoS ONE 2(1):e772. [PubMed: 17712420]  [MGI Ref ID J:129390]

Langford-Smith A; Malinowska M; Langford-Smith KJ; Wegrzyn G; Jones S; Wynn R; Wraith JE; Wilkinson FL; Bigger BW. 2011. Hyperactive behaviour in the mouse model of mucopolysaccharidosis IIIB in the open field and home cage environments. Genes Brain Behav 10(6):673-82. [PubMed: 21635693]  [MGI Ref ID J:186608]

Langford-Smith K; Arasaradnam M; Wraith JE; Wynn R; Bigger BW. 2010. Evaluation of heparin cofactor II-thrombin complex as a biomarker on blood spots from mucopolysaccharidosis I, IIIA and IIIB mice. Mol Genet Metab 99(3):269-74. [PubMed: 19926322]  [MGI Ref ID J:158031]

Li HH; Yu WH; Rozengurt N; Zhao HZ; Lyons KM; Anagnostaras S; Fanselow MS; Suzuki K; Vanier MT; Neufeld EF. 1999. Mouse model of Sanfilippo syndrome type B produced by targeted disruption of the gene encoding alpha-N-acetylglucosaminidase. Proc Natl Acad Sci U S A 96(25):14505-10. [PubMed: 10588735]  [MGI Ref ID J:58950]

Li HH; Zhao HZ; Neufeld EF; Cai Y; Gomez-Pinilla F. 2002. Attenuated plasticity in neurons and astrocytes in the mouse model of Sanfilippo syndrome type B. J Neurosci Res 69(1):30-8. [PubMed: 12111813]  [MGI Ref ID J:113178]

Malinowska M; Wilkinson FL; Bennett W; Langford-Smith KJ; O'Leary HA; Jakobkiewicz-Banecka J; Wynn R; Wraith JE; Wegrzyn G; Bigger BW. 2009. Genistein reduces lysosomal storage in peripheral tissues of mucopolysaccharide IIIB mice. Mol Genet Metab 98(3):235-42. [PubMed: 19632871]  [MGI Ref ID J:155027]

Mohammed EE; Snella EM; Rutz-Mendicino MM; Echevarria FD; Awedikian R; Whitley EM; Ellinwood NM. 2012. Accelerated clinical disease and pathology in mucopolysaccharidosis type IIIB and GalNAc transferase double knockout mice. Mol Genet Metab 107(1-2):129-35. [PubMed: 22867887]  [MGI Ref ID J:188147]

Ohmi K; Greenberg DS; Rajavel KS; Ryazantsev S; Li HH; Neufeld EF. 2003. Activated microglia in cortex of mouse models of mucopolysaccharidoses I and IIIB. Proc Natl Acad Sci U S A 100(4):1902-7. [PubMed: 12576554]  [MGI Ref ID J:81974]

Ohmi K; Kudo LC; Ryazantsev S; Zhao HZ; Karsten SL; Neufeld EF. 2009. Sanfilippo syndrome type B, a lysosomal storage disease, is also a tauopathy. Proc Natl Acad Sci U S A 106(20):8332-7. [PubMed: 19416848]  [MGI Ref ID J:148534]

Ohmi K; Zhao HZ; Neufeld EF. 2011. Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B. PLoS One 6(11):e27461. [PubMed: 22096577]  [MGI Ref ID J:180969]

Ryazantsev S; Yu WH; Zhao HZ; Neufeld EF; Ohmi K. 2007. Lysosomal accumulation of SCMAS (subunit c of mitochondrial ATP synthase) in neurons of the mouse model of mucopolysaccharidosis III B. Mol Genet Metab 90(4):393-401. [PubMed: 17185018]  [MGI Ref ID J:121457]

Villani GR; Di Domenico C; Musella A; Cecere F; Di Napoli D; Di Natale P. 2009. Mucopolysaccharidosis IIIB: oxidative damage and cytotoxic cell involvement in the neuronal pathogenesis. Brain Res 1279:99-108. [PubMed: 19409882]  [MGI Ref ID J:157220]

Vitry S; Bruyere J; Hocquemiller M; Bigou S; Ausseil J; Colle MA; Prevost MC; Heard JM. 2010. Storage vesicles in neurons are related to Golgi complex alterations in mucopolysaccharidosis IIIB. Am J Pathol 177(6):2984-99. [PubMed: 21037080]  [MGI Ref ID J:167634]

Woloszynek JC; Coleman T; Semenkovich CF; Sands MS. 2007. Lysosomal dysfunction results in altered energy balance. J Biol Chem 282(49):35765-71. [PubMed: 17911106]  [MGI Ref ID J:129210]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129S6 background and has been backcrossed to C57BL/6J for ten generations(9/2000). The donating investigator maintains this mutant strain by backcrossing to C57BL/6 animals. Coat color expeced from breeding:Black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use


General Terms and Conditions


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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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