Strain Name:

B6.129S6-Tapbptm1Luc/J

Stock Number:

003863

Availability:

Repository-Cryopreserved

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
GenerationN7
 
Donating Investigator Luc van Kaer,   Vanderbilt University School of Medicine

Description
Mice that are homozygous null for the Tapbp gene are viable and fertile. No Tapbp gene product is detected. Tapbp is an integral component of the MHC class I peptide loading complex. Expectedly, mice that are homozygous null for tapasin fail to assemble loading complexes. Overall expression of surface MHC class I expression is reduced. Although some class I molecules are able to translocate to the cell surface, they are unable to do so in association with peptide antigen. Antigen presenting cells from null animals had a slightly reduced ability to stimulate CD8+ T cells. A more significant defect is observed in the ability to present foreign antigen. CTL responses are differentially affected. Significant defects in positive and negative intrathymic selection are also observed.

Development
A targeting construct containing a neomycin resistance gene was used to disrupt the fourth exon of the Tapbp gene. The construct was transfected into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were backcrossed to C57BL/6J mice.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tapbptm1Luc/Tapbptm1Luc

        involves: 129S6/SvEvTac * C57BL/6
  • immune system phenotype
  • abnormal lymphocyte physiology (MGI Ref ID J:122479)
    • decay of surface Kb and Db molecules on brefeldin A-treated cells is increased compared to wild-type and Arts1-null cells

Tapbptm1Luc/Tapbptm1Luc

        involves: 129S6/SvEvTac
  • immune system phenotype
  • abnormal antigen presentation via MHC class I (MGI Ref ID J:64177)
    • cells infected with an influenza virus or a vaccinia virus minigene encoding an ovalbumin peptide were poorly recognized by nucleoprotein-specific Db-restricted T cell hybridoma or by cytotoxic T lymphocytes, respectively
    • decreased level of surface class I molecules (MGI Ref ID J:64177)
      • expression is 10-15% of that observed on wild-type cells, or 2 times the level on Tap1-deficient cells
    • defective assembly of class I molecules (MGI Ref ID J:64177)
      • mice display defective assembly of class I peptide-loading complexes
      • in null cells, most heavy chains are thermolabile indicating lack of high-affinity peptides; most MHC class I molecules lack high-affinity peptides but traffic normally to the cell surface
  • abnormal cytotoxic T cell physiology (MGI Ref ID J:64177)
    • mice mount poor CTL responses to influenza virus, but normal responses to LCMV and vaccinia viruses
  • decreased CD8-positive T cell number (MGI Ref ID J:64177)
    • mice have few (15-20% of wild-type) mature CD8+ T cells in the periphery, but significantly more than Tap1-null animals
  • hematopoietic system phenotype
  • decreased CD8-positive T cell number (MGI Ref ID J:64177)
    • mice have few (15-20% of wild-type) mature CD8+ T cells in the periphery, but significantly more than Tap1-null animals
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tapbptm1Luc related

Hematological Research
Immunological Defects

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency (MHC class I deficiency)
Immunodeficiency (NK Cell Deficiency)
Immunodeficiency (T cell deficiency)

Research Tools
Immunology and Inflammation Research (MHC class I deficiency)

Virology Research
Reduced MHC Class I Responses to Specific Viruses

Genes & Alleles

Gene & Allele Information

Allele Symbol Tapbptm1Luc
Allele Name targeted mutation 1, Luc van Kaer
Allele Type Targeted (knock-out)
Common Name(s) Tpn-;
Mutation Made By Andres Grandea III,   Celltech
Strain of Origin129S6/SvEvTac
ES Cell Line NameTL1/TL-1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Tapbp, TAP binding protein
Chromosome 17
Gene Common Name(s) D17Wsu91e; DNA segment, Chr 17, Wayne State University 91, expressed; NGS17; TAPA; TPN; TPSN; Tapasin;
Molecular Note Insertion of a neomycin resistance cassette into the fourth exon. [MGI Ref ID J:64177]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Grandea AG rd; Golovina TN; Hamilton SE; Sriram V; Spies T; Brutkiewicz RR; Harty JT; Eisenlohr LC; Van Kaer L. 2000. Impaired assembly yet normal trafficking of MHC class I molecules in Tapasin mutant mice Immunity 13(2):213-22. [PubMed: 10981964]  [MGI Ref ID J:64177]

Additional References

Tapbptm1Luc related

Chen M; Tabaczewski P; Truscott SM; Van Kaer L; Stroynowski I. 2005. Hepatocytes express abundant surface class I MHC and efficiently use transporter associated with antigen processing, tapasin, and low molecular weight polypeptide proteasome subunit components of antigen processing and presentation pathway. J Immunol 175(2):1047-55. [PubMed: 16002705]  [MGI Ref ID J:100733]

Chun T; Grandea AG rd; Lybarger L; Forman J; Van Kaer L; Wang CR. 2001. Functional roles of TAP and tapasin in the assembly of M3-N-formylated peptide complexes. J Immunol 167(3):1507-14. [PubMed: 11466371]  [MGI Ref ID J:120485]

Li L; Sullivan BA; Aldrich CJ; Soloski MJ; Forman J; Grandea AG rd; Jensen PE; Van Kaer L. 2004. Differential requirement for tapasin in the presentation of leader- and insulin-derived peptide antigens to Qa-1b-restricted CTLs. J Immunol 173(6):3707-15. [PubMed: 15356116]  [MGI Ref ID J:92768]

Robbins SH; Tessmer MS; Van Kaer L; Brossay L. 2005. Direct effects of T-bet and MHC class I expression, but not STAT1, on peripheral NK cell maturation. Eur J Immunol 35(3):757-65. [PubMed: 15719366]  [MGI Ref ID J:96620]

Yan J; Parekh VV; Mendez-Fernandez Y; Olivares-Villagomez D; Dragovic S; Hill T; Roopenian DC; Joyce S; Van Kaer L. 2006. In vivo role of ER-associated peptidase activity in tailoring peptides for presentation by MHC class Ia and class Ib molecules. J Exp Med 203(3):647-59. [PubMed: 16505142]  [MGI Ref ID J:122479]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThis strain originated on a B6;129S background and has been backcrossed to C57BL/6J for at least 6 generations before being made homozygous. This strain should be housed under pathogen free conditions similar to the Tap-deficient strain (002944).

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


(3.2)