Strain Name: |
NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ |
|---|---|
Stock Number: |
003855 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD/Lt-Tg(Ins2-cre)5Lt/LtJ (Changed: 23-FEB-07
) |
|
NOD/Lt-Tg(Ins2-cre)5Lt (Changed: 30-AUG-06
) | |
|
NOD.RIPCreV (Changed: 30-AUG-06
) | |
| Strain Common Names | NOD.RIP-cre, line5; NOD.RIP-cre5; |
| Genes & Alleles | Ins2; cre; Tg(Ins2-cre)5Lt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Edward Leiter, The Jackson Laboratory H2 Haplotype g7 Generation N1F20p (19-FEB-06) Strain Description
This strain expresses Cre recombinase under the control of the rat insulin II promoter. Hemizygous mice carrying this transgene are phenotypically normal and overexpresss cre specifically in pancreatic beta cells. Homozygous transgenic mice experience delayed onset diabetes compared to wildtype controls. This transgenic strain is used in combination with mice carrying floxed targeted mutations to create various pancreatic beta cell-specific gene knockouts using the Cre/loxP system.Strain Development
The rat insulin II promoter driving Cre recombinase transgene was injected directly into NOD/ShiLt embryos and maintained on the NOD/ShiLt background prior to intercrossing. In 2005, the T1DR received NOD/ShiLt-Tg(Ins2-cre)5Lt at generation N1F20.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-cre)5Lt | ||
|---|---|---|---|
| Allele Name | transgene insertion 5, Edward Leiter | ||
| Common Name(s) | RIP-cre, line 5; RIPCreV; line 5; | ||
| Mutation Made By | Edward Leiter, The Jackson Laboratory | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | A construct with the rat insulin II promoter driving cre recombinase was injected into fertilized NOD/ShiLt embryos. Line 3 was established by mating to NOD/ShiLt mice prior to sibling mating. Mice overexpress Cre specifically in pancreatic beta cells. [J:124298] | ||
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
Additional Web Information
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Research Tools
Cre-lox-System (Cre-Recombinase Expression: Inducible)
Diabetes and Obesity Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesLeiter EH; Reifsnyder P; Driver J; Kamdar S; Choisy-Rossi C; Serreze DV; Hara M; Chervonsky A. 2007. Unexpected functional consequences of xenogeneic transgene expression in beta-cells of NOD mice. Diabetes Obes Metab 9 Suppl 2:14-22. [PubMed: 17919174] [J:127015]
Price and Supply Information
| Strain Name: | NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ |
| Stock Number: | 003855 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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